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Citations for Thermo Fisher :
8001 - 8050 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... pCS2-based expression plasmids were transfected into HeLaK cells with Lipofectamine 2000 (Invitrogen) according to manufacturer’s protocol and into Hek293T cells using a calcium phosphate-based method ...
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bioRxiv - Molecular Biology 2024Quote: ... 396400 (Life Technologies), mouse α-LaminA/C Cat ...
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bioRxiv - Molecular Biology 2024Quote: ... CD68 (Invitrogen Waltham, MA), and CD163 (BD Pharmingen San Diego ...
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bioRxiv - Molecular Biology 2024Quote: ... siRNA was transfected with lipofectamine RNAiMAX (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... were used to perform real-time PCR on a 7900HT Fast Real-Time PCR System (Applied Biosystems, Waltham, MA). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH ...
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An endothelial SOX18-mevalonate pathway axis enables repurposing of statins for infantile hemangiomabioRxiv - Molecular Biology 2024Quote: ... coupled to a Vanquish UHPLC system (Thermo Fisher, Waltham, USA) using the transition from precursor mass of m/z 369.3516 (MS1 ...
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bioRxiv - Molecular Biology 2024Quote: ... On day 13 adherent macrophages were harvested using Trypsin-EDTA (0.25%) (ThermoFisher Waltham, MA). Macrophages were characterized by cytospin and flow cytometry under basal conditions and following LPS (Sigma-Aldrich St ...
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bioRxiv - Molecular Biology 2024Quote: ... and additionally purified of DNA contamination using DNA-free kit (Thermo Fisher). 2μg of RNA was reverse-transcribed using High Capacity cDNA Reverse Transcription kit (Applied Biosystems ...
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An endothelial SOX18-mevalonate pathway axis enables repurposing of statins for infantile hemangiomabioRxiv - Molecular Biology 2024Quote: ... Amplification was carried out in a QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher Scientific). A relative standard curve for each gene amplification was generated to determine the amplification efficiency ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed again with PBS and mounted onto glass slides using ProLong Gold Antifade Mountant (Invitrogen) and left to dry overnight ...
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bioRxiv - Molecular Biology 2024Quote: ... and a Qubit fluorometer (InVitrogen) for DNA concentrations and size distributions ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.5 mM L-glutamine (GIBCO 25030), 0.5X non-essential amino acids (GIBCO 11140) ...
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bioRxiv - Molecular Biology 2024Quote: ... was supplemented with 5 mL N2 supplement (GIBCO 17502048), 10 mL B27 supplement (GIBCO 17504044) ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 μL of Rapid Ligation Buffer (Thermo Fisher Scientific, K1422), 0.1 μL of T7 Ligase (New England Biolabs ...
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bioRxiv - Molecular Biology 2024Quote: ... 10 mL B27 supplement (GIBCO 17504044), 0.5 mM L-glutamine (GIBCO 25030) ...
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bioRxiv - Molecular Biology 2024Quote: ... supplemented with 15 % ESC-qualified fetal bovine serum (Thermo Fisher Scientific), 1x non-essential amino acids (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: ... 100 U/mL Penicillin-Streptomycin (GIBCO 15140), 0.1 mM b-mercaptoethanol (Sigma) ...
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bioRxiv - Molecular Biology 2024Quote: ... We then extracted nucleic acid using Dynabeads MyOne Silane beads (Thermo Fisher), treated samples with TURBO DNase (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2024Quote: ... and a Qubit fluorometer (InVitrogen) for DNA concentrations and size distributions ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were subjected to a 5-minute wash with buffer (0.35% Triton-X, Thermo Fisher PRH5142, in PBS) and permeabilized with 0.5% Triton-X in PBS for 1 hour ...
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bioRxiv - Molecular Biology 2024Quote: ... 10 uL Protein A Dynabeads (Thermofisher 10002D) were added to DNA Lo-Bind tubes and washed three times on a magnetic rack with 1 mL mg/mL BSA ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were transfected using either Lipofectamine 3000 according to manufacturer’s protocol (Invitrogen).
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bioRxiv - Molecular Biology 2024Quote: ... and 100 U/ml penicillin-streptomycin (all Gibco). SK-BR-3 HER2-knockout (SK-BR-3 KO ...
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bioRxiv - Molecular Biology 2024Quote: ... Gels were then stained by shaking for 20 minutes in 50 mL TBE and 10,000x SYBR-green (Fisher Scientific, E33075) and washed with water ...
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bioRxiv - Molecular Biology 2024Quote: ... U2OS cells were plated in 96-well glass-bottom plates (ThermoFisher Cat. No NC0536760) at 30-50% confluency and transduced with lentivirus for 2-3 days prior to live-cell imaging experiments.
