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Citations for Thermo Fisher :
8751 - 8800 of 10000+ citations for 5 NITROFURFURYL ALCOHOL since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Remaining cells were centrifuged for 5 minutes at 500 g before 100µL of extraction buffer added (Arcturus Picopure RNA kit, Thermofisher) and lysates frozen at −80°C.
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bioRxiv - Microbiology 2021Quote: ... cell medium was replaced by fresh medium supplemented with 5 µM Hoechst 33342 nucleic acid stain (Thermo Fisher Scientific) as a background stain for high-content imaging analysis ...
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bioRxiv - Microbiology 2021Quote: ... 5 uL of re-suspended BAC16 DNA was incubated with 100 uL Opti-MEM reduced serum media (Thermo Fisher) for 5 minutes ...
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bioRxiv - Microbiology 2020Quote: ... and EFKB3 cells were cultured in Dulbecco’s minimal essential medium (DMEM) supplemented with heat inactivated fetal bovine serum(5 or 10%) and antibiotic/antimyotic (GIBCO). Both NP an OP swabs were used for virus isolation ...
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bioRxiv - Microbiology 2021Quote: ... 5 ml of culture were grown in 20 mm × 150 mm glass test tubes (Fisher Scientific, Hampton, NH, USA) with disposable ...
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bioRxiv - Developmental Biology 2021Quote: ... resuspendend in 1 mL M9 before 5 µL of DiI stock solution (2 mg/mL DiI (Molecular Probes, #D282) in dimethyl formamide ...
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bioRxiv - Cell Biology 2021Quote: ... a 6 cm dish of E14 cells 50% confluent was transfected using 2μg of pX459-Cas9-sgRNA and 5 μg of targeting construct using Lipofectamine 2000 (ThermoFisher) according to manufacturer’s guidelines ...
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bioRxiv - Genetics 2019Quote: ... 5% CO2) to 70-80% confluence in dulbecco’s modified eagle medium (DMEM) supplemented with 10% fetal bovine serum (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Genomics 2021Quote: ... Treated sections were then mounted with hybridization buffer (5%/15% Formamide; 10% Dextran sulfate Sigma D8906; 0.02% BSA Ambion AM2616 ...
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bioRxiv - Genetics 2019Quote: ... 10% β-mercaptoethanol at 95°C for 5 min and run on NuPage 10% Bis-Tris gels (Life Technologies) in 2-(N-morpholino ...
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bioRxiv - Genomics 2021Quote: ... 0.5μM oligo-dT (IDT; 100uM 5′-biotin-ACGAGCATCAGCAGCATACGA-T30VN-3′) and 0.5mM dNTPs/each (Thermo Fisher; 25 mM each) and snap frozen at −80 °C ...
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bioRxiv - Genomics 2021Quote: ... The presence of SARS-CoV-2 was determined using the 2019-nCoV CDC assay (https://www.fda.gov/media/134922/download) on the QuantStudio 5 (Applied Biosystems) (n=7) ...
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bioRxiv - Genomics 2019Quote: ... Cells were cultured at 37°C with 5% CO2 in Roswell Park Memorial Institute (RPMI) medium (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Genomics 2019Quote: ... Cells were cultured at 37°C with 5% CO2 in Ham’s F-12 Nutrient Mixture medium (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Genetics 2020Quote: ... urartu was labelled by nick translation with ChromaTide™ Alexa Fluor™ 488-5-dUTP (Invitrogen; C11397; coloured green), 2 ...
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bioRxiv - Genetics 2020Quote: ... After 5 min incubation with the developing reagent was added (Pierce® ECL Western Blotting Substrate, 32106, Thermo Scientific) and images were captured using Azure 300 imaging system (Azure Biosystems).
