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Citations for Thermo Fisher :
8801 - 8850 of 10000+ citations for 6H 1 3 Dioxolo 4 5 g 1 benzopyran 6 one 7 8 dihydro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... 0.5 mM EDTA; 0.5 mM EGTA; 1% Triton X-100; 0.5 mM PMSF; and Protease Inhibitor Cocktail [Thermo Fisher Scientific]). Protein concentration was measured using a BCA protein assay (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2020Quote: ... DNA was stained for 5 minutes in PBS with 1 ug/ml of DAPI and coverslips mounted with Pro-Long Gold (Invitrogen). Imaging was performed using a Leica SPE2 confocal microscope using LAS X version 3.3.0 software (Leica Microsystems ...
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bioRxiv - Immunology 2021Quote: ... The lysis was performed in PCR strips by transferring 5 µl of worm pellet into 45 µl of Lysis Solution supplemented with 1:100 DNase I (Ambion). The samples were then lysed by at least 10 freeze-thaw cycles using liquid nitrogen and a warm water bath ...
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bioRxiv - Genetics 2020Quote: ... Real-time quantitative PCR were performed on 1ml of diluted RT reactions (1/5) using SYBR Green kit (Life Technologies) on a One Step Plus thermocycler (Thermo Fisher) ...
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bioRxiv - Neuroscience 2021Quote: ... Slices were then incubated in the blocking/0.5 % BSA solution containing 1:500 of a secondary antibody (goat anti-chicken, Alexa Fluor 488, Invitrogen, # A-11039; ThermoFisher Scientific ...
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bioRxiv - Genomics 2020Quote: Mutagenized Aβ was then ligated into the linearized plasmid in a 5:1 ratio (insert:vector) using a ligase treatment (T4, Thermo Scientific) overnight ...
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bioRxiv - Immunology 2020Quote: ... cells were resuspended at a concentration of 1-2×106/mL in 50-100 µL of cold HBSS containing 5% heat-inactivated FBS (Gibco), 100U/mL penicillin ...
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bioRxiv - Molecular Biology 2022Quote: ... and a goat anti-rabbit secondary antibody (1:3000, SeraCare, Milford, MA) in 5% bovine serum albumin (BSA, Thermo Fisher) in TBST ...
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bioRxiv - Biochemistry 2022Quote: ... which was hydrolyzed by adding 1.5 μL of 10 units/μL T4 PNK (polynucleotide kinase) and 1 μL RNase-Out solution (40 units/μL, ThermoFisher) to the cleavage reaction above ...
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bioRxiv - Bioengineering 2022Quote: ... Next donkey-anti mouse Alexa Fluor 647 was added to the medium to a final concentration of 1:1000 and incubated on ice for 5 min (Invitrogen). 200 μL of the medium was then introduced to the cells cultured in a well of a 96 well-plate and incubated for 5 min at 37°C and 5% CO2 ...
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bioRxiv - Microbiology 2022Quote: ... the membrane was washed 3x for 5 min with TTBS and incubated 1 h at RT under agitation with anti-GFP-HRP (Thermofisher) diluted 2,500 fold in 1% BSA in TTBS ...
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bioRxiv - Developmental Biology 2022Quote: Embryos/tadpoles were either injected intra-abdominally (at stage < 41) or bathed (at later stages) with/in a 1 mM 5-ethynyl-20-deoxyuridine solution (EdU, Invitrogen). They were then fixed after the desired time-period in 4% paraformaldehyde ...
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bioRxiv - Genetics 2022Quote: ... Membranes were blocked with 5% nonfat milk and probed with mouse anti-FLAG (MA1-91878; Invitrogen; 1:1000; RRID AB_1957945), rabbit anti-GFP (NB600-308 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... cells were split with EDTA at 1:5 ratios in culture dishes coated with matrigel and culture in N2B27 medium (comprised of DMEM/F12 medium (Invitrogen) supplemented with 1% MEM-nonessential amino acids (Invitrogen) ...
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bioRxiv - Genomics 2022Quote: ZIP13K2-derived EN cells (5 x 106 /IP) were harvested and cross-linked in 1% formaldehyde (Thermo Fisher Scientific, 28908) in DPBS for 10 min at RT ...
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bioRxiv - Microbiology 2022Quote: Blood virus mixtures were titrated by ten-fold serial dilution in virus media (RPMI 1640 cell culture media supplemented with 5% fetal bovine serum [FBS] and 1% Penicillin-Streptomycin [Gibco]) in a 96-well plate before transferring dilutions to equivalent wells of a 96 well plate containing near confluent C6/36 cell monolayers ...
