Labshake search
Citations for Thermo Fisher :
8701 - 8750 of 10000+ citations for 5 NITROFURFURYL ALCOHOL since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
Bidirectional neuronal migration coordinates retinal morphogenesis by preventing spatial competitionbioRxiv - Developmental Biology 2021Quote: ... iPSCs and developing organoids were cultured at 37°C and 5% CO2 in a humidified incubator (Thermo Fisher Scientific).
-
Reconstitution of prospermatogonial specification in vitro from human induced pluripotent stem cellsbioRxiv - Developmental Biology 2020Quote: ... Tissues were centrifuged at 200 g for 5 min and washed with Hank’s Balanced Salt Solution (Thermo Fisher Scientific). Tissues were then digested with 0.25% trypsin/EDTA and DNase I for 8 min at 37 °C ...
-
bioRxiv - Cancer Biology 2021Quote: ... Precipitated RNAs were quantified using Taqman miRNA assays with custom primers for detection of 5’-tRFCys (Thermo Fisher Scientific).
-
bioRxiv - Cancer Biology 2021Quote: ... LDHC expression was quantified by specific 5′FAM-3′MGB Taqman gene expression primer/probe sets (Hs00255650_m1, Applied Biosystems). MAP1B expression was quantified using primers for SYBR-based qPCR (F ...
-
bioRxiv - Cancer Biology 2020Quote: ... samples were blocked in PBS +5% BSA +0.3% Triton X-100 in PBS and stained using an AlexaFluor488-labeled ZO-1 (#339188, Invitrogen) or ROR2 (#FAB20641G ...
-
bioRxiv - Cell Biology 2019Quote: ... (1.5 μl microsomes in 48.5 μl PBS) in the absence or presence of 0.1% Triton X-100 (Thermo Fisher). The solution was added into a CELLSTAR™ 96 Well Polystyrene Flat Bottom Cell Culture Microplate (Greiner Bio-One ...
-
bioRxiv - Cell Biology 2020Quote: ... Cells were pelleted at 300 x g for 5 minutes and resuspended in freezing medium (fetal bovine serum (Gibco), 10% v/v DMSO) ...
-
bioRxiv - Cell Biology 2020Quote: ... Melanoma cell line 501mel was grown in 5% CO2 at 37°C in RPMI w/o HEPES (Gibco, Invitrogen) supplemented with 10% FCS and Gentamycin ...
-
bioRxiv - Cell Biology 2020Quote: ... All cells were maintained in 1:1 Ham’s F12 Dulbecco’s modified Eagle’s medium (FDV) containing 5% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 5ml/500ml 100X penicillin-streptomycin (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2021Quote: ... the pDyn3 plasmid containing the human dynein genes or the pFastBac plasmid containing full-length Lis1 was transformed into DH10EmBacY chemically competent cells with heat shock at 42°C for 15 seconds followed by incubation at 37°C for 5 hours in S.O.C media (Thermofisher scientific). The cells were then plated on LB-agar plates containing kanamycin (50 μg/ml) ...
-
bioRxiv - Genetics 2021Quote: ... in PBS for 1 minute (min), centrifuged (600 g, 5 min) and then incubated in Colagenase (0.5 mg/ml) (Gibco) in RPMI medium (Lonza ...
-
bioRxiv - Genetics 2020Quote: ... qPCR were carried out on the generated cDNA (diluted at 1/5) using PowerUp SYBR master mix (Thermofisher Scientific) in a final volume of 10 μl and 40 cycles of amplification on QuantStudio3 Real-Time PCR System (ThermoFisher) ...
-
bioRxiv - Genomics 2021Quote: HEK-293 cells (ATCC CRL-1573) were grown at 37°C in 5% CO2 in Dulbecco’s Modified Eagle Medium (Gibco) supplemented with 10% FBS (Omega Scientific ...
-
bioRxiv - Genomics 2020Quote: The eluted cDNA:mRNA hybrids (15 μl) were combined with second strand mix [2 μl 5× First Strand Buffer (Invitrogen), 2.31 μl Second Strand Buffer (Invitrogen) ...
-
bioRxiv - Microbiology 2021Quote: ... except that BME vitamin solution 100x (Bioconcept 5-20K00-H) was used instead of vitamin mix 100x (Invitrogen 010144) and 1% Pen/Strep stock solution (Bioconcept 4-01F00-H ...
