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Citations for Thermo Fisher :
8701 - 8750 of 10000+ citations for 6H 1 3 Dioxolo 4 5 g 1 benzopyran 6 one 7 8 dihydro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Supernatant was collected after centrifugation (1,500 g, 5 min) and reduced in a speedvac concentrator (Savant SPD111V; Thermo Fisher Scientific, Massachusetts, USA). EtOAc and saturated NaCl were added in equal volumes while thoroughly mixing after each addition ...
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bioRxiv - Neuroscience 2022Quote: ... The supernatant was transferred to a fresh tube, centrifuged (500 x g, 5 minutes) and the pellet was resuspended in serum-free neurobasal medium (#21103049, Thermo Fisher Scientific) with B-27 (#17504044 ...
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bioRxiv - Cell Biology 2022Quote: HEK293 and HEK293T cells were obtained from ATCC and grown at 37°C/5% CO2 in Dulbecco’s Modified Eagle Medium (4.5 g/L glucose and L-glutamine, no sodium pyruvate; Thermo Fisher Scientific Cat # 11965118) and supplemented with 10% fetal bovine serum ...
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bioRxiv - Cell Biology 2022Quote: ... 4 or 5 ug of appropriate antibodies or IgG control proteins were bound to protein G Dynabeads™ as per manufacturer instructions (Life Technologies). Equal portions of cleared lysates were added to antibody or IgG bound Dynabeads™ and incubated for 3 hours or overnight at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... The cells were centrifuged again at 500 g for 5 min and re-suspended in Hanks’ balanced salt solution (HBSS; Thermo Fisher Scientific) containing 3% HI-FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... The resulting cells were centrifuged at 500 g for 5 min and re-suspended in ammonium-chloride-potassium (ACK buffer; Thermo Fisher Scientific) to lyse red blood cells ...
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bioRxiv - Cell Biology 2023Quote: ... see the “PAA elastic gel premix” section) of the projected rigidity G’ = 50 kPa(75) with the addition of the 5% streptavidin-acrylamide (ThermoFisher, Cat# S21379) were ...
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bioRxiv - Cell Biology 2022Quote: ... Immunostained cells were then washed 2 times with DPBS for 5 minutes at room temperature and mounted on glass slides using Fluoromount G (Thermo Fisher Scientific) and a coverslip ...
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bioRxiv - Cell Biology 2023Quote: ... The resin was pelleted by centrifugation at 500 x g for 5 min and then transferred to a 900-μL spin filter (Thermo Fisher Scientific) pre-rinsed twice with 1:1 CH3OH/ddH2O ...
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bioRxiv - Cell Biology 2023Quote: ... cells were grown at 37°C/5% CO2 in Dulbecco’s Modified Eagle Medium (4.5 g/L glucose and L-glutamine, no sodium pyruvate) (Thermo Fisher Scientific, Cat. #11965118) supplemented with 10% fetal bovine serum (Sigma Aldrich ...
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bioRxiv - Evolutionary Biology 2020Quote: ... cells were pelleted by gentle centrifugation (500x g for 10 min at 4°C) in a Heraeus™ Megafuge™ 40R (ThermoFisher Scientific) and resuspended in a small volume of culture medium ...
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bioRxiv - Genetics 2020Quote: ... This mixture was incubated overnight at 4°C with beads (Protein A Dynabeads, Invitrogen, for CTCF ChIP; Protein G Dynabeads, Invitrogen, for H3K9me3 ChIP) that had been pre-blocked with 0.5% BSA and mixed with polyclonal CTCF antibody (C15410210-50 ...
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bioRxiv - Neuroscience 2020Quote: ... a centrifugation step of the whole plate at 2,000 x g for 20 min at 4 °C was indispensable (Heraeus Megafuge 40R; Thermo Fisher Scientific, Darmstadt, Germany). Indicated compounds for the injections were used in following final concentrations ...
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bioRxiv - Cell Biology 2021Quote: ... 10µg of protein was submitted to SDS-PAGE electrophoresis using NuPAGE Novex or Bolt 4-10% Bis-Tris gels (Thermo Fisher Scientific, USA). Proteins were transferred to nitrocellulose membranes using the iBlot Dry Blotting System (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... and anti-IgG (negative control) nutating overnight at 4°C followed by the addition of Dynabeads™ Protein A/G (Invitrogen, 10002D/10004D) and rocking for 4 h at 4°C ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Lysates were cleared by centrifugation at 3,700 × g for 20 min at 4°C using a microplate centrifuge (Thermo Fisher Multifuge X Pro). The supernatants were then transferred into 96-U-bottom-well assay plates (Greiner Bio-One 650101) ...
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bioRxiv - Bioengineering 2024Quote: ... The supernatant was transferred to polycarbonate tubes and centrifuged at 20,000 ×g for 45 min at 4°C to pellet MVs using a Sorvall MX 120+ micro-ultracentrifuge (Thermo Scientific, Santa Clara, CA). Next ...