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bioRxiv - Neuroscience 2022Quote: Real-time PCR was performed with a 1:5 dilution of cDNA using QuantStudio3 real-time PCR apparatus (Life Technologies) and TaqMan® reagents with predesigned assays for the target genes GAD65 (alias GAD2 ...
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bioRxiv - Synthetic Biology 2022Quote: ... 5 μl of the Phire PCR reaction was verified on a 1% agarose gel (Ultra-Pure agarose, # 16500-500, Invitrogen), with a 1kb bench top ladder (G754A ...
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Allelic diversity uncovers protein domains contributing to the emergence of antimicrobial resistancebioRxiv - Microbiology 2022Quote: ... Samples were diluted 1:5 in sample loading buffer and quantified using the Pierce BCA Protein Assay Kit (Thermo Scientific). Lysates were normalized to 100μg/mL in 1X Bolt™ LDS Sample Buffer (Invitrogen) ...
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bioRxiv - Microbiology 2022Quote: ... Samples were then blocked with 5% FBS in PBS for 20 min before stained with Alexa Flour 594 phalloidin (1:100, Invitrogen) for 1 h and 10 μg/ml DAPI (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: Normal adult donor blood (in EDTA) was diluted 1:5 with RPMI 1640 supplemented with pen/strep and 2mM L-glutamine (Gibco). Cells were incubated at 37°C for 2 hours in the presence of polymyxin B (1.25μg/ml ...
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bioRxiv - Evolutionary Biology 2022Quote: ... iPSCs were split with EDTA at 1:5 ratios in culture dishes coated with matrigel and culture in N2B27 medium (comprised of DMEM/F12 medium (Invitrogen) supplemented with 1% MEM-nonessential amino acids (Invitrogen) ...
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bioRxiv - Neuroscience 2022Quote: ... 1 ng-5 μg of RNA per reaction was used in SuperScript™ First-Strand synthesis kits (Invitrogen, ID: 11904018). Nucleic acid purity was assessed using a NanoPhotometer® (Implen).
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bioRxiv - Synthetic Biology 2022Quote: ... A 1:10 dilution was performed and 2μl of diluted DNA was amplified by mean of a QuantStudio 5 (Applied Biosystems) thermal cycler using the SYgreen Blue Mix (PCRbio ...
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bioRxiv - Microbiology 2022Quote: ... the robot adds 2 volumes of 30% PEG 6000 (in 1.6 M NaCl) and 35ul of 1:5 diluted Glycoblue (Invitrogen, Thermo Fisher) to each well ...
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bioRxiv - Physiology 2024Quote: Mouse pancreata or human and mouse islet sections were then washed with PBS for 5 minutes and permeabilized and blocked with 1% normal donkey serum and 0.15% Triton X-100 (Fisher Scientific) in PBS for 1 hour ...
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bioRxiv - Synthetic Biology 2024Quote: ... Blots were washed with TBST 3x for 5 min and incubated in corresponding secondary antibody for 1 hour room temperature: goat anti-rabbit antibody (Invitrogen) or rabbit anti-mouse antibody (Invitrogen) ...
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bioRxiv - Biophysics 2024Quote: ... to which 2.5 μL of ddH2O and 2.5 μL of reverse transcription (RT) mix (1 μL 5X First strand buffer (Thermo Fisher), 0.01 M DTT ...
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bioRxiv - Neuroscience 2024Quote: ... Brain sections were washed three times for 5 minutes in PBS and incubated for 1 hour in blocking solution [10% normal goat serum (NGS; #50197Z, Invitrogen), 0.1% Triton X-100 in PBS] ...
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bioRxiv - Cell Biology 2023Quote: ... 40 cycles of 95°C for 1 s and 60°C for 20 s on the QuantStudio 5 Real-Time PCR System (ThermoFisher). Relative quantification of each gene was done by QuantStudio Design&Analysis v1.5.1 software (ThermoFisher ...
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bioRxiv - Neuroscience 2023Quote: ... were conducted to remove trace PFA followed by 5 min antigen retrieval with SDS (1% Sodium Dodecyl Sulfate; Fisher Scientific) in PBS ...
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bioRxiv - Microbiology 2023Quote: Purified DNA was used as a template for qPCR reactions by mixing 1 μl of DNA to 5 μl of 2X SYBR Select Master Mix (ThermoFisher), 3.6 μl of nuclease-free water ...
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bioRxiv - Molecular Biology 2024Quote: ... Digestions were incubated for 1 hour at 37 ℃ and then diluted 1:5 with UltraPure water before loading on E-gel EX 2% Agarose gels (Invitrogen). Backbones were purified using the Monarch Gel Extraction Kit (Monarch) ...
