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Citations for Thermo Fisher :
7901 - 7950 of 10000+ citations for 6H 1 3 Dioxolo 4 5 g 1 benzopyran 6 one 7 8 dihydro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Coverslips were washed with 5% FBS and stained with AlexaFluor-488-conjugated goat anti-rat IgG (Invitrogen, 1:500) for 1 h at RT ...
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bioRxiv - Physiology 2021Quote: ... were plated in plates 1 day before transfection with a total of 2 μg of DNA (1 μg mouse pCMV6-Tmem27-Flag and 1 μg empty vector or pCDNA3.1-BACE2-myc) and 5 μL Lipofectamine 2000 (Invitrogen).
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bioRxiv - Cancer Biology 2020Quote: ... samples were blocked in PBS +5% BSA +0.3% Triton X-100 in PBS and stained using an AlexaFluor488-labeled ZO-1 (#339188, Invitrogen) or ROR2 (#FAB20641G ...
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bioRxiv - Genetics 2020Quote: ... qPCR were carried out on the generated cDNA (diluted at 1/5) using PowerUp SYBR master mix (Thermofisher Scientific) in a final volume of 10 μl and 40 cycles of amplification on QuantStudio3 Real-Time PCR System (ThermoFisher) ...
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bioRxiv - Immunology 2021Quote: ... Slices were maintained in a humidified incubator with 5% CO2 at 37°C with 1 ml MEM (Gibco, 31095209) containing 20% (v/v ...
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bioRxiv - Immunology 2021Quote: ... Then, 5 x106 cells were washed in PBS (HyClone Laboratories, Logan, UT, USA) containing 1 % BSA (Thermo Fisher Scientific) and stained in 100 μL PBS and BSA 1 %-containing Antibody cocktail ...
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bioRxiv - Immunology 2020Quote: ... Plates were blocked for 1 hr at room temperature with 200 μl 5% Blocker™ BLOTTO (Thermo Scientific, #37530) and washed three times with 200 μl PBS-T ...
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bioRxiv - Developmental Biology 2021Quote: ... resuspendend in 1 mL M9 before 5 µL of DiI stock solution (2 mg/mL DiI (Molecular Probes, #D282) in dimethyl formamide ...
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bioRxiv - Developmental Biology 2022Quote: ... the samples were washed 3x for 5 minutes in PBT and incubated in corresponding Alexa Fluor (1:500, Invitrogen) secondary antibodies for 2 hours ...
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bioRxiv - Cancer Biology 2022Quote: ... were diluted 1:5000 in 5 % BSA in TBST or in blocking buffer (Cat. no. 37538, Thermo Fisher Scientific) in PBS (1:1 ...
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bioRxiv - Cell Biology 2022Quote: ... cells were permeabilized with saponin (0.2%) for 5 min and incubated with DAPI (1 μg/mL) (ThermoFisher, catalog #: D1306) for 3 min to stain the nucleus ...
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bioRxiv - Neuroscience 2022Quote: ... Eyes were intravitreally injected using a glass micropipette with 1-5 μl of 0.2% cholera toxin subunit B (CTB) conjugated to Alexa Fluor 488 or 555 (Invitrogen) diluted in 1% DMSO ...
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bioRxiv - Molecular Biology 2022Quote: 1-5 × 107 parasites were harvested by centrifugation and RNA was extracted from cells using Trizol (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... Confluent TRF cells were infected with 1 MOI FL-RhCMV in low serum (5%) medium in T75 flasks (ThermoFisher). Mock infection was performed in parallel ...
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bioRxiv - Immunology 2024Quote: ... 20.000 BMDCs were co-cultured with purified T cells in a 1:5 DC:T cell ratio in sterile tissue culture medium (TCM; RPMI (GIBCO) containing 10% fetal calf serum (Bodinco) ...
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were washed with PBS-0.1%Tríton-X three times (5 minutes each) and incubated with the secondary antibodies (1:2000; Alexa-Fluor conjugated; Invitrogen), at RT for 1h ...
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bioRxiv - Genetics 2024Quote: ... Tissues were rehydrated with PBS and blocked for 1 hour at room temperature with 5% normal goat serum ((Gibco) source ...
