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Citations for Thermo Fisher :
8101 - 8150 of 10000+ citations for 6H 1 3 Dioxolo 4 5 g 1 benzopyran 6 one 7 8 dihydro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... The plasmids were mixed with 100 μl of PEI (4:1 v/w PEI:DNA ratio) in 500 μl of pre-warmed OptiMEM II reduced serum medium (Gibco, 1985070), after 10-15 min incubation at RT ...
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bioRxiv - Neuroscience 2022Quote: ... the culture was fixated by removing the remaining medium and adding 1 mL of a 4% paraformaldehyde (PFA, Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... for 1hr before being incubated overnight at 4°C in and primary antibodies (1:1000 mouse anti-V5 Tag monoclonal Antibody (Invitrogen) and anti-Actin monoclonal antibody (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were washed 3 times for 20 minutes each time in 1x PBS/0.3% Triton X-100/10% NDS at room temperature and incubated overnight at 4°C with secondary antibodies (Thermo Fisher Scientific Cat# A-21206, RRID:AB_2535792, 1:500; Thermo Fisher Scientific Cat# A-21203 ...
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bioRxiv - Plant Biology 2023Quote: ... 4 μl of the DNAseI reaction was mixed with 1 μl of oligo(dT)18 0.5 μg/μl (Thermo Scientific), and incubated at 65°C for 5 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... overnight at 4°C followed by incubation with donkey anti-goat Alexa Fluor 594 secondary antibodies (1:200, A-11058; Invitrogen) and rabbit anti GFP (1:100 ...
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bioRxiv - Neuroscience 2022Quote: ... they were incubated for 24 hours at 4 °C with Alexa Fluor 594-conjugated donkey anti-goat IgG (1:300, A-11058, Invitrogen), Cy3-conjugated goat anti-rabbit IgG (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... the tissues were washed five times with PBS and then permeabilized for 1 hour with 0.2% TritonX-100 supplemented with 4% goat serum (Thermo Fisher). The permeabilization solution was also used to generate a primary antibody solution ...
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bioRxiv - Neuroscience 2022Quote: ... before being incubated 72hours at 4°C with the following primary antibodies: chicken anti-GFP (1:1000, Life Technologies, #A10262) and rabbit anti-FMRP (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... WAKO) overnight at 4 °C followed by detection with Alexa 488 conjugated anti-rabbit secondary antibody (1:1000, Life Technologies), and Alexa 594 conjugated anti-rat secondary antibody (1:1000 ...
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bioRxiv - Developmental Biology 2022Quote: ... Overnight incubations (4°C) of the sections were carried out with mouse monoclonal anti- proliferating cell nuclear antigen (PCNA) (1:200, Invitrogen). Control sections were incubated with a mouse IgG Isotype Control (1:200 ...
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bioRxiv - Developmental Biology 2022Quote: ... 48 hpf embryos were incubated at 4°C for 1 h in E3 supplemented with 500 μm of EdU (ClickiT-Alexa 488 fluorophore kit, Invitrogen) in 10% DMSO ...
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bioRxiv - Neuroscience 2024Quote: ... denatured (70°C for 10min) protein samples (1 µg/µl) were loaded onto a NuPAGE 4-12% Bis-Tris Gel (Invitrogen) along with Chameleon 800 pre-stained protein ladder (LI-COR) ...
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bioRxiv - Molecular Biology 2024Quote: ... Glycoproteins were resolved by SDS–PAGE (approx. 1×107 cell equivalents/lane) on NuPAGE bis-Tris 4–12% gradient acrylamide gels (Invitrogen) and transferred either to nitrocellulose or PVDF membranes (Invitrogen) ...
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bioRxiv - Pathology 2024Quote: ... at 4 °C overnight followed by an Alexa Fluor 594-conjugated secondary antibody (1:500) (A-11012, Thermo Fisher Scientific). Immunofluorescence staining images were acquired using the Pannoramic Scanner software (v3.0.3.139795 RTM) ...
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bioRxiv - Cancer Biology 2023Quote: ... and then incubated overnight at 4°C in a humidified chamber with anti-γ-H2AX (1:500, Invitrogen MA5-38225), anti-perilipin-2 (1:200 ...
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bioRxiv - Physiology 2024Quote: ... sections were incubated overnight at 4°C in primary antibody solution containing 10% goat serum and antibodies against laminin β1 (1:100; MA5-14657, Invitrogen) or laminin β1γ1 (1:200 ...
