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Citations for Thermo Fisher :
8001 - 8050 of 10000+ citations for 6H 1 3 Dioxolo 4 5 g 1 benzopyran 6 one 7 8 dihydro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... The lysates were then centrifuged for 15 min at 16,000 × g at 4°C and their protein concentrations were determined using a BCA Protein Assay Kit (#23227, Thermo Fisher Scientific, USA), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Lysates were centrifuged at 12.000 x g for 20 minutes at 4 °C and the supernatants were collected for RNA digestion using RNaseI (Thermo Scientific AM2294) for 40 minutes at 25 °C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Trypsinization was stopped with FBS containing medium and cells were pelleted for 5 min at 4 °C and 300°×g and resuspended in fresh culture medium for determination of cell concentration with Countess II (Thermo Fisher Scientific). For further cultivation 100000 HEK293FTs ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were then centrifuged at 14000 g for 10 min and loaded onto a NuPAGE Novex 4-12% Bis-Tris gel (Novex Life Technologies, WC1020) and run at 120 V for 90 minutes ...
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bioRxiv - Biophysics 2023Quote: ... 0.5% NP-40 alternative) with protease inhibitors and cleared for 2 hours at 4 °C with magnetic Protein G Dynabeads (Thermo Fisher, #10009D) before overnight immunoprecipitation with 1 ug per 250ug of proteins of either normal serum IgGs or specific antibodies as listed above ...
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bioRxiv - Neuroscience 2023Quote: ... Cell lysates were obtained via centrifugation at 20,000 ×g for 10 min at 4°C and were incubated with Purified GST-RhoA (G17A)-glutathione magnetic beads (Thermo Fisher Scientific) for 1 h at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Insoluble cell components were separated at 20000 × g for 15 min at 4°C and total protein concentration was assessed using BCA assay (Thermo Fisher Scientific). Protein denaturation was performed in SDS sample buffer at 95°C for 10 min.
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bioRxiv - Cell Biology 2024Quote: ... Cell lysates were centrifuged at 15,000 x g at 4°C for 20 min and protein concentration was quantified using bicinchoninic acid (BCA) assay kit (Thermo Fisher Scientific). 10-25 µg of proteins were resolved in 8% SDS-PAGE gels before being transferred onto polyvinylidene (PVDF ...
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bioRxiv - Neuroscience 2024Quote: ... Striatal homogenates were immunoprecipitated by end-over mixing for 30 min at 4°C with Dynal protein G magnetic beads (Invitrogen, Carlsbad, CA) pre-coated with 50ug each of two anti-GFP antibodies (clones 19C8 and 19F7 ...
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bioRxiv - Neuroscience 2024Quote: ... 500 μg protein was incubated with rabbit IgG control or rabbit anti-tau antibody overnight at 4°C and pulled down by protein A/G Magnetic Agarose beads (Thermo Scientific, 88802) for 6 h at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... Lysates were then cleared by centrifugation at 3000 g for 15 minutes at 4°C and the supernatants were loaded on a Zeba de-salt spin column (Thermo Fisher Scientific). Proteins were quantified using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Blots were incubated with primary antibody for either 1 hour at room temperature or at 4°C overnight using the following dilutions: 1:1,000 mouse-α-HA 2.2.14 (Thermo Fisher 26183), 1:4,000 rabbit-α- PfClpP and 1:4,000 rabbit-α-PfClpR47 (kind gifts from Walid Houry) ...
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bioRxiv - Cell Biology 2020Quote: ... before incubating with 4 μg/ml of secondary antibodies for 1 h at room temperature (Alexa fluro 488, 594, 647, Invitrogen). Samples were imaged on an Olympus FV3000 confocal laser scanning microscope.
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bioRxiv - Developmental Biology 2021Quote: ... and incubated overnight at 4°C with an antibody against either Perlecan (1:50, Life Technologies, MA1-06821, Carlsbad, CA), Collagen2a1 (1:50 ...
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bioRxiv - Developmental Biology 2021Quote: ... and further cultured in the DMEM/F12-based medium (D-MEM/F12 supplemented with 4% KSR additive, 1% ITS-X (Gibco), 0.3 % BSA (Invitrogen) ...
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bioRxiv - Developmental Biology 2021Quote: ... double-stranded RNA was adjusted to 4 μg/μL and mixed with Rhodamine Dextran B dye (20:1) (Thermo Fisher) for visualization of injected volume ...
