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Citations for Thermo Fisher :
8151 - 8200 of 10000+ citations for 6H 1 3 Dioxolo 4 5 g 1 benzopyran 6 one 7 8 dihydro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... diluted in PBS-T overnight at 4°C and detected with 1:2000 HRP-conjugated goat anti-chicken secondary antibody (#A16054, Invitrogen) diluted in PBS-T during 1 hour at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were incubated overnight at 4°C with the following primary antibodies: TMEM163 (Thermofisher, cat # PA5-114329; 1:100), CD83 (Biolegend ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were washed with assay buffer (PBS containing 0.2% BSA and 1 mM CaCl2) and resuspended in assay buffer with 2 μM Fluo-4-AM (Life Technologies). Fluo-4-AM was prepared as a 1 mM stock in DMSO ...
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bioRxiv - Synthetic Biology 2023Quote: ... the membrane was incubated overnight at 4°C with primary antibody solution (1:1000 dilution for anti-TSG101 [Abcam, ab30871], anti-Calnexin [ThermoFisher, PA5-19169] and anti-Syntenin-1 [Origene ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were spun at 100,000xg for 1 hour at 4°C using Sorvall Discovery MX120SE Ultracentrifuge (Thermo Scientific; Waltham; MA) to isolate the cytosolic fraction ...
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RNA-binding protein YBX1 promotes Type H vessels dependent bone formation in an m5C-dependent mannerbioRxiv - Molecular Biology 2023Quote: ... the cells were counted and incubated for 45 min at 4°C with EMCN antibody (#2114518, Invitrogen, USA, 1:100) and CD31 antibody (#FAB6874G ...
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bioRxiv - Neuroscience 2023Quote: ... Thick slices were then incubated overnight at 4°C with streptavidin coupled to Alexa-568 (1/500, Invitrogen, S11226, RRID:AB_2315774) diluted in PBS containing 0.3% Triton X100 and 1% BSA ...
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bioRxiv - Neuroscience 2023Quote: ... the immediate adjacent sections (4-µm thickness) were stained against phospho-tau AT8 (pS202/pT205, 1:1,000 dilution, Thermo Scientific) using the Autostainer Link 48 (Dako) ...
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bioRxiv - Neuroscience 2023Quote: ... cells were resuspended in the corresponding volume of M10 medium (1 mL per 4 hemispheres) composed of DMEM/F12 (Gibco) supplemented with 10 % FBS (GE Healthcare Hyclone) ...
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bioRxiv - Neuroscience 2023Quote: ... The lysates were incubated overnight at 4°C with a 1:100 dilution of anti-RyR2 antibody (C3-33, Invitrogen). Following the overnight incubation ...
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bioRxiv - Biochemistry 2024Quote: ... The sample was dialysed in 1 L of purification buffer at 4°C using Snakeskin Dialysis Tubing (3.5 k MWCO, Thermo Fisher), followed by an overnight dialysis under the same conditions.
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bioRxiv - Physiology 2024Quote: ... sections were incubated overnight at 4°C in primary antibody solution containing 10% goat serum and antibodies against laminin β1 (1:100; MA5-14657, Invitrogen) or laminin β1γ1 (1:200 ...
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bioRxiv - Microbiology 2023Quote: ... The epidermis was peeled off and discarded and the dermis was minced into small pieces and digested at 37°C for 1 h following 4°C for overnight using collagenase IV (17104019, Gibco). The resulting cell suspension was filtered through a 70 μm cell strainer (BD Falcon) ...
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bioRxiv - Molecular Biology 2023Quote: ... for at least 1hr and incubated overnight with primary antibodies at 4°C (anti-METTL3, ThermoFisher # 15073-1-AP; anti-RNGTT, ThermoFisher # 12430-1-AP ...
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bioRxiv - Neuroscience 2023Quote: ... and stained by sequential incubation with primary antibodies diluted with PBS at 4°C overnight and with Alexa Fluor 488- or Alexa Fluor 555-conjugated secondary antibodies (1:450; ThermoFisher) or NeuroTrace500/525 (1:250 ...
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bioRxiv - Biophysics 2023Quote: HEK-293 cells overexpressing GluA2 or GluA2-TARPγ2 receptors were labeled with 1:4 ratio of maleimide derivatives of Alexa 555 (donor) and Alexa 647 (acceptor) fluorophores (Invitrogen) in extracellular buffer (135 mM NaCl ...