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bioRxiv - Biochemistry 2024Quote: To perform DSF, 5 µM protein was mixed with substrate (0, 1, or 15 mM) and 2.5x SYPRO Orange dye (ThermoFisher), and made up to a total volume of 50 µl with DSF buffer (50 mM Tris pH 8 ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were incubated for 5 hrs before the media was changed to media + 1% Calcein AM stain (Thermo Fisher) and incubated for a further hr ...
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bioRxiv - Microbiology 2023Quote: ... Secondary antibodies were prepared in 5% goat serum in blocking buffer: goat anti-mouse 594 (AlexaFluor Invitrogen, 1:2000) and goat anti-rabbit 488 (AlexaFluor Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... Background staining was blocked in 5% serum and incubated with either an anti-MHCII (1:500, M5/114.15.2; Thermo Fisher) or a pSer129 (1:5000 ...
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bioRxiv - Neuroscience 2023Quote: ... Neurons were resuspended for a final concentration of 50,000 cells per 5 μL in Neuronal media supplemented with 1% anti-anti (GIBCO), 5% KSR ...
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bioRxiv - Immunology 2023Quote: All single cell suspensions were prepared in Hank’s buffer (1×HBSS, 5% FCS, 10mM HEPES; all ThermoFisher, Life Technologies). For this ...
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bioRxiv - Immunology 2023Quote: All single cell suspensions were prepared in Hank’s buffer (1×HBSS, 5% FCS, 10mM HEPES; all ThermoFisher, Life Technologies). For this ...
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bioRxiv - Cell Biology 2023Quote: ... the samples were incubated with DAPI (1/25,000 in PBS) for 5 min and mounted with Prolong Diamond antifade mounting medium (Invitrogen). The samples were imaged and analyzed with C-Plan Apochromat 63x/1.4 Oil DIC M27 objective under confocal microscope (LSM780NLO ...
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bioRxiv - Cell Biology 2023Quote: ... approximately 1 μg of peptides were loaded on a C18 precolumn (PepMap100, 5 μm, 300 Å, Thermo Fisher Scientific) and separated over a C18 column (50 cm × 75 μm ...
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Autoimmune inflammation triggers aberrant astrocytic calcium signaling to impair synaptic plasticitybioRxiv - Neuroscience 2023Quote: ... sections were washed three times for 5 min in PBS (0.1 M, pH 7.4) and incubated with rabbit anti-GFP (1:500; Invitrogen, A11122) and chicken anti-GFAP (1:500 ...
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bioRxiv - Cell Biology 2023Quote: ... 5×107 ES cells engineered with T7-dTAG-TAF1 were fixed with 1% formaldehyde (methanol-free, Thermo Fisher Scientific) for 10 min at 25C under constant gentle rotation ...
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bioRxiv - Biochemistry 2022Quote: ... After membrane permeabilization with 1% Triton X-100/D’PBS for 5 min cells were incubated for 30 min in the primary antibody aPMP70 (rabbit, 1:500, Invitrogen) in D’PBS supplemented with 1% BSA ...
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bioRxiv - Cell Biology 2022Quote: ... SPD-5 and GIP-1 cDNAs was amplified by PCR and cloned into the entry vector pDONR201 (Thermo Fisher). Thereafter ...
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bioRxiv - Cell Biology 2023Quote: ... 300-1500 ng DNA was incubated with 1-5 μl Lipofectamine 2000 in 150 μl Optimem (Thermo Fisher, 31985047) for 10 minutes at room temperature and then given to cells ...
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bioRxiv - Cancer Biology 2023Quote: ... On day 5 the culture medium was replaced with DMEM/F12 containing 1% N2 (v/v, Gibco, Cat#17502048), 1% GlutaMAX (v/v) ...
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bioRxiv - Genomics 2023Quote: ... 5 μL of each restriction digest reaction was combined with 1 μL 6X TriTrack DNA Loading Dye (Thermo Fisher) and run on a 2% agarose gel pre-stained with ethidium bromide ...
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bioRxiv - Biophysics 2023Quote: ... immunostained cells were exposed for 5 min to 1 mg/100 ml Hoechst 33342 (Thermo Fisher Scientific, Waltham, Massachusetts) in water.