-
bioRxiv - Microbiology 2021Quote: Green monkey kidney fibroblast-like Cos7 cells were cultured at 37 °C with 5% atmospheric CO2 in Dulbecco’s Modified Eagle Medium (DMEM; Gibco, Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2020Quote: ... CRL-1586) were maintained at 37°C and 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM, Hyclone or GIBCO) supplemented with 10% Fetal Bovine Serum (FBS ...
-
bioRxiv - Microbiology 2021Quote: ... Cells were then permeabilized using PBS with 0.2% Triton X-100 for 15 min and blocked with 5% FBS (Gibco), followed by treatment with the primary anti-JEV prM antibody (4 ...
-
bioRxiv - Immunology 2020Quote: Spleens were removed from each mouse and placed in 5 mL of sterile medium of RPMI (Gibco Cat # 22400105), HEPES (Hyclone Cat# SH30237.01) ...
-
bioRxiv - Microbiology 2021Quote: ... approximately 500 10-days old flies were squashed for 5 minutes in 10ml Schneider’s Insect Medium (Thermo Fisher Scientific) using glass beads ...
-
bioRxiv - Immunology 2020Quote: ... Pen/Strep and 5% IgG-depleted FBS (CCU, The Pirbright Institute or GIBCO, ultra-low IgG, ThermoFisher Scientific, UK).
-
bioRxiv - Immunology 2020Quote: ... live CD3+CD8+) were sorted from a C57BL/6Thy1.1 mouse as responder cells and labeled with 5 μM CellTrace Violet (Invitrogen). Whole splenocytes from 6-8 week-old ...
-
bioRxiv - Immunology 2020Quote: ... All cell lines were cultured in a humidified 37°C incubator (5% CO2) in Dulbecco’s Modified Eagle Medium (Gibco) supplemented with 10% Fetal Bovine Serum and 1% Penicillin/Streptomycin ...
-
bioRxiv - Molecular Biology 2021Quote: ... then co-transfected with 0.25 µg of pcDNA™5/FRT/TO and 2.25 µg of pOG44 Flp recombinase expression plasmid (Invitrogen) using Lipofectamine LTX with Plus Reagent (Invitrogen) ...
-
bioRxiv - Molecular Biology 2021Quote: ... and Il36A_rev (5′-CAGTTCTTGGGTCAGAATGAGTG-3′) and subsequent cloning into pJET1.2/blunt vector as described by the manufacturer (ThermoFisher Scientific). The DNA fragment encoding mouse C/EBPβ residues 221–296 (bZIP domain ...
-
bioRxiv - Microbiology 2020Quote: Samples consisting of either solubilized membranes or purified membrane complexes were mixed with 5% G-250 sample additive (Invitrogen), to a final concentration of ~0.2% ...
-
bioRxiv - Microbiology 2020Quote: ... 2002) were maintained at 37 °C and 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM, Fisher Scientific, cat. #11995065) supplemented with 0.1 mM nonessential amino acids (NEAA ...
-
bioRxiv - Microbiology 2020Quote: ... African green monkey kidney epithelial Vero-E6 cells (ATCC, CRL-1586) were maintained at 37°C and 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM, Gibco) supplemented with 10% Fetal Bovine Serum (FBS ...
-
bioRxiv - Microbiology 2020Quote: Spinner-adapted L cells were grown in suspension culture in Joklik’s minimum essential medium (US Biological) supplemented to contain 5% fetal bovine serum (FBS) (Gibco), 2 mM L-glutamine (Corning) ...
-
bioRxiv - Microbiology 2020Quote: ... Huh-7 .5 and BHK-21 cells were cultured in Dulbecco’s minimal essential medium (DMEM) (Gibco /Thermo Fisher Scientific) supplemented with 2mM L-glutamine (Gibco /Thermo Fisher Scientific) ...
-
bioRxiv - Microbiology 2020Quote: ... Huh-7 .5 and BHK-21 cells were cultured in Dulbecco’s minimal essential medium (DMEM) (Gibco /Thermo Fisher Scientific) supplemented with 2mM L-glutamine (Gibco /Thermo Fisher Scientific) ...
-
bioRxiv - Microbiology 2020Quote: Human cervical epithelial adenocarcinoma HeLa cells were cultured at 37° C with 5% atmospheric CO2 in Dulbecco’s Modified Eagle Medium (DMEM; Gibco, Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2020Quote: ... Blocks were sectioned to 5 μm thickness using a Leica microtome (RM2145) and applied to Ultrastick slides (Thermo Scientific). Sections were stored at room temperature until CLASI-FISH was performed.
