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Citations for Thermo Fisher :
9101 - 9150 of 10000+ citations for 6H 1 3 Dioxolo 4 5 g 1 benzopyran 6 one 7 8 dihydro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... which was bound to Protein G-coupled magnetic beads (Dynabeads Protein G, Invitrogen). Precipitated proteins were resolved in 8% SDS-PAGE gels ...
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bioRxiv - Biochemistry 2022Quote: ... 0.5 μL pre-washed magnetic-conjugated protein G beads (Dynabeads protein G, Invitrogen) were added to the reactions followed by the incubation for 1 hour at 4°C in the thermomixer (950 rpm) ...
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bioRxiv - Immunology 2020Quote: ... 30 µL of protein G coated magnetic beads (Invitrogen, Dynabeads™ Protein G) were added and the mix was incubated for additional 30 min on a rotating mixer at RT ...
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bioRxiv - Immunology 2020Quote: ... We pre-coupled 200 μl Protein-G beads (10004D, Dynabeads Protein G, Invitrogen) with 20 μg antibody (anti-GFP ...
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bioRxiv - Neuroscience 2022Quote: ... Protein G-coated Magnetic Beads (Dynabeads Protein G, Cat# 10004D, Thermo Fisher Scientific) were incubated with recombinant NLGN1-Fc ...
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bioRxiv - Molecular Biology 2019Quote: ... YSM tissues from day 5 embryos were treated with collagenase (collagenase type 4, 17104019, ThermoFisher, Waltham, MA, USA) to partially digest the extracellular matrix and facilitate cell isolation [17] ...
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bioRxiv - Genomics 2019Quote: ... and washed in Buffer F (50 mM Tris-HCl pH 8.0, 40% glycerol, 5 mM MgCl2, 0.5 mM DTT, 4 u/mL RNase inhibitor [SUPERaseIN, Ambion]). Washed permeabilized cells were finally resuspended in Buffer F at a density of 1×106 cells/30 μL and immediately frozen in liquid nitrogen ...
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Insights into the secondary structural ensembles of the full SARS-CoV-2 RNA genome in infected cellsbioRxiv - Biochemistry 2021Quote: ... Infected cell pellets were centrifuged at 5000xg for 5 min at 4 °C and resuspended in Trizol (Ambion).
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bioRxiv - Cancer Biology 2021Quote: ... Samples were then stained with 5 µL FITC anti-human DR4 (Thermo Fisher Scientific, Clone DR-4-02) for 30 min at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... the Ca+2 indicator Fluo-4-AM (Molecular Probes, Eugene, OR; 2–5 µl of 2 mM dye) were dropped over S1 cortex ...
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bioRxiv - Neuroscience 2021Quote: ... the washing medium was removed and 80 L of 5 M cell permeant Fluo-4 AM (Life technologies) in neurobasal medium was added to wells at room temperature and incubated at 37 °C for 30 minutes ...
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bioRxiv - Cancer Biology 2019Quote: ... harvested after 4-5 days and counterstained with Near-IR fluorescent Dye (Live/Dead Cell Stain Kit, Invitrogen) to determine the number of viable dividing (CFSE/NearIR- ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 4 mM DTT and 20% glycerol) at 30 °C in a Cytation 5 imaging reader (Thermo Fisher Scientific) with filters for excitation at 360/40 nm and emission at 460/40 nm ...
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bioRxiv - Cell Biology 2021Quote: ... 5-10µg of protein per sample was run on a 4%–12% NuPAGE Bis-Tris Gel (ThermoFisher Scientific) and then transferred to PVDF membrane by liquid transfer using NuPAGE Transfer buffer (ThermoFisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were boiled for 5 minutes then electrophoresed in pre-cast 4-12% NuPAGE Bis-Tris Gels (Invitrogen). Following transfer ...
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bioRxiv - Cell Biology 2024Quote: ... 5% v/v 2-mercaptoethanol) and fractionated by 4–12% Bis-Tris gel (Thermo Fisher Scientific Cat#NP0335BOX) using MES running buffer (Thermo Fisher Scientific Cat#NP000202) ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were further lysed via sonication (10 sec on 5 sec off, repeat 3x, ThermoFisher: FB120, 4°C) and protein was clarified by centrifugation at 21,000 x g for 5 min at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... 5 minutes) at 4 °C and re-suspended in media composed of Minimal Essential Medium (MEM, Life Technologies) with 10%/vol fetal bovine serum (Omega) ...
