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Citations for Thermo Fisher :
8451 - 8500 of 10000+ citations for 5 NITROFURFURYL ALCOHOL since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... The siRNAs at a concentration of 5 nM were reverse transfected using Lipofectamine RNAiMAX transfection reagent (Invitrogen, VIC, Australia) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Cells described above were cultured at 37C at 5% CO2 in Advanced Dulbecco’s Modified Eagle Medium: Nutrient mixture F-12 (DMEM-F12) with Glutamax (GIBCO), supplemented with 12% FCS (Clontech) ...
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bioRxiv - Cancer Biology 2020Quote: ... Ming-Chih Lai) (50 ng) or mutant MT-STAT1-5’UTR-Luciferase (50 ng) using Lipofectamine 3000 (Life Technologies). In HepAD38 cells ...
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bioRxiv - Cancer Biology 2020Quote: ... India were maintained in a CO2 incubator at 37°C and 5% CO2 concentration in high glucose Dulbecco’s modified Eagle medium (DMEM) with L– glutamine (Gibco) supplemented with 10% fetal bovine Calf serum and 100 U/ml penicillin-streptomycin (Himedia).
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bioRxiv - Cancer Biology 2021Quote: For this analysis 1/5 of the total EV pellet per sample was resuspended in 10 μL PBS (Gibco) and stored at 4°C for up to 7 days ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were starved for 72 hours in phenol-red free media supplemented with 5% charcoal stripped serum (CSS) (Gibco) followed by stimulation with 10nM R1881 (Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: A reverse transcription (RT) reaction was performed in 50 μL containing 5 μL of 10× SuperScript III buffer (Invitrogen), 5 μL of 50 mM MgCl2 ...
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bioRxiv - Immunology 2021Quote: ... cells were stained with 5 μM CellROX Green staining solution for 30 mins per manufacturer’s instructions (Invitrogen, cat# C10492). Six-seven different visual fields were acquired with Leica SPE confocal microscope and Mean Fluorescence Intensity (MFI ...
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bioRxiv - Cell Biology 2021Quote: ... and lifted for 3 min at 37°C using 5 mL of TrypLE Express (Invitrogen, Gibco, Cat no. #1260501). The appropriate number of cells were then spun down at 90 x g for 10 min at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... and lifted for 3 min at 37°C using 5 mL of TrypLE Express (Invitrogen, Gibco, Cat no. #1260501). The appropriate number of cells were then spun down at 90 x g for 10 min at room temperature ...
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bioRxiv - Biophysics 2021Quote: ... 5 mM DTT) and then mixed with 50 mM (final 6 mM) disuccinimidyl dibutyric urea (DSBU) (Thermo Fisher Scientific). Crosslinking reaction was incubated on ice for 2 hours and then was quenched by adding 1M (final 50 mM ...
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bioRxiv - Biophysics 2021Quote: ... passage 3-14) were cultured at 37 °C with humidified air containing 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM, Gibco) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2020Quote: Time plug-mated Pbx3−/- mice received a single intraperitoneal injection of 5-ethynyl-2’-deoxyuridine (EdU; 0,1mg/g animal; Invitrogen) at E15.5 ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were incubated for 5 min with 2 μM LysoSensor Yellow/Blue DND-160 (L7545, Invitrogen/Thermo Fisher Scientific) in extracellular Ringer’s solution at 37°C and 5% CO2 ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were incubated for 5 min with 2 μM LysoSensor Yellow/Blue DND-160 (L7545, Invitrogen/Thermo Fisher Scientific) in extracellular Ringer’s solution at 37°C and 5% CO2 ...
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bioRxiv - Cancer Biology 2020Quote: ... the slides were counterstained with DAPI for 5 min and mounted with ProLong™ Gold Antifade Mountant (Thermofisher Scientific). Slides were scanned for image acquisition using Zeiss AxioScan Z.1 and ZEN2 software.
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bioRxiv - Cancer Biology 2021Quote: ... cDNA samples were diluted 5-fold and it was performed with StepOne Plus Real Time PCR System (Applied Biosystems). The FastStart Universal SYBR Green Master Mix (Roche ...
