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Citations for Thermo Fisher :
7701 - 7750 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Increased dosage of wild-type KRAS protein drives KRAS-mutant lung tumorigenesis and drug resistancebioRxiv - Cancer Biology 2024Quote: ... Protein concentration was quantified using a Pierce BCA protein assay kit (ThermoFisher). For immunoblotting ...
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bioRxiv - Cancer Biology 2024Quote: ... coupled with a Q Exactive HF Orbitrap MS (Thermo Fisher) in negative ion mode and metabolites were eluted within a 45 min gradient (buffer A ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein gel (Invitrogen). Antibodies were added according to the manufacturers recommended conditions ...
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bioRxiv - Cancer Biology 2024Quote: ... 100LU/mL penicillin and 100Lµg/mL streptomycin (Pen-Strep, Cat. No. 15140122, Thermo Fisher). The stocks of NCI SCLC cell lines that we used were STR profiled by NIH or the ATCC ...
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Increased dosage of wild-type KRAS protein drives KRAS-mutant lung tumorigenesis and drug resistancebioRxiv - Cancer Biology 2024Quote: ... Peptides were eluted over a 44-min gradient and analysed with an Orbitrap EclipseTM TribridTM instrument (Thermo Fisher) with FAIMS ProTM Interface (ThermoFisher ...
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bioRxiv - Cancer Biology 2024Quote: ... The miR-539-5p mimic or NC was cotransfected with the wild-type or mutant plasmid using lipofectamine 3000 (Invitrogen). Luciferase intensity was recorded 48 h after transfection using the dual-luciferase reporter assay system (Promega ...
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bioRxiv - Cancer Biology 2024Quote: ... 5 μL Quantitect SYBR Green PCR Kit (Thermo Fisher), and RNA/Nuclease free water ...
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bioRxiv - Cell Biology 2024Quote: ... the fixed cells were incubated in a blocking solution containing 5% goat serum (Gibco) and 0.3% Triton™ X-100 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... Prevention of mycoplasma contamination was done by adding Antibiotic-Antimycotic (Thermo Fisher Scientific, US) during cell culture ...
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bioRxiv - Cell Biology 2024Quote: ... in DMEM (Invitrogen, 52100-047) containing 10% heat-inactivated FBS (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... containing 10% heat-inactivated FBS (Invitrogen, 10437028) and 100 U/ml of penicillin/streptomycin (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were incubated in DAPI for nuclei staining (1μg/mL, Thermo Scientific) for 10 min ...
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bioRxiv - Cell Biology 2024Quote: ... 0.1 mM nonessential aminoacids (NEAA, Invitrogen), 50 U/mL streptomycin (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... 400,000 undifferentiated hPSCs were resuspended in Essential 6 medium (Gibco) supplemented with 500 nM LDN193189 and 10μM SB431542 ...
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bioRxiv - Cell Biology 2024Quote: ... Alexa-488 or 555 or 647 were used as secondary antibodies (Invitrogen). Nuclei were counterstained with DAPI.
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bioRxiv - Cancer Biology 2024Quote: ... and the resulting peptides were analyzed by LC-MS/MS on a Q-Exactive MS instrument (Thermo Scientific). Peptides were separated by nano-LC using an UltiMate 3000 high performance liquid chromatography and autosampler system (Dionex ...
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bioRxiv - Cancer Biology 2024Quote: ... beads were incubated in kinase reaction buffer supplemented with 100 ng recombinant CAMK2D (Invitrogen, cat. PV3373), 100 µM recombinant calmodulin (Enzo ...
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bioRxiv - Cancer Biology 2024Quote: ... 1:100 Halt Protease Inhibitor Cocktail (Thermo Scientific, 78430), and 10mM sodium butyrate] ...
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bioRxiv - Cancer Biology 2024Quote: ... The PCR product was digested with Esp3I (BsmBI) (Fisher Scientific, cat#FERFD0454) and ligated into the digested lentiCRISPRv2 backbone ...
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bioRxiv - Cancer Biology 2024Quote: ... and passaged twice a week using Trypsin-EDTA (0.25%) (Life Technologies). Cells were tested for mycoplasma contamination using the MycoAlert Mycoplasma Detection Kit (Lonza ...
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bioRxiv - Cancer Biology 2024Quote: ... with 10% fetal bovine serum (Life Technologies), 1% penicillin-streptomycin ...
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bioRxiv - Cancer Biology 2024Quote: ... Lipofectamine was used as a transfection reagent (Life Technologies). OPTIMEM (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... proteins were visualized on film after incubation with SuperSignal West Pico PLUS Chemiluminescent Substrate (ThermoFisher, Waltham, MA).
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bioRxiv - Cancer Biology 2024Quote: ... Western blot signals were detected by using SuperSignal West Pico Chemiluminescent substrate and visualized using a iBright imaging system (Model CL1500, ThermoFisher). Primary antibodies used in this study include NeuroD1 (1:1000 ...
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bioRxiv - Cancer Biology 2024Quote: ... 50 µl Human Epidermal Growth Factor (hEGF; 500 µg/ml, Invitrogen), 50 µl human Fibroblast Growth Factor-10 (hFGF-10 ...
