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Citations for Thermo Fisher :
7701 - 7750 of 9391 citations for Mumps Virus Nucleoprotein Strain L Zagreb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... Fixed cells were dried onto the poly-L-Lysine coated cover slip and mounted on a slide with SlowFade Diamond (Invitrogen). 3D SIM visualisation was performed using a v4 DeltaVision OMX 3D-SIM system (Applied Precision ...
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bioRxiv - Synthetic Biology 2019Quote: ... 300 μl of cell culture was added to individual wells in a 96-Well Optical-Bottom Plate with Polymer Base (ThermoFisher) for fluorescence measurement using FLUOstar Omega microplate reader ...
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bioRxiv - Cancer Biology 2020Quote: ... cell line GRANTA-519 waskept in culture in RPMI 1640 medium supplemented with 10% heat-inactivated FBS 2 mM L-glutamine and 1% penicillin/streptomycin antibiotics (Gibco® ...
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bioRxiv - Molecular Biology 2019Quote: ... Coverslips were washed 3 times for 5 min each with PBS and incubated with 1:10,000 dilution Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody Alexa Fluor 568 secondary antibody (#A-11036, Invitrogen) for 1 hour at room temperature in the dark ...
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bioRxiv - Bioengineering 2019Quote: ... Secondary antibodies included goat anti-rabbit IgG (H+L) alexa fluor 594 conjugate (1:200, Thermo Fisher Scientific, Waltham, MA). An Olympus Fluoview 1000 inverted confocal microscope was used to image samples with a 20x PlanFluor dry objective (0.5NA) ...
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Differential turnover of Nup188 controls its levels at centrosomes and role in centriole duplicationbioRxiv - Cell Biology 2019Quote: ... HeLa-M and HeLa T-Rex-Flp-In were cultured in Dulbecco’s Modified Eagle Medium (DMEM) with 4.5 g/L glucose (Thermo Fisher Scientific) and U2OS cells (ATCC ...
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bioRxiv - Microbiology 2019Quote: ... which were generated by combining defined ratios of RPMI1640 with or without L-glutamine (11875, 11.1 mM glucose, 2 mM glutamine; and, 31870, 11.1 mM glucose, no glutamine; Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2019Quote: ... in a petri dish and the striatum was dissected out and stored in 1 ml ice-cold Leibovitz’s L-15 medium containing 1μl SUPERase RNase inhibitor (20 U/μl, Thermo Fisher Scientific). The neuron nuclei were isolated using a nuclear isolation protocol25 ...
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bioRxiv - Biochemistry 2019Quote: ... 2 μg of each antibody was diluted in 30 μl PBS-T and then incubated with 5 μl protein A-coated magnetic beads (30 mg/ml, Dynabeads, 10002D, Invitrogen) for 30 min ...
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bioRxiv - Neuroscience 2019Quote: ... or 1 µg of pCMV-SPORT6.FBXL3 and 40 nM ON-TARGETplus human CUL1 (8454) siRNA SMARTpool (Dharmacon, L-004086) using the Neon transfection system (Invitrogen) following the manufacturer instructions and 1400 V ...
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bioRxiv - Biochemistry 2020Quote: ... Secondary antibodies: HRP-conjugated polyclonal goat anti-Ms and anti-Rb Cross-Adsorbed IgG (H+L) (ref. A16072 and A16104, Invitrogen), used at 1:10000 dilution in TBS-T.
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bioRxiv - Bioengineering 2019Quote: ... Cells were dissociated by 0.25% trypsin for 15 min at 37°C and plated on poly-dl-ornithine-coated cover glasses (150,000 cells/cm2) in Neurobasal medium containing B27 supplement and 0.5 mM l-glutamine (Gibco/Life Technologies). Before plating ...
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bioRxiv - Developmental Biology 2019Quote: Stage 22-24 chick embryos were removed from the egg and washed in Leibovitz’s L-15 medium (Thermo Fisher Scientific) supplemented with 1% L-Glutamine-Penicillin-Streptomycin solution (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2019Quote: ... hearts from Col1a1-3.6GFP mice were digested and Col GFP-cells were grown for 3 days in high (4.5g/L) glucose DMEM (Thermo Fisher), supplemented with 10% v/v fetal bovine serum (FBS) ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... while Gout Anti-Mouse IgG(H+L) and Alexa Fluor® 488 (1:100; Thermo Fisher Scientific K.K., Tokyo, Japan) were applied as secondary antibodies for 60 min at 20 °C ...
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bioRxiv - Immunology 2019Quote: ... Lungs were immediately sliced into 300μm thick sections on a vibratome and stained with directly conjugated Ab in complete medium (phenol-red free DMEM substituted with 10% FBS, 2mM L-glutamine and 1% Penicillin/Streptomycin, all Gibco) for 30 minutes at 37°C ...