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bioRxiv - Developmental Biology 2020Quote: ... embryos were incubated overnight at 4°C with goat anti-rabbit Alexa Fluor 488 secondary antibody (used 1:800 dilution, A27034 Invitrogen). Finally ...
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Bidirectional neuronal migration coordinates retinal morphogenesis by preventing spatial competitionbioRxiv - Developmental Biology 2021Quote: ... zebrafish embryos were incubated at 4°C for 1 h in E3 supplemented with 500 μm of EdU (ClickiT-Alexa 488 fluorophore kit, Invitrogen) while human retinal organoids were incubated at 37°C for 1h in organoid medium supplemented with 10 μm of EdU ...
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bioRxiv - Biophysics 2022Quote: ... was dialyzed overnight in 1 L of 1 × TB at 4 °C in a Slida-A-lyzer mini dialysis unit with 3,000 MWCO (Thermo Fisher) to reduce the amount of glycerol in the sample ...
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bioRxiv - Cell Biology 2022Quote: ... cells were kept overnight at 4°C in IMDM medium supplemented with 10% FBS (HyClone) and 1% penicillin-streptomycin (Thermofisher). Staining was performed for 20min at room temperature (RT ...
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bioRxiv - Molecular Biology 2020Quote: 6xHis-tagged REV-ERBβ LBD (4 nM) was incubated with anti-His antibody conjugated to terbium (1 nM) (ThermoFisher #PV5863) and FITC-labeled NCoR ID1 or NCoR ID2 peptide (400 nM ...
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bioRxiv - Molecular Biology 2021Quote: ... and 10 μL of boiled beads (representing 4 %) in 1 x NuPAGE loading dye were loaded onto NuPAGE Bis-Tris gels (MW<100 kDa) (Invitrogen) or Tris-Glycine gels (Bio-Rad ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were fixed in 4% paraformaldehyde-PBS for 20 minutes at 37°C then stained in 1:2000 diluted Hoechst 33232 (ThermoFisher) for 10 mins.
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bioRxiv - Cell Biology 2022Quote: ... Cells are differentiated for 4 days in embryonic body (EB) differentiation medium (DMEM/F12 [Hyclone], 20% FBS, NEAA [1×, GIBCO] ...
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bioRxiv - Cell Biology 2022Quote: ... BJ-5ta cells (CRL-400, ATCC) were cultured in a 4:1 mixture of DMEM and Medium 199 (Thermo Fisher) containing 10% FBS ...
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bioRxiv - Molecular Biology 2021Quote: ... overnight at 4 °C and further incubated with secondary antibodies (goat anti-rabbit Alexa fluoro 488, Invitrogen, 1:200 dilution). The nuclei were stained with DAPI (1:1000) ...
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bioRxiv - Biophysics 2019Quote: ... overnight at 4°C and after PBS washing were incubated with DAPI (1:1000, Thermo Fisher Scientific, Waltham, MA, USA) and Alexa Fluor 532 goat anti mouse (1:200 ...
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bioRxiv - Cell Biology 2019Quote: ... incubated with anti mTOR antibodies 1:200 overnight at 4°C and processed as above using anti-rabbit secondary antibody Alexa 555 (Invitrogen) at 1:400 in 3% BSA ...
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bioRxiv - Cell Biology 2019Quote: The mRFP-CLASP2γ U2OS cells expressing H2B-GFP were cultured in glass coverslips using DMEM without phenol red and supplemented with 25 mM of 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES; Gibco) and 10% FBS ...
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bioRxiv - Neuroscience 2019Quote: ... in PBS containing 0.3% Triton X-100 (PBS-Tx) overnight at 4◦C followed by incubation with goat-anti-rat AlexaFluor647 (1:500; ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2019Quote: ... staining was then performed in permeabilization buffer for 1 hour at 4°C before washing and resuspending cells in DPBS (Gibco). All flow cytometry data was acquired using Attune FxT flow cytometer and data was analysed using FlowJoTM software V10.
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bioRxiv - Developmental Biology 2020Quote: ... were filleted using standard protocols and fixed in 4%PFA for 15min and stained with a rabbit anti-GFP antibody (1:500, Invitrogen). Secondary antibodies conjugates with Alexa488 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... sections were incubated for 24 h at 4°C with Alexa Fluor488 chicken anti-goat IgG (1/1000, Molecular Probes) or Alexa Fluor488 goat anti-rabbit IgG (1/1000 ...