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bioRxiv - Neuroscience 2024Quote: ... slices were washed in PBS three times (5 mins) and incubated in the secondary antibodies Alexa Fluor donkey anti goat 488 (1:500, cat# A-11055, Invitrogen), diluted in blocking solution for two hours at room temperature ...
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Mitochondrial bioenergetics stimulates autophagy for pathological tau clearance in tauopathy neuronsbioRxiv - Neuroscience 2024Quote: ... cortical neurons were resuspended in a plating medium (5% FBS, insulin, glutamate, G5 and 1 × B27) supplemented with 100 × L-glutamine in Neurobasal medium (Invitrogen) and plated at a density of 100,000 cells per cm2 on polyornithine- and fibronectin-coated coverslips (Carolina Cover Glasses ...
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bioRxiv - Neuroscience 2024Quote: ... Dissociated neurons were plated and allowed to adhere for 2 hours at 37°C in 5% CO2 wells were flooded with DMEM/F12 plus GlutaMAX culture media containing 1% penicillin/streptomycin (15070063, Thermofisher), 10 ng/mL mouse nerve growth factor (01-125 ...
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bioRxiv - Microbiology 2023Quote: ... Staining of DNA was performed on fixed cells by incubating them for 5 min in a 1 μg/ml 4,6-diamidino-2-phenylindole (DAPI, 62248, Thermo Fisher) solution ...
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bioRxiv - Microbiology 2024Quote: ... PCR products were visualized by electrophoresis on 0.5% Tris-acetate-EDTA (TAE) agarose gel at 100 V for 1 h using SYBR Safe DNA Gel Stain (Invitrogen).
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bioRxiv - Neuroscience 2023Quote: ... Slides were then washed three times in 1x PBS for 5 minutes and countered stained with DAPI (1:20,000, Thermo Fisher). Slides were coverslipped using ProLong™ Gold Antifade Mountant (Thermo Fisher) ...
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bioRxiv - Developmental Biology 2023Quote: ... cells were incubated in blocking buffer (PBS with 5% BSA) for 30 min and incubated with Alexa Fluor-568-conjugated donkey anti goat secondary antibodies (1:1000, Invitrogen) in PBS with 2.5% BSA for 1 h at RT ...
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bioRxiv - Biochemistry 2024Quote: ... and HFF-1 cells were maintained at 37°C under 5% CO2 atmosphere in RPMI-1640 medium (ThermoFisher, for HeLa) and DMEM (ThermoFisher ...
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bioRxiv - Biochemistry 2024Quote: ... was labeled with the fluorescent probe 5-((((2-iodoacetyl)amino)ethyl)amino)naphthalene-1-sulfonic acid (1,5-IAEDANS from Molecular Probes) as previously described (17 ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... using standards covering a range from 10-1 to 10-5 ng/µl fungal DNA (measured using a NanoDrop spectrophotometer, Thermofisher). Each run included the standards and a negative control ...
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bioRxiv - Bioengineering 2024Quote: ... and splenocyte to resuspend post centrifugation for 5 minutes and subsequently diluted with 5mL ice-cold FACS Buffer (2 or 10% FBS in 1× HBSS (Gibco) or 1X PBS ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were incubated with secondary antibodies diluted 1:1000 in 5% BSA in PBST (anti-mouse Alexa Fluor 555, CST 4409S; anti-mouse Alexa Fluor 647, Invitrogen, A-21235 ...
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bioRxiv - Biochemistry 2023Quote: ... The chamber was subsequently incubated for 5 minutes with 50 µL of 50 µg mL-1 of NeutrAvidin (Life Technologies) in casein buffer ...
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bioRxiv - Bioengineering 2024Quote: ... 300 g Bloom type A gelatin which was dissolved at 15% w/v 70 °C for 1 h was diluted to 5% w/v using DMEM/F12 with HEPES (Gibco) and supplemented with CaCl2 to a final concentration of 2.5 mM and 5 U mL-1 thrombin ...
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bioRxiv - Cancer Biology 2024Quote: ... NCI-H1048 were grown in HITES medium (DMEM media supplemented with 5% FBS, 1% penicillin/streptomycin, Insulin-Transferrin-Selenium (Gibco), 10 nM Hydrocortisone (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2023Quote: ... 1 μL of dNTPs (10 mM) and 0.5 μL of Platinum Taq DNA polymerase (5 U/ml) (Invitrogen, Marseille, France) and 1 μL of forward and reverse primer (20 μM) ...
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bioRxiv - Microbiology 2023Quote: ... The infection was incubated for 2-3 hours at 37°C and then the viral inoculum was removed and replaced with a pH 7.2 methyl cellulose overlay medium (1% methyl cellulose [Sigma-Aldrich, MA]/5% FBS [Gibco, MA]/1X penicillin-streptomycin [Gibco ...