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bioRxiv - Biophysics 2024Quote: ... the resulting supernatant was transferred to 1.5 ml top-opening centrifuge tubes and centrifuged for 1.5 hours at 150,000 x g at 4 °C (Sorvall MX 150+ Micro-Ultrawiring, Thermo Fisher Scientific, Waltham, MA, USA) to obtain the subpopulation of small EVs.
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bioRxiv - Molecular Biology 2024Quote: ... The chromatin suspension was centrifuged at 14,000 rpm for 15 minutes at 4°C and the supernatant incubated overnight with pre-coupled Dynabeads® (G + A, ThermoFisher Scientific, 10002D) with anti-Flag M2 antibody (F3165 ...
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bioRxiv - Neuroscience 2024Quote: ... A total of 30 μg of protein fraction for each sample was separated by electrophoresis using NuPAGETM 4-12% Bis-Tris Protein Gel (ThermoFisher Scientific, Waltham, MA). Proteins were transferred to a polyvinylidene difluoride (PVDF ...
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bioRxiv - Cell Biology 2024Quote: ... Cell lysates were clarified by centrifugation for 15 min (21.000 x g and 4 °C) and protein content in the supernatant was determined using a Pierce™ BCA assay kit (Thermo Scientific, USA). Cell lysates were diluted to a final concentration of 2 mg protein/ ml in native lysis buffer and kept on ice until used freshly.
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bioRxiv - Cell Biology 2023Quote: ... Samples were centrifuged for 10 min at 10,000 times gravity (ξ g) at 4 °C insoluble materials and total protein concentration was measured by the PierceTM BCA protein assay (Thermo Fisher Scientific). 15-20 µg of samples was prepared in Laemmli buffer and run in 8% SDS-PAGE before being transferred onto nitrocellulose membrane at 320 mA for 2.5 h ...
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bioRxiv - Biochemistry 2022Quote: ... The lysate was clarified by centrifugation at 18000 g for 35 min at 4°C and then incubated with Pierce™ Glutathione Agarose (Thermo Scientific™) equilibrated with buffer A with 1 mM TCEP at 10°C for 1 h ...
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bioRxiv - Neuroscience 2023Quote: ... 20 µg of the protein samples were then heat-denatured and subsequently separated on a 4-12% Bis-Tris gel (Invitrogen; Thermo Fisher Scientific). Separated proteins were then transferred to a 0.45 µm nitrocellulose membrane via wet blotting for 3h at 300 mA ...
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bioRxiv - Microbiology 2024Quote: ... The cells were spun down at 20,000 x g for 20 mins at 4°C in Sorvall RC6 PLUS centrifuge (Thermo Scientific, Waltham, MA, USA). The supernatant was discarded and the pellet obtained was washed with 1 ml of 10mM Tris-Cl buffer (pH-7.5 ...
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bioRxiv - Biochemistry 2024Quote: ... centrifuged at 16,000 x g for 15 minutes at 4°C and protein content determined by Pierce BCA protein assay kit (Thermo Fisher Scientific 23225). 25µg total lysate was removed as input and 0.5mg total protein was incubated with pre-washed magnetic M2 anti-FLAG (Sigma-Aldrich M8823-5ML ...
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bioRxiv - Microbiology 2020Quote: ... labeled RNAs were cooled for 5 min on ice and 1 μg yeast RNA and 10x RNA structure buffer (Ambion) were added ...
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bioRxiv - Biophysics 2021Quote: ... followed by 1 h incubation with 1:1000 anti-mouse Alexa Fluor 488 conjugated secondary antibody (diluted with PBS containing 5% BSA, Invitrogen) at RT in the dark ...
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bioRxiv - Cell Biology 2020Quote: ... sections / cells were then incubated in PBS−/− containing either DAPI (5 μg ml−1; 10 min; RT; Thermo Fisher Scientific) or DRAQ5 (1:500 ...
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bioRxiv - Cell Biology 2020Quote: 1 mg of each of the two pX335 guide RNA/SpCas9n constructs and 5 mg of the Citrine donor template were transfected into 1 million OP9 cells using Lipofectamine 2000 (Invitrogen) following the manufacturer’s protocol.
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bioRxiv - Immunology 2021Quote: OT-I T cells were isolated from spleens of CD45.1 mice and intraveneously injected (1×107 cells) either unlabeled or following CFSE labeling (5 µM, Molecular Probes) into recipient CD45.2 DC ICAM-1 KO mice and their control counterparts ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were washed three times for 5 min in PBT and incubated in Alexa Fluor 488 or 594 secondary antibodies 1:500 (Invitrogen) for 72 h at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: A gene fragment spanning from 488 base pairs upstream from the 5’UTR through 61 base pairs downstream from exon 1 was cloned into pDONR221 using a BP reaction (ThermoFisher) using the primers prkci-BP-forward 5’-GGGGACAAGTTTGTACAAAAAAGCAGGCTCctatctaggtatatgggccctc-3’ and prkci-BP-reverse 5’-GGGGACCACTTTGTACAAGAAAGCTGGGTcgcaatcctgagaataagtgaga-3’ ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were blocked in TBST with 5% milk protein and probed with the following antibodies: 1:2000 anti-Ty1 (Invitrogen), then 1:1500 goat anti-mouse IgG-HRP (Dako) ...