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bioRxiv - Developmental Biology 2021Quote: ... Slides were incubated with primary antibody overnight at 4°C followed by a 10min DAPI stain (1:30,000 at room temperature, Invitrogen, D1306) and 1h incubation with secondary antibodies including ...
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bioRxiv - Genetics 2019Quote: ... cDNA was synthesized with ~200 ng of purified RNA using 1/4 reactions of SuperScript IV VILO Master Mix with EZ DNase (Invitrogen). Another 10% of the cell culture was used for DNA extraction using 15 uL of QuickExtract (Lucigen) ...
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bioRxiv - Immunology 2021Quote: ... then the lysate equivalent to 2×105 cells was mixed at a 1:4 ratio with 4x LDS buffer containing 10% sample reducing agent (Invitrogen). Samples were heated at 95°C for 5 minutes and collected by centrifugation before loading.
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bioRxiv - Cell Biology 2021Quote: ... The obtained protein samples were separated on 4-12% Bis-TRIS NuPAGE gel in 1 × MES buffer (Thermo Fisher Scientific) and transfer to nitrocellulose membrane before blocking with 5% non-fat milk dissolved in 1 × TRIS-buffered saline (TBS ...
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bioRxiv - Bioengineering 2021Quote: ... the cells were incubated with 2 μM EthD-1 and 4 μM Calcein AM solutions of cell viability/cytotoxicity kit (Invitrogen) for 20 minutes ...
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bioRxiv - Bioengineering 2021Quote: ... at 4°C for overnight and Alexa conjugated secondary antibodies (1:1000, Goat anti-rabbit IgG Alexa fluor 647, Invitrogen, A21245 ...
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bioRxiv - Neuroscience 2021Quote: A stable dispersion of SWCNTs was achieved based on previously published protocols28,43 through tip-sonication for 1 h at 30 % power at 4 °C (Fisher Scientific Model 120 Sonic Dismembrator ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated with goat anti-rabbit IgG Alexa 488 secondary antibody (1:500 for 2 h at 4°C; Invitrogen) in solution with PBS ...
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bioRxiv - Cancer Biology 2021Quote: 20 µg of proteins extracted in SDS buffer (1% SDS supplemented with protease and phosphatase inhibitors) were loaded on to a 4-12% gradient SDS-PAGE gel (Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... PBMC1-2 and MS-5 cells were cultured alone and in co-culture in a 4:1 AML/PBMC-stromal ratio and 500,000 cells/mL density in α-MEM (Gibco) with 12.5% (v/v ...
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bioRxiv - Cell Biology 2021Quote: ... overnight at 4°C and then with 1:250 goat anti-mouse Alexa Fluor 488 (A11008; Life Technologies, Waltham, MA) and 1:250 goat anti-rabbit Alexa Fluor 647 (A21244 ...
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bioRxiv - Plant Biology 2021Quote: ... a mouse anti-FLAG primary monoclonal antibody was used (anti-DYKDDDDK FG4R, 1 μg/mL overnight at 4 °C, Invitrogen), and horseradish peroxidase (HRP)-coupled goat anti-mouse IgG was used as a secondary antibody horseradish peroxidase (0.5 μg/mL for 1 hr at 21 °C ...
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bioRxiv - Microbiology 2020Quote: Purified cysts were stained following brain tissue homogenization using a 1:4 dilution of 12.3mg/ml of Hoechst stain (Thermo Scientific) and incubated for 20-30 minutes prior to being viewed on a Zeiss Axiovert microscope ...
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bioRxiv - Cell Biology 2020Quote: ... Wells were washed with PBS and stored overnight at 4°C in EL08.1D2 cell culture media supplemented with 1% P/S (Life Technologies).
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bioRxiv - Microbiology 2021Quote: ... bacteria from 1 ml samples were harvested by centrifugation and resuspended in 500 μL of FM™ 4-64FX (Invitrogen) at 5 μg/mL ...
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bioRxiv - Immunology 2020Quote: ... Twenty-four h post transfection MEFs and HEK293Ts were treated with 4 μg/ml and 1 μg/ml puromycin (Invitrogen) for 48 h ...
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bioRxiv - Neuroscience 2021Quote: ... 20-25 µg of RIPA or SDS lysate or 1 µg of S4 lysate were separated in a 4-20% Tris-glycine gel (Novex, Invitrogen) and transferred to PVDF membranes ...