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Citations for Thermo Fisher :
8551 - 8600 of 10000+ citations for 5 NITROFURFURYL ALCOHOL since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... 5% NP-40% [Tergitol]) supplemented with 1X Halt protease and phosphatase inhibitor cocktail (from Thermo Scientific, Rockfold, IL, USA) and 1mM phenylmethylsulfonyl fluoride (PMSF) ...
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bioRxiv - Microbiology 2021Quote: MDCK cells were maintained in Eagle’s minimal essential medium (EMEM) containing 5% fetal bovine serum (Gibco, Cergy Pontois, France), 100 U/ml penicillin and 100 μg/ml streptomycin (Hyclone ...
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bioRxiv - Cancer Biology 2020Quote: ... the cell membrane was stained with 5-Hexadecanoylaminofluorescein and the nucleus with and DAPI (Thermo Fisher Scientific, Waltham, MA). The cells were fixed in 3% formaldehyde and permeabilized using 0.1% Triton-100 buffer ...
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bioRxiv - Neuroscience 2021Quote: ... The substrates were placed in an incubator (37 °C, 5 % CO2, Steri-Cycle 371 CO2 Incubator, Thermo Fisher Scientific) until seeding.
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bioRxiv - Neuroscience 2021Quote: ... slides were washed in 3×5’ PBS and incubated in goat-α-rabbit-555 (1:1000, Life Technologies, A21207) in PBS for 90’ ...
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bioRxiv - Neuroscience 2020Quote: ... the sections were washed in 0.1 M TB (3 ×5 minutes) and then incubated in goat anti-chicken Alexa 488 (1:1000, #A11039, Invitrogen), goat anti-rabbit Alexa 568 (1:500 to 1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... The culture medium was changed every 2 to 3 days and the cells were split every 5 to 7 days using 0.25% Trypsin-EDTA (Gibco), up to 20 times ...
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bioRxiv - Biophysics 2021Quote: ... cell nuclei were stained by exposure for 5 min to 1 mg/100 ml Hoechst 33342 (Thermo Fisher Scientific) in water.
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bioRxiv - Immunology 2021Quote: ... Cells were co-cultured at 37°C with 5% CO2 during 21 or 25 days in RPMI-1640 (Invitrogen) supplemented with 10% HyClone FBS (Thermo Scientific) ...
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bioRxiv - Biochemistry 2021Quote: ... and an Acclaim PepMap 100 trap column (100 μm × 2 cm, nanoViper, C18, 5 μm, 100 Å; Thermo Scientific) were used to separate tryptic peptides by increasing concentrations of 80 % (v/v ...
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bioRxiv - Microbiology 2021Quote: ... and an Acclaim PepMap 100 trap column (100 μm x 2 cm, nanoViper, C18, 5 μm, 100Å; Thermo Scientific), the tryptic peptides were separated by increasing concentrations of 80% acetonitrile / 0.1% formic acid at a flow of 250 nL/min for 120 minutes and analyzed with a QExactive Plus mass spectrometer (Thermo Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Samples of 5 μL were loaded onto a trapping column (Acclaim PepMap100 C18, 75 μm × 2 cm, Thermo Scientific) using 96% eluent A (0.1% formic acid ...
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bioRxiv - Immunology 2021Quote: ... were heated to 95°C for 5 min and 10 μl (2.5 μg) were loaded to a NuPAGE 10% Bis-Tris gel (Invitrogen). The gel was run in NuPAGE MOPS Buffer (Invitrogen ...
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bioRxiv - Bioengineering 2021Quote: ... The slides were counterstained with DAPI (1:5,000) for 5 min and mounted with Prolong Diamond Antifade Mountant (Invitrogen). A Nikon A1 laser-scanning confocal microscope was used to acquire confocal images ...
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bioRxiv - Molecular Biology 2022Quote: ... 5 µg of this total RNA input was loaded onto a precast 10% TBU acrylamide urea gel (Novex / ThermoFisher) and electrophoresed at 120 V for 15 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... The transfer conditions used were a 0.45-μm polyvinylidene fluoride (PVDF) membrane transferred in 5% Transfer Buffer (Invitrogen, NP00061) at 20 mA for 840 min ...