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bioRxiv - Biochemistry 2024Quote: ... The sample was dialysed in 1 L of purification buffer at 4°C using Snakeskin Dialysis Tubing (3.5 k MWCO, Thermo Fisher), followed by an overnight dialysis under the same conditions.
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bioRxiv - Microbiology 2024Quote: ... Proteins were purified at 4°C by the batch method over 1 mL Ni-NTA resin according to the manufacturer’s instructions (HisPur, Thermo Scientific). All buffers were 50 mM NaPO4 ...
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bioRxiv - Neuroscience 2023Quote: ... followed by an overnight incubation at 4 °C with 20 μg ml-1 laminin (Thermo Fisher Scientific, 23-017-015). After being thoroughly washed with PBS ...
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bioRxiv - Immunology 2024Quote: ... the membranes were probed overnight at 4°C with anti-mouse CASP-1 (Cat# 14-9832-82; Thermo-Fisher Scientific) or anti-mouse mature IL-1β (Cat# 63124S ...
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bioRxiv - Neuroscience 2023Quote: ... followed by a goat anti-rabbit Alexa Fluor 546 secondary antibody (1:1000; overnight at 4 °C; # A-11035, Invitrogen). Sections were mounted in a medium containing 50 % glycerol ...
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bioRxiv - Cell Biology 2024Quote: ... at 4°C o/n and then with 40 μL of pre-blocked (1 mg/ml BSA) Dynabeads protein A (ThermoFisher). Beads were sequentially washed with Wash buffer 1 (20 mM Tris HCl pH 8.1 ...
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bioRxiv - Developmental Biology 2024Quote: ... the samples were washed extensively in PBS and then incubated overnight at 4°C in blocking solution with the appropriate concentration of secondary antibodies diluted at 1:500 and with DAPI (Invitrogen) diluted at 1:1000 ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were centrifuged at 500 ×g for 5 minutes at 4°C and washed and resuspended with ice-cold FACS Buffer (2 or 10% FBS in 1× HBSS (Gibco) or 1X PBS) ...
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bioRxiv - Cancer Biology 2024Quote: ... and incubated overnight at 4°C in a humified chamber with anti-AKAP12 antibody (Thermo Fisher #PA5-21759, 1:200) or anti-Ki67 (CST #9449 ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant was incubated rotating for 1 h at 4 ºC on 1.25 mL bed of HisPur Ni-NTA agarose beads (ThermoFisher Scientific), pre-equilibrated in lysis buffer ...
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bioRxiv - Cell Biology 2024Quote: ... Myoblasts were cultured in growth medium consisting of 1 vol 199 Medium/4 vol DMEM (Life Technologies, Carlsbad, CA, USA) supplemented with 20% fetal calf serum (Life technologies ...
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bioRxiv - Developmental Biology 2023Quote: ... Membranes were blocked for 2hrs at RT in 5% milk powder and then incubated overnight (o/n) at 4°C with primary rabbit anti-GFP antibody (1:1,000; Molecular Probes). Membranes were washed 3x for 10min in 1X TBST ...
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bioRxiv - Cell Biology 2022Quote: ... pMDG2.1-VSV-G and p8.91-GAG-POL in a ratio of 4:1:2 to a total of 10 μg of DNA using Lipofectamine 2000 (ThermoFisher) as per the manufacturer’s instructions overnight ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated for 4 hours with Streptavidin AlexaFluor-488 (S32354) and anti-mouse CY5 (A10524) (ThermoFisher Scientific; 1:1,000) where appropriate in a 5% NGS and 0.25% Triton-X in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... in PBT then incubated for 24–48 hr at 4 °C in the primary antibody mouse anti-GFP (1:500 in blocking buffer, Ref# A11120, Invitrogen). The sample was washed in PBT for 3 hr at 25 °C then incubated in the secondary antibody AF488-conjugated goat-anti-mouse (1:500 in blocking buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... in blocking buffer overnight in 4°C and stained with secondary AlexaFluor633 donkey anti-goat antibodies (Invitrogen, #A21082; 1:200) for 1 hour at RT ...
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bioRxiv - Molecular Biology 2023Quote: ... and D1-D4 (domains 1-4) with C-terminal 6x-His or StrepII tags were transiently transfected using the Expi293 expression system (ThermoFisher)7 ...
