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Citations for Thermo Fisher :
7851 - 7900 of 10000+ citations for 6H 1 3 Dioxolo 4 5 g 1 benzopyran 6 one 7 8 dihydro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... The virus was spun at 500 g for 5 min and the supernatant filtered through a 45 μm polyethersulfone filter (Fisher Scientific) and subsequently concentrated ...
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bioRxiv - Microbiology 2021Quote: ... Cell nuclei were stained with DAPI (5 μg/mL) for 30 minutes and slides were mounted with Fluoromount-G™ mounting medium (Thermofisher) overnight in a dark chamber ...
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bioRxiv - Cell Biology 2021Quote: ... magnetic beads were bound with ~10 μg rabbit polyclonal anti-Tulp1 antibodies (mTulp1N) and beads were cross-linked with 5 mM BS3 (Bis[sulfosuccinimidyl] suberate, Thermo Scientific) in 20 mM HEPES for 30 min ...
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bioRxiv - Cell Biology 2021Quote: ... 800 μL of differentiated HL-60 cells were spun down at 200 × g for 5 minutes and resuspended in 100 μL of 5x concentrated CellMask Deep Red (Invitrogen, C10046) in L-15 ...
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bioRxiv - Biochemistry 2022Quote: The remainder of the cytoplasmic fraction was added to 5 µL of washed Pierce Protein G agarose beads (Thermo Fisher Scientific), and the mixture was rotated 1-2 hours at 4°C to pre-clear ...
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bioRxiv - Biochemistry 2022Quote: MT-4 cells with and without lentiviral plasmids were collected by centrifugation (225× g, 5 min, at 20 °C) and proteins were extracted using lysis buffer (RIPA Thermo Scientific, cOmplete™ ...
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bioRxiv - Cancer Biology 2024Quote: ... Microstructures were then collected into a 50-ml conical tube by centrifugation (179 × g for 5 min) and washed with HBSS without calcium and magnesium (Gibco, #14175095) containing 0.1% BSA (Ambion ...
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bioRxiv - Cell Biology 2023Quote: ... The filtered cell mixtures were spun at 1,500 x g for 5 minutes and resuspended in myoblast growth medium (GM) (F-10 medium (Thermo Fisher Scientific) containing 20% FBS ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Amoebae were grown from frozen spores at room temperature on 100 x 15mm plates filled with 30mL SM/5 agar (2 g glucose (Fisher Scientific), 2 g Bacto Peptone (Oxoid) ...
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bioRxiv - Genetics 2022Quote: ... Cells were passaged twice per week following a 5-min incubation in PBS containing 0.2 g/L EDTA (Invitrogen, Carlsbad, CA).
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bioRxiv - Bioengineering 2023Quote: ... Cells were pelleted by centrifugation at 1,000 g for 5 min and lysed with M-PER™ mammalian protein extraction reagent (Thermo Scientific) supplemented with Halt™ protease and phosphatase inhibitor cocktail (Thermo Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... the cells were washed with DPBS and were pelleted by centrifugation at 1,000 g for 5 min and lysed with M-PER™ mammalian protein extraction reagent (Thermo Scientific) supplemented with the Halt™ protease and phosphatase inhibitor cocktail (Thermo Scientific ...
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bioRxiv - Genetics 2023Quote: ... 4 μg of total RNA from testes was reverse-transcribed using 5’ RACE System for Rapid Amplification of cDNA Ends (Invitrogen, 18374058) and synthesized cDNA was PCR-amplified ...
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bioRxiv - Immunology 2021Quote: ... macrophages were preincubated for 5 min at 37 °C with 2.5 µM of Yo-Pro-1 iodide (Life Technologies) after galvanic current application or 1% triton X100 (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2021Quote: ... The fixed cells were washed with PBS and blocked with 5% goat serum for 1 hr (Thermo Fisher, 31872). Mouse anti-T7 primary antibody (Millipore Sigma ...
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bioRxiv - Evolutionary Biology 2021Quote: ... to the tube was added a solution of 0.269 mg of P58 mixed 5:1 by volume with salmon sperm DNA (Invitrogen), followed by 3 mL of 39.52% polyethylene glycol ...
