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Citations for Thermo Fisher :
7801 - 7850 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... HORSERADISH Peroxidase (HRP) Conjugated (Goat Polyclonal, Thermo Scientific, 31470). Different antigen retrieval reagent conditions ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were harvested and stained with 1mM SYTOX Blue (Invitrogen S34857) for the exclusion of dead cells ...
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bioRxiv - Cancer Biology 2024Quote: ... This suspension was characterized by the measurement of the absorbance spectrum (Evolution 220, Thermo Scientific) and of the luminescence map (SI figure 1(A)) ...
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bioRxiv - Cancer Biology 2024Quote: ... and 1% penicillin plus streptomycin (P/S) (Gibco 15140-122). Mouse tumor-derived cell lines were derived as previously described ...
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bioRxiv - Cancer Biology 2024Quote: ... the sections were treated with goat-anti-mouse IgG Alexa Fluor 488 and goat-anti-mouse IgG Alexa Fluor 555 for 1 hour (both from Thermo Fisher Scientific, MA, USA). The staining procedure was conducted using the Antifade Reagent with DAPI (Cell Signaling Technology ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein concentrations were measured using a Nanodrop 2000 system (Thermo Fisher Scientific, MA, USA). Proteins were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (Bio-Rad Laboratories ...
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bioRxiv - Cancer Biology 2024Quote: ... The complex or Cas9 only (control wild type cells) was mixed with a non-specific oligonucleotide (IDT) and electroporated into 5×105 ARP1 cells using a NEON (ThermoFisher Scientific, Waltham, MA) apparatus at a setting of a 20 msec pulse at 1600 Volts ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was extracted using TRIzol reagent (ThermoFisher Scientific) and cDNA was synthesized from 1μg RNA using iSCRIPT as per the manufacturer’s instructions (Biorad ...
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bioRxiv - Cancer Biology 2024Quote: ... using Lipofectamine P3000 (ThermoFisher Scientific). Viral supernatant was collected ...
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bioRxiv - Cancer Biology 2024Quote: ... using Lipofectamine P3000 (ThermoFisher Scientific).
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 10% fetal bovine serum (FBS) (Gibco) and 1% penicillin-streptomycin (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... final concentration: 5 µM) and 4 µL of 1 mg/mL Hoechst 33342 dye (1:500 dilution) (Life technologies, Eugene, OR, USA) and resuspended thoroughly using a low binding pipette tip ...
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bioRxiv - Cancer Biology 2024Quote: ... using Lipofectamine P3000 (ThermoFisher Scientific). Viral supernatant was collected ...
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bioRxiv - Cancer Biology 2024Quote: H1975 and H1975-GFP cells were pre-labeled with the CellTracker™ Green CMFDA dye (Catalog #C7025, Thermo Fisher Scientific, Eugene, USA) prior to spike-in experiments to facilitate detection and enumeration ...
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bioRxiv - Cancer Biology 2024Quote: ... mouse MHC-I FITC conjugated H2-Dd/H-2Kd clone 34-1-2S (Invitrogen #11-5998-82), mouse MHC-I PE conjugated H-2Kb clone AF6-88.5.5.3 (Invitrogen #12-5958-82).
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bioRxiv - Cancer Biology 2024Quote: ... The cell pellet was then resuspended in 2 mL RPMI 1640 + GlutaMAX™ (Gibco) containing 2 µL of 5 mM the CellTracker™ Green CMFDA dye stock solution (1:1000 dilution ...
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bioRxiv - Cancer Biology 2024Quote: Lipofectamine RNAiMAX Reagent (Invitrogen, Carlsbad, CA, USA) and mimic miR200c (Eurofins Genomics ...
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bioRxiv - Cancer Biology 2024Quote: ... BCA protein analysis (ThermoFisher Scientific) was used to determine equal concentrations of protein for each sample lysate ...
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bioRxiv - Cancer Biology 2024Quote: ... Cultures were maintained in 1% Antibiotic-Antimycotic (Life Technologies, Carlsbad California ...
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bioRxiv - Cancer Biology 2024Quote: ... permeabilized and the nuclei were stained using a solution with a final concentration of 2% PFA (Thermo Scientific; Waltham, MA, USA), 0.01% Triton™ (Roche ...
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bioRxiv - Cancer Biology 2024Quote: ... This involved initial assessment of RNA purity using Nanodrop2000 (Thermo Scientific; Waltham, MA, USA), agarose gel electrophoresis for RNA integrity and potential contamination ...
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bioRxiv - Cancer Biology 2024Quote: ... coli (C737303; Invitrogen) cultures and verified with DNA digestion and gel electrophoresis before clonal expansion and purification with QIAGEN Plasmid Maxi Kit was performed (12163 ...
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bioRxiv - Cancer Biology 2024Quote: ... 1X lipopolysaccharide (LPS; Invitrogen; Carlsbad, CA) and 1X PMA/Ionomycin (BioLegend ...
