Labshake search
Citations for Thermo Fisher :
8851 - 8900 of 10000+ citations for 6H 1 3 Dioxolo 4 5 g 1 benzopyran 6 one 7 8 dihydro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... REF52 cells were grown in six-well plates to 60% confluency and transfected with 1µg of the vector together with 1 µl of Lipofectamine 3000 and 5 µl of P3000 solution (ThermoFisher Scientific). Two days post transfection cells were detached ...
-
bioRxiv - Cancer Biology 2023Quote: ... Horseradish-peroxidase conjugated secondary antibodies diluted 1:5000 in 5% non-fat milk were used and visualized with SuperSignal West Pico or Femto substrate kits (ThermoFisher). The following commercial primary antibodies supplemented with 0.02% sodium azide were used for immunoblot analysis ...
-
bioRxiv - Neuroscience 2023Quote: ... The total RNA concentration was detected using a Thermo Scientific MultiskanGO Microplate Spectrophotometer and 1 μg was immediately retro-transcribed using a mixture containing: 5× first strand buffer (18080-044, Invitrogen,), 2.5 μM of random hexamers (N112470 ...
-
bioRxiv - Neuroscience 2023Quote: ... or DIV12 (overexpression) primary hippocampal neurons were transfected with 1-2 μg plasmid DNA and 5 μL Lipofectamine 2000 (Invitrogen) according to manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... fixed brains were blocked in 5% NGS then incubated overnight with the primary antibodies anti-GFP (1:100, Invitrogen: G10362), anti-Fas2 [1:25 ...
-
bioRxiv - Microbiology 2023Quote: ... and immortalised HFF cells (ATCC CRL-4001) were grown at 37°C with 5% CO2 in DMEM medium supplemented with 10% FBS and 1% Glutamax (Gibco). Gold grids with SiO2 film (Quantifoil ...
-
bioRxiv - Cell Biology 2023Quote: ... the cells were subjected to selection media (DMEM-GlutaMax plus 5% FBS) containing 400 μg mL−1 of Geneticin (Invitrogen). The growth of single cell-derived colonies was monitored over two weeks ...
-
bioRxiv - Pathology 2023Quote: ... 24 hours after plating medium was replaced by Basal medium supplemented with 5 ng/ml EGF and 1/100 dilution of Insulin-Transferrin-Sodium Selenite (ITS) Supplement (Invitrogen) and 3 % of fresh Matrigel ...
-
bioRxiv - Genomics 2023Quote: ... 0.5-1 µg of RNA was reverse-transcribed with random hexamer primers using SuperScript III reverse transcriptase (18080-051; Invitrogen) according to the manufacturer’s protocols ...
-
bioRxiv - Animal Behavior and Cognition 2023Quote: ... slices containing the dorsal hippocampus were washed for 5 mins with 1X PBS three times before staining the slices for 10 minutes with DAPI (1:1,000, Life Technologies) and mounted on slides with Vectashield (Vector Labs) ...
-
bioRxiv - Neuroscience 2023Quote: ... were conducted to remove trace PFA followed by 5 min antigen retrieval with SDS (1% Sodium Dodecyl Sulfate; Fisher Scientific) in PBS ...
-
bioRxiv - Neuroscience 2023Quote: ... and the nuclei pellet was resuspended in 2 mL of ice-cold resuspension buffer (1 M HEPES [#J16924- AE, ThermoFisher Scientific]; 5 M NaCl [#01094764, ThermoFisher Scientific] ...
-
bioRxiv - Bioengineering 2023Quote: ... from IDT and 2.5µL of the diluted cDNA (1:5 dilution) in a 10µL reaction with Fast SYBR Green Master Mix (Life Technologies) and a LightCycler® 480 Real-time PCR System (Roche ...
-
bioRxiv - Biochemistry 2023Quote: ... the buffer was exchanged to HBS buffer (1×HBS pH 7.2, 5% (v/v) glycerol) by gel filtration (Zeba Spin Desalting column, 2 mL, Thermo Fisher). For immunoblotting ...
-
bioRxiv - Biochemistry 2022Quote: ... Tissue culture was performed at 37°C in a 5 % CO2 humidified incubator using 1 ml of DMEM media supplemented with 20 % FBS (GIBCO), 100 U/ml penicillin (Invitrogen ...
-
bioRxiv - Genetics 2023Quote: ... The nuclear extract was denatured at 95-100°C for 5 minutes in 1× NuPAGE LDS sample buffer (ThermoFisher, NP0007) containing 10% v/v β-Mercaptoethanol (Millipore Sigma ...