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bioRxiv - Bioengineering 2022Quote: ... Membranes were probed overnight at 4 °C with primary antibodies diluted to 1/2000 in 5% BSA and 0.1% sodium azide in PBS using specific total MET (#37-0100 Invitrogen), total ERK2 (#SC-154 Tebu-bio) ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were counterstained by 5 min incubation in 300 µM DAPI intermediate solution (1:1,000, Molecular Probes, Cat# B34650). Section were then washed with PBS three times ...
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bioRxiv - Genetics 2020Quote: ... 1μl (equivalent to a 5:1 insert:vector ratio) was ligated into a blunt-ended TOPO vector (Zero Blunt TOPO PCR Cloning Kit, ThermoFisher) and transformed into E.coli ...
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bioRxiv - Neuroscience 2019Quote: ... Thiol reactive fluorescent probe IAEDANs (1,5-IAEDANS, 5-((((2-iodoacetyl)amino)ethyl)amino)naphthalene-1-sulfonic Acid) was purchased from ThermoFisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... followed by permeabilization in 5% Triton-X for overnight and incubation in phalloidin Alexa-546 conjugate (Invitrogen, 1:500). All staining experiments were repeated three times with larvae from independent clutches.
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bioRxiv - Biochemistry 2020Quote: ... 0.5% w/v sodium dodecyl sulfate) to which 400 μl of warm 5:1 Acid Phenol:Chloroform (Ambion, cat. # AM9722) were added and the resulting phase-separated mixture was agitated by vortexing at maximum speed for 15 sec ...
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bioRxiv - Biochemistry 2020Quote: COS-1 and HEK293T cells were cultured at 37 °C and 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM, Gibco) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2021Quote: ... and subsequently for 1 h in chicken polyclonal antibody against NF200 (RRID: AB_2313552, Aves) diluted 1:1000 in 5% normal goat serum (Cat #PCN5000, ThermoFisher), 0.5% TritonTM X-100 (Cat #9002-93-1 ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was diluted to 50 µM and labelled with a 5:1 molar excess of fluorophore:protein with either AlexaFluor 488 C5- Maleimide (Invitrogen) (AF488) ...
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bioRxiv - Systems Biology 2021Quote: ... A 1:5 dilution of this protein solution was quantified using a Pierce BCA protein assay kit (Thermo scientific). For analysis of the relative composition of amino acids ...
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bioRxiv - Microbiology 2020Quote: ... African green monkey kidney epithelial Vero E6 cells were grown in Dulbecco’s modified Eagle’s medium (DMEM) with 5% fetal bovine serum (FBS; HyClone Laboratories, South Logan, UT) and 1% antibiotic/ streptomycin (Gibco). Human lung adenocarcinoma epithelial Calu-3 2B4 cells were maintained in a high-glucose DMEM supplemented with 10% FBS and 1% penicillin/streptomycin at 37°C with 5% CO2 ...
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bioRxiv - Cancer Biology 2021Quote: For this analysis 1/5 of the total EV pellet per sample was resuspended in 10 μL PBS (Gibco) and stored at 4°C for up to 7 days ...
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bioRxiv - Cancer Biology 2022Quote: ... and resuspended in flow buffer plus 5 μL of propidium iodide (PI) staining solution (10 μg mL-1, Invitrogen) per sample ...
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bioRxiv - Cell Biology 2022Quote: ... After incubation cells were incubated for 5 min at RT with TMR-phalloidin (0.83 μg/mL) and Hoechst 33342 (1 μg/mL, Invitrogen) in PBS and washed with blocking buffer before imaging on a Zeiss Axiovert 200 equipped with an Andor Luca camera.
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bioRxiv - Microbiology 2022Quote: ... RNAs from both treated and untreated arms of the experiment were separated on a 5% denaturing acrylamide:bisacrylamide (19:1) gel alongside a RiboRuler Low Range RNA Ladder (ThermoFisher) and the gel fixed ...
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bioRxiv - Immunology 2022Quote: ... CD4+ and CD8+ T-cells were stained with LysoTracker Red DND-99 (Invitrogen; 5 μM; 1 h; 37°C), fixed (3% PFA ...
