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Citations for Thermo Fisher :
7801 - 7850 of 9391 citations for Mumps Virus Nucleoprotein Strain L Zagreb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... penicillin-streptomycin (15140-122-100ml) L-Glutamine (25030-081-100ml) and 1x PBS (14190-144-500ml) were purchased from Gibco. FBS (F0926-500ml) ...
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bioRxiv - Cell Biology 2021Quote: ... Donkey anti-Mouse/Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody conjugated to Alexa 488 (Life Technologies, 1/2000) were used to detect the primary antibody staining.
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bioRxiv - Cell Biology 2022Quote: ... and 300 μl of resuspended cells in growth medium were transferred to a well of an 8-well chambered coverglass slide (Nunc). Cells were then imaged 48 hours after addition of growth medium using a Zeiss LSM 880 confocal microscope.
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bioRxiv - Biochemistry 2022Quote: ... Blots were washed 3x with PBS and probed with Alexa Fluor 680 Goat Anti Mouse and Goat anti-Rabbit IgG (H+L) Alexafluor Plus 800 secondary antibodies (1/2000, Invitrogen) in 1% casein/PBS and visualized using a LiCor Odyssey infrared imager.
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bioRxiv - Biochemistry 2022Quote: ... was supplemented with 1 mM L-lysine and 0.5 mM L-arginine of the individual SILAC labels (Silantes 211104113, 201204102, 211604102, 201604102) as well as 1.7 mM light L-proline and 1 x GlutaMAX (Gibco 35050061). For full labelling the intermediate or heavy SILAC label was introduced during six passages in intermediate or heavy DMEM for SILAC ...
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bioRxiv - Cancer Biology 2022Quote: Cells were starved of L-methionine for 30 minutes and subsequently incubated with 50 μM homopropargylglycine (HPG; Life Technologies #C10186) for 1 to 4 hours in treatment media ...
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bioRxiv - Neuroscience 2022Quote: ... and donkey anti-mouse IgG (H&L) highly cross-adsorbed secondary antibody Alexa Fluor 568 (Thermo Fisher Scientific, A10037, 2110843) were used at 1:1,000 dilution ...
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bioRxiv - Immunology 2022Quote: ... Tissue sections were treated with L-lysine (0.1%, SigmaAldrich) to reduce background and non-specific interactions before being blocked using 3% BSA (Invitrogen, ThermoFisher) for 30 minutes at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: HL-60 promyelocytic cell line (DSMZ, Germany) was grown in RPMI1640 medium with L-glutamine (Biowest) and 10% Fetal Bovine Serum (Gibco) at 37°C in a 5% CO2 ...
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bioRxiv - Cell Biology 2022Quote: ... RPE1 cells were cultered in advanced DMEM/F-12 + non-essential amino acids + 110mg/L Sodium Puruvate (Gibco, Lot#12634010) supplemented with 10% (vol/vol ...
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bioRxiv - Developmental Biology 2022Quote: ... Alexa Fluor 647-conjugated goat anti-rabbit IgG (H+L) antibody (Abcam, #ab150079, or Thermo Fisher Scientific, #A21247, 1:1000), Blebbistatin (Merck Millipore ...
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bioRxiv - Biochemistry 2022Quote: ... Two µl of the sample was resolved on a PepMap RSLC C18 column (75 µm i.d. x 50 cm; Thermo Scientific) at the flow-rate of 300 nl/min using 0.1% formic acid/acetonitrile gradient system (2-22% acetonitrile in 150 min ...
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bioRxiv - Biochemistry 2022Quote: ... and HEK-293T cells (ATCC) were cultured in Dulbecco’s high-glucose minimal essential medium (DMEM, 4.5 g/L glucose, Gibco, Waltham, MA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... the culture medium was replaced with RPMI□+ □B27+insulin medium (RPMI 1640, Thermo Fisher; 2 mM L-glutamine, Thermo Fisher; 1×□B27 supplement with insulin ...
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bioRxiv - Cell Biology 2022Quote: ... Gels were then washed in PBST three times for 10min while gently shaking and subsequently incubated with secondary antibodies Alexa Fluor 488 goat-anti-rabbit IgG (H+L) (Invitrogen), Alexa Fluor 594 goat-anti-mouse IgG (H+L ...
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bioRxiv - Cell Biology 2022Quote: ... cells plated on glass bottom cell culture dish were loaded with 10 μmol/L Fura-2 acetoxymethyl ester (Molecular Probes) and 0.1% pluronic F-127 (Sigma ...
