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Citations for Thermo Fisher :
8201 - 8250 of 10000+ citations for 5 NITROFURFURYL ALCOHOL since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... Immunoblots were incubated for 5 minutes at room temperature with Supersignal West Femto maximum sensitivity substrates (Thermo Scientific) and developed using an ImageQuant 500 CCD imaging system (Cytiva).
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bioRxiv - Microbiology 2023Quote: ... The reactions were then run on a QuantStudio 5 Real-Time PCR Instrument (A28133, Applied Biosystems, Waltham, MA).
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bioRxiv - Molecular Biology 2023Quote: ... All cells were grown at 37°C with 5% CO2 and in Dulbecco’s modified Eagle’s medium (Gibco, 11995065) supplemented with 10% fetal bovine serum and penicillin-streptomycin-glutamine (Gibco ...
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bioRxiv - Neuroscience 2023Quote: ... cells were incubated with the same medium with the addition of 5 μg mL−1 zeocin (ThermoFisher Scientific). For neuronal maturation ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were washed 3 x 5 min with TBST and developed using chemiluminescent substrate (Thermo Scientific SuperSignal West) and imaged using a BioRad Chemidoc.
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bioRxiv - Microbiology 2023Quote: ... and HEK293T cells were grown at 37°C with 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco) supplemented with 10% fetal bovine serum (Atlanta Biologicals ...
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bioRxiv - Cell Biology 2023Quote: ... Readout probes tagged with Alexa 488 and Alexa 647 at their 5′ ends were synthesized by Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... rtf1-e3 gRNA (target sequence: 5’-GGAAGAAGGGGAAACCGAGCAA-3’) was synthesized using the MEGAshortscript T7 Transcription Kit (ThermoFisher Scientific) and purified using the RNeasy Mini Kit (Qiagen) ...
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bioRxiv - Immunology 2023Quote: Freshly isolated neutrophils were stained with CellTracker™ Green 5-chloromethylfluorescein diacetate (CMFDA) dye (Thermo Fisher scientific, USA) at 5µM in serum free RPMI-1460 (SFM ...
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bioRxiv - Genomics 2023Quote: ... Moran) were cultured at 37°C and 5% CO2 in HeLa complete medium (DMEM, Life Technologies, Cat# 11960044) supplemented with 10% FBS (Life Technologies ...
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bioRxiv - Microbiology 2023Quote: ... cells were cultured at 37 °C and 5% CO2 in Dulbecco’s modified Eagle’s medium with glutamine (DMEM) (Gibco) supplemented with 10% (v/v ...
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bioRxiv - Neuroscience 2023Quote: ... or for 30 min with 5 µM CellROX™ Green Flow Cytometry Assay Solution (Thermo Fisher Scientific, C10492) in fresh DMEM/F12+Glutamax w/o FBS and P/S ...
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bioRxiv - Neuroscience 2023Quote: ... For IHC we permeabilized and blocked sections with blocking solution (PBS containing 5% normal goat serum [Thermo Fisher Scientific # 10000C] and 0.1% Triton X-100 [Millipore-Sigma # X100-100ML] ...
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bioRxiv - Neuroscience 2023Quote: HEK293T cells (ATCC) were grown in a 37°C and 5% CO2 atmosphere in DMEM (Gibco Cat# 11995065) + 10% FBS ...
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bioRxiv - Genetics 2023Quote: ... Cells were then blocked with 2 mL blocking solution made of 5% Normal Goat Serum (cat#PCN5000 Thermofisher) and 0.05% Triton X-100 in PBS for 2 hours at RT or 4°C on a shaker.
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bioRxiv - Genomics 2023Quote: ... Real-time PCR was performed using a QuantStudio 5 Real-Time PCR System (Applied Biosystems, Foster City, CA). The PCR cycling conditions were as follows ...
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bioRxiv - Microbiology 2023Quote: ... and pYK-JRCSF HIV-1 expression plasmid in a 5:1 mass ratio with Lipofectamine 2000 (Life Technologies) according to the manufacturer’s manual.