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bioRxiv - Neuroscience 2023Quote: ... incubated for 5 min at 95°C and loaded onto a NuPAGE 4-12% Tris/Bis gel (ThermoFisher) with NuPAGE MOPS running buffer (ThermoFisher) ...
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bioRxiv - Cancer Biology 2023Quote: ... and 5% 2-mercaptoethanol] and was resolved by SDS-PAGE on 4% to 12% Bis-Tris gels (Invitrogen). Proteins were transferred to a polyvinylidene difluoride immobilon TM-P membrane (Millipore ...
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bioRxiv - Immunology 2023Quote: ... at 95°C for 5 min and separated on a 4-12% Bis-Tris gel (NuPAGE, Thermo Scientific) for 60 min at 170V ...
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bioRxiv - Cell Biology 2022Quote: ... 5–10mg of protein per sample was run on a 4–12% NuPAGE Bis-Tris Gel (ThermoFisher Scientific) and then transferred to PVDF membrane by liquid transfer using NuPAGE Transfer buffer (ThermoFisher Scientific) ...
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bioRxiv - Bioengineering 2023Quote: Protein samples (5 µg protein) were mixed with either 4 x LDS sample buffer (Thermo-Fisher Life Technologies) or 4x LDS sample buffer + 50 mM dithiothreitol for SDS-PAGE analysis under non-reducing or reducing conditions ...
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bioRxiv - Genomics 2023Quote: ... then heated at 100°C for 5 minutes and loaded onto a 4-12% polyacrylamide gel (ThermoFisher NP0322). Transfer onto a PVDF membrane was performed in 1X NuPage Transfer Buffer (ThermoFisher NP00061 ...
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bioRxiv - Microbiology 2024Quote: ... Gametocyte cultures were seeded from asexual blood stage cultures at 2% parasitaemia and 4% haematocrit in gametocyte medium (RPMI-1640 with 5% human AB+ serum, 0.5% Albumax, 3.7% 100xHT supplement (ThermoFisher), 30 mg/ml L-glutamine ...
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bioRxiv - Microbiology 2024Quote: ... Membranes were washed 4 times before staining for 5 minutes with Pierce ECL Western Blotting Substrate (Thermo Fisher) as per the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... cells were resuspended in 100 µL of PBS containing 5 µg/mL FM™ 4-64 (Molecular Probes). After incubation for 5 min at room temperature in the dark ...
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bioRxiv - Immunology 2024Quote: ... we injected the mice intraperitoneally with 4 mg/ml of 5-ethynyl-2′-deoxyuridine (EdU) (Invitrogen, Waltham, MA) with the goal of labeling circulating monocytes to assess macrophage retention among groups ...
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bioRxiv - Synthetic Biology 2021Quote: ... An imaging glass chamber was incubated for 5 min with a mix of bovine serum albumin (BSA) and BSA-biotin (1:1 molar ratio, 1 mg mL−1, Thermo Fisher Scientific), followed by incubation with Neutravidin (1 mg mL−1 ...
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bioRxiv - Neuroscience 2021Quote: ... cells were pre-incubated with “1:1 medium” composed of a 1:1 mixture of mTeSR1 medium and “hPSC medium” [hPSC medium: KO DMEM (Gibco 10829-018) with 20% KOSR (Gibco 10828-028) ...
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bioRxiv - Bioengineering 2021Quote: ... Isolated CD3+ T cells were activated at 1×106 cells mL−1 in a 1:1 ratio with CD3/CD28 magnetic dynabeads (CTS, Thermo Fisher Scientific), 100 U mL−1 of IL-7 ...
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bioRxiv - Plant Biology 2019Quote: ... Supernatants were collected from the cultures at a cell density of 1·107 cells·ml−1 and mixed in a 1:1 ratio with AIM-V medium (Life Technologies, NY, USA). Commercially available recombinant growth factors were used as positive controls at the following concentrations ...
