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Citations for Thermo Fisher :
7901 - 7950 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... or Kif9 using Lipofectamine RNAiMax (Invitrogen) according to manufacturer instructions and incubated for 36-48 hours prior to experiments.
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bioRxiv - Cell Biology 2024Quote: ... and a Volta phase plate (ThermoFisher Scientific). Grid maps at 233 nm/pixel and lamella maps at 2.2 nm/pixel were first collected ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were transfected with 50 nM control (negative control siRNA#1 from Ambion) or Kif9 using Lipofectamine RNAiMax (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... 5’-UAGUGACUUCUGGAAAUUCag-3’ (antisense) (Ambion by Life Technologies); siRNA#4 ...
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bioRxiv - Cell Biology 2024Quote: Cryo-FIB milling was carried out on a dual beam Aquilos1 equipped with a cryo-transfer system and stage (ThermoFisher Scientific). Prior to milling ...
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bioRxiv - Cell Biology 2024Quote: ... 5’-UAGUGACUUCUGGAAAUUCag-3’ (antisense) (Ambion by Life Technologies); siRNA#4 ...
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bioRxiv - Cell Biology 2024Quote: ... 8X RNAse cocktail (RNAseA and RNAseT1, both targeting single-stranded RNA; Thermo Fisher) diluted in PBS was added to one slide (treated) ...
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bioRxiv - Cell Biology 2024Quote: ... The cell suspension was loaded into a cytofunnel and spun for 5 min at 1200 rpm onto a poly-lysine coated slide using a cytocentrifuge (Shandon Cytospin 4, Thermo Fisher Scientific). The slides were immediately transferred to a coplin jar containing 100 ml of fixative (45% acetic acid and 2% formaldehyde in DEPC water ...
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bioRxiv - Cell Biology 2024Quote: ... Nuclei were pelleted at 800g for 10 min at 4°C and washed twice in buffer 1 and once in freezing buffer (1M Tris pH 8, 100% glycerol, 100mM MgAc2, 0.5M EDTA, 1M DTT, 1x PTase inhibitor cocktail Roche, 20U/µl SUPERase In Ambion). Nuclei were resuspended in freezing buffer ...
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bioRxiv - Cell Biology 2024Quote: ... 1M MgCl2, 100% Triton X-100, 100mM EGTA, 1M DTT, 1x PTase inhibitor cocktail Roche, 20U/µl SUPERase In Ambion, 1M CaCl2), dounced in a 1m dounce homogenizer with a loose pestle 25 times ...
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bioRxiv - Cell Biology 2024Quote: ... Astrocyte cultures were plated in Dulbecco’s modified Eagle’s medium (DMEM) (Thermo Fisher Scientific), supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2024Quote: ... the secondary antibodies were purchased from Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... and 10% FBS (Gibco) at 37°C in 5% CO2 into 75 cm2 tissue culture flasks ...
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bioRxiv - Cancer Biology 2024Quote: ... Blots were washed again in 1X TBST (x3) and then incubated with SuperSignalTM West Pico PLUS Chemiluminescent substrate (ThermoFisher Scientific) in the dark for 5 min prior to imaging on an InvitrogenTM iBrightTM 1500 imaging system ...
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bioRxiv - Cell Biology 2024Quote: ... RILPL1 cDNA was amplified with PCR and cloned into the pCR8/GW/TOPO vector (Thermo Fisher). PCR8-RILPL1 was then subcloned into pDEST-3xflag-FKBP using Gateway technology (Thermo Fisher) ...
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bioRxiv - Cancer Biology 2024Quote: ... Spheroid cultures were maintained in DMEM/F-12 medium supplemented with 1% glutaMAX (Gibco, cat. 35050061), 100 U/mL penicillin-streptomycin (cat ...
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bioRxiv - Cancer Biology 2024Quote: ... using a QuantStudio 3 real-time PCR instrument (applied biosystems, Thermo Fisher Scientific). The following primers were used ...
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bioRxiv - Cancer Biology 2024Quote: ... at a concentration of 200 pmols using Lipofectamine 2000 reagent (cat# 11668027, Invitrogen).
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bioRxiv - Cancer Biology 2024Quote: ... Anti-mouse CD31-biotin (cat# 13-0311-82, Thermo Fisher, Waltham, MA, USA) was administered by tail vein i.v ...
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bioRxiv - Cancer Biology 2024Quote: ... 555 or 647 conjugated secondary antibodies targeting the primary antibody species (Invitrogen, Eugene, OR, USA), and nuclei were stained with 4 ...
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bioRxiv - Cancer Biology 2024Quote: ... with the ViiA7 Real Time PCR system (Applied Biosystems). Primers used for qRT-PCR are listed in Table S6.
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were separated by SDS-PAGE (NuPAGE NOVEX Bis-Tris 4–12% gels (Life Technologies) and transferred to PVDF membranes ...
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bioRxiv - Cancer Biology 2024Quote: ... HEK293T cells were plated at 70% confluency the night before transfection and cultured in DMEM (Gibco, 11995065) with 10% FBS (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... Selection with 3 μg of puromycin (ThermoFisher Scientific, A1113803) was conducted 36 hours post-transduction ...
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bioRxiv - Cancer Biology 2024Quote: ... SDS-Page was performed in MES-SDS running buffer (20x, ThermoFisher Scientific, NP0002) at 140 V for ∼1 hour or until the dye front had run off the gel ...
