Labshake search
Citations for Thermo Fisher :
9051 - 9100 of 10000+ citations for 6H 1 3 Dioxolo 4 5 g 1 benzopyran 6 one 7 8 dihydro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2023Quote: ... tissues were washed and stained for 48 h at 4°C with a 1:100 dilution of goat anti-mouse secondary antibody conjugated to Alexa-Fluor 488 (A28175, Life Technologies), goat anti-rabbit secondary antibody conjugated to Alexa-Fluor 555 (A21428 ...
-
bioRxiv - Cell Biology 2023Quote: Human nasal brushings were collected fresh for this study and immediately placed in a 15-ml sterile Falcon tube containing 4 ml of transport medium (αMEM supplemented with 1× penicillin–streptomycin (Gibco, 15070), 10 ng/ml gentamicin (Gibco ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... The following day embryos were thoroughly washed with NPBS + 0.1% Triton and incubated first in blocking solution for 3 hours and then overnight at 4°C in the dark in blocking solution with DAPI (1:500) and secondary antibodies: goat anti-mouse (Invitrogen 84540) and goat anti-rabbit (Abcam 150083) ...
-
bioRxiv - Developmental Biology 2023Quote: ... Sections were incubated with primary antibody overnight at 4°C and then incubated with Alexa Fluor-conjugated donkey secondary antibodies (1:400, Molecular Probes) at RT for 2 hrs and then mounted in VECTASHIELD medium with 4′-6-Diamidino-2-phenylindole (DAPI ...
-
bioRxiv - Molecular Biology 2023Quote: ... was equilibrated with lysis buffer and then incubated with the cell lysate at 4 °C for 1 h on an end-to-end rotator (Cat# 88881001, ThermoFisher scientific). The slurry was transferred to a 15 ml falcon tube ...
-
bioRxiv - Immunology 2023Quote: Splenic B cells were resuspended at a concentration of 5 × 106 cell/ml in RPMI supplemented with 20 mM HEPES and 2.5% FCS and loaded with 1 μM Fluo-4 (Thermo Fisher, #F14201) and 3 μM Fura Red (Thermo Fisher ...
-
bioRxiv - Cancer Biology 2023Quote: ... were mixed in a 3:1 with sample buffer (NuPAGE™ LDS Sample Buffer (4X) + 2-Mercaptoethanol in 2:1) and loaded onto a precast Gel (NuPAGE™ 4-12% Bis-Tris Gel, Invitrogen). Proteins were transferred to nitrocellulose membranes ...
-
bioRxiv - Microbiology 2023Quote: ... Midguts were incubated with primary antibody anti-flavivirus envelope 4G2 antibody produced in-house in 1X PBS at 4 °C overnight followed by 1:500 secondary antibody anti-mouse IgG Alexa 488 (Invitrogen, A28175) in 1X PBS and Hoechst33342 (Invitrogen ...
-
bioRxiv - Genetics 2023Quote: ... and 4 µL (<1 µg) of DNase treated total RNA was then reverse transcribed into cDNA (Superscript III Supermix, Thermo Fisher) according to manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2023Quote: ... The cells were then incubated for 30 min at 4 °C with AF647-conjugated goat anti-mouse IgG (1:500, Thermo Fisher). Cholesterol levels were determined by incubation for 30 min at 4 °C with filipin (50 µg/mL) ...
-
bioRxiv - Developmental Biology 2023Quote: ... for one hour at RT and stained overnight at 4°C with the primary antibody rabbit anti-GFP diluted 1:500 (#A11122 Thermo Fisher). Samples were washed three times using 0.3% PBT and stained with secondary antibody Alexa Fluor 488 Goat anti-Rabbit diluted 1:1000 (#R37116 ...
-
bioRxiv - Cancer Biology 2023Quote: ... Membranes were incubated overnight at 4°C with the following antibodies: mouse anti-Beta-actin (1:1000, Thermo Fisher Scientific, AM4302) and rabbit anti-PROX1 (1:1000 ...
-
bioRxiv - Microbiology 2023Quote: ... the membrane was incubated O/N at 4°C with gentle agitation with 1:2000 goat anti-human complement C3 antibody (Thermo Scientific) diluted in 1X TBS/0,5% Tween/3% BSA ...
-
bioRxiv - Neuroscience 2023Quote: ... then incubated for 2 hours at 4°C in a 1:1000 solution of AF-568 donkey anti-mouse secondary antibody (Invitrogen #A10037) in blocking solution ...
-
bioRxiv - Developmental Biology 2023Quote: ... and then incubated ON at 4°C with the following primary antibodies in blocking solution: chicken anti-GFP (Invitrogen, 1:1000), rabbit anti-somatostatin (BMA-biomedials ...
-
bioRxiv - Immunology 2023Quote: ... The samples were then stained with the blood lineage antibody panel (Table 1) for 30 minutes at 4°C and then washed with PBS (ThermoFisher Scientific) and 2% (v/v ...
