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Citations for Thermo Fisher :
7851 - 7900 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... The concentration of the extracted total RNA was determined using the Nanodrop 2000 system (Thermo Fisher Scientific, Waltham, MA, USA). The Mir-X miRNA First-Strand Synthesis Kit (TAKARA ...
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bioRxiv - Cancer Biology 2024Quote: ... The molarity of the libraries were quantified by using a Qubit dsDNA HS Assay Kit (ThermoFisher – Q32851) together with a Tapestation (Agilent) ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were maintained in advanced RPMI-1640 medium (Invitrogen, Frederick, USA), supplemented with 5% fetal bovine serum (FBS) ...
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bioRxiv - Cancer Biology 2024Quote: ... Both cell lines were grown in the following growth medium: RPMI 1640 + GlutaMAX™ (Gibco) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2024Quote: ... and 1% penicillin-streptomycin (Gibco) in a humidified atmosphere at 37°C and 5% CO2.
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bioRxiv - Cancer Biology 2024Quote: ... Enzymatic treatments were done in staining buffer supplemented with 3 mM magnesium chloride with 1:200 dilutions of RNase T1 (Thermo Fisher Scientific, EN0541), ShortCut RNase III (New England Biolabs ...
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bioRxiv - Cancer Biology 2024Quote: ... mouse MHC-I PE conjugated H-2Kb clone AF6-88.5.5.3 (Invitrogen #12-5958-82).
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bioRxiv - Cancer Biology 2024Quote: ... An Ultimate 3000 nano UHPLC system coupled with a Q Exactive HF mass spectrometer (Thermo Fisher Scientific) with an ESI nanospray source was used.
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bioRxiv - Cancer Biology 2024Quote: ... Tris-Acetate Protein Gel (ThermoFisher) or NuPAGE 4 to 12% ...
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bioRxiv - Cancer Biology 2024Quote: ... 5 μg of total RNA were processed using the Ribominus™ Eukaryote System v2 (ThermoFisher). The rRNA-depleted ...
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bioRxiv - Cancer Biology 2024Quote: Cells were lysed by RIPA buffers (ThermoFisher) with 1x Halt™ Protease Inhibitor Cocktail (ThermoFisher ...
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bioRxiv - Cancer Biology 2024Quote: ... PEG-4000 at concentrations ranging from 10 to 100 µM were transferred to a 96 well optical black/clear bottom plate (Thermo Scientific) and pre-incubated for 3 min at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... Ovarian cancer cell line Kuramochi (JCRB Cell Bank) was cultured in RPMI 1640 (Gibco) supplemented with 10% FBS and 1% Penicillin-Streptomycin antibiotics ...
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bioRxiv - Cancer Biology 2024Quote: ... The resulting cDNA was then used for quantitative PCR (qPCR) using SYBR™ Green PCR Master Mix (Applied Biosystems), depending on the specific gene targets ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 10% (vol/vol) fetal bovine serum (FBS, Thermo Fisher Scientific) and 1% Penicillin-Streptomycin antibiotics (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... as a radical initiator and Alexa Fluor™ 488 hydrazide (2 mg/ml) (Thermo Fisher) diluted in 10mM Tris buffer (pH = 7.48) ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were transfected with 30 nM of siRNAs using the RNAiMAX transfection reagents (Life Technologies) on two consecutive days ...
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bioRxiv - Cancer Biology 2024Quote: ... was cultured in Dulbecco’s Modified Eagle’s Medium (Gibco) supplemented with 10% (vol/vol ...
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bioRxiv - Cancer Biology 2024Quote: ... siRNAs were transfected into D2A1 cells using Lipofectamine RNAiMAX (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: Nascent RNA sequencing was conducted using the Click-iT™ Nascent RNA Capture Kit (Invitrogen). Cells were treated with or without CBPD-409 for 2 and 4 hours ...
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bioRxiv - Cancer Biology 2024Quote: ... and transferred to 0.45-μm nitrocellulose membranes (ThermoFisher). The membranes were blocked with blocking buffer (Tris-buffered saline ...
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bioRxiv - Cancer Biology 2024Quote: ... with 1x Halt™ Protease Inhibitor Cocktail (ThermoFisher) and denatured in the complete NuPage 1× LDS/reducing agent buffer (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: Transfection experiments were performed following the Lipofectamine 2000 reagent (Invitrogen) protocol provided by the manufacturer ...
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bioRxiv - Cancer Biology 2024Quote: ... and 1% Penicillin-Streptomycin antibiotics (Thermo Fisher Scientific). Ovarian cancer cell line Kuramochi (JCRB Cell Bank ...
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bioRxiv - Cancer Biology 2024Quote: ... The protein concentration was measured by Pierce BCA Protein Assay Kit (ThermoFisher). 15-30 µg protein was loaded and separated on NuPAGE 3 to 8% ...