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bioRxiv - Synthetic Biology 2020Quote: ... The sample reservoir of the imaging chamber was functionalized with 1:1 molar ratio of bovine serum albumin (BSA) and BSA-biotin (1 mg mL−1, Thermo Fisher Scientific), and then with Neutravidin (1 mg mL−1 ...
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bioRxiv - Neuroscience 2022Quote: ... stained with primary antibody (rabbit anti-iba-1, 1:1000, Wako, #019- 19741; mouse anti-NeuN, 1:1000, Abcam, #ab104224; Hoechst, 1:1000, Thermo Fisher, H1399) overnight at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... The membrane was then blocked using 5% w/v non-fat dry milk in TBST for one hour at room temperature and then incubated with primary antibody (anti-Alix, 1:5000; Protein Tech, 12422-1-AP or anA-FloAllin-1, 1:5000; Fisher Scientific, BDB610820) at 4°C overnight ...
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bioRxiv - Microbiology 2023Quote: ... and undigested chromatin were adjusted to 810μl with MCN-digestion buffer with 0.005M EGTA and cross-linked by adding 135 μl of buffered 7 % formaldehyde solution (7 % CH2O, 140 mM Na2HPO4 pH 8.67) using 16 % CH2O methanol-free ampules (Thermo Scientific Cat #28906), to a final concentration of 1 % ...
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bioRxiv - Biophysics 2021Quote: ... at a ratio of 1:1.5:1.75:2 (FAM155A-3×FLAG:NALCN-1077-3×HA-GFP:UNC79-3×FLAG:UNC80-3×FLAG) using Lipofectamine 3000 (Thermo Fisher Scientific) and incubated for 40-48 hours ...
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bioRxiv - Cancer Biology 2021Quote: ... 1% PSQ and 1% sodium pyruvate (Gibco 11360-070) at 37°C and 5% CO2 ...
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bioRxiv - Cancer Biology 2021Quote: ... DMEM/F-12 1:1 Medium (Gibco, 11320-074) with 10% FCS and 1% L-glutamine.
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bioRxiv - Cell Biology 2020Quote: ... 1:1 ratio of DMEM-F12 (Invitrogen #10565-018) and Neurobasal media (Invitrogen #21103-049 ...
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bioRxiv - Genetics 2021Quote: ... and ZO-1 (Life Technologies, 61-7300, 1:200) in blocking solution overnight at 4°C ...
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bioRxiv - Immunology 2019Quote: ... containing 1 mg/ml collagenase Type 1 (Life Technologies), 0.4 units Dispase (Life Technologies) ...
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bioRxiv - Biophysics 2019Quote: ... 1% L-glutamin and 1% penicillin/streptomycin (Life Technologies) at 37°C and 5% CO2 in a cell culture incubator ...
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bioRxiv - Neuroscience 2019Quote: ... 1:1 Fluorescein and rhodamine maleimide (ThermoFisher Scientific, USA) labeled α-Syn containing single liquid droplet was imaged using a home-built epifluorescence total internal reflection fluorescence microscopy (Nikon Eclipse TE2000-U) ...
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bioRxiv - Genetics 2021Quote: ... DLG-1 (1:500; Thermo Fisher Scientific MA1-045).
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bioRxiv - Developmental Biology 2021Quote: ... and anti-ZO-1 (1/50, 33-9100, Invitrogen). For immunostaining of Laminin and Fibronectin ...
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bioRxiv - Cell Biology 2021Quote: ... 1% L-glutamine (Hyclone) and 1% penicillin/streptomycin (Gibco) and Leukemia inhibitory factor (LIF ...
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bioRxiv - Immunology 2021Quote: ... PU.1 (Invitrogen Cat #MA5-15064, 1:500-1000), NS1 (Invitrogen Cat #PIPA532243 ...
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bioRxiv - Neuroscience 2020Quote: ... ALPL (702454, Invitrogen, 1:100 primary, 1:1,000 secondary), and CD11b (ab 133357 Abcam ...
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bioRxiv - Cell Biology 2020Quote: ... and 1 U μL−1 HinFI (Thermo Fisher Scientific). mRNA:cDNA hybrids were purified using Agencout AMPure XP beads (Beckman Coulter Genomics ...