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bioRxiv - Microbiology 2022Quote: ... and loaded onto a 5 mL GE Healthcare HisTrap™ FF Crude prepacked column (Thermo Fisher Scientific; Cat.# 11723219). Proteins were eluted by FPLC using an ÄKTA purifier (Amersham BioScience ...
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bioRxiv - Molecular Biology 2022Quote: ... Tryptic peptides were loaded onto a trap column (Pepmap 100 5μm, 5 × 0.3 mm, ThermoFisher Scientific, San Jose, CA) at a flow rate of 10 μL/min using 0.1% TFA as loading buffer ...
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bioRxiv - Microbiology 2022Quote: ... with the only difference that a 22 x 5 mm strip of PVC coverslips (ref 12-547, Fisher Scientific) placed vertically in the microtube before sealing with Aeraseal ...
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bioRxiv - Microbiology 2022Quote: Reverse transcription of total RNA was performed on selected samples with 5 μg of RNA and 12.5 ng/μl of random hexamer primer (Invitrogen) using SuperScript™ III reverse transcriptase (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... 20 μl resuspended parasite culture was incubated with dihydroethidium (5 μg/ml, Cayman) and SYBR Green I dye (0.25 x dilution, Invitrogen) in a final volume of 100 μl medium for 20 min at RT protected from light ...
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bioRxiv - Microbiology 2022Quote: ... were grown in suspension culture in Joklik’s minimum essential medium (JMEM) (US Biological) supplemented to contain 5% fetal bovine serum (FBS) (Gibco) and 2 mM L-glutamine ...
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bioRxiv - Pathology 2022Quote: ... resulting from UA were determined via a membrane-permeant JC-1 dye (5, 50, 6, 60 -tetrachloro-1, 10,3,30 -tetraethylbenzimidazol-carbocyanine iodide, Molecular Probes). JC-1 dye accumulates in mitochondria in a potential-dependent manner ...
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bioRxiv - Neuroscience 2022Quote: ... and the pellet was resuspended in 5 mL B-PER™ complete bacterial protein extraction reagent (Thermo Scientific, #89821) supplemented with 2 mM DTT ...
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bioRxiv - Biochemistry 2022Quote: ... Nuclear translocation of AR signal was validated by staining LNCaP cells grown in RPMI-1640 5% CSS (Gibco, A3382101) with the same protocol ...
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bioRxiv - Immunology 2022Quote: Vaccine 5: The above mentioned hCMP-mRBD antigen when expressed from Stable CHO (Thermo Fisher Scientific, Cat no. R75807) cell lines was named as hCMP-mRBD-CHO ...
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bioRxiv - Cell Biology 2022Quote: ... fixed fat bodies were washed twice for 5 min in PBS and then were incubated with 0.165 μM Alexa Fluor 488 phalloidin (Invitrogen) in PBSTG for 30 min at RT ...
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bioRxiv - Cancer Biology 2022Quote: ... 2 μg of each vector were used to transfect 5×105 MC38 cell line with Lipofectamine 2000 (ThermoFisher Scientific) in Opti-MEM medium (ThermoFisher Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: ... Red blood cells were removed by treatment with 3-5 mL of ACK lysis buffer (Gibco, Cat. No. A1049201) and a subsequent washing step in RPMI 1640 with 10% FBS ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were washed in lysis buffer 5 times and elutes in loading buffer (NuPAGE LDS sample buffer, Thermo Fisher, Cat ...
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bioRxiv - Biochemistry 2022Quote: Cells were dissociated for 5 minutes with either trypsin (0.5% v/v) or an enzyme-free cell dissociation buffer (CDB) (Thermofisher). Although the exact composition is not disclosed by Thermofisher ...
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bioRxiv - Immunology 2022Quote: Total RNA was extracted from 5 adult flies collected at different time points after Toll activation with TRIzol (Invitrogen). cDNA was synthesized from 1000 ng total RNA using the SuperScript II Reverse Transcriptase kit (Transgene) ...
