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Citations for Thermo Fisher :
8151 - 8200 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... and 100 U/ml of penicillin/streptomycin (Invitrogen, 15140130). Chemicals ...
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bioRxiv - Cell Biology 2024Quote: ... human BAK was cloned into a version of the retroviral Peak12-MMP vector containing an IRES-hygromycin cassette for selection in 200 μg/ml hygromycin B (Invitrogen, 10687010). The cDNAs for all BH3-only members ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were incubated with a goat anti-mouse secondary antibody conjugated with Alexa Fluor 488 (1:500, Thermo Fisher Scientific) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... transfected with 40nM siRNAs (Supplemental Table S1) using RNAiMAX (Invitrogen), incubated in growth media for 24h ...
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bioRxiv - Cell Biology 2024Quote: ... followed by 3 PBS washes and mounting using ProLong Gold (Invitrogen) and 18mm square glass coverslips ...
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bioRxiv - Cell Biology 2024Quote: ... Real-time quantitative PCR was performed to analyze relative gene expression levels using SYBR Green Master mix (Applied Biosystems) following manufacturer indications ...
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bioRxiv - Cell Biology 2024Quote: ... US) culturing in DMEM medium (GibcoTM) supplemented with 10% PBS and 1% 100x antibiotic-antimycotic (Anti-Anti, Thermo Fisher Scientific, US). Cells were cultured in a 5% CO2 incubator at 37 °C and the medium was renewed twice a week ...
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bioRxiv - Cell Biology 2024Quote: ... 1 μg of total RNA was reverse transcribed with High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems). Real-time quantitative PCR was performed to analyze relative gene expression levels using SYBR Green Master mix (Applied Biosystems ...
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bioRxiv - Cell Biology 2024Quote: ... Mammospheres were grown in DMEM supplemented with B-27 (Gibco™, 17504044), human EGF (10 µg/ml ...
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bioRxiv - Cell Biology 2024Quote: ... EdU staining was performed following the instruction of Click-iT® Plus EdU Alexa Fluor® 594 Imaging Kit (C10639, Thermo Fisher Scientific). Growing cells on coverslips were incubated with 10 μM EdU for a designated time before the fixation with 4% PFA for 20 min ...
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bioRxiv - Cell Biology 2024Quote: ... EdU-labeled cells were visualized using “click” chemistry with an Alexa Fluor® 594-conjugated azide and cell nuclei were stained by DAPI (Life Technologies, P36931). Fluorescent images were captured with a fluorescence microscope (Leica) ...
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bioRxiv - Cell Biology 2024Quote: ... human EGF (10 µg/ml) and bovine insulin (10 µg/ml) in low-attachment plates (Nunclon sphera, Thermo Scientific™ #174932) and maintained at 37°C in a humidified 5% CO2 incubator for 3 days before harvesting ...
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bioRxiv - Cell Biology 2024Quote: ... with 10% fetal bovine serum (Invitrogen, 16000044), 100 units/ml of penicillin and 100 μg of streptomycin (P/S ...
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bioRxiv - Cell Biology 2024Quote: ... 100 units/ml of penicillin and 100 μg of streptomycin (P/S, Gibco,15140-122) at 37 °C in 5% CO2 ...
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bioRxiv - Cell Biology 2024Quote: ... were obtained from American Type Culture Collection (ATCC) and cultured in DMEM medium (Gibco, 12800-017) with 10% fetal bovine serum (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... before SCs were liberated by treating with Collagenase II (100 units per 1 ml) and Dispase (17105-041, Gibco, 1.1 unit per 1 ml) for 30 min ...
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bioRxiv - Cell Biology 2024Quote: ... total RNAs were subjected to polyA selection (Ambion, 61006) followed by library preparation using NEBNext Ultra II RNA Library Preparation Kit (NEB ...
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bioRxiv - Cell Biology 2024Quote: ... Coverslips were washed three times for 10 min in DPBS + 0.05% Tween-20 (DPBS-T) and incubated with 1:1500 donkey anti-rabbit Alexa 594 secondary antibody (Invitrogen) diluted in blocking solution for 1 hr ...
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bioRxiv - Cell Biology 2024Quote: Total RNAs were extracted using TRIzol reagent (Invitrogen) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... in MOPS SDS Running buffer (Invitrogen, NP0001), transferred to PVDF membrane (Thermo scientific ...
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bioRxiv - Cell Biology 2024Quote: ... transferred to PVDF membrane (Thermo scientific, 88518) in NuPAGE transfer buffer (Novex ...
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bioRxiv - Cell Biology 2024Quote: ... and mtHsp70 (Anti-Mouse, Clone: JG1, Invitrogen MA3028) antibody.