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bioRxiv - Molecular Biology 2023Quote: ... Each ATO pellet was resuspended in 5 µL of RB-27 medium comprising of RPMI 1640 (Thermofisher Scientific), 4% B-27 supplement (Thermofisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... The cell supernatants were discarded at 5 h after seeding and replaced with Neurobasal plus Medium (Gibco, A3582901) containing 2% B-27 Plus (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... authenticated using STR profiling) were cultivated in DMEM supplemented with 5 % horse serum (Thermo Fisher Scientific, cat.no. 16050122), 1% penicillin-streptomycin ...
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bioRxiv - Cell Biology 2023Quote: ... Sections were thawed at room temperature and washed three times for 5 minutes in DPBS (Gibco, 14190–094). Sections were then counterstained for 5 minutes with Hoechst 33342 (1μg/mL ...
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bioRxiv - Cell Biology 2023Quote: ... Peritoneal Lavage cells were centrifuged at 1200 rpm for 5 min and resuspended in alpha MEM (Gibco; 12571063) media supplemented with 10% fetal bovine serum (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... A final wash in PBS for 5 mins was carried out before mounting in ProLong Gold (Invitrogen, P36930). Slides were stored at −20°C until imaged.
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Self-organised pattern formation in the developing neural tube by a temporal relay of BMP signallingbioRxiv - Developmental Biology 2023Quote: ... then washed 3x for 5-10min with PBST and mounted in ProLong Antifade Mounting medium (Thermo Fisher Scientific).
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bioRxiv - Biochemistry 2023Quote: ... Off-line fractionated samples (5 μl aliquot of 15 μl sample) were injected via an autosampler (Thermo Scientific) onto a 5 μl sample loop which was subsequently eluted onto an Acclaim PepMap 100 C18 HPLC column (75 μm x 50 cm ...
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bioRxiv - Biochemistry 2023Quote: ... were cultured at 37 °C under a 5% CO2 humidified atmosphere in Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco), supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2023Quote: ... for 5 min followed by destaining with Tris-buffered saline-0.05% Tween 20 (TBST) (Thermo Scientific, J77500.K2). The membranes were blocked for 1 h at RT with 5% bovine serum albumin (BSA ...
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bioRxiv - Bioengineering 2023Quote: Protein samples (5 µg protein) were mixed with either 4 x LDS sample buffer (Thermo-Fisher Life Technologies) or 4x LDS sample buffer + 50 mM dithiothreitol for SDS-PAGE analysis under non-reducing or reducing conditions ...
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bioRxiv - Bioengineering 2023Quote: ... we tail vein injected a 50 µL volume of bovine FITC-albumin (5 mg/ml, ThermoFisher Scientific, A23015) approximately 10 minutes before intracardiac injection of single cells to label the blood vessel lumens.
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bioRxiv - Biophysics 2023Quote: ... supplemented with 5% (v/v) heat-inactivated fetal bovine serum (FBS) and 1% (v/v) antibiotic-antimycotic (Gibco). HEK293S GnTI- suspension cells (ATCC CRL-3022 ...
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bioRxiv - Molecular Biology 2023Quote: ... Readout probes tagged with Alexa 488 and Alexa 647 at their 5’ ends were synthesized by Thermo Scientific, while those labeled with ATTO565 at the 5’ end was synthesized by Sigma Aldrich ...
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bioRxiv - Genomics 2023Quote: ... then heated at 100°C for 5 minutes and loaded onto a 4-12% polyacrylamide gel (ThermoFisher NP0322). Transfer onto a PVDF membrane was performed in 1X NuPage Transfer Buffer (ThermoFisher NP00061 ...
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bioRxiv - Cancer Biology 2023Quote: ... 1% penicillin/streptomycin and 10 mM HEPES (pH 7.4)) with 5 µm CellROX Green (Thermo Fisher Scientific, C10444). Cells were washed with staining medium and re-suspended in 4’,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Cell Biology 2024Quote: ... with added 5 mL (1% v/v) P/S (Catalog: 15140-122, Thermo Fisher Scientific, Waltham, MA, USA), 60% Knock-out Serum (Catalog ...