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bioRxiv - Immunology 2022Quote: ... were generated by plastic adherence for 1h in complete RPMI 1640 (680 μM L-Glutamine and 10 mM Hepes, GIBCO) supplemented with 5% pooled human serum (The Blood Bank ...
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bioRxiv - Immunology 2022Quote: ... 0.1 mM EDTA) for 5 minutes at room temperature and then neutralized with B cell media (RPMI 1640 with L-glutamine (Gibco) supplemented with 15% fetal bovine serum (Corning) ...
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bioRxiv - Physiology 2022Quote: ... The pellet was resuspended in DMEM (4.5 g/L glucose, 10% fetal bovine serum and penicillin/streptavidin; all ThermoFisher Scientific) and the cells were seeded on non-coated tissue culture plates ...
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bioRxiv - Immunology 2022Quote: ... ELISA against S-2P was performed in the absence or presence of sodium thiocyanate (NaSCN) and developed with HRP-conjugated goat anti-monkey IgG (H+L) secondary antibody (Invitrogen) and SureBlue 3,3′,5,5′-tetramethylbenzidine (TMB ...
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bioRxiv - Pathology 2022Quote: ... and re-suspended (500µL per kidney) in low salt buffer (20mM HEPES-KOH, Sigma Aldrich #H0527, 0,15M KCl, Thermofisher #AM9640G, 10mM MgCl2, Thermofisher #AM9530G ...
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bioRxiv - Genomics 2022Quote: ... and dialyzed against 1 L of PB in a 3 ml Slide-A-Lyzer™ Dialysis Cassettes (Thermo Fisher Scientific) at RT for 3 hrs ...
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bioRxiv - Microbiology 2022Quote: ... after which the supernatant was clarified via filtration and subsequently sterile filtered (0.2 μm) into a 20 L bioprocess bag (Thermo Fisher) prior to protein A purification ...
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bioRxiv - Microbiology 2022Quote: ... Parasites were stained by transferring 10 µL of the 1:10 dilutions to a 96-well plate containing 100 µL of 1x SYBR Green (S7563, Invitrogen) in PBS ...
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bioRxiv - Immunology 2022Quote: ... and HFs were placed individually into a 96-well plate slot with 100 µl supplemented medium (William’s E plus 1% penicillin-streptomycin antibiotics, 1% L-glutamine (Invitrogen-Gibco), 0.01% hydrocortisone (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... and rabbit anti-mouse IgG (H+L) cross-adsorbed secondary antibody (Alexa Fluor® 488 (ThermoFisher Scientific, cat# A-11059)) were used at a 1:500 dilution as secondary antibodies ...
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bioRxiv - Bioengineering 2022Quote: ... CK+DCIR medium consists of epithelial base medium (50% DMEM [Gibco], 50% F-12 Nutrient Mixture [Gibco], 15 mM HEPES [Corning], 4 mM L-glutamine [Gibco)] 1% P/S [Gibco] ...
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bioRxiv - Immunology 2022Quote: ... RPMI-1640 medium supplemented with 10% heat inactivated FCS and final concentrations of 100 U/mL penicillin and 100 μg/mL steptomycin and 2mM L-Glutamine (Gibco). Cells were incubated with OVA peptide (323-339 ...
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bioRxiv - Cell Biology 2022Quote: ... each siRNA was transfected at a concentration of 220 pmol/l with transfection reagent in Opti-MEM Medium (Life Technologies). Cells were incubated with the siRNA complexes for 4 h ...
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bioRxiv - Cancer Biology 2022Quote: ... These three cell lines were grown at 5% CO2 and 37° C in RPMI 1640 without L-Glutamine media supplemented with heat inactivated 10% FBS (Gibco), penicillin/streptomycin (Gibco) ...
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bioRxiv - Neuroscience 2020Quote: ... cells stably expressing the human Nav1.1 channel (gift of Dr. Alfred L. George, Jr.) were grown in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific) containing 10% FBS (Sigma) ...
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bioRxiv - Microbiology 2020Quote: ... MAVS (L-024237-00-0005) or non-targeting control (D-001810-10-05) (Dharmacon) using Lipofectamine RNAiMAX Transfection Reagent (Invitrogen). Transfection medium was replaced after 24h with DMEM medium supplemented with 10 FBS ...
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bioRxiv - Microbiology 2021Quote: ... 2 μL of 10 mM NBD-(linezolid-7-nitrobenz-2-oxa-1,3-diazol-4-yl)-amino-D-alanine (NADA) (Thermo Fisher) was added to each tube and incubated at 37° C ...