-
bioRxiv - Molecular Biology 2023Quote: ... Blots were incubated for 5 minutes with 1 ml of Supersignal West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific, 34095) and imaged using the ‘chemi high-resolution’ setting on a Bio-Rad ChemiDoc MP System.
-
bioRxiv - Molecular Biology 2023Quote: ... Blots were incubated for 5 minutes with 1 ml of Supersignal West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific, 34095) and the final blot were imaged using the ‘chemi high-resolution’ setting on a Bio-Rad ChemiDoc MP System.
-
bioRxiv - Bioengineering 2023Quote: ... cells were washed with PBS and incubated with 1 µg/mL Hoechst 33342 (Thermo) and 5 μg/mL Wheat Germ Agglutinin Oregon Green 488 Conjugate (Invitrogen) for 10 min at 37 °C ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... washed again two times in 1XPBS 5’ each and incubated with Alexa Fluor Tyramide 594 (1:100, Alexa Fluor 594 Tyramide SuperBoost Kit, Invitrogen, Thermo Fisher Scientific ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... washed three times in 1XPBS for 5’ each and incubated for 10’ at RT with Hoechst (1:1000, in 1XPBS, Invitrogen, Thermo Fisher Scientific ...
-
bioRxiv - Genetics 2023Quote: ... were enzymatically and mechanically generated and cultured in suspension in the presence of BMP4 (1 ng/mL) and ROCK inhibitor (5 µM) for 24 hours in StemPro-34 media (Gibco) supplemented with L-glutamine (Gibco) ...
-
bioRxiv - Developmental Biology 2023Quote: ... at RT for 10 min and blocked at RT for 1 hr in blocking solution (5% NBCS, 2x antibiotic-antimycotic (Gibco), 0.1% sodium azide (Sigma s2002-100G) ...
-
bioRxiv - Microbiology 2023Quote: ... Cells were washed again in 0.1% PBS-Triton and the nucleic acids were labeled with 1 µg/mL DAPI for 5 minutes before cells were mounted on a slide with ProlongGold (ThermoFisher). Fluorescence images were taken using a confocal fluorescence microscope (Zeiss ...
-
bioRxiv - Microbiology 2023Quote: ... supplemented with 10% fetal bovine serum (FBS) and 1% penicillin and streptomycin] in a 37 °C incubator with 5% CO2 [all reagents were purchased from Gibco, Thermo Fisher Scientific (Waltham ...
-
bioRxiv - Bioengineering 2023Quote: ... cells were stained for 10 min in 0.5 µL/mL calcein-AM and 2 µL/mL ethidium homodimer-1 from the Live/Dead assay kit (L3224, Invitrogen). Then ...
-
bioRxiv - Molecular Biology 2023Quote: ... DNA was precipitated in 5 ml Eppendorf tubes using 1 ml of isopropanol with 3.7 μl GlycoBlue (Thermo Fisher Scientific) and incubating at −80°C overnight ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were resuspended to a final concentration of 5 x 106 cells/ml and labelled with 1 µM CTV (Invitrogen) in PBS for 10 min at 37LJ°C ...
-
bioRxiv - Biophysics 2024Quote: ... to which 2.5 μL of ddH2O and 2.5 μL of reverse transcription (RT) mix (1 μL 5X First strand buffer (Thermo Fisher), 0.01 M DTT ...
-
bioRxiv - Biochemistry 2023Quote: ... The chamber was subsequently incubated for 5 minutes with 50 µL of 50 µg mL-1 of NeutrAvidin (Life Technologies) in casein buffer ...
-
bioRxiv - Immunology 2023Quote: ... A total of 5 × 106 cells were resuspended in 500 μl of cell culture medium with 5 μg/ml of Indo-1 AM and 0.5 μg/ ml of pluronic F-127 (both from Life Technologies) and incubated in the dark for 30 min at 37 °C ...
-
bioRxiv - Microbiology 2023Quote: ... At 72 hours media was replaced with complete growth media containing 5 µg.mL-1 of Puromycin Dihydrochloride for selection (Gibco, A1113803). Following complete death of non-transduced cells ...
-
bioRxiv - Biophysics 2023Quote: ... 300 mL NaCl, 5 mM imidazole, 10% glycerol, 0.2 mM 2-mercaptoethanol, 1 mM PMSF, and Pierce Protease Inhibitor Tablets [Thermo Fisher]). Cells were sonicated for 5 min (30% amplitude ...
-
bioRxiv - Bioengineering 2023Quote: ... HeLa cells were maintained at 37 °C in a humidified incubator with 5% CO2 and passaged at 70–80% confluency using 1× TrypLE Express (Gibco).