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bioRxiv - Developmental Biology 2020Quote: ... The testes were then washed with two portions of PBST and incubated in PBSTX (0.1% Tween-20 and 0.3% Triton X-100 in 1×PBS) with 5% normal goat serum (Life Technologies) at room temperature for at least 1 h ...
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bioRxiv - Biophysics 2021Quote: ... cell nuclei were stained by exposure for 5 min to 1 mg/100 ml Hoechst 33342 (Thermo Fisher Scientific) in water.
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bioRxiv - Bioengineering 2021Quote: ... The slides were counterstained with DAPI (1:5,000) for 5 min and mounted with Prolong Diamond Antifade Mountant (Invitrogen). A Nikon A1 laser-scanning confocal microscope was used to acquire confocal images ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were passaged two times a week 1:5 in culture-treated flasks using TrypLE dissociation media (ThermoFisher 12604039).
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bioRxiv - Developmental Biology 2019Quote: ... 1:200 dilution for 5 minutes prior to plating and BA2 tissue was soaked in DiI (V22889, Thermofisher Scientific), 1:30 dilution for 30 minutes ...
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bioRxiv - Biophysics 2019Quote: ... After rinsing with a solution containing NPE-caged ATP (adenosine 5′-triphosphate, P3-(1-(2-nitrophenyl) ethyl) ester) (Invitrogen) (0 to 1 µM ...
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bioRxiv - Physiology 2020Quote: ... Membranes were blocked with 5% BSA for 1 hour and western blotted with anti-Myc antibody (Invitrogen; MA1-21316) overnight at 4 °C ...
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bioRxiv - Bioengineering 2019Quote: ... at 300g for 5 minutes and resuspended in 250μL SP-TALP supplemented with 100μg*mL−1 heparin-fluorescein conjugate H7482 (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... RNAs were ethanol-precipitated and ligated to 5pmol 5’adapters (Table 1) with 10U T4 RNA ligase (Ambion AM2140) supplemented with 12.5% PEG8000 and 2U µl-1 RNasin Plus for 16hr at 16°C before being purified with Oligo Clean & Concentrator ...
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bioRxiv - Microbiology 2020Quote: ... The nuclei were stained with DAPI (1:10,000) diluted in PBS for 5 min and mounted with an antifade reagent (ThermoFisher). Images were acquired with a Leica TCS SP5 confocal microscope system.
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bioRxiv - Genomics 2021Quote: ... The cells were finally stained with calcofluor white (5 μg/mL for 1 h at 37°C; Invitrogen, USA). Alternatively ...
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bioRxiv - Systems Biology 2021Quote: ... 1 μL of samples and 5 μL of standards were diluted into 95 μL of 1X SYBR Green (Invitrogen) in TE buffer and mixed by pipette ...
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bioRxiv - Neuroscience 2020Quote: ... The tissue was washed twice with 5 ml Hibernate-A including 1 mg/ml protease inhibitor (Thermo Fisher, 78442) and briefly triturated in 2 ml of Hibernate-A medium using fire-polished glass Pasteur pipettes until a single-cell suspension was obtained ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by 1 h incubation with 1:1,000 anti-mouse Alexa Fluor 488 conjugated secondary antibody (diluted with PBS containing 5% BSA, Invitrogen) at RT in the dark ...
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bioRxiv - Microbiology 2020Quote: ... all grown at 37 °C and 5% CO2 and supplemented with 1% penicillin-streptomycin (Life Technologies Australia Pty. Ltd) A549 cells were grown in Ham’s F-12K (Kaighn’s ...
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bioRxiv - Cell Biology 2020Quote: ... washed two times and suspended with Williams E medium supplemented with 5 % CCS and 1 mM glutamine (Invitrogen, CA). To separate live from dead cells ...
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bioRxiv - Bioengineering 2019Quote: ... was biotinylated using a 5:1 molar excess of Ez-Link™ Sulfo-NHS-LC-Biotin (Thermo-Fisher Scientific) overnight at 4°C ...
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bioRxiv - Genetics 2020Quote: ... 5-10 μg of backbone was digested using 1 μL Fastdigest Eco31I (aka BsaI, Thermo Fisher Scientific, cat. FD0293) or Fastdigest BpiI (aka Thermo Fisher Scientific ...