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Citations for Thermo Fisher :
8101 - 8150 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... containing 10% fetal bovine serum (FBS) (Gibco, USA). and 1% penicillin streptomycin (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were evaluated using the Attune flow cytometer (Thermo Fisher Scientific, USA) or the Fortessa flow cytometer (BD Biosciences ...
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bioRxiv - Cancer Biology 2024Quote: ... and treated with red blood cell lysis buffer (Thermo Scientific) prior to staining ...
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bioRxiv - Cancer Biology 2024Quote: ... were coated with 50 μg/mL Poly-Lysine (Thermo Scientific, A3890401) in water for 1h ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 10% BenchMark™ Fetal Bovine Serum (GeminiBio, 100-106) and 1% penicillin-streptomycin (Thermo Scientific, 15140122) with 20 ng/mL murine IL-2 (Peprotech ...
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bioRxiv - Cancer Biology 2024Quote: ... MS data acquisition was performed using a QExactive benchtop orbitrap mass spectrometer equipped with an Ion Max source and a HESI II probe (Thermo Fisher Scientific, San Jose, CA, USA) and was performed in positive and negative ionisation mode in a range of m/z = 70–1000 ...
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bioRxiv - Cancer Biology 2024Quote: ... operated on Vanquish™ Flex UHPLC Systems (Thermo Fisher Scientific, San Jose, CA, USA). Chromatographic separation was achieved using the following conditions ...
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bioRxiv - Cancer Biology 2024Quote: ... CD8 (See Supplementary Table 14) and 12 μg/mL Hoechst (Invitrogen, H3570) in blocking solution for 45 min at RT in the dark ...
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bioRxiv - Cancer Biology 2024Quote: ... plastic flat bottom 96 well plates were coated with 5 μg/mL human fibronectin in PBS (GIBCO, 14080-055) for 1h in 37 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... The remaining red blood cells were lysed by incubation in an ACK lysing buffer (Gibco), and the platelets were eliminated by centrifugation (10 min ...
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bioRxiv - Cancer Biology 2024Quote: ... S1 fraction was washed from the filter using fresh full media supplemented with 100 U/mL collagenase type IV (Life Technologies, 17104019), and 15 mM HEPES (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... Huh7 and HepG2 spheroids were co-cultured with CellTrace Violet (Life Technologies) labeled Peripheral Blood Mononuclear Cells (PBMC) ...
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bioRxiv - Cancer Biology 2024Quote: ... and 15 mM HEPES (Gibco, 1560-080). S1 with collagenase was incubated for 15-30 minutes at 37°C followed by the addition of an equal volume of media and subsequent filtering ...
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bioRxiv - Cancer Biology 2024Quote: ... washed twice with PSB 1X and dissociated with trypsin 0.25%-EDTA (10 min, 37°C, Gibco). Cells were then pelleted by centrifugation ...
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bioRxiv - Cancer Biology 2024Quote: ... diluted 1:1 in full media and 12ug/ml Hoechst (Invitrogen, H3570) or 12 μg/mL Hoechst Hoechst and 0.8 μg/mL PI alone (Thermo Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... for CT26 tumours supplemented with 10% BenchMark™ Fetal Bovine Serum (GeminiBio, 100-106) and 1% penicillin-streptomycin (Thermo Scientific, 15140122) on ice and minced in a 10 cm plate using sterile forceps and scalpels ...
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bioRxiv - Cancer Biology 2024Quote: ... Costar) in 100 µL filtrated 4.5 g/L glucose Dulbecco’s modified Eagle’s Medium (DMEM, Gibco) supplemented with 10 % exosome free FBS for 96 hours ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA was then reverse transcribed using material from ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... and 10 nM MitoTracker Green (ThermoFisher Scientific, M7514), respectively for 30 min at 37 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... or 12 μg/mL Hoechst Hoechst and 0.8 μg/mL PI alone (Thermo Scientific, P3566). After incubation with fluorescent dyes (30 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... coupled to a nanospray Orbitrap Fusion™ Lumos™ Tribrid™ Mass Spectrometer (Thermo Fisher Scientific, California, USA). Each peptide extract was loaded on a 300 μm ID x 5 mm PepMap C18 precolumn (Thermo Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... NanoLC-MS/MS analysis were performed using an Ultimate 3000 RSLC Nano-UPHLC system (Thermo Scientific, USA) coupled to a nanospray Orbitrap Fusion™ Lumos™ Tribrid™ Mass Spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: For flow cytometry analysis of mitochondrial membrane potential (MMP) and Mitochondrial mass T cells were stained with 10 nM MitoTracker Deep Red (ThermoFisher Scientific, M22426) and 10 nM MitoTracker Green (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... Each peptide extract was loaded on a 300 μm ID x 5 mm PepMap C18 precolumn (Thermo Scientific, USA) at a flow rate of 10 μL/min ...
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bioRxiv - Cancer Biology 2024Quote: ... peptides were separated on a 50 cm EasySpray column (75 μm ID, 2 μm C18 beads, 100 Å pore size, ES903, Thermo Fisher Scientific) with a 4-40% linear gradient of solvent B (0.1% formic acid in 80% ACN ...
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bioRxiv - Cancer Biology 2024Quote: ... Staining solution in the side channels was washed twice with PBS + 0.1% Tween 20 (Fisher Scientific, BP337) and replaced with PBS for imaging ...