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bioRxiv - Cancer Biology 2021Quote: ... and an Acclaim PepMap 100 trap column (100 µm x 2 cm, nanoViper, C18, 5 µm, 100Å; Thermo Scientific), the tryptic peptides were separated by increasing concentrations of 80% ACN/0.1% formic acid at a flow of 250 nL/min for 90 min and analyzed with a QExactive HF mass spectrometer (Thermo Scientific) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The full structure of the NUKU2 transcript was determined with 5’ and 3’ RACE using the GeneRacer kit (Invitrogen), and testicle total RNA (Ambion ...
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bioRxiv - Microbiology 2020Quote: Spinner-adapted L929 cells were grown in suspension culture in Joklik’s minimum essential medium (JMEM; US Biological) supplemented to contain 5% fetal bovine serum (FBS) (Gibco), and 2 mM L-glutamine (Corning) ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were seeded at densities of 3 × 104 or 5 × 104 per 60 mm plate with a bottom layer of 0.6% Noble agar in MEM (Gibco) and a top layer of 0.5% methylcellulose containing MEGM (Lonza) ...
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bioRxiv - Microbiology 2022Quote: MDCK cells were seeded in 96-well microplates at a density of 5×103 cells/well in 100 µl Gibco Dulbecco’s Modified Eagle Medium (DMEM) (Gibco® ...
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bioRxiv - Developmental Biology 2022Quote: ... complexes were formed by mixing the sgRNA (2.5 ng/uL) with Cas9 protein (IDT, 5 ng/uL) in Opti-MEM (ThermoFisher) and incubating at 37C for 10 minutes ...
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bioRxiv - Immunology 2022Quote: ... PBMCs were cultured at 37°C with 5% CO2 for 3 days in RPMI-1640 medium (Thermo Fisher Scientific) supplemented 10% FCS (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... SK-MEL-28 and SK-MEL-5 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Life Technologies Corporation) with 10% fetal bovine serum and 1% penicillin/streptomycin ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were cultured in a humidified incubator at 37°C under 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM, Gibco) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were transfected with 5 µg of pCS2P+dkk1GFP plasmid in using Lipofectamine 2000 (Thermo Fisher Scientific, MA, USA). Dkk1GFP CM were collected on the 2nd ...
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bioRxiv - Developmental Biology 2022Quote: ... In brief: Cells were maintained in a humidified incubator at 5% CO2 and 37 °C (Thermo Fisher Scientific, USA).
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bioRxiv - Developmental Biology 2022Quote: ... the samples were washed 3x for 5 minutes in PBT and incubated in corresponding Alexa Fluor (1:500, Invitrogen) secondary antibodies for 2 hours ...
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bioRxiv - Pathology 2022Quote: ... as above and left to attach for 2 hours before adding bromodeoxyuridine / 5-bromo-2’-deoxyuridine (BrDU, Invitrogen B23151) to a final concentration of 75 µM and left to proliferate for 6 hours ...
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bioRxiv - Cell Biology 2022Quote: ... coverslips were transferred to a 2 mL imaging chamber and stained with 5 μM Fura-2AM (Molecular Probes, F1221) for 30-minutes at 37°C in complete RPMI ...
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bioRxiv - Neuroscience 2022Quote: ... 5-ethynyl-2’-deoxyuridine (EdU) was incubated using Click-iT™ Plus EdU Cell Proliferation Kit (Thermo Fisher Scientific) after DAPI staining ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were cultured at 37°C with 5% CO2 in 4.5 g/L glucose DMEM (ThermoFisher, Waltham, MA, #10566016) supplemented with 10% Fetal Bovine Serum (HyClone ...
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bioRxiv - Microbiology 2022Quote: ... 250 ng of each of the RNAs was transfected into 5 x 104 cells using Lipofectamine 2000 (Life Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: Fresh tissue samples were washed 2–3 times in PBS and incubated in 5 U/ml dispase (ThermoFisher Scientific) supplemented with antibiotics (penicillin 50U/I and streptomycin 50 mg/ml ...
