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Citations for Thermo Fisher :
8401 - 8450 of 10000+ citations for 5 NITROFURFURYL ALCOHOL since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... permeabilised with Triton 0.1% X-100 for 5 minutes at room temperature and stained with Alex Fluor 546 Phalloidin (Invitrogen) 1:2000 for one hour and DAPI (Invitrogen ...
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bioRxiv - Biophysics 2020Quote: ... and MA104 (ATCC CRL-2378.1) cells were maintained at 37°C in a fully humidified atmosphere with 5% CO2 in DMEM (Invitrogen) and M199 (Gibco ...
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bioRxiv - Cell Biology 2021Quote: ... Isolated EVs in PBS (5 - 10 μg protein) were acidified with trichloroacetic acid (TCA; Fisher Scientific, Cat #A322-100) (10% vol:vol final ...
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bioRxiv - Cancer Biology 2020Quote: All DLBCL cell lines were cultured at 37°C in 5% CO2-containing humidified incubators using Advanced RPMI (Gibco) containing 5% fetal bovine serum (Hyclone or Seradigm ...
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bioRxiv - Cell Biology 2021Quote: ... the stromal layer was washed with PBS and was subject to mild trypsinisation with 1:5 dilution of 0.25% Trypsin 1 mM EDTA (Thermofisher) to remove attached residual leukaemic cells before completely detaching stromal cells with 0.25% Trypsin-EDTA.
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bioRxiv - Cell Biology 2021Quote: ... amplification of the 1.4 kb of the 5’ UTR region was carried out using Phusion High Fidelity polymerase (ThermoFisher) with the primers shown in Supp ...
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bioRxiv - Cell Biology 2021Quote: ... cells and cervical adenocarcinoma epithelial (HeLa) cells were cultured at 37°C with 5% atmospheric CO2 in Dulbecco’s Modified Eagle Medium (DMEM; Gibco, Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: HEK 293T cells were cultured at 37°C in 5% CO2 in DMEM+ GlutaMax (Thermo Fisher, cat. no. 10566016) with 10% fetal bovine serum (Sigma ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Three tadpoles per stage (1, 2, 5, 7, and 8 weeks after hatching) were fixed in an RNAlater (Ambion) solution ...
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bioRxiv - Neuroscience 2020Quote: ... Cell cultures were maintained in an incubator with 5% CO2 (Forma Series II Water Jacket CO2 Incubator, Thermo Scientific) at 37°C ...
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bioRxiv - Bioengineering 2020Quote: ... The marrow was flushed out of the bones via 2 - 5 mL 4 °C DPBS (Dulbecco’s Phosphate-Buffered Saline; Cat. No. 21-031-CV; Gibco) injection ...
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bioRxiv - Biochemistry 2021Quote: ... After loading onto a trapping microcolumn Acclaim PepMap100 C18 (20 mm x 100 μm ID, 5 μm, Thermo Scientific), peptides were separated on a reversed-phase Easy Spray C18 column (50 cm x 75 μm ID ...
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bioRxiv - Biochemistry 2021Quote: ... were cultured at 37°C and 5% CO2 in Dulbecco’s modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin/streptomycin (Gibco) ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was diluted to 50 µM and labelled with a 5:1 molar excess of fluorophore:protein with either AlexaFluor 488 C5- Maleimide (Invitrogen) (AF488) ...
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bioRxiv - Biochemistry 2020Quote: StNFeoB proteins (2-5 µM each) were incubated with a range of substrate (GTP for molecular biology, Thermo Scientific) concentrations (25 µM to 5000 µM) ...
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bioRxiv - Cell Biology 2021Quote: ... ALI-differentiation medium was removed and refreshed with ALI-differentiation medium supplemented with DAPT (5 mM, Fisher Scientific #15467109) for 14-18 days ...
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bioRxiv - Cell Biology 2021Quote: ... 5% of total RNA was DNase-treated in 50 µl reactions with TURBO DNase (Thermo Fisher Scientific, Cat# AM1907) for 30 min at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... MSn fragmentation data of 5 was acquired using an ESI-LTQ Orbitrap XL (Thermo Fisher Scientific GmbH, Bremen, Germany) MS spectrometer.
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bioRxiv - Systems Biology 2021Quote: ... A 1:5 dilution of this protein solution was quantified using a Pierce BCA protein assay kit (Thermo scientific). For analysis of the relative composition of amino acids ...
