Labshake search
Citations for Thermo Fisher :
7951 - 8000 of 10000+ citations for 6H 1 3 Dioxolo 4 5 g 1 benzopyran 6 one 7 8 dihydro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... The lentivirus vectors were produced in HEK293FT cells by the co-transfection of plasmids pMD2.G and pCMVR8.74 with the pLKO.1-sh PKM2 using Lipofectamine 2000 (Thermo Fisher Scientific, Waltham, MA, USA). To produce H1299 shPKM2 cells ...
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bioRxiv - Immunology 2020Quote: ... 1.5 g/L sodium bicarbonate supplemented with 10% heat-inactivated fetal bovine serum and 1% streptomycin and penicillin (Life Technologies, Grand Island, NY, USA). Human primary bronchial epithelial cells were purchased from PromoCell (Heidelberg ...
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bioRxiv - Neuroscience 2020Quote: Samples (5–10 µg protein) were typically run on 4–12% Bis-Tris gels (Life Technologies; WG1403BOX10) using MOPS buffer (NP0001 ...
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bioRxiv - Cell Biology 2021Quote: ... boiled for 5-10 min before the loading on 4-12 % Bis-Tris gels (Nu-PAGE, Invitrogen) and then transferred onto nitrocellulose membranes (GE Healthcare) ...
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bioRxiv - Cell Biology 2021Quote: ... Exon 4 and Exon 5 were amplified (Table M1) and cloned using pJET1.2 cloning kit (Thermo Fisher) before sequencing.
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bioRxiv - Molecular Biology 2021Quote: ... L-glutamine (5 mg/ml) in the presence 4 μg/ml Blasticidin S HCl (A11139-03, Invitrogen) and 35 μg/ml Zeozin (R250-01 ...
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bioRxiv - Developmental Biology 2022Quote: ... at 95°C for 5 min and separated using NuPAGE 4%–12% Bis-Tris Protein Gel (Invitrogen) in NuPAGE MES SDS Running Buffer (Invitrogen) ...
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bioRxiv - Immunology 2020Quote: ... centrifuged at 14,000 RPM for 5 min at 4°C and resuspended in UltraPure H2O (Ambion, UK). Purified transcripts were capped using the ScriptCap™ Cap 1 Capping System Kit (CellScript ...
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bioRxiv - Immunology 2020Quote: ... Lipid droplets and nuclei were stained with 0.2% Triton-X100/PBS containing 4,4-difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene (BODIPY 493/503, 5 μg/ml; Thermo Fisher)88 ...
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bioRxiv - Microbiology 2022Quote: ... blotted for 4-5 s and plunge-frozen into liquid ethane in a Vitrobot Mark IV (ThermoFisher), while the blotting chamber was maintained at 100% humidity at 10 ºC ...
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bioRxiv - Immunology 2022Quote: ... centrifugation (table top max speed; 5 mins) before they were run on 4-12% polyacrylamide gels (Invitrogen). Proteins were transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Cell Biology 2022Quote: ... the equivalent of 5-10 adult worms was loaded into 4-12% NuPAGE Bis-Tris gels (Invitrogen). Proteins were transferred overnight to nitrocellulose membranes ...
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bioRxiv - Biochemistry 2022Quote: ... with a 5 eV slit width and a Falcon 4 or Falcon 4i direct electron detector (ThermoFisher). Images were collected at a pixel size of 0.745 Å/pix or 0.951Å/pix with a cumulative dose of 40e−/Å2 for untilted ...
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bioRxiv - Molecular Biology 2022Quote: Arabidopsis seedlings of 5 days after germination (DAG) were fixed in 4% methanol-free formaldehyde (Thermo Scientific) diluted in nuclease free 1 x PBS (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... After 4-5 h bacteria were collected by centrifugation (5000 rpm, SLA 3000 rotor, ThermoFisher Cat#07149) for 15 minutes and resuspended by gentle trituration in ice-cold 50 ml of TBS Buffer (150 mM NaCl ...
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bioRxiv - Molecular Biology 2022Quote: ... heated for 5 minutes at 95C and separated on 4-12% NuPAGE® Bis-Tris gel (Invitrogen) by electrophoresis and subsequently transferred onto Immobilon-P™ PVDF membranes of 0.45 μm pore size (Millipore® ...
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bioRxiv - Cancer Biology 2023Quote: ... Pancreatic tissues were incubated for 24 hours at 4°C in ≥5 volumes of RNAlater solution (ThermoFisher) to preserve RNA integrity ...
