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Citations for Thermo Fisher :
8051 - 8100 of 10000+ citations for 6H 1 3 Dioxolo 4 5 g 1 benzopyran 6 one 7 8 dihydro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... Samples were heated to 90°C over 1 hr and then slowly cooled overnight to 4 °C (−1°C per 20 minutes) using a Thermocycler (ProFlex PCR System, ThermoFisher). Refolded samples were resuspended through pipetting and combined into a fresh 1.5 mL microfuge tube ...
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bioRxiv - Neuroscience 2022Quote: ... free-floating brain sections were incubated overnight at 4°C with primary anti-FSHR (1:200; catalog # PA5-50963, ThermoFisher), anti-NeuN (1:300 ...
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bioRxiv - Genomics 2022Quote: ... 80% confluent flasks of HEK293FT cells were split 1:4 into DMEM culture medium [10% Fetal Bovine Serum (cat. #10437-028, Gibco), 1% PenStrep (cat ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were incubated overnight at 4°C with either mouse anti-mitochondria antibody (Thermo Fisher Scientific, 1:200, # MA5-12017); mouse anti-nuclei antibody (Merck ...
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bioRxiv - Neuroscience 2022Quote: ... cDNA prepared thus was diluted 1:60 and 4 µL of the diluted cDNA was used for each qPCR reaction with SYBR green (Invitrogen). β-actin was used as the reference endogenous gene for normalization ...
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bioRxiv - Microbiology 2022Quote: ... Cells were washed with PBS, and stained using anti-IgG AF647 (1:600 dilution in MACS, 30 min at 4°C) (ThermoFisher). Cells were then fixed using PFA 4% ...
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bioRxiv - Microbiology 2022Quote: ... then washed three times with PBS and incubated with secondary antibodies (Table 4) and phalloidin (F-actin detection) (1:100) (Invitrogen) for 1 hr at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were incubated in the following secondary antibodies diluted to 1:1000 in ovary block overnight at 4°C: goat anti-rabbit IgG Alexa Fluor 633 (Invitrogen), goat anti-mouse IgG Alexa Fluor 488 (Invitrogen) ...
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bioRxiv - Neuroscience 2022Quote: ... Brains were probed overnight at 4°C using the following primary antibody dilutions: rabbit anti-GFP (1:1000, Invitrogen #A11122), and mouse anti-brp (1:50 ...
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bioRxiv - Cell Biology 2022Quote: ... the sections were labeled overnight at 4 °C with Alexa Fluor 488-labeled wheatgerm agglutinin (1:200; W11261, Thermo Fisher) in combination with a rabbit polyclonal primary antiserum ...
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bioRxiv - Microbiology 2021Quote: ... primary antibody for 16 h at 4 °C and detected with anti-rabbit-Alexa488 (1:200, A11008, Invitrogen, CA, USA) secondary antibodies for 40 min at RT ...
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bioRxiv - Molecular Biology 2020Quote: ... The plate is centrifuged for 1 minute at 1,000 rpm at 4°C and then inserted in the qRT-PCR machine (QuantStudio; Thermofisher scientific) and the run is parametrised ...
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bioRxiv - Cancer Biology 2020Quote: ... organoids were incubated with 2 mM Calcein-AM and 4 mM Ethidium homodimer-1 (LIVE/DEAD assay kit, Molecular Probes) for up to 6h ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated overnight at 4°C with gentle agitation in a chicken antiserum against GFP (1:1500, Invitrogen, A11120). After subsequent 3 x 10 min washes in PBST ...
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bioRxiv - Cancer Biology 2021Quote: ... antibodies were used at 1/200 concentration overnight at 4°C and secondary AF488-conjugated anti-mouse Ig (Life Technologies) at 1/500 for 1 hour at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and incubated overnight in a shaker protected from light at 4°C with respective detection reagents (DyLight 488-conjugated streptavidin at 1:400 dilution, Invitrogen #21832 ...
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bioRxiv - Immunology 2022Quote: ... and incubated for 30 min at 4°C the live/dead fixable aqua dead cell marker (1:1,000 in PBS; Life Technologies) before fixation ...