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bioRxiv - Plant Biology 2020Quote: ... 5 μg of total RNA was treated with TURBO DNA-free™Kit (Invitrogen, Thermo Fisher Scientific, MA, USA) to remove any potential genomic DNA contaminants ...
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bioRxiv - Plant Biology 2020Quote: ... 5 μg of total RNA was treated with TURBO DNA-free™Kit (Invitrogen, Thermo Fisher Scientific, MA, USA) to remove any potential genomic DNA contaminants ...
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bioRxiv - Microbiology 2020Quote: ... African green monkey kidney epithelial Vero E6 cells were grown in Dulbecco’s modified Eagle’s medium (DMEM) with 5% fetal bovine serum (FBS; HyClone Laboratories, South Logan, UT) and 1% antibiotic/ streptomycin (Gibco). Human lung adenocarcinoma epithelial Calu-3 2B4 cells were maintained in a high-glucose DMEM supplemented with 10% FBS and 1% penicillin/streptomycin at 37°C with 5% CO2 ...
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bioRxiv - Microbiology 2020Quote: ... The membranes were blocked with 5% (w/v) non-fat milk in TBS- Tween-20 (TBST) buffer (Thermo Scientific) for 1 hour at room temperature ...
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bioRxiv - Systems Biology 2021Quote: ... Dried lipid fractions (~5 mg) were dissolved (isopropanol/dichloromethane 1:1, 300 μL) for accurate mass LC-MS (ThermoFisher Q exactive with Dionex Ultimate 3000 sample handler and Waters acquity UPLC BEH C18 with 1·7 μm particle size LC column ...
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bioRxiv - Microbiology 2020Quote: ... ATCC) monolayers in Dulbecco modified Eagle medium (DMEM) supplemented with 5% heat-inactivated fetal bovine serum (FBS) (Gibco/Invitrogen), 100 U/ml penicillin ...
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bioRxiv - Microbiology 2020Quote: ... a DNA fragment comprising the entire coding region of kpfR plus approximately 550 pb of 3’ and 5’ flanking regions (Table S4 in the supplemental material) was inserted on pCR2.1-TOPO vector (Invitrogen) previously cloned with erythromycin-resistance gene ...
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bioRxiv - Plant Biology 2020Quote: ... two-day-old etiolated seedlings were incubated in half strength OS medium containing 5 µM FM4-64 (Thermo Fisher) for 1 h with blue light treatment ...
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bioRxiv - Neuroscience 2020Quote: Neuro2a and HEK293T cells were obtained from ATCC and maintained in Dulbecco’s modified Eagles medium supplemented with 5% fetal bovine serum and Penicillin/Streptomycin (Gibco). 3 x 105 cells were seeded in 6-well plate and maintained by indicated times in DMEM cell culture media supplemented with 5% bovine fetal serum and non-essential amino acids ...
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bioRxiv - Microbiology 2020Quote: ... Cell envelopes were stained by adding 5 µL of 1 mg/mL FM™ 4-64FX (Thermo Fisher Scientific) and non-viable cells stained with 1 µL of the LIVE/DEAD™ cell dye (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2020Quote: Mouse midbrains (n = 5-6 per group per age) were collected and RNA was isolated using TRIzol Reagent (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: OMVs were boiled at 95°C for 5 minutes with lithium dodecyl sulfate (LDS) sample buffer (4X Bolt; Invitrogen) and run on a NuPAGE 12% Bis-Tris gel (Invitrogen ...
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bioRxiv - Systems Biology 2021Quote: ... The samples were then further fixed with 1.5 mM BS(PEG)5 (PEGylated bis(sulfosuccinimidyl)suberate) (Thermo Scientific A35396) in 1× PBS at room temperature for 30 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... Ganglia were dissociated by trituration using a fire polished Pasteur pipette, followed by centrifugation (5 minutes, 160 x g) and trituration in wash buffer (DMEM Ham’s F-12, Gibco, 10565-018 ...
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bioRxiv - Genetics 2021Quote: ... PCR reactions contained 1 μl of 1:10 diluted lysate and 5 μl premixed AmpliTaq-Gold (0.3U, Life Technologies) with 0.26 μM of target-specific- and an additional fluorescent forward primer ...