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bioRxiv - Pathology 2020Quote: ... A 98 μl of RT-QuIC reaction mix containing tau isoform was loaded into each well of a 96-well plate (Nunc). The 10% brain homogenates were diluted at 1:200 in a dilution buffer containing 10 mM HEPES and 1.7% 1 × N2 supplement (Gibco ...
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bioRxiv - Cell Biology 2019Quote: IPHHs were thawed in a water bath set to 37°C and suspended in 10 ml Leibovitz’s L-15 Medium (Life Technologies) supplemented with Glutamax (Life Technologies ...
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bioRxiv - Physiology 2019Quote: ... MEFs were transfected with 30 nM control siRNA or a smartpool on-target plus siRNA for mouse CHOP (Dharmacon - L-062068- 00-0005) using Lipofectamine RNAi MAX (Invitrogen) according to the manufacturer’s instruction ...
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bioRxiv - Biophysics 2021Quote: U2OS 2-6-3 cells containing the stably integrated lacO/tetO reporter gene cluster (Janicki et al., 2004) were grown in DMEM (1 g/l glucose, Gibco) without phenol-red supplemented with 10 % tetracycline-free fetal calf serum (FCS) ...
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bioRxiv - Genetics 2021Quote: ... Samples (2μl) were then mixed with 10μl of Gene Scan 400HD ROX dye standard (1:50) in HiDi-formamide (Life Technologies) followed by denaturation at 95°C for 5 min and a hold at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... The cleared supernatants were incubated with 50 µl of beads pre-coated with 40 µg of anti-PTBP1 antibodies (clone BB7) or mouse IgG Isotype Control (Invitrogen). Immunoprecipitations were conducted overnight at 4°C with constant shaking ...
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bioRxiv - Cell Biology 2020Quote: ... derived from WHO strain MNYC/BZ/62/M379 (Beneke et al., 2017) were grown in M199 medium with Earle’s salts and L-glutamine (Life technologies) supplemented with 26 mM NaHCO3 ...
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bioRxiv - Immunology 2021Quote: ... The CBMC layer was isolated and subsequently washed twice with pre-warmed base medium (BM) (RPMI-1640 + L-Glutamine, Gibco), and then complete medium (CM ...
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bioRxiv - Immunology 2021Quote: ... HEK293T cells cultured in DMEM-10 medium (DMEM supplemented with 10% FBS, 100U penicillin, 100µg streptomycin, and 2mM L-glutamine. DMEM was purchased from Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... Virus-infected cells were detected using in-house raised rabbit anti-S polyclonal antibody along with peroxidase-conjugated goat anti-rabbit IgG (H+L) secondary antibody (Invitrogen). MN titers represented the reciprocal of the highest serum dilution that yielded >50% reduction in OD values as compared to wells containing virus only ...
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bioRxiv - Neuroscience 2020Quote: Acute coronal sections from P0 Plxna4+/+ or Plxna4-/-;Htr3a-GFP brains were prepared as described above and SVZ/IZ stream microdissected in ice-cold L-15 medium (Gibco) under a fluorescence scope ...
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bioRxiv - Neuroscience 2021Quote: ... 25 µl of supernatant was mixed with loading buffer and resolved by SDS-PAGE in 4- 12% Bis–Tris gels (Invitrogen) under reducing conditions and electro-blotted onto nitrocellulose membranes ...
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bioRxiv - Microbiology 2021Quote: ... The plate was washed 3 times before adding 50 μl of 1:1,000 diluted Goat anti-Mouse IgG H+L-HRP antibody (Invitrogen, # 31430) to the plate and incubated for 1 h at RT ...
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bioRxiv - Cell Biology 2021Quote: ... Beads were washed three times with 1 mL cold buffer L before being boiled in 20 μL of 1x LDS sample buffer (Invitrogen) supplemented with 50 mM DTT ...
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bioRxiv - Neuroscience 2021Quote: ... trypsinized for neurons dissociation and plated in poly-L-lysine treated coverslips and maintained in medium containing B27-supplement (GIBCO), glutamax (GIBCO ...
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bioRxiv - Cancer Biology 2021Quote: ... for the last 30 min of the drug treatment in L-methionine-depleted medium containing 5% dialyzed fetal calf serum (FCS; Gibco). Harvested cells were washed with PBS ...
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bioRxiv - Bioengineering 2022Quote: ... using vacuum filtration (Berg and Turner, 1990) and resuspended into 5% RMB (RMB, including 9.13 g/L Na2HPO4 (Fisher Scientific), 4.87 g/L NaH2PO4·H2O (Amresco) ...