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bioRxiv - Cancer Biology 2022Quote: ... and resuspended in flow buffer plus 5 μL of propidium iodide (PI) staining solution (10 μg mL-1, Invitrogen) per sample ...
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bioRxiv - Developmental Biology 2022Quote: ... After multiple 5-min washes in PBS and 90-min incubation with AlexaFluor-conjugated secondary Ab as above (Invitrogen) at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... Remnant villi were removed by shaking tissue pieces at 4°C in an initial 5 minute incubation PBS with 10 mM RNase-free EDTA pH 8.0 (Invitrogen). Crypts were collected and filtered through 70 µm cell strainers in subsequent fractions ...
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bioRxiv - Molecular Biology 2022Quote: ... the samples were bound to a precolumn (Acclaim C18 PepMap100, 300 μm i.d., 5 mm length; Thermo Fisher Scientific) and then eluted onto the analytical column (SilicaTip Emitter ...
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bioRxiv - Neuroscience 2022Quote: ... Cortical astrocytes were plated at a density of 2.5 × 104 to 5 × 104 cells.cm−2 in Minimum Essential Media (Gibco, MEM) supplemented with 10% Horse Serum (Gibco) ...
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bioRxiv - Cell Biology 2022Quote: ... The qPCR reactions were performed in triplicate using a QuantStudio 5 Real-time PCR System (Thermo Fisher, cat#A34322) using the following thermocycling conditions ...
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bioRxiv - Cell Biology 2022Quote: ... cells were resuspended in sorting buffer (0.2% BSA in PBS) containing 5 nM SYTOX Green Nucleic Acid Stain (catalog number S7020; ThermoFisher) to distinguish between live and dead cells ...
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bioRxiv - Biochemistry 2022Quote: ... coli cells were grown in LB media (Difco LB Broth, Miller [Luria Bertani,] Fisher Scientific, Catalog #DF0446-07-5) for reporter gene and RT-qPCR assays ...
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bioRxiv - Cell Biology 2022Quote: ... Northwestern University) cells were cultured at 37 °C with 5 % CO2 in Dulbecco’s modified eagle’s medium (DMEM, Life Technologies) containing 10 % fetal bovine serum (Seradigm ...
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bioRxiv - Cell Biology 2022Quote: ... After incubation cells were incubated for 5 min at RT with TMR-phalloidin (0.83 μg/mL) and Hoechst 33342 (1 μg/mL, Invitrogen) in PBS and washed with blocking buffer before imaging on a Zeiss Axiovert 200 equipped with an Andor Luca camera.
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bioRxiv - Biochemistry 2022Quote: ... 2 µL of 20 µM siRNA and 5 µL RNAiMAX were diluted in 400 µL OptiMEM (Thermo Fisher Scientific), mixed gently and incubated for 20 min at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... TcBDF2W92AFw (5’CGACTCCGCTGCGGTTAAAG-3’) and TcBDF2W92ARv (5’-CTTTAACCGCAGCGGAGTCG-3’).The PCR products were first cloned into the pCR2.1-TOPO vector (Invitrogen) and sequenced ...
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bioRxiv - Cancer Biology 2022Quote: ... Dispase 5 mg/ml [GIBCO], Y-27632 10.5 µM [Sigma], DNase I 10 µg/ml [Sigma], Advanced DMEM/F12 [GIBCO] ...
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HNRNPA2B1 controls an unfolded protein response-related prognostic gene signature in prostate cancerbioRxiv - Cancer Biology 2022Quote: ... Binding of SYBR green to DNA was analysed in a QuantStudio 5 Real-Time PCR system (Thermo Fisher Scientific). Reaction conditions were as follows ...
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bioRxiv - Molecular Biology 2022Quote: Cells were then counterstained with DAPI (4,6-diamidino-2-2phenylindole) in 5% BSA with 0.1% Triton X-100 and mounted with Prolong Gold Antifade mounting medium (Invitrogen). Images were then acquired with Olympus Fluoview FV1000 confocal microscope system with PLAPON 60X O SC NA 1.40 ...