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bioRxiv - Cancer Biology 2024Quote: ... 1% L-glutamine (200 nM, Gibco), 1% MEM nonessential amino acids (Sigma Aldrich) ...
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bioRxiv - Cancer Biology 2024Quote: HeLa cell lines were cultured in DMEM/High glucose (Thermo Fisher Scientific, Inc., Waltham, MA, USA), supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 ml B-27 supplement (50x, Gibco), 5 ml R-spondin ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein was produced from IL-2 and IL-12 expressing stable lines during one-week culture in serum-free media (Freestyle 293, Invitrogen) and purified with HisPur Ni-NTA affinity resin (ThermoFisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... PCR amplification was performed using a Step One Plus real time PCR system (Applied Biosystems, Warrington, UK) with TOPrealTM qPCR 2X PreMIX (Enzynomics) ...
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bioRxiv - Cancer Biology 2024Quote: 293FT cells or Lenti-X 293T cells (carrying pLVX lenti-backbone) were transfected with the plasmid using the calcium phosphate transfection kit (Thermo Fisher). Following the transfection ...
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bioRxiv - Cancer Biology 2024Quote: ... Coverslips were mounted onto slides using Prolong Gold anti-fade mounting medium with DAPI (Invitrogen). Images were acquired with a microscope (Zeiss LSM880 Airyscan microscope ...
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bioRxiv - Cancer Biology 2024Quote: ... in 99 ml of DMEM (Gibco). Human Organoid Growth Medium (HOGM) ...
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bioRxiv - Cancer Biology 2024Quote: ... 30% Bovine Serum Albumin (BSA) diluted in Advanced DMEM/F12 medium (AdDMEM/F12, Gibco). For initial seeding ...
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bioRxiv - Cancer Biology 2024Quote: Murine Organoid Growth Medium (MOGM): 50 ml of MOGM contained 5 µl murine Epidermal Growth Factor (mEGF; 500 µg/mll, Invitrogen), 50 µl murine Fibroblast Growth Factor-10 (mFGF-10 ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific, Inc., Waltham, MA, USA) and 1% penicillin-streptomycin (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... and permeabilized with PBS (Gibco) containing 0.5% Triton X-100 (Sigma Aldrich) ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 ml B-27 supplement (50x, Gibco), 100 µl Primocin (50 mg/ml ...
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bioRxiv - Cancer Biology 2024Quote: ... and 1 ml of 10% FCS (Gibco) in 99 ml of DMEM (Gibco) ...
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bioRxiv - Cancer Biology 2024Quote: ... and purified with HisPur Ni-NTA affinity resin (ThermoFisher Scientific). Protein was analyzed by size exclusion chromatography (Superdex 200 Increase 10/300 GL column ...
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bioRxiv - Cancer Biology 2024Quote: ... and 1% penicillin-streptomycin (10,000 U/mL, Gibco) in 5% CO2 at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... by using a T3 enzyme-linked immunosorbent assay kit (#EIAT3C, ThermoFisher), or analyzing tumor cell differentiation by immunohistochemistry ...
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bioRxiv - Cancer Biology 2024Quote: ... by using a T3 enzyme-linked immunosorbent assay kit (#EIAT3C, ThermoFisher). The blood was extracted from mice after the treatment ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was extracted from cells or tissues using TRIzol Reagent (Invitrogen). The RNA was then reversely transcribed to cDNA using Primescript RT reagent Kit Thermo ...
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bioRxiv - Cancer Biology 2024Quote: ... 500 ng of RNA from each sample was used for cDNA synthesis using High-capacity RNA-to-cDNA kit (#4387406, Thermo Fisher Scientific). qPCR mixture was prepared using PowerUp™ SYBR™ Green Master Mix for qPCR (#A25741 ...
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bioRxiv - Cancer Biology 2024Quote: ... Dynabeads™ Protein G (ThermoFisher, 10003D) were conjugated with antibodies by incubating overnight with rotation at 4°C and washed two times with ice cold lysis buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... for mammalian cells (# L3224, Thermo Fisher Scientific), and incubated at 37°C for 30 min ...
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bioRxiv - Cancer Biology 2024Quote: ... spRNA-seq slices were transferred on library preparation spatial transcriptomics slides and IF slices were transferred on SuperFrost® Plus microscope slides (Thermo scientific, j1800amnz). Slices used for single nuclei sequencing and genotyping were 20µm-thick and collected in Eppendorf® DNA LoBind tubes (Eppendorf ...
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bioRxiv - Cancer Biology 2024Quote: ... containing 500µL of Dulbecco’s phosphate-buffered saline (Gibco™, 14040117). The mix was homogenized and transferred to a new DNA LoBind tube after adding 500µL of DPBS ...
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bioRxiv - Cancer Biology 2024Quote: Enzymatically dissociated cells were plated on to Shandon™ Multi-Spot Slides (#9991090, ThermoFisher Scientific) at a concentration of 30000 cells / 3µL and allowed to air dry at RT ...