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bioRxiv - Developmental Biology 2023Quote: ... at 95°C for 5 min and separated using NuPAGE 4%–12% Bis-Tris Protein Gel (Invitrogen) in NuPAGE MES SDS Running Buffer (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were boiled for 5 min prior to loading onto NuPAGE 4-12% Bis-Tris gels (Invitrogen). After washing with water and then with 5% glycerol ...
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bioRxiv - Cancer Biology 2023Quote: ... then 5 × 107 cells were lysed in 4 ml Pierce IP Lysis Buffer (87788; Thermo Fisher Scientific) for 20 minutes on ice ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were boiled for 5 minutes before loading duplicate 4-12% Bis-Tris SDS-PAGE gels (ThermoFisher) and running gel electrophoresis (1 hour ...
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bioRxiv - Microbiology 2022Quote: ... Cell lysates were then incubated overnight at 4°C with pan-flavivirus anti-E antibody and protein G Sepharose beads (Thermo Fisher Scientific). Beads were washed three times and eluted with 2× SDS loading buffer before Western blot analysis.
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bioRxiv - Cell Biology 2019Quote: ... Lysates were then cleared by centrifugation for 10 minutes at 12,000 x g at 4°C in a Sorval SS-34 fixed-angle rotor (Thermo Scientific, Waltham, MA). The supernatant was then incubated with glutathione-agarose (Sigma Aldrich ...
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bioRxiv - Immunology 2019Quote: ... Spirochetes were centrifuged at 3300 × g for 20 minutes at 4°C and resuspended in either BSK-II for in vivo experiments or DMEM (Gibco, 15630-080) supplemented with sodium pyruvate (Gibco ...
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bioRxiv - Cell Biology 2019Quote: ... The lysate was centrifuged at (21000 g) for 15 min at 4°C and protein concentration determined (Pierce™ 660nm Protein Assay Reagent, Thermo Scientific). 8.5 μg lysate was kept for western blot analysis and 1.6-1.8 mg total protein was added to 250 μl washed high capacity neutravidin beads (Life Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... as per the manufacturer’s instructions and incubated with 10 μg primary antibodies overnight at 4°C followed by 90 minutes incubation with 35 μl protein A/G agarose plus (50% slurry) (ThermoFisher Scientific 20421). Immune pellets were washed 5 times in PBS containing 1% Triton-X100 and analyzed by SDS-PAGE followed by either immunoblotting or mass-spectrometry (LC/MS-MS) ...
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bioRxiv - Cancer Biology 2021Quote: ... Lysates were centrifuged for 5 min at 13 000 × g at 4°C and the concentration of protein was measured using a bicinchoninic acid assay (Thermo Fisher Scientific). Protein lysates were fractionated by SDS-PAGE and transferred onto PVDF membranes ...
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bioRxiv - Biochemistry 2020Quote: ... The chromatin was incubated with antibodies at 4 °C overnight and then added to 30 μl of protein G-Dyna beads (Thermo Fisher Scientific) for an additional 2 hours with constant rotation ...
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bioRxiv - Neuroscience 2021Quote: ... Protein concentrations were determined following centrifugation of the lysate at 16,000 x g at 4 °C for 20 minutes using the using a Pierce™ Coomassie (Bradford) Protein Assay Kit (23200, Thermo Scientific) with bovine serum albumin as the standard.
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bioRxiv - Microbiology 2020Quote: ... lysates from 106 cells were immunoprecipitated using 4 µg anti-SLC35A1 antibody and Protein G magnetic Dynabeads (Thermo Scientific, 10-003-D) overnight at 4C prior to immunoblotting to enhance signal and specificity of signal.
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The pan-cancer lncRNA PLANE regulates an alternative splicing program to promote cancer pathogenesisbioRxiv - Cancer Biology 2020Quote: ... 4 μg antisense / sense biotin-labelled probes were incubated with lysates at 4 °C overnight before rotating with streptavidin beads (ThermoFisher Scientific, #20349) for additional 2 hr ...
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bioRxiv - Cell Biology 2020Quote: ... 100-120 µg of total protein were run on Invitrogen NuPAGE 4-12% precast Bis-Tris polyacrylamide gels for SDS-Page (Thermo Fisher Scientific) and then transferred onto nitrocellulose membranes for Western Blotting ...
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bioRxiv - Cancer Biology 2022Quote: ... overnight on an end-to-end rotator at 4°C followed by a 2.5-hr incubation with protein G Dynabeads (Thermo Fisher Scientific #10003D). Samples were then sequentially washed with TSE (0.1% SDS ...
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bioRxiv - Microbiology 2021Quote: ... Sorted cells were centrifuged for 10 minutes at 1,000×g and pelleted on a glass slide using a cytospin 4 cytocentrifuge (Thermo Fisher scientific, USA). Glass slides were air dried ...