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bioRxiv - Genomics 2021Quote: ... Samples were transferred to 1.5 mL safe-lock tubes containing one scoop of acid-washed glass beads (<106μM) and 1 mL TRIzol (Invitrogen) containing 20μg/mL GenElute-LPA (Sigma-Aldricht ...
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bioRxiv - Genetics 2021Quote: ... urartu was labelled by nick translation with ChromaTide™ Alexa Fluor™ 488-5-dUTP (Invitrogen; C11397; coloured green), (2 ...
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bioRxiv - Neuroscience 2020Quote: ... All neurons were maintained at 37°C and 5 % CO2 in glia- and cortex-conditioned Neurobasal-A (Life Technologies) supplemented with B27 and GlutaMax ...
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bioRxiv - Neuroscience 2020Quote: ... Peptides were washed and preconcentrated on PepMap™ C18 precolumns (5 mm x 300 μm inner diameter; Thermo Scientific) and separated using AcclaimPepMap™ RSLC columns (50 cm x 75 μm inner diameter ...
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bioRxiv - Microbiology 2020Quote: The Vero cells were maintained at 37℃ under 5% CO2 in Dulbecco’s modified Eagle essential medium (DMEM) supplemented with 10% heat-inactivated fetal bovine serum (FBS, Gibco), 10 mM HEPES and 1% penicillin/streptomycin ...
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bioRxiv - Microbiology 2020Quote: ... Approximately 5 mg of cecum tissue was removed and placed in RNAlater and stored at −80°C (Thermo Fisher). The remaining cecum (approximately two thirds ...
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bioRxiv - Neuroscience 2020Quote: ... Cell-attached recordings were performed with aCSF + 5 μM Alexa Fluor® 488 nm (Molecular Probes Cat# A-10440) or 594 nm (Molecular Probes Cat# A-10442 ...
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bioRxiv - Microbiology 2021Quote: ... A trap column (5 μm, 100 Å, 100 μm × 2 cm, Acclaim PepMap 100 nanoViper C18; Thermo Fisher Scientific) and an analytical column (2 μm ...
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bioRxiv - Neuroscience 2020Quote: ... Eyes were removed quickly using Dumont’s forceps #5 and put in ice-cold Hibernate A medium without calcium and magnesium (Invitrogen). Under a dissection scope ...
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bioRxiv - Pathology 2021Quote: ... were grown at 37°C in a humidified 95% air/5% CO2 incubator in Dulbecco’s Modified Eagle Medium (DMEM; Gibco) supplemented with 10% v/v heat-inactivated fetal bovine serum ...
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bioRxiv - Microbiology 2021Quote: ... Cells were permeabilized using PBS with 0.2% Triton X-100 for 15 min and blocked with 5% FBS (Gibco), followed by treatment with the primary antibody anti-SARS-CoV-2 NP (GeneTex GTX635678 ...
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bioRxiv - Systems Biology 2020Quote: ... Primary fibroblasts were expanded and cultured at 37°C in 5% CO2 in fibroblast medium (Dulbecco’s Modified Eagle Medium (DMEM) + Glutamax (Gibco) with 100 mg/mL penicillin/streptomycin (Sigma) ...
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bioRxiv - Microbiology 2020Quote: ... and African green monkey kidney epithelical BS-C-1 cells (ATCC, CCL-26) were maintained at 37°C and 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM, Gibco) supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Microbiology 2020Quote: ... for 1hour and labelled with 1μM CFSE (CFSElow) or 5 μM CFSE(CFSEhigh) respectively following manufacturer’s instructions (Life Technologies). A mixture of 2×106 CFSElow and CFSEhigh splenocytes were intravenously injected to gender matched naïve mice or the mice which had been intranasally infected 8 days ago with H1N1 or H5N1(2:6 ...
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bioRxiv - Microbiology 2020Quote: ... The 10 μl reactions contained 5 μl of SYBR Green ROX qPCR (Thermo Fisher Scientific Inc., Wilmington, DE, USA), 0.2 μl of bovine serum albumin (Thermo Fisher Scientific Inc. ...
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bioRxiv - Cancer Biology 2020Quote: ... Freshly dissected tissues were incubated for 5 min in 50 nM LysoTracker Red DND-99 (Life Technologies, Cat# L7528) in PBS ...