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bioRxiv - Cancer Biology 2024Quote: ... 1.0 × 106 B16-ova cells (kindly provided by Dr. Debattama Sen, MGH) were resuspended in sterile Ca- and Mg-free PBS (Gibco) and subcutaneously injected into the flank on day 0 ...
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bioRxiv - Cancer Biology 2024Quote: Targeted relative quantification of polar metabolites was standardly performed with TraceFinder 5.1 (Thermo Fisher Scientific, Waltham, MA, USA) using a 10 ppm mass tolerance and referencing an in-house library of chemical standards (230 compounds and 35 isotopically labelled internal controls) ...
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bioRxiv - Cancer Biology 2024Quote: ... fixation and permeabilization using eBioscience™ Intracellular Fixation & Permeabilization Buffer Set (Thermo Fisher, 88-8824-00), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: For acute versus chronic proliferation measurements total cell number for acute and chronic was counted at day 0 and every 2-3 days until day 10 using Countess automated cell counter (Thermo Fisher). For Incucyte® proliferation assay plastic flat bottom 96 well plates were coated with 5 μg/mL human fibronectin in PBS for 1h in 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... 2.0 × 106 CT26-GFP cells (Kindly provided Dr. Moshe Sade-Feldman, MGH) were resuspended in sterile Ca- and Mg-free PBS (Gibco) and subcutaneously injected into the flank on day 0 ...
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bioRxiv - Cancer Biology 2024Quote: ... and HCT116 (kindly provided by Dr. Vogelstein’s laboratory) cell line was cultured in RPMI-1640 medium (GIBCO). All mediums were supplemented with 10% fetal bovine serum (GIBCO ...
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bioRxiv - Cancer Biology 2024Quote: ... Lysates were boiled in LDS loading buffer with 0.1M DTT and run on 4-12% precast Bis-Tris gels (NP0336BOX, Thermo Fisher Scientific, Waltham, MA). Proteins were transferred using an iBlot2 transfer device (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... Pulldown of biotin-labeled DNA was initiated by separating 10 µL of 10 mg/mL Dynabeads MyOne Streptavidin T1 beads (Life Technologies, 65602) on a magnetic stand ...
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bioRxiv - Cancer Biology 2024Quote: ... edu staining was performed with the Click-iT Plua EdU Alexa Fluor 647 Imaging kit (Invitrogen, cat:C10640).
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bioRxiv - Cancer Biology 2024Quote: ... using RNAiMax Lipofectamine Transfection Reagent (Invitrogen) or Lipofectamine 3000 Transfection Reagent (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... or Lipofectamine 3000 Transfection Reagent (Invitrogen) to transfect siRNA or plasmid ...
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bioRxiv - Cancer Biology 2024Quote: ... which were cultured in DMEM medium (GIBCO), and HCT116 (kindly provided by Dr ...
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bioRxiv - Cancer Biology 2024Quote: ... CD81 (Clone 1.3.3.22, Thermo Fisher Scientific, Waltham, MA, 1:20), Goat anti-mouse AF647 (ab150115 ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA was precipitated in 75% EtOH with 1μl glycoblue (Ambion 9516) overnight at -20 °C.
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bioRxiv - Cancer Biology 2024Quote: Cells and EVs were lysed in RIPA buffer supplemented with protease/phosphatase inhibitors (#78440, Thermo Fisher Scientific, Waltham, MA) and quantified with DC Protein Assay (#500111 ...
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bioRxiv - Cancer Biology 2024Quote: Cells were grown in 8 well chambered coverglass (#155409, Thermo Fisher Scientific, Waltham, MA) and imaged with an SP8 white laser confocal (Leica Biosystems ...
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bioRxiv - Cancer Biology 2024Quote: ... Filtrate was evaporated to dryness using Speed Vac (Thermo Scientific, MA, USA). The dried extract was dissolved in 50 µl of sample buffer (6.5:2.5:1 Acetonitrile ...
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bioRxiv - Cancer Biology 2024Quote: Transfection reaction was carried out in Opti-MEM medium (GIBCO) using RNAiMax Lipofectamine Transfection Reagent (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... The wells were washed once with 100 µL of phosphate buffered saline (PBS) (Gibco, #10010-023) and all liquid was removed from the wells and allowed to dry fully at RT ...
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bioRxiv - Cancer Biology 2024Quote: ... in 1× HBSS (Gibco) and incubated for 30 min at 37 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... Mycoplasma and cell line genotyping were performed once a fortnight and every month respectively at the University of Michigan Sequencing Core using Profiler Plus (Applied Biosystems). Results from these were compared with corresponding short tandem repeat profiles in the ATCC database to authenticate their identity.
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bioRxiv - Cancer Biology 2024Quote: ... 20ng of cDNA was used for each polymerase chain reaction (PCR) using the FAST SYBR Green Universal Master Mix (ThermoFisher Scientific), and every sample was quantified in triplicates ...
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bioRxiv - Cancer Biology 2024Quote: ... nuclear or whole cell protein extracts were obtained from cells using NE-PER nuclear extraction kit (Thermo Scientific, 78835) or RIPA buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were loaded and run on 4-12% Bis-Tris Gradient Gel (Fisher Scientific WG1403BOX) and transferred onto nitrocellulose membrane (Bio-Rad 1704271) ...