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bioRxiv - Biochemistry 2022Quote: ... The remainder of the lysate was added to 5 µL of Pierce Protein G agarose beads (Thermo Fisher Scientific), and the mixture was rotated 1-2 hours at 4°C to pre-clear ...
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bioRxiv - Biochemistry 2022Quote: ... HEKT cells were grown in a humidified 37°C incubator with 5% CO2 using media supplemented with 100 I.U./mL penicillin and 100mg/mL streptomycin (Invitrogen). The human cell lines HEK293T were maintained in DMEM (VWR ...
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bioRxiv - Bioengineering 2022Quote: ... the pellet was air dried for 5 minutes prior to resuspension in nuclease-free TE buffer (Thermo Scientific #J75793).
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bioRxiv - Cell Biology 2022Quote: All HCT116 cell lines were cultured in humidified incubators at 37 °C and 5% CO2 using McCoy’s modified 5A medium (Gibco) supplemented with 10% fetal bovine serum (Gibco ...
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bioRxiv - Cell Biology 2022Quote: ... A 300 μL solution of 5% formic acid and 0.2% trifluoroacetic acid (TFA) R2 50 μm Poros (Applied Biosystems) beads slurry in water was added to the gel pieces before returning the samples to the shaker for an additional three hours at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... cells were maintained in a humidified 37° C incubator with 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM, MilliporeSigma) supplemented with 2 mM L-Glutamine (L-Glu, Gibco), 0.1 mM non-essential amino acids (NEAA ...
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bioRxiv - Cancer Biology 2022Quote: ... for 15 minutes at room temperature and then washed 3 times for 5 minutes each with pH 7.4 Phosphate Buffered Saline (PBS) (Gibco). Cells were then simultaneously permeabilized and blocked with a solution of 0.25% Surfact-Amps X-100 (Thermo Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... were diluted 1:5000 in 5 % BSA in TBST or in blocking buffer (Cat. no. 37538, Thermo Fisher Scientific) in PBS (1:1 ...
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bioRxiv - Biochemistry 2022Quote: ... Fluorescence measurements were attained by running the assay on the QuantStudio 5 real-time PCR system (Applied Biosystems™) at increasing temperatures from 25 to 95 °C at 0.1 °C s−1 increments ...
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bioRxiv - Biochemistry 2022Quote: ... 4,4-difluoro-5-(2-thienyl)-4-bora-3a,4a-diaza-s-indacene-3-dodecanoic acid (BODIPY-C12, Invitrogen D3835), was dissolved in 100% ethanol and conjugated to 10% bovine serum albumin ...
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bioRxiv - Bioengineering 2022Quote: ... the EBs were transferred to StemPro-34 with rhVEGF (5□ng/ml) for another five days and then to DMEM high glucose (4.5□g/l, ThermoFisher) media with compaction factors (CHIR (1□uM ...
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bioRxiv - Cell Biology 2022Quote: ... cells were permeabilized with saponin (0.2%) for 5 min and incubated with DAPI (1 μg/mL) (ThermoFisher, catalog #: D1306) for 3 min to stain the nucleus ...
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bioRxiv - Developmental Biology 2022Quote: HeLa cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F12) with Glutmax and 5% FCS (Gibco) and passaged every few days ...
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bioRxiv - Cell Biology 2022Quote: ... then resuspended in 100 μL staining buffer (10 mM HEPES, 140 mM NaCl, 2.5 mM CaCl2 pH 7.4, 5% Annexin-FITC (Invitrogen, A13199), 50 μg/mL propidium iodide (P1304MP ...
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bioRxiv - Developmental Biology 2022Quote: ... and 5 μl of the RT reaction was amplified in 25 μl using AccuPrime Pfx DNA polymerase (Thermo Fisher, 12344024 ...
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bioRxiv - Cell Biology 2022Quote: All cell lines were cultured at 37°C in 5% CO2 atmosphere in Dulbeccós modified medium (DMEM, Gibco TM, Thermofisher) containing 10% fetal bovine serum (FBS ...