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bioRxiv - Cancer Biology 2023Quote: ... The cells were then co-transfected with the Puro TK targeting vector and Ctnnb1 sgRNA1-4 (Supplementary table 1) using lipofectamine 2000 (Invitrogen) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... membranes were incubated and cut depending on their molecular weight (KDa) and incubated with primary antibodies overnight at 4°C in a shaker (Cofilin Total 1:500 Invitrogen #MA517275 ...
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bioRxiv - Biochemistry 2023Quote: ... at a final concentration 2.5 μg/mL for 1 h at 4 °C followed by Concanavalin A conjugated with Alexa Fluor 350 (Thermo Scientific) at final concentration 0.5 mg/mL for 3 h at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... To 32 μl of the cleared cell extract 4 μl of FastAP phosphatase (1 U/μl, ThermoFisher Scientific, Dreieich, Germany) and 4 μl of 10-times FastAP buffer were added ...
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bioRxiv - Neuroscience 2023Quote: ... for 24 hours at 4°C followed by incubation with a biotinylated goat anti-mouse (1:200, ThermoFisher Scientific A28181) antibody for two hours at room temperature and then a streptavidin conjugated Alexa-Fluor 488 (1:200 ...
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bioRxiv - Developmental Biology 2023Quote: ... Membranes were blocked for 2hrs at RT in 5% milk powder and then incubated overnight (o/n) at 4°C with primary rabbit anti-GFP antibody (1:1,000; Molecular Probes). Membranes were washed 3x for 10min in 1X TBST ...
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bioRxiv - Cell Biology 2023Quote: Plasmids were transiently transfected to HEK293T cells for transgene expression using FuGENE (4 μL FuGENE per 1 μg of DNA) in 100 μL of Opti-MEM (Gibco) per well of a six-well plate (2 μg DNA total per well) ...
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bioRxiv - Cancer Biology 2023Quote: ... and then incubated overnight at 4°C in a humidified chamber with anti-γ-H2AX (1:500, Invitrogen MA5-38225), anti-perilipin-2 (1:200 ...
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bioRxiv - Plant Biology 2024Quote: All fragments including the CENPC-k and CENPC motifs with and without DNA-binding regions (Supplementary Table 1-4) were amplified from the αKNL2 and CENP-C cDNA clones in pDONR221 (Invitrogen) vector using primers listed in Supplementary Table 5 ...
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bioRxiv - Developmental Biology 2024Quote: ... Slices were incubated for 48h at 4°C with primary antibodies anti-GFP (A11122, Invitrogen, Thermo Fisher scientific, 1:500), anti-Robo3 human (AF3076 ...
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bioRxiv - Developmental Biology 2024Quote: ... for two nights at 4°C and then with Alexa Fluor 555-conjugated Donkey anti-rat secondary antibody (1:50; Cat. No. A11077, Invitrogen). Light-sheet images were captured by LiTone XL Light-sheet Microscope (Light Innovation Technology Limited ...
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bioRxiv - Cell Biology 2024Quote: ... overnight at 4°C followed by corresponding Alexa Fluor 488- and Alexa Fluor 594-conjugated secondary antibodies (1:500; Invitrogen) or anti-αSMA-Cy3 antibody (Sigma Aldrich ...
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bioRxiv - Biophysics 2024Quote: ... samples corresponding to 1 µg Chl were separated on a 4-12% NuPAGE Bis-Tris gel (Invitrogen, Thermo Fisher Scientific) following the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2024Quote: ... Samples were incubated at 95 °C for 10 min then centrifuged at 14,000 xg for 1 min before loading on Novex 4-20% Tris-Glycine gel (Invitrogen, XP04205). After transfer to PVDF-FL (Merck Life Sciences ...
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bioRxiv - Developmental Biology 2024Quote: ... Slices were incubated for 48h at 4°C with primary antibodies anti-GFP (A11122, Invitrogen, Thermo Fisher scientific, 1:500), anti-Robo3 human (AF3076 ...