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bioRxiv - Cell Biology 2024Quote: ... Slides were washed 3 × 5 min in PBS and embedded into ProLong Diamond Antifade Mountant (Thermo Fisher Scientific). For control experiments ...
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bioRxiv - Microbiology 2024Quote: Human 293F cells were maintained at 37°C with 5-8% CO2 in FreeStyle 293 Expression Medium (ThermoFisher) supplemented with penicillin and streptomycin ...
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bioRxiv - Microbiology 2024Quote: ... Liquid samples (5 μL) were injected on a Thermo Scientific Easy nLC 1000 (Thermo Fisher Scientific, Waltham, MA). MS detection was conducted using a Q-Exactive Orbitrap mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: HEK293T cells with grown overnight in Opti-MEM Reduced Serum media with 5% FBS (51985-034, Thermo Fisher) to 90% confluence ...
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bioRxiv - Neuroscience 2024Quote: A 5-ethynyl-uridine (EdU)-labeling experiment was conducted using the Click-iT EdU Imaging Kits by Invitrogen, adhering to the provided manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2024Quote: ... Excess mantGDP and MgCl2 was removed by buffer exchange with a 5 mL Zeba spin desalting column (ThermoFisher) following standard procedures ...
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bioRxiv - Cancer Biology 2024Quote: ... We then incubated membranes with secondary antibody (in 5% Blocking Buffer) for 1 hour at room temperature (Invitrogen cat ...
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bioRxiv - Neuroscience 2024Quote: ... These reactions were run in a ThermoFisher QuantStudio 5 Real-Time PCR system (Applied Biosystems, Foster City, California). All data were analyzed using the comparative Ct method (2-ΔΔCt ...
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bioRxiv - Bioengineering 2024Quote: ... These cell were grown at 37 °C and 5% CO2 and routinely passaged using TrypLE™ Express (Gibco).
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bioRxiv - Bioengineering 2024Quote: ... 5 µL of sample were mixed with 2 µL of 1 U/µL DNase I (ThermoFisher Scientific, USA) and 13 µL of 10X DNase I reaction buffer with MgCl2 (ThermoFisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... All cell lines were grown at 37°C under 5% CO2 in RPMI medium (Gibco BRL, Paisley, UK) supplemented with 10% fetal calf serum (Gibco BRL ...
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bioRxiv - Biochemistry 2024Quote: ... Membranes were blocked with 5% BSA in TBST (Tris buffered saline pH 8.0, 0.1% Tween 20, Thermo Scientific) for 1 hour at room temperature and incubated with primary antibody overnight at 4°C ...
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Recruitment of the m6A/Am demethylase FTO to target RNAs by the telomeric zinc finger protein ZBTB48bioRxiv - Molecular Biology 2024Quote: ... Immunoprecipitation was performed using 5 μg of antibody (either anti-ZBTB48, or anti-GFP antibody (Life Technologies G10362), or anti- FLAG ...
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bioRxiv - Microbiology 2024Quote: ... Gametocyte cultures were seeded from asexual blood stage cultures at 2% parasitaemia and 4% haematocrit in gametocyte medium (RPMI-1640 with 5% human AB+ serum, 0.5% Albumax, 3.7% 100xHT supplement (ThermoFisher), 30 mg/ml L-glutamine ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were allowed to attach overnight prior to transfection with plasmid DNAs (0.5-0.75 μg/well) using Lipofectamine 2000 (2-5 μL/well; Invitrogen). Lipofection was done using small volumes of Opti-MEM (200 μL ...
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bioRxiv - Microbiology 2024Quote: ... mAbs were incubated at 37□ for 5 h with 100 µL papain immobilized on agarose resin (ThermoFisher Scientific) in PBS ...