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bioRxiv - Cancer Biology 2024Quote: ... and washed in PBS and briefly in ddH2O then mounted on glass slides using ProLong GoldTM anti-fade glass mounting medium (P36980, Fisher Scientific, UK). Z-stack images were acquired using a Leica SP8 confocal microscope at magnifications indicated in legends using 2048×2048 pixel resolution ...
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bioRxiv - Cancer Biology 2024Quote: ... Pelleted neurons were resuspended in neuronal growth media (DMEM-F12 with HEPES (Fisher Scientific 11-330-032), penicillin-streptomycin (ThermoFischer Scientific 15140-122) ...
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bioRxiv - Cancer Biology 2024Quote: ... were diluted in Opti-MEM (ThermoFisher Scientific, 31985070) and added to 67NR cells at ∼60% confluency ...
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bioRxiv - Cancer Biology 2024Quote: ... The base medium for all organoid cultures (and co-cultures) included DMEM (Gibco, 11995065), L-glutamine (ThermoFisher ...
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bioRxiv - Cancer Biology 2024Quote: ... EO771 cells were maintained in RPMI with 10% FBS and 10 mM HEPES (ThermoFisher Scientific 15-630-080). 67NR ...
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bioRxiv - Cancer Biology 2024Quote: ... RNase T1 (ThermoFisher Scientific, EN0541) was used at concentration of 2000 U/ ml ...
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bioRxiv - Cancer Biology 2024Quote: ... washed with PBS and incubated 5 minutes with PBS containing 2 µg/mL Hoechst (33342, Fisher Scientific, UK) and washed in PBS and briefly in ddH2O then mounted on glass slides using ProLong GoldTM anti-fade glass mounting medium (P36980 ...
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bioRxiv - Cancer Biology 2024Quote: ... Py8119 cells were cultured in Ham’s F-12K (Kaighn’s) medium (ThermoFisher Scientific, 21127022) with 10% FBS ...
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bioRxiv - Cancer Biology 2024Quote: ... A BCA assay kit (Thermo Scientific, PI23225) was used to quantify the amount of protein in each sample ...
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bioRxiv - Cancer Biology 2024Quote: ... Equal amounts of protein were loaded in 4-12% Bis-tris Mini Protein Gels (Fisher Scientific, NP0336PK2). SDS-Page was performed in MES-SDS running buffer (20x ...
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bioRxiv - Cancer Biology 2024Quote: ... whole blood (100µL) was diluted in 400 µL of complete medium (RPMI 1640 (Gibco) and incubated 3h with GolgiPlug (BD Biosciences) ...
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bioRxiv - Cancer Biology 2024Quote: ... After red cell lysis (1-step Fix/Lyse solution, Invitrogen), cells were permeabilized with the permeabilization buffer (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... N2 and B27 supplements (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... The gels were transferred at 100 V for 1.5 hours at 4°C in transfer buffer (ThermoFisher Scientific, NP0006) onto PVDF membranes (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... The siRNA and LipofectamineTM RNAiMAX transfection reagent (ThermoFisher Scientific, 13778150) were diluted in Opti-MEM (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: Cell proliferation was assessed using the PrestoBlue Cell Viability Reagent (ThermoFisher Scientific, Stockholm, Sweden) and the MTS reagent (G3582 ...
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bioRxiv - Cancer Biology 2024Quote: ... SNU-499 and Hep3B cells (10,000 cells/well) were seeded in multi-well 96 plates (Corning-Falcon, ThermoFisher Scientific, Stockholm, Sweden). After three days ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were incubated with 2.5 µM DCFH-DA (2’,7’-dichloro-dihydro-fluorescein-diacetate; excitation at 495 nm, emission at 520 nm; Life Technologies, Europe BV, Stockholm, Sweden) in Hank’s buffered saline solution without phenol red for 30 min at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were then treated with RBC Lysis Buffer (Invitrogen #00-4333-57) for 5 min at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... and surface proteins were biotinylated with 0.5 mg/ml Premium Grade Sulfo-NHS-LC-Biotin (Fisher Scientific) in PBS at 4°C for 30 min ...
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bioRxiv - Cancer Biology 2024Quote: The dissociated single cells from the GEMM animal models were cultured in ultra-low attachment 96-well plates (ThermoFisher Scientific, 174927) in EpiCult™-B (mouse ...
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bioRxiv - Cancer Biology 2024Quote: ... and 1% TritonX-100) containing Halt Protease and Phosphatase Inhibitor Single-use Cocktail (Thermo Fisher Scientific) and incubated 15LJmin on ice ...
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bioRxiv - Cancer Biology 2024Quote: ... and the GeneArt Gibson Assembly HiFi Master Mix (Life Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... SNU-499 or Hep3B cells seeded in multi-well 6 plates (Corning-Falcon, ThermoFisher Scientific, Stockholm, Sweden) in their approporiate culture medium (control or glutamine-deprived) ...
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bioRxiv - Cancer Biology 2024Quote: ... and rabbit anti-GFP (polyclonal anti-GFP rabbit IgG, A11122 Invitrogen, 1:000, or polyclonal anti-GFP rabbit IgG, A-6455 ThermoFisher, 1:2000) antibodies were then used ...