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Citations for Thermo Fisher :
8751 - 8800 of 10000+ citations for 6H 1 3 Dioxolo 4 5 g 1 benzopyran 6 one 7 8 dihydro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... Labeled DNA (500 ng) was precipitated during 30 min incubation at −20°C after adding 5 μl of mouse Cot-1 DNA (Invitrogen), 0.5 volume of 7.5 M ammonium acetate and 2.5 volumes of cold 100% ethanol ...
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bioRxiv - Molecular Biology 2020Quote: ... 5 µl of 10X TURBO™ DNase Buffer and 1 µl of TURBO™ DNase (Art. No. AM2238, Invitrogen™) were added to the RNA and incubated at 37 °C for 30 minutes with gentle shaking ...
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bioRxiv - Microbiology 2019Quote: ... burnetii total RNA extracted from LCVs (3dpi) infecting Vero (carA and metK) or THP-1 (cvpD) cells using a 5’ RACE System kit (Invitrogen) according to manufacturer protocols and with gene-specific primers (see S9 Fig) ...
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bioRxiv - Immunology 2019Quote: Annealed oligonucleotides containing the sequence of the HCV 5’ppp poly-U/UC region (Table 1) (20) were in vitro transcribed using the Megashortscript T7 transcription kit (Ambion) followed by ethanol precipitation ...
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bioRxiv - Bioengineering 2021Quote: ... Samples were analyzed using an Agilent 1100 HPLC system equipped with a MAbPac SEC-1 analytical column (4.0 × 300 mm, 5 µm, Thermo Scientific). The conditions were as follows ...
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bioRxiv - Biochemistry 2021Quote: ... Unbound primary antibodies were removed by washing four times for 5 min in PBS-T at room temperature followed by incubation with secondary antibodies (Alexa Fluor 546; 1:1000; Invitrogen) and DAPI for 45 min ...
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bioRxiv - Cell Biology 2021Quote: ... coverslips were washed three times in 1x PBS for 5 min and incubated with secondary antibodies [donkey anti-rabbit Alexa594 (1:1000, #A21207, Invitrogen) and goat anti-mouse Alexa488 (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... with 5% CO2 at 37°C and propagated in the same media containing penicillin-streptomycin-glutamine (1:100 dilution of Gibco Penicillin-Streptomycin-Glutamine 100X ...
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bioRxiv - Neuroscience 2020Quote: ... recombinant human epidermal growth factor (5 ng/ml) and additionally supplemented with freshly added recombinant human fibroblast growth factor-basic (1 ng/ml; Gibco). hCMEC/D3 cells used for the experiments were between passages 6 and 11 ...
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bioRxiv - Biochemistry 2021Quote: ... 0.5 mM EDTA and 0.5 % NP40) complemented with 1 × protease inhibitors (Pierce Protease Inhibitor Mini Tablets, EDTA-Free, Thermo Scientific) and 2 U Turbo DNase (Ambion ...
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bioRxiv - Biochemistry 2020Quote: ... 5 washes of TBST were done followed by development using 1-Step Ultra TMB-ELISA Substrate Solution (ThermoFisher Scientific, 43028) and quenching with 1N HCl ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were washed three times for 5 min and incubated with 1:3000 dilution of horseradish peroxidase-conjugated Goat anti-mouse IgG Fc secondary antibody (Invitrogen) for 1 h ...
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bioRxiv - Microbiology 2021Quote: ... siRNA reverse transfection was performed by mixing 1.2 μl siRNA 5 μM with 1 μl lipofectamine RNAiMAX transfection reagent (Invitrogen, ThermoFisher Scientific) to a final volume of 100 μl with Opti-MEM® (Gibco ...
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bioRxiv - Cancer Biology 2021Quote: MDA-MB-231 cells were plated for 1 to 5 hrs on Oregon Green 488 (OR488) or AF594-conjugated cross-linked gelatin (Invitrogen) in EBSS or CM medium in the presence or absence of rapamycin as previously described 38 ...
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bioRxiv - Biophysics 2020Quote: ... for 5 min at room temperature and washed twice with phosphate buffered solution (1×, pH 7.4, Gibco, Thermo Fisher Scientific). The microchips were then coated with fibronectin (15 μg/mL ...
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bioRxiv - Biophysics 2020Quote: ... for 5 min at room temperature and washed twice with phosphate buffered solution (1×, pH 7.4, Gibco, Thermo Fisher Scientific). The microchips were then coated with fibronectin (15 μg/mL ...