-
bioRxiv - Immunology 2023Quote: ... was incubated overnight at 4°C and Alexa Fluor 488 goat anti-chicken IgY (H+L) secondary antibody (1:100, A-11039, ThermoFisher Scientific) was applied to detect GFP+ cells ...
-
bioRxiv - Neuroscience 2023Quote: ... 30 µg of TDP-43 protein extract or 50µg of RGNEF extract was then resuspended in 1 X NuPAGE LDS Sample buffer (4 X) (Thermo Fisher Scientific) and denaturated at 85°C for 5 min ...
-
bioRxiv - Molecular Biology 2023Quote: ... The cells were then incubated overnight at 4°C with a 1:100 dilution of leptin receptor antibody (cat#PA1-053, ThermoFisher Scientific) in blocking buffer ...
-
bioRxiv - Genetics 2023Quote: ... Cells were incubated with primary antibodies overnight at 4 °C and probed with the appropriate secondary antibodies for 1 hour at room temperature (ThermoFisher Scientific). Primary and secondary antibodies were resuspended in 0.2% Gelatin in PBS ...
-
bioRxiv - Molecular Biology 2023Quote: ... plates were washed (4 x 300 µL PBST) and primary antibodies were added in 1 : 10000 dilution (anti-IgG Fc cat. 31789, Thermo Fisher; anti-His-Tag cat ...
-
Mitochondrial Apolipoprotein MIC26 is a metabolic rheostat regulating central cellular fuel pathwaysbioRxiv - Cell Biology 2023Quote: ... washed three times with TBS-T and probed at 4°C overnight with the following primary antibodies: MIC26 (Invitrogen, 1:1000), MIC27 (Sigma-Aldrich ...
-
bioRxiv - Bioengineering 2023Quote: ... samples were incubated overnight at 4 °C with secondary antibody Alexa 647 donkey-α-rabbit (1:500 in blocking solution; Invitrogen), Alexa 568 donkey-α-mouse (1:500 in blocking solution ...
-
bioRxiv - Neuroscience 2023Quote: ... Slides were washed with PBS 15 mins × 4 in a slide mailer and incubated in secondary antibody at 1:1000 dilution (goat anti-chicken 647, Themo Fisher Scientific Cat# A-21449 and goat anti-rabbit 546 ...
-
bioRxiv - Neuroscience 2023Quote: ... Cells were then washed with PBS with 0.03% NaN3 for 15 mins RT × 4 and incubated in secondary antibodies at 1:1000 dilution (goat anti-mouse 488, Thermo Fisher Scientific# A-28175 ...
-
bioRxiv - Molecular Biology 2024Quote: ... lysed and sonicated using Diagenode Bioruptor® Pico sonicator for 10 cycles with alternating 30 secs on/ off at low intensity and 1 mg of protein was digested with 4 U of Turbo DNase (Ambion, AM2238) and 0.28 U/ml (low ...
-
bioRxiv - Neuroscience 2024Quote: ... Then the sections were incubated with primary antibodies in blocking buffer overnight at 4°C: AT8 (Mouse, 1:100, Thermo Scientific), and Iba1 (Rabbit ...
-
bioRxiv - Cancer Biology 2024Quote: ... A172 cell line was cultured in high glucose DMEM media plus 4 mM L-glutamate and 1 mM sodium pyruvate (Thermo Fisher) supplemented with 10% FBS and 1% penicillin and streptomycin mixture ...
-
bioRxiv - Neuroscience 2024Quote: ... Adult flies were fixed with 4% paraformaldehyde in 1X PBS for 1 hour after a quick dissection,then immersed in OCT compound (Fisher Scientific) and flash frozen in liquid nitrogen or dry ice ...
-
bioRxiv - Immunology 2024Quote: ... the slices were incubated for 24 hours at 4°C with Alexa Fluor 488 secondary antibody (1:750, Thermo Fisher Scientific). Finally ...
-
bioRxiv - Biochemistry 2024Quote: A total of 1 μg of plasmid DNA (TLR3:minibinder-protein A ratio of 1:4) was transfected into 2.5×106 Expi 293F cells (#A14527, Thermo Fisher Scientific) using Expifectamine (#A14524 ...
-
bioRxiv - Biochemistry 2024Quote: ... Excess sample was removed with a blotting time of 4 s and a blotting force of 1 at 4 °C prior to plunge freezing into liquid ethane using a Vitrobot Mark IV (Thermo Fisher). A total of 8,294 movies were recorded with a K3 detector (Gatan ...
-
bioRxiv - Biochemistry 2024Quote: ... Excess sample was removed with a blotting time of 4 s and a blotting force of 1 at 4 °C prior to plunge freezing into liquid ethane using a Vitrobot Mark IV (Thermo Fisher). A total of 9,018 movies were recorded with a K3 detector (Gatan ...