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bioRxiv - Cancer Biology 2024Quote: ... and p53C WT and M237I with Alexa FluorTM 488 (Thermo Scientific) fluorescent dyes using the manufacturer’s specifications ...
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bioRxiv - Cancer Biology 2024Quote: ... and denatured in the complete NuPage 1× LDS/reducing agent buffer (Invitrogen) with 15-minute incubation at 70 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... The qPCR reactions were performed in QuantStudio 5 Real-Time PCR system (Applied Biosystems), and the data were analyzed using the ΔΔCt method to quantify gene expression levels ...
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bioRxiv - Cancer Biology 2024Quote: ... 1% NEAA and 1% penicillin-streptomycin (Gibco). siRNAs were transfected into D2A1 cells using Lipofectamine RNAiMAX (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: Turbidity assays were performed in a Varioskan Lux 3020-81205 Microplate Reader (Thermo Scientific). P53C ...
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bioRxiv - Cancer Biology 2024Quote: ... Telomeric DNA was identified by hybridization with a Biotin-OO-(CCCTAA)4 PNA probe (TelC, Biosynthesis) followed by incubation with Alexa Fluor 350-conjugated NeutrAvidin (Molecular Probes), followed by two rounds of incubation first with a biotinylated anti-avidin antibody (Vector ...
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bioRxiv - Cancer Biology 2024Quote: Nanoflow UPLC: Ultimate 3000 nano UHPLC system (ThermoFisher Scientific)
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bioRxiv - Cancer Biology 2024Quote: ... Secondary staining was done with anti-rabbit Alex Fluor 647 (Thermo Fisher, A32733), and the endogenous mCherry signal was boosted with anti-alpaca Alexa Fluor 568 RFP-booster (Chromotrek ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were then incubated with Alexa Fluor 488 (Invitrogen #A11034) or Alexa Fluor 594 (Invitrogen #A11012 ...
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bioRxiv - Cancer Biology 2024Quote: ... Secondary antibodies for western blots were purchased from Invitrogen (Waltham ...
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bioRxiv - Cancer Biology 2024Quote: ... and RNA was extracted using RNAqueous Micro Total RNA Isolation Kit (Invitrogen #AM1931), according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... 1mM sodium pyruvate (Gibco), 10 mM HEPES (Gibco) ...
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bioRxiv - Cancer Biology 2024Quote: ... that consisted of an µ-precolumn (C18 PepMap100, 5 µm, 100 Å, 5 mm × 300 µm; Thermo Scientific), and an analytical column (120 EC-C18 ...
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bioRxiv - Cancer Biology 2024Quote: ... and stimulated with α-CD3/α-CD28 Dynabeads (Gibco, cat. 11131D) in the presence of 10 ng/mL recombinant MIF (Biolegend ...
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bioRxiv - Cancer Biology 2024Quote: ... Sorted T cells were labelled with 2μM CellTrace Violet (Invitrogen, cat. C34557) and stimulated with α-CD3/α-CD28 Dynabeads (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... 2,688 fractions were analyzed by RPLC-MS/MS using a nanoflow LC system (EASYnano HPLC system, Thermo Scientific) coupled online with LTQ Orbitrap ELITE mass spectrometer (Thermo Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... 1% nonessential amino acids (Gibco), 1mM sodium pyruvate (Gibco) ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells are harvested by pipetting the cells loose from the flask or with enzymatic detachment using TrypLE (ThermoFisher) or Accutase (Sigma Aldrich) ...
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bioRxiv - Cancer Biology 2024Quote: ... in RPMI1640 containing 10% dialyzed FBS and 1 % penicillin/streptomycin cocktail (Gibco). SILAC labeling was to discriminate FBS derived proteins from cell proteins.
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bioRxiv - Cancer Biology 2024Quote: ... washed x 3 in TBS-T and developed with a chemiluminescent reagent (SuperSignal West Femto, Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2024Quote: siRNA for mouse-IGF1R was constructed in vitro using the Silencer siRNA Construction Kit (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... and fixable viability dye efluor 506 (Invitrogen, cat. 65-0866-14) for FACS sorting of live CD3+ T cells on a Sony SH800S machine ...
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bioRxiv - Cell Biology 2024Quote: ... siRNA#4: 5’-AUAGCGUUUCUUCUAACUGGGCAGC-3’ (Invitrogen). siRNAs #2 and #4 significantly decreased the mRNA levels to the greatest extent and both had the same mitotic phenotypes ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were cultured in RPMI-1640 (Gibco/Thermofisher and Cytiva) with a 10% final concentration of FBS (Hyclone/Cytiva ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were cultured in RPMI-1640 (Gibco/Thermofisher and Cytiva) with a 10% final concentration of FBS (Hyclone/Cytiva ...