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bioRxiv - Genomics 2021Quote: ... and the amplified fragments were visualized by electrophoresis on agarose gels (2% for DBP1 and 1% for RBP2a) in 1x TAE buffer (40 mM Tris-acetate, 1 mM EDTA) with 5 μg/ mL ethidium bromide (Invitrogen) in a horizontal system (Bio-Rad ...
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bioRxiv - Genomics 2020Quote: ... the DNA from above was incubated with 1 μl (5 U/μl) of single-strand specific S1 nuclease (ThermoFisher Scientific) in 1x reaction buffer (40 mM sodium acetate ...
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bioRxiv - Immunology 2022Quote: HEK293-hACE2 cells15 or VeroE6-TMPRSS214 were cultured in DMEM with 10% FBS (Hyclone) and 1% PenStrep and 8ug/mL puromycin for TMPRSS2 maintenance with 5% CO2 in a 37°C incubator (ThermoFisher). One day prior to infection ...
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bioRxiv - Physiology 2022Quote: ... Coverslips were subsequently washed in quadruplicate (5 min each) with PBS and diluted secondary antibody (1:500 goat anti-rat IgG Alexa Fluor 488 conjugate, Invitrogen) was applied as above ...
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bioRxiv - Molecular Biology 2020Quote: ... 5’TYE563-labeled locked nucleic acid probes (Exiqon) against mouse major satellite sequences (Supplementary Table 5) were precipitated with mouse Cot-1 DNA (Invitrogen) and Salmon Sperm DNA (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... in Minimum essential medium (MEM), glutamine-free based Neuronal growth medium (5% fetal bovine serum, 2% B27, 1% Glutamax (100×, Invitrogen), 2 % 1 mol/L d-glucose ...
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bioRxiv - Molecular Biology 2021Quote: HEK293T cells were grown at 37 °C and 5 % CO2 in DMEM supplemented with 10% tetracycline-free fetal bovine serum and 1% Penicillin-Streptomycin (Invitrogen). Cells were transfected with the LentiX VSV-G transfection mix (Clontech ...
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bioRxiv - Molecular Biology 2021Quote: ... We labeled the purified RLC with 10 molar excess of 5-((((2-Iodoacetyl)amino)ethyl)amino)naphthalene-1-sulfonic acid (IAEDANS, Invitrogen) or dabcyl C2 maleimide (AnaSpec ...
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bioRxiv - Biophysics 2022Quote: Isolated carboxysomes were diluted to 5 mg mL-1 and denatured using 4X Bromophenol blue buffer (Fisher Scientific, United States). The samples were heated at 95°C for 10 mins ...
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bioRxiv - Immunology 2019Quote: ... In EdU-only labelling experiments mice were injected with 1 mg EdU (5-ethynyl-2’-deoxyuridine, cat #E10415, ThermoFisher Scientific) intraperitoneally and were killed one hour after injection ...
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bioRxiv - Genomics 2019Quote: ... containing 5 μl nuclease-free water with bovine serum albumin (1 mg/μl, Fermentas) and RNaseOut 20 U (Life Technologies). The plates were placed on a precooled rack and stored at −80 °C until analysis ...
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bioRxiv - Molecular Biology 2019Quote: ... were used for studies of INS-1 cells treated with CSE or AC extract with or without 5 mM N-Acetyl Cysteine (NAC) from Invitrogen; Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... 100 U/ml−1 penicillin/streptomycin and 5% (v/v) heat-inactivated fetal bovine serum (all supplements were from Invitrogen). Cells were maintained at 37°C in an atmosphere of 5% CO2 ...
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bioRxiv - Immunology 2019Quote: ... Aim2 inflammasome activation in LPS-primed J774A.1 cells was induced by transfection of 5 μg of poly(dA:dT) using Lipofectamine 2000 (Thermo Fisher) 6 h prior to sample collection [35] ...
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bioRxiv - Synthetic Biology 2020Quote: ... A type IIS assembly reaction was set up into a 0.2 mL tube (5 µL nuclease-free H2O, 1 µL 10x Tango Buffer (Thermo Fisher), 0.5 µL mg/mL bovine serum albumin (NEB) ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were washed three times with PBS for 5 min and incubated during 1 hour at RT with AlexaFluor (Invitrogen) secondary antibodies ...