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bioRxiv - Cancer Biology 2024Quote: ... (Invitrogen, Waltham, MA, USA), as described previously 16,17,19 by studying the CFSE/CTV fluorescence intensity decay rate overtime as measured by flow cytometry ...
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bioRxiv - Cancer Biology 2024Quote: ... Their viability was assessed using PI staining (Thermo Scientific; Waltham, MA, USA). Cell staining was performed with the method as recommended by the manufacturer’s manual ...
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bioRxiv - Cancer Biology 2024Quote: The cytotoxic effects of TMZ at a dosage of 2mM was further assessed by using the fluorescence-based CyQUANT Cell Proliferation assay (Thermo Fisher Scientific Waltham, MA, USA Cat#C7026). The assay measures proliferation and membrane integrity ...
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bioRxiv - Cancer Biology 2024Quote: ... Quantitative PCR of RT products (qRT-PCR) was conducted using VeriQuest SYBR Green qPCR Master Mix (Affymetrix). Peptidylprolyl isomerase B (Ppib ...
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bioRxiv - Cancer Biology 2024Quote: ... and 0.1% DAPI (Thermo Scientific; Waltham, MA, USA). The Gen5 Image software on the Cytation™ 3 Cell Imaging Multi-Mode Reader was utilized to quantify spheres that exhibited a circularity exceeding 0.15 and measured between 50 to 500 μm in diameter.
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bioRxiv - Cancer Biology 2024Quote: ... and a goat anti-rabbit AF488 conjugated secondary antibody (1:1000; Cat#11008 Invitrogen, Waltham, MA, USA).
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bioRxiv - Cancer Biology 2024Quote: The cells were cultured at 37□°C in the presence of 5% CO2 in DMEM (Gibco, New York, USA Cat#11965092) and supplemented with 10% FBS (Avantor ...
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bioRxiv - Cancer Biology 2024Quote: ... Assessment of library effective concentration and RNA quality were performed using Agilent 2100 Bioanalyzer and Qubit2.0 (Thermo Scientific; Waltham, MA, USA). Finally ...
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bioRxiv - Cancer Biology 2024Quote: ... and 1% β-mercaptoethanol (ThermoFisher Scientific; Waltham, MA). For all cell lines ...
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bioRxiv - Cancer Biology 2024Quote: ... and supplemented with 10% FBS (Avantor, Pennsylvania, USA Cat#89510186) and 1% Antibiotic-Antimycotic (Life Technologies, Waltham, MA, USA, Cat# 15240062). Upon reaching 90% confluency ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were either left unstimulated or stimulated with 1X eBioscience Cell Stimulation Cocktail (500X, Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... in 100uL complete RPMI (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... qPCR was performed using TaqMan gene expression Master Mix (Applied Biosystems, Waltham, MA, #4369016) on a StepOne Plus Real-Time PCR system (Applied Biosystems).
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bioRxiv - Cancer Biology 2024Quote: ... Anti-VIM (#MA5-11883) was from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Cancer Biology 2024Quote: ... 25 µL of supernatant was analyzed using Cytokine 35-Plex Human Panel for the Luminex™ platform (Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: siRNAs were transfected using the Lipofectamine RNAiMAX (Life Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... mitochondrial or OMM fractions (both obtained as described in “Mitochondrial fractionation” of the Methods section) were precleared with 20 μL of streptavidin acrylamide beads (Thermo Fisher Scientific) in the binding buffer containing 20mM Tris pH 8.0 ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by 1 h incubation at 25 °C in a light-tight box with Streptavidin-Cy3 (1 μg/mL, Thermo Fisher Scientific, 10768023) and fluorescent secondary antibody Alexa Fluor 488 donkey anti-mouse (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... Immunolabeling were visualized the next day with fluorescent secondary antibody Alexa Fluor 488 goat anti-rabbit (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... 4 µL was reverse transcribed using the RevertAidH Minus First (Thermo Fisher Scientific EP0452) following the manufacturer’s indication ...
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bioRxiv - Cancer Biology 2024Quote: ... and DNase I (Thermo Fisher Scientific EN0521) for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were labeled with 100 nM MTG (Thermo Fisher Scientific, 11589106) and 200 nM TMRE (Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... and fluorescent secondary antibody Alexa Fluor 488 donkey anti-mouse (Invitrogen, A21202, 1:800 in 1% BSA/1X PBS) or Alexa Fluor 647 goat anti-rabbit (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... Plasmids and RLuc-RNA reporters were transfected using the Lipofectamine 3000 (Life Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: All RNAs were obtained using the MEGAscript Kit (Invitrogen AM1333) as per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Labtech and MARS Clariostar Analysis Software v.3.31) using the RevertAidH Minus First (Thermo Fisher Scientific EP0452) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... The ChIP library was validated using Agilent TapeStation and quantified using Qubit 2.0 Fluorometer as well as real time PCR (Applied Biosystems). The sequencing libraries were multiplexed and clustered on one lane of a flowcell ...