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bioRxiv - Neuroscience 2020Quote: ... HA-AP2M1 and eGFP were co-transfected at a plasmid ratio of 10:10:1 into neurons at DIV 5 using Lipofectamine 2000 reagent (Invitrogen). At 48 hours post-transfection ...
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bioRxiv - Cell Biology 2021Quote: ... and suspension diluted 1/5 by adding FACS staining buffer (SB; 2% heat-inactivated Fetal bovine serum (Life Technologies; 26140079), 25 mM HEPES (Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... THP-1 was cultured in RPMI-1640 medium supplemented with 10% heat-inactivated FBS and 5% Penstrep (Invitrogen and Himedia) in an incubator fixed at 37°C and equipped with 5% CO2 supply ...
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bioRxiv - Plant Biology 2020Quote: ... anthers were stained for a week at room temperature with 1 mM Calcofluor white for imaging of cell walls and 5 μM Syto13 (ThermoFisher) for imaging nuclei ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were cross-linked for 15 minutes at room temperature with 1% formaldehyde and quenched for 5 mins at room temperature with 0.2 M glycine (Thermo Fisher). The cross-linked cells were aliquot (~ 3X106 cells ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was then diluted 1:5 in 1x Assay buffer and processed according to the kit instructions (DNA Damage Competitive ELISA Kit, Invitrogen). The initial DNA concentration used in the assay was optimized to ensure the resulting values fall within the optimum range of kit detection limits (between Std3 and Std6) ...
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bioRxiv - Developmental Biology 2022Quote: ... Blots were incubated for 5 minutes with 1 ml of Supersignal West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific, 34095) and the final blot were imaged using the ‘chemi high-resolution’ setting on a Bio-Rad ChemiDoc MP System.
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bioRxiv - Neuroscience 2021Quote: ... and these cells were cultured by a standard protocol at 37 °C with 5 % CO2 in DMEM supplemented with 10% FBS and 1% penicillin-streptomycin (ThermoFisher).
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bioRxiv - Microbiology 2021Quote: Bacteria (~5 × 107 bacteria/ml) added to RPMI-HSA supplemented with 1 μM of Sytox Green Dead Cell stain (Thermofisher) or 30 μM DiOC2 (PromoCell) ...
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bioRxiv - Developmental Biology 2021Quote: ... a mix containing a 5:1 molar ratio of the pA-uL11K3A-HA expression vector and the selection plasmid pCoBlast (Invitrogen) was prepared ...
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bioRxiv - Microbiology 2022Quote: ... siRNA reverse transfection was performed by mixing 1.2 µl siRNA 5 µM with 1 µl lipofectamine RNAiMAX transfection reagent (Invitrogen, ThermoFisher Scientific) to a final volume of 100 µl with Opti-MEM® (Gibco ...
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bioRxiv - Developmental Biology 2022Quote: ... were generated from H9 hESCs and cultured in suspension in the presence of BMP4 (1 ng/mL) and ROCK inhibitor (5 uM) for 24 hours in StemPro-34 medium (Gibco) supplemented with L-glutamine (Gibco) ...
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bioRxiv - Immunology 2022Quote: ... albicans hyphae at roughly MOI 5 were co-incubated with Sytox green (final concentration 1 μM) (Life Technologies, Eugene, OR) for 5 hours at 37°C with 5% CO2 ...
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bioRxiv - Plant Biology 2019Quote: ... and appropriate buffer containing deoxynucleoside triphosphates (dNTPs) and MgCl2 in a final concentration of 5 mM (1×; Thermo Fisher Scientific), was incubated at 25°C for 10 min and at 37°C for 30 min ...
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bioRxiv - Bioengineering 2019Quote: ... was diluted 1:200 in PBS/5% FCS/0.1% Tween® 20 and primary MMP1 antibody (mouse anti-human; Invitrogen Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by 1 h incubation with 1:1000 anti-mouse Alexa Fluor 488 conjugated secondary antibody (diluted with PBS containing 5% BSA, Invitrogen) at RT in the dark ...
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bioRxiv - Microbiology 2021Quote: ... The cells were left to fix for 1 h at room temperature before transferring 5 μl into 15 μl ProLongTM Gold Antifade mountant (ThermoFisher). For cell membrane staining ...