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bioRxiv - Cancer Biology 2022Quote: ... MCF7 and T47D cells were cultured at 37°C with 5% CO2 in DMEM (Thermo Fisher Scientific, Gibco 41966029) supplemented with 9% FBS (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... MCF7 and T47D cells were cultured at 37°C with 5% CO2 in DMEM (Thermo Fisher Scientific, Gibco 41966029) supplemented with 9% FBS (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2022Quote: ... and maintained at 37°C and 5% CO2 in 175 cm2 culture flasks using high glucose DMEM (Gibco, ThermoFisher), supplemented with 10% v/v fetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2022Quote: ... and maintained at 37°C and 5% CO2 in 175 cm2 culture flasks using high glucose DMEM (Gibco, ThermoFisher), supplemented with 10% v/v fetal bovine serum (FBS ...
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bioRxiv - Biophysics 2022Quote: ... 5 μl red, green and orange fluorescent beads (of indicated diameter, 20 nm, 100 nm, 200 nm) (Fluospheres, Invitrogen) were added to the mix (from a 1:1000 dilution in water) ...
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bioRxiv - Pathology 2020Quote: ... CHO cells were transfected with 5 ug of human cadherin-6 in pIRES-puro or mouse cadherin-6 in pCDNA3.1 via Lipofectamine 2000 (Invitrogen). After 48hrs ...
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bioRxiv - Biochemistry 2019Quote: HAP1 cells were grown at 37°C with 5% CO2 in Iscove’s Modified Dulbecco’s medium (IMDM) (Gibco, cat.# sh30228) supplemented with 10% (v/v ...
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bioRxiv - Bioengineering 2019Quote: ... Detachment of confluent cells was achieved by a 5 minutes incubation in 0,05% Trypsin-EDTA (Trypsin-EDTA 0.25%, 25200-072, Gibco®). Cells were re-suspended and counted using 0.4% Trypan Blue (T8154-20ML ...
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bioRxiv - Neuroscience 2019Quote: ... Cell cultures were maintained in an incubator with 5% CO2 (Forma Series II Water Jacket CO2 Incubator, Thermo Scientific) at 37 °C ...
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bioRxiv - Molecular Biology 2019Quote: ... were maintained in monolayer culture at 37°C and 5% CO2 in one of the following medium: Medium A contained DMEM (4.5 g/L glucose; Gibco) supplemented 100 U/ml penicillin and 100 µg/ml streptomycin sulfate ...
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bioRxiv - Cancer Biology 2019Quote: ... T47D:C4217 cells were grown in 5% charcoal stripped calf serum in phenol red free modified RPMI media (Life Technologies). These cells represent acquired estrogen independence and endocrine therapy resistance in estrogen receptor positive breast cancer ...
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bioRxiv - Cell Biology 2019Quote: ... were grown for 24h on coverslips before being incubated for 30 min with 100µM of 5-ethynyl uridine (EU from ThermoFisher). After washes with PBS ...
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bioRxiv - Microbiology 2019Quote: ... and glycerine (STGG) medium and cultured on Columbia blood agar supplemented with 5% horse blood (PB0122A, Oxoid/Thermo Scientific) and 80μl gentamycin 1mg/mL (G1264-250mg ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were passaged two times a week 1:5 in culture-treated flasks using TrypLE dissociation media (ThermoFisher 12604039).
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bioRxiv - Cell Biology 2019Quote: ... 5 mg protein was incubated with 125 μg α-GFP antibody covalently coupled to M270 magnetic resin (ThermoFisher Scientific) for 1 h with rotation ...
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bioRxiv - Developmental Biology 2019Quote: ... 1:200 dilution for 5 minutes prior to plating and BA2 tissue was soaked in DiI (V22889, Thermofisher Scientific), 1:30 dilution for 30 minutes ...
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bioRxiv - Molecular Biology 2019Quote: ... Enzymes were inactivated by adding 5 ml DMEM/F-12 + 10% HyClone fetal bovine serum (Catalog #SH3091003, Fisher Scientific). Dissociated primary cells were spun down at 1000 rpm for 5 minutes ...
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bioRxiv - Physiology 2019Quote: ... 10 μm thickness muscle cryosections were incubated with 5 μM of 2’,7’-dichlorodihydrofluorescein diacetate (DCFH; Molecular Probes, Eugene) and allowed to dry overnight at room temperature in dark ...