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bioRxiv - Biochemistry 2020Quote: ... in the same buffer at 4 °C overnight followed by incubation with Alexa Fluor 594 anti-mouse IgG Ab (1:1000, Invitrogen) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2019Quote: Cells were fixed in PKM buffer (10 mM Sodium Phosphate, 140 mM KCl and 1 mM MgCl2, pH 7.2) containing 4% paraformaldehyde (Thermo Fisher) for 10 min at room temperature followed by permeabilization in 0.2% Triton X-100 for 5 – 10 min ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were fixed in PKM buffer (10 mM Sodium Phosphate, 140 mM KCl and 1 mM MgCl2 pH 7.2) containing 4% paraformaldehyde (Thermo Fisher) for 10 min at room temperature followed by permeabilization in 0.2% Triton X-100 for 5 – 10 min ...
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bioRxiv - Pathology 2019Quote: ... Muscle fibres were then incubated at 4°C overnight with rabbit polyclonal antibodies against synaptophysin (Syn, 1/500; ThermoFisher Scientific) and neurofilament 68 kDa (1/500 ...
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bioRxiv - Developmental Biology 2019Quote: ... sections were incubated overnight at 4°C in blocking-permeabilizing solution containing antibodies anti-PAX6 (mouse, 1:1000, Thermo Fisher Scientific ...
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bioRxiv - Physiology 2020Quote: ... EDL from both legs were dissected and maintained in 4°C Krebs–Henseleit buffer supplemented with 1×MEM amino acid mixture (Invitrogen) and 25mM glucose ...
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bioRxiv - Synthetic Biology 2021Quote: ... Quality of RNA in each sample was checked on 1% agarose gel and its concentration measured on a Qubit 4 fluorometer (Invitrogen). The isolated RNA samples were then stored in −80°C freezer.
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bioRxiv - Neuroscience 2020Quote: ... Cells were then incubated with primary antibodies in 10% NGS/NDS in PBST over night at 4°C following 1h incubation at 24°C with appropriate Alexa488/568 coupled secondary antibodies (1:1000, Invitrogen). Cell nuclei were stained with DAPI and final images were acquired using Leica TCS SP8 confocal microscope (Leica Microsystems ...
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bioRxiv - Neuroscience 2020Quote: ... in 3% NGS and 0.5% Triton-X for 44 h at 4°C and then in goat anti-mouse secondary antibody Alexa 488 (1:500; Invitrogen) in 3% NGS and 0.5% Triton-X for 2 h ...
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bioRxiv - Immunology 2021Quote: ... The RBD probe was prepared at a molar ratio of 4 moles of protein to 1 mole of Alexa Fluor 647-labeled streptavidin (Invitrogen). PBMCs from the five participants were thawed and stained for SARS-CoV-2-specific IgG+ memory B cells ...
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bioRxiv - Pathology 2021Quote: ... The cDNA of the fusion chimera termed ace2 1–618-ddc-abd was then inserted into pcDNA3-4 plasmid (Invitrogen) using custom synthesized complementary primers (IDT ...
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bioRxiv - Systems Biology 2020Quote: ... the pellet resulting from ultracentrifugation of pooled fractions 1-4 was re-suspended in 700 μl of TRIzol (Invitrogen 15596026). Samples were then vortexed for 1 minute ...
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bioRxiv - Genetics 2019Quote: ... 1.25×10-4 U/µl DNase I and 1 U/µl DNA polymerase I (both ThermoFisher Scientific, Waltham, MA, USA). The reaction was incubated at 15 °C for 40 min and then purified using an illustra Sephadex G-50 column (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... the media was decanted and cells were incubated for 1 h at 37 °C with Fluo-4 Direct dye (Invitrogen) in FLIPR buffer (1× HBSS ...
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bioRxiv - Cancer Biology 2019Quote: ... Culture media was replenished every 4 days and organoids were passaged by sequential digestion in 1 mg/mL Dispase II (Gibco) followed by TrypLE Express (Gibco ...
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bioRxiv - Genomics 2019Quote: ... 300 nM of each primer and 3.75 μL of cDNA 1:4 diluted on a QuantStudio 12K Flex Real-Time PCR System (Applied Biosystems). To evaluate the efficiency of the RT-qPCR assays ...
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bioRxiv - Genetics 2020Quote: ... Protein extracts were run for 30 min at 200 V on denaturing Bolt 4-12% Bis-Tris Plus Gels in 1× Bolt MOPS SDS buffer (Invitrogen), transferred to a nitrocellulose membrane for 1.5 h at 10 V ...
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bioRxiv - Biochemistry 2021Quote: ... and resuspended in plating medium (Dulbecco’s modified Eagle’s medium (DMEM)/medium 199 [4:1 (v/v)]) containing 15% (v/v) foetal calf serum (FCS; Life Technologies) and 100 U/ml penicillin and streptomycin ...