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bioRxiv - Molecular Biology 2021Quote: To label HELB with QDs we incubated a 1:5 molar ratio solution of biotinylated HELB and streptavidin-coated QDs 525 (Q10143, Invitrogen) for 30 min on ice ...
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bioRxiv - Microbiology 2021Quote: ... cells were washed once with PBS and incubated for 1 h at 37°C and 5% CO2 with Opti-MEM (Gibco) containing the indicated inhibitors ...
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bioRxiv - Cancer Biology 2019Quote: ... Input for each sample consisted of 500 ng of RNA and 5 μL of 1:500 diluted ERCC spike-in mix 2 (Ambion). Libraries were amplified for 11 cycles during the final amplification step ...
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bioRxiv - Biochemistry 2020Quote: ... charged by Ni2+ ions or 1 ml Tricorn 5/10 column (Cytiva) packed by Ni-NTA resin (Thermo Fisher Scientific). Columns were equilibrated by the 50 mM Na-PO4 ...
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bioRxiv - Molecular Biology 2019Quote: ... The percentage of CD4-expressing cells was measured after incubation for 10 minutes with 1 µl of anti-CD4 antibody coupled to Alexa 647 (rat isotype, RM4-5, Invitrogen). For each cell line ...
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bioRxiv - Biochemistry 2019Quote: ... blocked with 5% milk in TBST and probed with 6x-His Tag Monoclonal Antibody (HIS.H8) (ThermoFisher MA1-21315, 1:3000). Blots were incubated with primary antibodies overnight at 4 °C with rocking and were then washed (3×5min ...
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bioRxiv - Microbiology 2019Quote: Images were captured using an EVOS XL Core (Fig 1) or EVOS FL (Fig 5) Cell Imaging System (ThermoFisher Scientific). To measure transfection efficiency ...
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bioRxiv - Genetics 2020Quote: ... centrifuged for 5 minutes at 400g and resuspended in B27 medium (Neurobasal supplemented with 1% B27 without vitamin A (Gibco), 2 mM L-glutamine and 0.2% penicillin/streptomycin Y27632 (10 µM) ...
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bioRxiv - Immunology 2021Quote: ... radius and ulna dependent on the cell numbers required and collected into PBS/5% (v/v) FBS/1% (v/v) Penicillin/Streptomycin (ThermoFisher). All subsequent processing was undertaken in a biological safety level 2 tissue culture hood with sterile reagents ...
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bioRxiv - Microbiology 2021Quote: ... or hydrogen peroxide (5 and 10 mM) and incubated with or without 50 μM of diphenyl-1-pyrenylphosphine (DPPP) (ThermoFisher) at 37°C (68 ...
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bioRxiv - Cell Biology 2021Quote: ... and the suspension diluted 1/5 by adding FACS buffer (FB; 2% heat-inactivated Fetal bovine serum (Life Technologies; 26140079), 25 mM HEPES (Life Technologies ...
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bioRxiv - Physiology 2021Quote: ... Plates were incubated for 1 h at 37°C with 5% CO2 and were then overlaid with 1X MEM (Gibco) containing 2% FBS and 0.6% Avicel for 2 days ...
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bioRxiv - Biochemistry 2021Quote: ... and 300 mM NaCl] was incubated with 20-fold excess (1 mM final concentration) of tetramethylrhodamine-5-maleimide (TMR) (Invitrogen) at room temperature in the dark for 2–4 h ...
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bioRxiv - Biochemistry 2021Quote: ... Pellets were dried for 20 min at room temperature then resuspended in 5 μL of 1 M Tris and 10 μL of protein loading buffer (2x NuPAGE LDS sample buffer (Invitrogen), 715 mM 2-mercaptoethanol) ...
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bioRxiv - Genomics 2022Quote: ... THP-1 cells were separately transduced with lentivirus expressing sgRNA targeting each candidate gene and selected with 5 μg mL-1 Puromycin (A1113802, Gibco) for 7 days ...
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bioRxiv - Cell Biology 2020Quote: ... with 10% FBS and 1% pen/strep and MCF10A cells were cultured in DMEM/F-12 supplemented with 5% horse serum (Gibco), 20ng/ml epidermal growth factor ...
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bioRxiv - Cell Biology 2021Quote: Whole fly RNA was prepared from mixed sex samples (5-15 flies per sample, flies < 1 week old) with Trizol reagent (Invitrogen) according the manufacturer’s protocol ...