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bioRxiv - Neuroscience 2022Quote: ... Each sample was concentrated over an Acclaim PepMap C18 pre-column (5µm particle size, 0.3mm ID x 5 mm length, ThermoFisher Scientific) then bound to a 50cm EasySpray C18 analytical column (2µm particle size ...
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bioRxiv - Neuroscience 2022Quote: ... Tissue sections were blocked for 30 minutes in a buffer solution containing 5% normal donkey serum (Thermo Fisher Scientific) and 0.5% Triton X-100 in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... washed 3 times with PBS 5 min each and mounted on glass slides using Prolong Diamond antifade reagent (Invitrogen). We prepared antibodies in PBS containing 3% BSA ...
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bioRxiv - Systems Biology 2022Quote: ... peptides were eluted from the filters after digestion using ABC and 5% acetonitrile (ACN) (Thermo Fisher, Cat. No. 10489553) / 0.1% formic acid (FA ...
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bioRxiv - Immunology 2022Quote: ... IgG was eluted from the resin in 5 × 2 mL fractions using IgG elution buffer (Pierce ThermoFisher, catalog #21004). Fractions were buffer exchanged into PBS ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated with primary antibody O/N at 4°C (5% FBS, 0.5% Tx, 0.2% gelatine in PBS; GFP (1:500, ThermoFisher Scientific). Slices were then repeatedly washed with PBS-0.5% Tx ...
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bioRxiv - Microbiology 2022Quote: ... The cell suspension was then inoculated on LBN agar plates containing 5 % (v/v) defibrinated sheep blood (ThermoFisher Scientific) for haemolysin assay and 1 % (w/v ...
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bioRxiv - Genomics 2022Quote: RNA from 5 frozen islet donor preps acquired via the IIDP was isolated using TRIzol (15596018, Thermo Fisher Scientific). All RNA integrity numbers (RIN) ...
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bioRxiv - Cell Biology 2022Quote: Isolated colonic crypts or organoids were loaded at RT with Fura-2/AM (5 μm, 2h; Thermo Fisher Scientific) in HBS ...
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bioRxiv - Microbiology 2022Quote: ... DNA was digested from 5-10µg of total RNA per sample using the TURBO DNA-free kit (ThermoFisher Scientific) and purified using RNA Clean and ConcentratorTM-5 kit ...
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bioRxiv - Immunology 2022Quote: ... permeabilized with 0.1% triton in PBS for 10 min and blocked with 5% normal goat serum (ThermoFisher Scientific 31873) in PBS for 45 min prior to staining with primary antibodies (5 hrs RT or 21 hrs at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... HCT-8 cells were cultured in 25 cm flasks with 5 ml of complete media (RPMI-1640 media [Gibco/Thermo Fisher Scientific ...
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bioRxiv - Genetics 2022Quote: ... LCLs were maintained at 37°C in a 5% CO2 atmosphere and were grown in RPMI 1640 media (Invitrogen) supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Physiology 2022Quote: Amplicons for dsRNAs were designed using the SnapDragon dsRNA design tool (https://www.flyrnai.org/snapdragon) and in vitro transcribed (IVT) using MEGAscript® T7 Transcription Kit (Cat# AMB1334-5, ThermoFisher). IVT reactions were carried out as per the protocol provided by the DRSC (available at ...
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bioRxiv - Cell Biology 2022Quote: ... originally obtained from the ATCC) and were cultured at 37 °C and 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM, Gibco) supplemented with 10 % fetal calf serum (FCS ...
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bioRxiv - Cell Biology 2022Quote: ... originally obtained from the ATCC) and were cultured at 37 °C and 5% CO2 in McCoy’s 5A medium (Cat# 16600082, Gibco) supplemented with 10 % fetal calf serum (FCS ...
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bioRxiv - Developmental Biology 2022Quote: ... was used to detect amplification of the target sequences on a QuantStudio 5 Real-Time PCR system (Applied Biosystems). Data are represented by boxplots as above ...
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bioRxiv - Cell Biology 2022Quote: ... non-IR and IR animals were systemically injected with 0.9% saline containing 0.25 mg per 25 g of body weight of 5-ethynyl-2’-deoxyuridine (EdU, Thermofisher Scientific) followed by a 2 hour or 7 days chase before sacrifice ...