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bioRxiv - Neuroscience 2021Quote: ... Primary microglial cells were prepared from post-natal day 2-4 pups as previously described and cultured in DMEM containing 1% PSF (ThermoFisher) 10% heat inactivated FBS (ThermoFisher ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were incubated with primary antibodies overnight at 4°C using an antibody against V5 (mouse monoclonal R960-25; 1:1000; Invitrogen), spike (mouse monoclonal GTX632604 ...
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bioRxiv - Biochemistry 2021Quote: ... Glycoproteins were resolved by SDS–PAGE (approx. 1×107 cell equivalents/lane) on NuPAGE bis-Tris 4–12% gradient acrylamide gels (Invitrogen) and transferred to nitrocellulose membrane (Invitrogen) ...
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bioRxiv - Cell Biology 2021Quote: ... For peptide treatment, cells were washed with PBS and treated with peptides (40 μM, 1–4 h) dissolved in OPTI-MEM (Gibco) acidified with 0.15% (v/v ...
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bioRxiv - Cell Biology 2020Quote: ... The embryos were washed a further three times in PBTw (PBS + 0.1% Tween) and incubated overnight whilst rotating at 4°C in 0.005 U/µl Alexa Fluor 594 phalloidin (Invitrogen, #A12381) in PBTw ...
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bioRxiv - Molecular Biology 2021Quote: ... N2a cells were transfected with pSpCas9(BB)-Puro empty vector or vectors expressing each of the three gRNAs targeting the eIF4E3 exons (meIF4E3 gRNA exon 1, exon 2, exon 4) using Lipofectamine 2000 (Invitrogen). pSpCas9(BB)-Puro was a generous gift from Dr ...
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bioRxiv - Plant Biology 2020Quote: ... Quality and quantity of bulked DNA was checked by running a DNA sample on an agarose gel (1%) and final DNA concentration determined using the Quibit-4 Fluorometer (dsDNA BR assay, Q32850, Thermofisher).
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bioRxiv - Cancer Biology 2020Quote: ... All cell lines were maintained in DMEM (25 mM Glucose, 4 mM Glutamine, 1 mM Sodium Pyruvate) supplemented with 10% Fetal Bovine Serum (FBS, Gibco) and 50 units/mL Penicillin/Streptomycin (Gibco ...
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bioRxiv - Immunology 2022Quote: Bacteria in PBS were first stained for 1 h at 37°C with 4 μM Oregon Green 488-X succinimidyl ester (Invitrogen) followed by 20 μg/ml CypHer5E (Cytiva ...
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bioRxiv - Biochemistry 2022Quote: ... C6 cells were grown and maintained in High Glucose DMEM (25 mM glucose, 1 mM pyruvate, 4 mM glutamine; #12800017, Gibco) supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin ...
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bioRxiv - Cell Biology 2022Quote: ... Skeletal muscle were stained with Myosin 4 monoclonal antibody (MF20, MyHC-2) (1:200; Thermo Fisher Scientific, Rockford, IL, USA) and Laminin gamma 1 Antibody (A5 ...
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The MuSK-BMP pathway maintains myofiber size in slow muscle through regulation of Akt-mTOR signalingbioRxiv - Cell Biology 2022Quote: ... Serum was diluted 1:1000 in 1/10 block overnight at 4°C and detected using goat anti-human IgG Alexa Fluor 555 (Invitrogen) diluted 1:1000 and counterstained with AlexaFluor488-conjugated α-bungarotoxin (Thermo Fisher ...
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bioRxiv - Neuroscience 2022Quote: ... the slices were incubated with the primary antibodies at 4 °C for 48 h (GFP, chicken, abcam, 1:2000; GFAP, rat, Invitrogen, 1:1000 ...
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bioRxiv - Microbiology 2022Quote: ... Total lipids were extracted by resuspending washed cell pellets in 1 mL HPLC grade methanol (A454-4, Thermo Fisher Scientific), transferring to a glass vial with PFTE cap ...
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bioRxiv - Neuroscience 2022Quote: ... and spinal cord medium (MEM with Glutamax [Gibco] supplemented with 4 mg/ml Albumax [Gibco], 1 mM pyruvate [Sigma], 100 units/ml Penicillin and 100 μg/ml Streptomycin [Gibco]), as previously described (Dumoulin et al. ...