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bioRxiv - Genetics 2021Quote: ... 60 μl of 10X restriction enzyme buffer provided together with the corresponding restriction enzyme (viewpoint 1: 10X Buffer B (ER0211, Invitrogen); viewpoint 2 ...
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bioRxiv - Bioengineering 2021Quote: Samples were prepared for Raman microscopy by drop casting 10 μL of DNA-SWCNT solution (10 mg/L) onto cleaned glass slides (Coverslip 24×55×0.15 mm, Fisher Scientific). Raman spectra were recorded at an excitation wavelength of 532 nm at 100%relative power with 5 s exposure time ...
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bioRxiv - Bioengineering 2021Quote: SKOV-3 ovarian cancer cells (supplied by the Laboratory for Cell and Protein Regulation at the University of Otago) were cultured in Earle’s salts and L-Glutamine positive MEM medium (GIBCO®) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2021Quote: ... MDA-MB-231 cells and MSCs were cultured in high-glucose DMEM + L-glutamine (Gibco, Thermo Fisher Scientific, Waltham, MA) supplemented with 10% fetal bovine serum (Genesee Scientific ...
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bioRxiv - Bioengineering 2021Quote: ... MDA-MB-231 cells and MSCs were cultured in high-glucose DMEM + L-glutamine (Gibco, Thermo Fisher Scientific, Waltham, MA) supplemented with 10% fetal bovine serum (Genesee Scientific ...
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bioRxiv - Bioengineering 2020Quote: ... RNA was isolated from ciPTEC grown in tubular scaffolds or in L-Dopa coated well plates (control) using a PureLink RNA mini kit (Invitrogen), and cDNA was prepared using 500 ng sample RNA templates using iScript cDNA Synthesis Kit (BioRad) ...
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bioRxiv - Bioengineering 2020Quote: ... Cultures were labeled with either goat anti-rabbit IgG (H + L) Alexa Fluor 555 antibody (1:100) (Thermo Fisher Scientific) or rabbit anti-mouse FITC antibody (1:100 ...
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bioRxiv - Neuroscience 2020Quote: ... Caspase-3 (1:5000 anti-Rbt; CST; cat # 9662S) ERO1-L (1:1000 anti-Rbt; Thermo Fisher Scientific; cat # 702709) and β-Actin (1:3000 anti-ms ...
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bioRxiv - Neuroscience 2021Quote: ... using 1ng/μL of cDNA according to the manufacturer’s protocol with a QuantStudio 12 K Flex Real-Time PCR System (Applied Biosystems). The following primers specific for mouse were used:
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bioRxiv - Neuroscience 2021Quote: ... and were cultured on poly-D-lysine and poly-L-ornithine (100 μg/mL)-coated plates in Neurobasal-A medium containing antioxidant-free B27 supplement (Gibco) and 2 mM L-glutamine ...
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bioRxiv - Neuroscience 2021Quote: Mouse lumbar spinal cord were extracted from five C57.Bl6 mice and maintained in neurobasal media (supplemented with B27 and L-Glutamine, Invitrogen). Lumbar spinal cord tissue was cut to 400μm thickness and incubated on high density microelectrode arrays (3Brain ...
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bioRxiv - Neuroscience 2021Quote: ... protein lysate samples were also extracted from invitro lumbar spinal cord slices prepared from six C57bl6 male adult mice and slices maintained in Neurobasal media (supplemented with L-Glutamine, Invitrogen)) and exposed to vehicle or 1mM DMOG for 24 hours ...
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bioRxiv - Neuroscience 2020Quote: ... The transfected neurons were plated on glass-bottomed 35 mm plastic dishes and incubated at 37°C for 24-36 hours in L-15 medium with 10% fetal bovine serum (FBS; Gibco) and 1x penstrep (Gibco) ...
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bioRxiv - Biophysics 2020Quote: ... epsin 1,2,3-KD HeLa cells were plated after electroporation on poly-L-lysine-coated plastic petri dishes (Sarstadt Ø18 mm) in the DMEM media (Gibco) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Biophysics 2020Quote: ... and plated on the poly-L-lysine coated glass coverslips (Marienfeld, Cat No. 0117580; Ø18 mm) in the DMEM media (Gibco) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2021Quote: ... The viral spike protein was stained with an in-house developed rabbit anti-spike antibody and goat anti-rabbit IgG (H+L) highly cross-adsorbed antibody with Alexa Fluor 488 (Invitrogen). Images were acquired using FluoView FV10i Confocal Laser Scanning Microscope (Olympus) ...
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bioRxiv - Cancer Biology 2020Quote: ... rinsed and incubated with secondary antibody Alexa Fluor 594 Goat Anti-mouse immunoglobulin G (IgG) (H/L) antibody (1:1000, Invitrogen) for 60 minutes at room temperature ...