-
bioRxiv - Neuroscience 2024Quote: ... Brains were then washed and incubated overnight at 4°C with secondary antibodies Goat-anti-Chicken 488 (1:400, Thermo Fisher Scientific Cat# A-11039 ...
-
bioRxiv - Developmental Biology 2024Quote: ... The fixed ovaries were washed in washing buffer (WB, 1% BSA in 1X PBS) thrice before being embedded into 4% low-melting point agarose (Thermo Fisher). The embedded tissue was sliced into 100 µm thick tissue sections using a vibratome (Leica ...
-
bioRxiv - Developmental Biology 2024Quote: ... for one hour at RT and stained overnight at 4 °C with primary antibody Rabbit anti-GFP diluted 1:500 (#A11122 Thermo Fisher). Samples were washed three times using 0.3% PBT and stained with secondary antibody Alexa Fluor 488 Goat anti-Rabbit diluted 1:1000 (#R37116 ...
-
bioRxiv - Immunology 2024Quote: ... CAL-1 cells were infected with Salmonella for 4 h and then incubated for 30 min at 37 °C with CellROX Deep Red Reagent (1 µM, #C10422 Invitrogen Thermofisher) for total (cytoplasmic and nuclear ...
-
bioRxiv - Molecular Biology 2024Quote: ... Fractions 1-4 were pooled and concentrated using centrifugal filters units (30 kDa MWCO, MilliporeSigma Amicon Ultra Centrifugal Filter Unit, Fisher Scientific) at 4000 × g for 30 min to a final volume of 100uL.
-
bioRxiv - Cell Biology 2024Quote: ... Part of the supernatant was conserved as total lysate and the remainder was subjected first to a preclearing step with agarose for 1 h at 4°C and then to pull-down assay with NeutrAvidin agarose (Thermo Scientific) for 2 h at 4°C to isolate biotinylated proteins.
-
bioRxiv - Neuroscience 2024Quote: ... Neurobiotin-injected specimens for the tracer-coupling analysis of HCs were first incubated overnight at 4°C with Alexa568-conjugated streptavidin (1:250, Invitrogen, S11226), diluted in incubation buffer containing 10% NDS ...
-
bioRxiv - Pathology 2024Quote: ... pH 7.4 and 500 mM NaCl, 20 mM imidazole, 1 mM PMSF, protease inhibitor cocktail Set III, EDTA free from Thermo Fisher) and lysed by an LM20 microfluidizer (15000 psi) ...
-
bioRxiv - Cell Biology 2024Quote: ... overnight at 4°C followed by incubation for 1 hour with secondary goat anti-mouse IRDye 800 (Thermo Fisher Scientific, MA) or goat anti–rabbit Alexa Fluor 680 (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2024Quote: ... the cells were incubated overnight at 4°C with an anti-HIV-1 p24 monoclonal (AG3.0) primary antibody (4 µl/mL) (NIH HIV Reagent Program catalog# 4121) or P2RY12 primary antibody (1:100, 702516, Thermo Fisher) or TMEM119 primary antibody (1:50 ...
-
bioRxiv - Biochemistry 2024Quote: ... Excess sample was removed with a blotting time of 4 s and a blotting force of 1 at 4 °C prior to plunge freezing into liquid ethane using a Vitrobot Mark IV (Thermo Fisher). A total of 6,812 movies were recorded with a K3 detector (Gatan ...
-
bioRxiv - Biochemistry 2024Quote: ... Excess sample was removed with a blotting time of 4 s and a blotting force of 1 at 4 °C prior to plunge freezing into liquid ethane using a Vitrobot Mark IV (Thermo Fisher). A total of 6,650 movies were recorded with a K3 detector (Gatan ...
-
bioRxiv - Cell Biology 2020Quote: ... on a Step One Plus Real-Time PCR thermal cycler (Step One software v2.2.2; Applied Biosystems, Thermo Fisher Scientific). The Taqman gene expression assays for the reference gene (GAPDH – ID# Hs02786624_g1 ...
-
bioRxiv - Cell Biology 2020Quote: ... on a Step One Plus Real-Time PCR thermal cycler (Step One software v2.2.2; Applied Biosystems, Thermo Fisher Scientific). The Taqman gene expression assays for the reference gene (GAPDH – ID# Hs02786624_g1 ...
-
bioRxiv - Cell Biology 2022Quote: ... with a one-step RT-PCR kit using Taqman® technology (AgPath-IDTM One-Step RT-PCR, Life Technologies) and using the Applied Biosystems ViiA 7 Real-Time PCR System and the appropriate primers for Taqman assays (Life Technologies ...
-
bioRxiv - Neuroscience 2024Quote: ... To determine RNA purity and concentration we used the NanoDrop One/OneC spectrophotometer (ThermoFisher, Cat. No. ND-ONE-W). Then ...
-
bioRxiv - Bioengineering 2020Quote: ... penicillin (100 μ g/ml) and streptomycin (100 μ g/ml) (Gibco-BRL), and maintained in humidified atmosphere of 5% CO2 at 37ºC.