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bioRxiv - Developmental Biology 2023Quote: ... A total of 10 washes with PBSFT were performed before secondary antibodies (diluted 1 in 500) (see Table 4) and Hoechst (Hoechst 33342 trihydrochloride trihydrate, Invitrogen Mol Probes H3570 ...
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bioRxiv - Biophysics 2023Quote: Cryo-EM data collection for the hCLDN-4/cCpE/COP-1 complex was performed on a Titan Krios G3i (ThermoFisher) equipped with a Gatan K3 direct electron detector and BioContinuum GIF at PNCC ...
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bioRxiv - Microbiology 2023Quote: ... for 16 h at 4°C and with a secondary fluorescent antibody (Alexa-488 anti-sheep diluted 1:50,000; Thermo Fisher) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were washed again in 0.5% Triton PBS and incubated for 2 h at room temperature or overnight at 4 °C in secondary antibody (donkey anti-goat 647, 1:1000, A-21447, ThermoFisher) in carrier solution ...
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bioRxiv - Molecular Biology 2023Quote: ... hESCs were seeded at 2×104 cells/cm2 in Matrigel coated flasks and cultured for 4 days using mTeSR-1 and passaged with TrypLE (ThermoFisher). Subsequently ...
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bioRxiv - Plant Biology 2023Quote: ... Blotted membranes were blocked with PBS containing 4% w/v skimmed milk powder followed by incubation with 1:1000 dilution of mouse anti-GFP antibody (GF28R, Invitrogen) in blocking buffer followed by incubation with 1:5000 dilution of goat anti-mouse IgG secondary antibody ...
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bioRxiv - Biochemistry 2022Quote: ... Tetramers of streptavidin conjugated to biotinylated 3FTX were formed via 60 min incubation of 4:1 3FTX:streptavidin-APC (Thermo Fisher) at room temperature prior to use.
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bioRxiv - Biochemistry 2022Quote: ... The filtrate was then incubated for 1 h rotating at 4 °C with 2 mL Ni-NTA resin (Thermo Fisher) per 1.5 L of expression culture ...
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bioRxiv - Immunology 2023Quote: Retrovirus was generated by transfecting BHK cells (ATCC) or Phoenix cells (laboratory stocks) with 1-4 μg plasmid using the Lipofectamine 2000 Transfection Reagent (Invitrogen). Supernatants were collected at 24 and 48 hours post-transfection and added to MEFs pre-cultured in six-well plates ...
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bioRxiv - Developmental Biology 2023Quote: ... Brains were then washed using 0.5% PBT (4×15 minutes) and incubated 1:500 with secondary antibody (anti-mouse/rabbit Alexa-488/647, Invitrogen/ThermoFisher) for 4 hours at RT before transferring to 4°C for three overnights ...
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bioRxiv - Neuroscience 2023Quote: ... rinsed 3X in 0.1% PBST and incubated in goat anti-mouse Alexa Flour 647 antibody overnight at 4°C (1:300, ThermoFisher A32728). Images were acquired using a Zeiss 880 Laser Scanning Confocal inverted microscope with a Plan-Apochromat 63x/1.4 oil DIC M27 lens ...
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bioRxiv - Biochemistry 2023Quote: ... The proteins were resolved by SDS-PAGE (approximately 1 × 107 cell equivalents/lane) on NuPAGE bis-Tris 4–12% gradient acrylamide gels (Invitrogen) and transferred to nitrocellulose membrane (Invitrogen) ...
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bioRxiv - Biochemistry 2022Quote: ... diluted in PBS-T overnight at 4°C and detected with 1:2000 HRP-conjugated goat anti-chicken secondary antibody (#A16054, Invitrogen) diluted in PBS-T during 1 hour at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were incubated overnight at 4°C with the following primary antibodies: TMEM163 (Thermofisher, cat # PA5-114329; 1:100), CD83 (Biolegend ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were washed with assay buffer (PBS containing 0.2% BSA and 1 mM CaCl2) and resuspended in assay buffer with 2 μM Fluo-4-AM (Life Technologies). Fluo-4-AM was prepared as a 1 mM stock in DMSO ...
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bioRxiv - Synthetic Biology 2023Quote: ... the membrane was incubated overnight at 4°C with primary antibody solution (1:1000 dilution for anti-TSG101 [Abcam, ab30871], anti-Calnexin [ThermoFisher, PA5-19169] and anti-Syntenin-1 [Origene ...