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bioRxiv - Developmental Biology 2024Quote: ... incubated 1 hour in blocking solution and incubated at 4 °C overnight with secondary antibodies conjugated with AlexaFluor (Molecular probes) diluted in blocking solution ...
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bioRxiv - Cell Biology 2024Quote: ... Sample protein content was determined with the Pierce detection assay and a maximum amount of proteins (2.3-4 mg) was loaded on 1 mg of Dynabeads M-280 Streptavidin (ThermoFisher Scientific), previously equilibrated with RIPA buffer ...
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bioRxiv - Biophysics 2024Quote: ... samples corresponding to 1 µg Chl were separated on a 4-12% NuPAGE Bis-Tris gel (Invitrogen, Thermo Fisher Scientific) following the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2024Quote: ... Larvae were thoroughly washed in PBS-Tx and incubated overnight at +4°C in secondary antibody (1:400 goat anti-chicken Alexa488 A-11039, ThermoFisher) in blocking buffer ...
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bioRxiv - Neuroscience 2024Quote: ... the sections were incubated in the same solution overnight at 4 °C with the primary antibodies anti-GFP (1:1000, Invitrogen) and anti-phospho-S6 Ribosomal protein (Ser236/236 ...
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bioRxiv - Developmental Biology 2024Quote: ... for 2 hrs at RT and incubated overnight at 4°C in primary antibody solution (1:100 rabbit anti-GFP (ThermoFisher) in blocking reagent) ...
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bioRxiv - Systems Biology 2024Quote: ... or 5 μg cleared lysate from cardiomyocytes were purified by a brief 1 cm SDS-PAGE run (Nu-PAGE minigel 4-12%, Invitrogen). After Coomassie staining ...
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bioRxiv - Molecular Biology 2024Quote: ASOs were removed from 4 µl of DMS-modified RNA in 10 µl containing 1 µl of TURBO DNase (Invitrogen) and 1X TURBO DNase Buffer (Invitrogen) ...
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bioRxiv - Immunology 2024Quote: ... The fibroblasts were incubated with primary antibody overnight at 4°C and then stained with the secondary antibody (all 1:1000, Alexa Fluor; Invitrogen) for 1 h ...
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bioRxiv - Immunology 2024Quote: ... and THP-1 cells were incubated with GZMK (50 nM) or C1s (50 nM) for 1 h on ice at 4 °C in HBSS with Ca2+ and Mg2+ (Gibco) containing 10 mM HEPES and 0.5% BSA (Millipore Sigma) ...
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bioRxiv - Cancer Biology 2024Quote: ... and then were incubated overnight at 4 °C with Alexa Fluor 488-labeled secondary antibody (Fisher Scientific, A32723, 1:200), and mounted with DAPI (Vector Laboratories) ...
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LIR-dependent LMX1A/LMX1B autophagy crosstalk shapes human midbrain dopaminergic neuronal resiliencebioRxiv - Cell Biology 2019Quote: ... NAS2 were maintained in Essential 8 TM Medium (E8) supplemented with Essential 8 Supplement (Thermo Scientific) and RevitaCell (1/100 ...
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bioRxiv - Cancer Biology 2024Quote: 8 •104 cells grown in 8-well Lab-TEK II glass chamber slides overnight (177380, Thermofisher). Cells were fixed with 4% PFA in PBS for 10 minutes ...
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bioRxiv - Developmental Biology 2024Quote: ... RRID:CVCL_AH05) were cultured in Essential 8 or Essential 8 FLEX media (Life Technologies # A1517001 and # A2858501) on vitronection (Thermo Fisher #15134499 ...
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bioRxiv - Plant Biology 2020Quote: ... QuantStudio 6 (Thermo Scientific) and specific primers ...
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bioRxiv - Plant Biology 2020Quote: ... 6-mercaptopurine (Fisher Scientific), 5-fluorouracil (Fisher Scientific) ...
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bioRxiv - Neuroscience 2021Quote: ... Il-6 (ThermoFisher, Mm00446190_m1), Nlrp3 (ThermoFisher ...
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bioRxiv - Neuroscience 2019Quote: ... Fugene 6 from Invitrogen.
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bioRxiv - Cell Biology 2019Quote: ... Sas-6 (Thermofisher, Switzerland) – GCACGUUAAUCAGCUACAAdTdT (Leidel et al. ...