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bioRxiv - Bioengineering 2023Quote: ... in PBS for 1 hour at room temperature and subsequently blocked with 5% bovine serum albumin (ThermoFisher Scientific 37525) and 10% donkey serum (Lampire 7332100 ...
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bioRxiv - Immunology 2023Quote: ... Confluent TeloRF cells were infected with 1 MOI UCD52 RhCMV in low serum (5%) medium in T75 flasks (ThermoFisher). Mock infection was performed in parallel ...
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bioRxiv - Biophysics 2023Quote: ... HeLa and MDCK cells were cultured at 5% CO2 and 37°C in Dulbecco’s modified Eagle medium (DMEM, Capricorn) with 1% Penicillin Streptomycin (Gibco) and 10% fetal bovine serum (FBS ...
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bioRxiv - Synthetic Biology 2023Quote: ... 3 nM of fluorescently labeled and biotinylated DNA (or 2x1.5 nM with experiments for the intermolecular cases) were bound to 5 nM Streptavidin in PBS and with 1:1800 Tetraspeck beads (0.1 µm, ThermoFisher). After ∼10 min incubation time ...
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bioRxiv - Molecular Biology 2023Quote: ... the membrane was incubated with secondary goat anti-rabbit antibodies (dilution 1:1000 in TBST/5% milk; Invitrogen, 32460) for 1 hour at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Tissue was washed for 5 min on ice with 1× Hanks’ balanced salt solution (HBSS, Gibco, Carlsbad, CA, USA) and cut into small pieces ...
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bioRxiv - Neuroscience 2023Quote: ... Secondary antibodies were used at a ratio of 1:500 in 5% GS/1% BSA/0.2% PBST (anti-rabbit-Alexa-Fluor-555, Invitrogen A27039; anti-mouse-Alexa-Fluor-647, Invitrogen A21235 ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5% Fetal Bovine Serum (BioWest, cat# S1620) and 1% Penicillin/Streptomycin (Fisher Scientific, cat#15-140-122) unless otherwise noted ...
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bioRxiv - Cancer Biology 2023Quote: Single cell suspensions from dissociated larvae in 5 mL FACS tubes were stained with 1:1000 DAPI (Thermofisher, #62248) to indicate cell viability and FACS was carried out at the QMRI Flow facility using a BD 5 Laser LSR Fortessa cell analyser ...
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bioRxiv - Cancer Biology 2023Quote: Approximately 1 × 107 cells were resuspended in 0.5 ml cold TNE buffer (25 mMTris-HCl, pH 7.5, 150 mM NaCl, 5 mM EDTA, and 1% Triton X-100; Surfact-Amps, ThermoFisher) with a tablet of protease inhibitors (Roche) ...
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bioRxiv - Biophysics 2023Quote: ... About 5 µl of a 1 µg/µl sample was injected into a QExactive-Orbitrap mass spectrometer (Thermo Scientific) and a data-independent acquisition (DIA ...
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bioRxiv - Molecular Biology 2023Quote: ... The membranes were blocked with 5% BSA and probed against P-S19-TH (#PA5-104765, ThermoFisher Scientific; 1:1000), P-S40-TH (#p1580-40 ...
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bioRxiv - Microbiology 2024Quote: ... synchronized iRBCs at the trophozoite stage (5-10% parasitemia, 1% hematocrit) were resuspended in binding medium (RPMI 1640 medium, 2% glucose, pH 7.2; Gibco, Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... resuspended in 1 ml of 1xPBS containing 5 µg/ml of the red-fluorescent membrane dye FM5-95 (Invitrogen) and then incubated in the dark at room temperature for 10 min followed by two wash steps with 1x PBS ...
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bioRxiv - Immunology 2024Quote: 2×106 THP-1 cells were centrifuged for 5 min at 1200 rpm and washed in 1X PBS (Gibco). They were then transfected by nucleofection with 3 μg of plasmid DNA in 100 μl of Cell Line Nucleofector Solution V (Lonza ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 5-LOX was stained with Alexa Fluor™ 555 goat anti-mouse IgG (1:500, # A21422, Lot: 10143952; Invitrogen), and perilipin-2 was stained with Alexa Fluor™ 488 goat anti-rabbit IgG (1:500 ...