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bioRxiv - Cell Biology 2024Quote: Total RNA was extracted using the TRIzol reagent (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... and DAPI (1:100; Invitrogen, Thermo Fisher #D1306).
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bioRxiv - Cell Biology 2024Quote: ... and visualized by enhanced chemiluminescence (Thermo Fisher scientific) using a Bio-Rad imaging system.
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bioRxiv - Cell Biology 2024Quote: ... and Sample Reducing Agent (Invitrogen, NP0009) were added to the samples and heated to 70 °C for 10 min ...
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bioRxiv - Cell Biology 2024Quote: ... Nuclei were counterstained with DAPI (Molecular Probes) then fluorescence was observed using a Nikon TS100 fluorescence microscope.
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bioRxiv - Cell Biology 2024Quote: ... cells were treated with the cell-permeable cross-linking agent BMH (Thermo Scientific) at a concentration of 1 mM for 20 minutes at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... #sc-390378) Phospho-FAKY397 (Anti-Rabbit, 1:1000; ; Invitrogen, (clone 31H5L17) # 700255) ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were transfected with the recombinant pcDNA3.1 plasmid (6 μg/flask) containing mouse Wnt10b cDNA using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... AF488 donkey anti Rabbit IgG and AF647 polyclonal donkey anti-rabbit IgG antibody (all from Invitrogen) were used.
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bioRxiv - Cell Biology 2024Quote: ... LDS sample buffer (Invitrogen, NP0007) and Sample Reducing Agent (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... First-strand cDNA was synthesized from 600 ng of total RNA in the presence of 100 U of SuperScriptTM II Reverse Transcriptase (Invitrogen). Semi-quantitative PCR was performed from cDNAs with Dream Taq (Fermentas ...
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bioRxiv - Cell Biology 2024Quote: ... and the lysate was subjected to Western blot on NuPAGE Novex 3–8% Tris-acetate gradient gels (Invitrogen™ # EA0375BOX), transferred to a nitrocellulose membrane and blotted against OPA1 (Anti-Mouse ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were separated on Nu Page 4–12% Bis-Tris Gel (Invitrogen) in MOPS SDS Running buffer (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... and DAPI (1:100; Invitrogen, Thermo Fisher #D1306).
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bioRxiv - Cell Biology 2024Quote: ... At the end of the incubation the single cell suspensions was supplemented with 10 mL of advanced DMEM/F-12 (Gibco™) media with 10% adult bovine serum ...
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bioRxiv - Cell Biology 2024Quote: ... and 1% Pen/Strep (Invitrogen #15070-063). For all ligand response experiments ...
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bioRxiv - Cell Biology 2024Quote: ... cells were detached from the gel using Versene 1X (Life Technologies) and a reference image of the fluorescent beads was acquired for TFM calculations42 ...
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bioRxiv - Cell Biology 2024Quote: ... and 15 µg/ml fibrinogen-Alexa488 conjugate (F13191, Thermo Fisher) and incubated at room temperature for 1 h ...
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bioRxiv - Cell Biology 2024Quote: ... Transcription levels of genes of interest were assessed by quantitative PCR (qPCR) using SYBR® Green (Thermo Fisher) and a LightCycler® R 96 instrument (Roche Diagnostics ...
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bioRxiv - Cell Biology 2024Quote: ... and mixed with 1,9-dimethyl-methylene blue solution (48μM, Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... in the presence of the DNA dye Hoechst 33342 (#10337G, Invitrogen, 0,5ug/ml) for 3 hours with gentle agitation ...
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bioRxiv - Cell Biology 2024Quote: ... supplemented with 10% v/v foetal bovine serum (FBS; Gibco), 100 U ml penicillin and 100 μg/ml streptomycin ...
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bioRxiv - Cell Biology 2024Quote: ... On the day of electroporation organoids were dissociated into small clumps of 3-5 cells using mechanical pipetting and TrypLE incubation and resuspended in Opti-MEM Reduced Serum Medium (Gibco). 5 μg of the cloned REG1A sgRNA-plasmid ...
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bioRxiv - Cell Biology 2024Quote: ... Secondary antibodies used were purchased from Thermo Fisher Scientific and were as follows ...
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bioRxiv - Cell Biology 2024Quote: ... anti-BAF (custom made by Thermo scientific, 1:1000), anti-Ankle2 (custom made by Thermo Fisher Scientific 1:1000) ...
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bioRxiv - Cell Biology 2024Quote: One pellet per group and donor was digested by 0.1mg/mL proteinase K (Thermo Fisher) in Tris hydrochloride (50mM ...
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bioRxiv - Cell Biology 2024Quote: ... 1% penicillin/streptomycin (Pen Strep, Gibco™ ...
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bioRxiv - Cell Biology 2024Quote: ... Secondary antibodies used were purchased from Thermo Fisher Scientific and were as follows ...