-
bioRxiv - Cell Biology 2023Quote: ... 40 cycles of 95°C for 1 s and 60°C for 20 s on the QuantStudio 5 Real-Time PCR System (ThermoFisher). Relative quantification of each gene was done by QuantStudio Design&Analysis v1.5.1 software (ThermoFisher ...
-
bioRxiv - Neuroscience 2023Quote: ... Slides were then washed three times in 1x PBS for 5 minutes and countered stained with DAPI (1:20,000, Thermo Fisher). Slides were coverslipped using ProLong™ Gold Antifade Mountant (Thermo Fisher) ...
-
bioRxiv - Bioengineering 2024Quote: ... 300 g Bloom type A gelatin which was dissolved at 15% w/v 70 °C for 1 h was diluted to 5% w/v using DMEM/F12 with HEPES (Gibco) and supplemented with CaCl2 to a final concentration of 2.5 mM and 5 U mL-1 thrombin ...
-
bioRxiv - Cancer Biology 2024Quote: ... NCI-H1048 were grown in HITES medium (DMEM media supplemented with 5% FBS, 1% penicillin/streptomycin, Insulin-Transferrin-Selenium (Gibco), 10 nM Hydrocortisone (Sigma-Aldrich) ...
-
bioRxiv - Neuroscience 2023Quote: ... were conducted to remove trace PFA followed by 5 min antigen retrieval with SDS (1% Sodium Dodecyl Sulfate; Fisher Scientific) in PBS ...
-
bioRxiv - Developmental Biology 2023Quote: ... cells were incubated in blocking buffer (PBS with 5% BSA) for 30 min and incubated with Alexa Fluor-568-conjugated donkey anti goat secondary antibodies (1:1000, Invitrogen) in PBS with 2.5% BSA for 1 h at RT ...
-
bioRxiv - Microbiology 2023Quote: Purified DNA was used as a template for qPCR reactions by mixing 1 μl of DNA to 5 μl of 2X SYBR Select Master Mix (ThermoFisher), 3.6 μl of nuclease-free water ...
-
bioRxiv - Developmental Biology 2023Quote: ... Membranes were blocked in 5 % BSA in TBS-T for 1 0.2 µg/ml Streptavidin-HRP (S911, Thermo Fisher Scientific) in 5 % BSA in TBS-T at 4 °C overnight ...
-
bioRxiv - Physiology 2024Quote: ... the samples were denatured for 5 min at 95°C with 1× Nupage sample reducing agent (#NP0004, Thermo Fisher Scientific) and 1× Nupage LDS sample buffer (#NP0007 ...
-
bioRxiv - Genomics 2024Quote: ... DNA pellets were resuspended in 0.6 ml TE and treated with 5 μl of RNaseA (10 mg ml−1, Thermo Scientific) for 30 min at 37 °C ...
-
bioRxiv - Cell Biology 2024Quote: ... The slides were washed three times with PBS and blocked with 5% milk inside a humid chamber for 1 h before being incubated with anti-V5 mouse primary antibody (Invitrogen) (1:400 dilution ...
-
bioRxiv - Cancer Biology 2024Quote: ... We then incubated membranes with secondary antibody (in 5% Blocking Buffer) for 1 hour at room temperature (Invitrogen cat. A16110; Invitrogen cat ...
-
bioRxiv - Cancer Biology 2024Quote: T cells from KOG mouse TDLNs (5 × 105) were co-cultured with SIINFEKL peptide (1 μg/mL, Thermo Fisher Scientific) in a 96-well plate (Thermo Fisher Scientific) ...
-
bioRxiv - Cell Biology 2024Quote: ... and 10 mM L-glutamine at 37°C and in 5% CO2. Human RPE-1 cells (a gift from Dr. Hongtao Yu) were cultured in DMEM: F-12 medium (Invitrogen) supplemented with 10% FBS and 10mM L-glutamine at 37°C and in 5% CO2 ...
-
TAK1 operates at the primary cilium in non-canonical TGFB/BMP signaling to control heart developmentbioRxiv - Developmental Biology 2024Quote: RPE-1 (human telomerase reverse transcriptase-immortalized retinal pigmented epithelial-1) cells were cultured in T75 flasks at 37°C and 5% CO2 in DMEM (Gibco) supplemented with 10% fetal bovine serum (FBS ...
-
bioRxiv - Cancer Biology 2024Quote: ... 5% non-fat milk powder) and then incubated with primary antibody against ERα (ThermoFisher #MA5-14104, 1:1000 in BS), EHMT2/G9a (Abcam #ab133482 ...