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bioRxiv - Cell Biology 2021Quote: ... 4 μg of anti-HECTD1 antibody or normal Rabbit IgG was coupled to Dynabeads® magnetic beads protein G (ThermoFisher Scientific, #10003D) for 1 hr at RT ...
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bioRxiv - Biochemistry 2020Quote: ... The chromatin was incubated with antibodies at 4 °C overnight and then added to 30 μl of protein G-Dyna beads (Thermo Fisher Scientific) for an additional 2 hours with constant rotation ...
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bioRxiv - Microbiology 2020Quote: ... Lysates were clarified by centrifugation (12,000 g for 30 min at 4 °C) and protein concentrations were determined using a BCA protein assay kit (Thermo Fisher Scientific). SOD activity in whole cell extracts was measured using the EnzyChrome superoxide dismutase assay kit (BioAssay Systems ...
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bioRxiv - Microbiology 2020Quote: ... They were then spun down at 15,000 g for 45 minutes at 4°C and the supernatant was passed over a nickel resin column (Thermo Fisher Scientific, # PI88222) and eluted using buffer B (30mM K2HPO4 ...
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bioRxiv - Neuroscience 2022Quote: ... Protein lysates were pre-cleared 1h at 4°C on a rotating wheel with 50μl of previously washed Dynabeads Protein G (Invitrogen, Thermo Fisher Scientific), to avoid aspecific bindings ...
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bioRxiv - Neuroscience 2022Quote: ... Cell debris was cleared by centrifugation at 1000×g for 15 min at 4°C and protein concentration was determined by bicinchoninic acid (BCA) assay (ThermoFisher Scientific #23225). Protein lysates were resolved on Bolt™ 4-12% Bis-Tris Plus gels (ThermoFisher Scientific #NW04125BOX ...
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bioRxiv - Biochemistry 2022Quote: ... which were supplemented with additional 800 μL of RIPA buffer and incubated overnight at 4°C with 15 μL of antibody-coupled protein G Dynabeads (Thermo Fisher Scientific). Before adding the cleared chromatin ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were cleared by centrifugation at 4°C and 23,000×g for 30 min and protein concentration determined using Pierce(tm) BCA Protein Assay (Thermo Fisher Scientific, USA). 0.2 μg/μl α-casein were added to a protein amount of 1.2 mg ...
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bioRxiv - Neuroscience 2024Quote: ... Joachim Herz) for 2 h at 4°C followed by overnight incubation with 20 μL of protein G Ultralink resin (ThermoFisher Cat# 53128). The resins were washed with immunoprecipitation buffer and precipitated proteins were eluted with reducing SDS sample buffer ...
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bioRxiv - Biochemistry 2022Quote: ... The samples were vortexed for 1 min before centrifugation at ∼20,160 g for 15 min at 4°C and the supernatants were centrifuged again at ∼20,160 g for 15 min at 4°C before being dried at room temperature in a Speedvac system (Thermo Scientific, Waltham, MA).
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bioRxiv - Microbiology 2023Quote: ... Four milliliters of each bacterial resuspension in ice-cold PBS were pelleted by centrifugation (14000 g, 10 min, 4 °C) (Heraeus Fresco 21, Thermo Fisher Scientific). The cells were then resuspended in 4 mL of PBS containing 2.5% sodium dodecyl sulfate (SDS) ...
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bioRxiv - Immunology 2023Quote: ... and were transiently co-transfected with IgH and IgL expression vectors for seven days prior to monoclonal antibody purification from the culture supernatant using Protein G Sepharose 4 Fast Flow beads (Cytiva,17-0618-05) and IgG elution buffer (Thermo Fisher, 21009). After neutralization and buffer exchange to PBS ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatants were incubated with primary antibodies at 4 ºC overnight and then were incubated with protein G-agarose beads (Thermo Fisher Scientific) at 4ºC for 1 h ...
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bioRxiv - Biochemistry 2023Quote: ... The lysates were then centrifuged for 15 min at 16,000 × g at 4°C and their protein concentrations were determined using a BCA Protein Assay Kit (#23227, Thermo Fisher Scientific, USA), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... 50μg of protein per sample was separated on 4-20% or 10-20% gradient Novex Tris-glycine polyacrylamide gels (Thermo Fisher Scientific) transferred onto polyvinylidene fluoride membranes (Bio-Rad Laboratories) ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell lysates were cleared by centrifugation at 21130 g for 10 min at 4°C and quantified for protein concentration using an albumin standard (Thermo Fisher 23209) and Bradford reagent (Bio-Rad 5000006) ...