-
bioRxiv - Immunology 2021Quote: B16-F10 cells (ATCC, CRL-6475) were purchased and cultured at 37°C in 5% CO2 in DMEM (Invitrogen), 10% FCS (Benchmark) ...
-
bioRxiv - Immunology 2020Quote: mRNA from 2-5 x 104 sorted cells was captured with 12 μl of Dynabeads oligo(dT) (Life Technologies), washed ...
-
bioRxiv - Immunology 2021Quote: ... 100mL cultures of Expi293F cells at a density of 2.5×106 cells/mL were transiently transfected with 50µg each heavy and light chain encoding plasmids and Expifectamine (Invitrogen) per manufacturer’s protocol ...
-
bioRxiv - Immunology 2021Quote: HeLa and MelJuSo cell lines were maintained at 37°C/5% CO2 in Gibco Iscove’s Modified Dulbecco’s Medium (IMDM, Life Technologies-Invitrogen) supplemented with 10% fetal bovine serum (FBS ...
-
bioRxiv - Immunology 2021Quote: ... RNA samples were first incubated at 65°C for 5 min in the presence of 0.5 mM dNTPs and 2.5 µM oligo(dT)20 (Life Technologies-Invitrogen). Subsequently ...
-
bioRxiv - Immunology 2021Quote: ... which were then transfected into Expi293F cells growing at 37 in 5% CO2 atmosphere using ExpiFectamine reagent (Thermofisher Scientific). Proteins were purified by strep-tag affinity and subsequently size exclusion chromatography.
-
bioRxiv - Microbiology 2021Quote: ... Exponential phase starter cultures (OD600∼ 0.5 - 1.5) were diluted to OD600 = 0.01 and grown in 96-well Nunclon Delta surface clear plates (Thermo Scientific) with 150 μL per well ...
-
bioRxiv - Immunology 2021Quote: ... replicate-wells of human IgG (40, 20, 10, 5, and 2.5 ng/µl in PBS with 10% glycerol) and AlexaFlour-555-Hydraside (Invitrogen, at 1 ng/µl in 178 mM phosphate buffer ...
-
bioRxiv - Immunology 2021Quote: ... Slices were maintained in a humidified incubator with 5% CO2 at 37°C with 1 ml MEM (Gibco, 31095209) containing 20% (v/v ...
-
bioRxiv - Microbiology 2021Quote: ... Equal concentrations of lysate (5 µg) were analyzed by reducing SDS-PAGE and transferred to a nitrocellulose membrane (Invitrogen). Membranes were washed with tris buffered saline (pH 7.6 ...
-
bioRxiv - Molecular Biology 2021Quote: ... samples were incubated with secondary antibodies conjugated to HRP at 1:20,000 in 5% milk/1x TBST for 1 hour (goat anti-mouse-HRP, 32430; ThermoFisher Scientific and goat anti-rabbit-HRP ...
-
bioRxiv - Molecular Biology 2021Quote: ... A total of 5 μg protein was run on a NuPage™ 4-12% Bis-Tris Protein Gel (Invitrogen) using MOPS buffer according to manufacturer instructions and subsequently transferred to a nitrocellulose membrane according to the NuPage™ manufacturer's protocol (Invitrogen) ...
-
bioRxiv - Immunology 2021Quote: ... Then, 5 x106 cells were washed in PBS (HyClone Laboratories, Logan, UT, USA) containing 1 % BSA (Thermo Fisher Scientific) and stained in 100 μL PBS and BSA 1 %-containing Antibody cocktail ...
-
bioRxiv - Immunology 2020Quote: Chloroquine diphosphate salt (CQ) (98%) and adenosine 5’-monophosphate sodium salt hydrate (AMP) (99%) were purchased from ACROS Organics™ ...
-
bioRxiv - Immunology 2020Quote: ... Plates were blocked for 1 hr at room temperature with 200 μl 5% Blocker™ BLOTTO (Thermo Scientific, #37530) and washed three times with 200 μl PBS-T ...
-
bioRxiv - Immunology 2021Quote: ... were diluted in two-fold dilution series from 1:5 to 1:5120 in Eagle’s Minimum Essential Medium (Gibco) with 5% FCS (Thermo Fisher Scientific) ...
-
bioRxiv - Immunology 2021Quote: ... lysed in 1X Novex buffer and 5-7.5×105 cells equivalents were resolved on 4-12% NuPAGE gel (Invitrogen) in MOPS buffer ...