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bioRxiv - Neuroscience 2019Quote: Frozen vials of human skin fibroblasts were thawed and grown in T75 flasks for seven days in DMEM media supplemented with 20% foetal calf serum and L-glutamine (all from Gibco). Cells were trypsinised and plated onto 13 mm untreated glass coverslips (50,000 per coverslip) ...
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bioRxiv - Cancer Biology 2020Quote: ... Libraries were then PCR amplified and indexed using Phusion Hot Start II High Fidelity Polymerase (Thermo Scientific, F 549-L). An equal molar pool of each library was sequenced.
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bioRxiv - Developmental Biology 2021Quote: ... and goat anti-rabbit IgG (H+L) conjugated with Alexa Fluor 488 was from Molecular Probes (A11008, 1/400 dilution). Positive cells were counted from blinded images using the Cell Counter tool of Fiji.
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bioRxiv - Neuroscience 2021Quote: ... 50 μL Miltenyi beads per sample were pre-blocked with 48 μL 1× wash buffer and 2 μL yeast tRNA (Invitrogen), rotating for 20 min at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... in TBST buffer with 0.5% BSA and goat α-mouse IgG (H+L) HRP conjugated secondary antibody (1:10,000, Invitrogen A28177) were used to detect His-tagged proteins on the blot ...
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bioRxiv - Cell Biology 2021Quote: ... and 1 μg/ml Na-p-tosyl-l-lysine chloromethyl ketone (TLCK) for 20 min at 37°C and precipitated with CD31-coated magnet beads (Dynabeads; ThermoFisher). These vessels were further cultured in Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Neuroscience 2021Quote: ... The cells were plated on poly-L-lysine hydrobromide-coated six-well culture plates for 8 days in Neurobasal Medium (GIBCO) supplemented with B27 ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were then washed 3x in HBSS and dissociated by pipetting up and down 15-20 times through a P1000 pipette in growth media (Neurobasal media supplemented with 5 % FBS, 500 nM L-glutamine, B27 (Gibco) and Pen-strep ...
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bioRxiv - Biophysics 2021Quote: ... The bullae were removed from mouse temporal bones and submerged in cold Leibovitz media (L-15, 21083-027, Life Technologies). The organ of Corti sensory epithelium was microdissected from the cochleae ...
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bioRxiv - Neuroscience 2020Quote: ... coverslips were washed three times in PB 0,12 M and incubated with a goat anti-rabbit IgG (H+L) highly cross-adsorbed AlexaFluor A594 (1:1.000, Life Technologies) antibody diluted in the blocking solution for 2H at RT ...
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bioRxiv - Neuroscience 2020Quote: ... slices were washed three times in PB 0,12 M and incubated with a goat anti-rabbit IgG (H+L) highly cross-adsorbed AlexaFluor A594 (1:1.000, Life Technologies) diluted in blocking solution for 2H ...
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bioRxiv - Neuroscience 2020Quote: ... The PFC was dissected out from the sections and the tissue stored in 1 mL ice-cold Leibovitz’s L-15 medium containing 1 μL SUPERase RNase inhibitor (20 U/μl, ThermoFisher Scientific). Specifically ...
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bioRxiv - Bioengineering 2020Quote: The relatively long-term cell viability in the liver models was assessed after 72 hours of culture using Live/Dead Viability Kit (L-3224, ThermoFisher). To ensure diffusion of staining dyes into the center of the centimeter-size liver model and also to better visualize the cells in the center region ...
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A chlorzoxazone-baclofen combination improves cerebellar impairment in spinocerebellar ataxia type 1bioRxiv - Neuroscience 2020Quote: ... Secondary antibodies used were as follows: Alexa Fluor 488 goat anti-mouse IgG (H+L) (1:200; Invitrogen, Cat. #A11001), Alexa Fluor 488 goat anti-rabbit IgG (H+L ...
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bioRxiv - Molecular Biology 2022Quote: ... The first strand cDNA for the expressed H and L chain was synthesized using a commercially available Superscript III reverse transcription kit (Invitrogen). The 25 μL reverse transcription reaction mixture consisted of 1X PCR buffer ...
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bioRxiv - Neuroscience 2022Quote: ... Mixed glial cells were plated onto poly-L-lysine-coated flasks and grown in DMEM supplemented with 10% fetal bovine serum (FBS) (Gibco). After a 24 h incubation ...
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bioRxiv - Neuroscience 2022Quote: ... For each coverslip 15,000 cells were allowed to settle in a 40 μl drop for about 30 min and then each well was filled with 500 μl growth medium: NeurobasalA/B27 (Invitrogen) supplemented with GlutaMax (0.25% ...