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bioRxiv - Molecular Biology 2024Quote: ... The secondary antibodies (AlexaFluor 647 goat-anti-rabbit Thermo Fisher A-21245, 1:1000 dilution) were prepared in blocking buffer and incubated for 2 hours at 4 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... and incubated in OptiMEM (ThermoFisher Cat No. 31985062). Approximately 48 hours after transfection ...
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bioRxiv - Molecular Biology 2024Quote: ... coverslips were mounted on a drop of ProLong™ Gold antifade reagent with DAPI (Invitrogen). Confocal images were taken on a LSM700 Zeiss Confocal Laser Scanning Microscope using a 63x Oil objective.
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bioRxiv - Molecular Biology 2024Quote: ... were cultured in DMEM (GIBCO, 11995065) supplemented with 10% FBS (Atlanta Biological ...
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bioRxiv - Molecular Biology 2024Quote: ... in MOPS buffer at 100 V for 20 min and subsequently at 150V for 190 min on ice along with PageRuler™ Plus Prestained Protein Ladder (Thermo Scientific. Proteins were transferred to a 0.2 µm nitrocellulose membrane by Trans-Blot Turbo™ Transfer system (Bio-Rad) ...
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bioRxiv - Cell Biology 2024Quote: ... ovarioles were stained with CellMask Orange Plasma membrane Stain (ThermoFisher; diluted 1:10 000 in culture medium) for 15 minutes and washed with ex vivo culture medium before imaging ...
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bioRxiv - Cell Biology 2024Quote: ... Each EM grid was applied with 4 µl sample and vitrified by plugging into liquid ethane using the Vitrobot Mark IV system (Thermo Fisher Scientific). The cryo-EM samples were examined using an FEI Tecnai Arctica 200 kV transmission electron microscope ...
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bioRxiv - Developmental Biology 2024Quote: ... and kept in DMEM (Thermo Fisher, cat. # 31966-047) supplemented with 10% fetal bovine serum (Gibco ...
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bioRxiv - Developmental Biology 2024Quote: ... supplemented with 10% fetal bovine serum (Gibco, cat. # 10270106) and 1% penicillin/streptomycin ...
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bioRxiv - Developmental Biology 2024Quote: ... DAPI was used to counterstain nuclei and the Click-it reaction kit used to reveal EdU (Invitrogen, cat. # C10340).
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bioRxiv - Developmental Biology 2024Quote: ... IVF 35mm dishes (Nunc, Thermo Scientific; 150255) were used for mouse embryo cultures ...
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bioRxiv - Developmental Biology 2024Quote: ... while 60 mm IVF dishes (Corning C353802 from Thermo Scientific; 150260) for human embryo cultures ...
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bioRxiv - Developmental Biology 2024Quote: ... and embryos were dissociated into single cells with TrypLE Express (12604-013, Gibco, Life Technologies) collected using fine glass capillaries as we previously described85 ...
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bioRxiv - Developmental Biology 2024Quote: ... IVF 35mm dishes (Nunc, Thermo Scientific; 150255) were used for mouse embryo cultures ...
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bioRxiv - Neuroscience 2024Quote: ... Cryostat sections were made at 20 µm intervals and mounted onto Superfrost Plus slides (Fisher Scientific Cat# 1255015), with knockout and littermate control tissues on the same slide for comparison.
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bioRxiv - Microbiology 2024Quote: ... The final PCR product was ligated with linear pCR-Blunt using a Zero Blunt PCR cloning kit (Life technologies) in DH5ɑ E ...
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bioRxiv - Microbiology 2024Quote: ... The clarified lysate was transferred to a 15 mL column containing 3 mL Ni-NTA Agarose affinity resin equilibrated with buffer A at 4 °C (Invitrogen). The column was washed using a gradient of Buffer A containing 20 mM ...
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bioRxiv - Microbiology 2024Quote: ... and resuspended in 1 ml 1X HN with 100 μl Halt Protease Inhibitor Cocktail (Thermo Fisher Scientific). Spirochetes were lysed by sonication and spun at 125,000 x g to collect the soluble and membrane components of the lysate.
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bioRxiv - Molecular Biology 2024Quote: ... quantity and integrity were assessed with a NanoDrop (Thermo Fisher Scientific) and a 4200 TapeStation System (Agilent Technologies ...
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bioRxiv - Microbiology 2024Quote: ... RNA was isolated using TRIzol (Thermo Fisher Scientific) exactly as described previously (Melamed et al. ...
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bioRxiv - Molecular Biology 2024Quote: ... supplemented with 50 U/mL penicillin (Gibco), 50 μg/mL streptomycin (Gibco ...
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bioRxiv - Neuroscience 2024Quote: ... 1% penicillin/streptomycin (Thermo Fisher Scientific, Cat# 15070063), 1% GlutaMAX® (United States Biological ...
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bioRxiv - Molecular Biology 2024Quote: ... 50 μg/mL streptomycin (Gibco) and 2 mM L-glutamine (Gibco ...
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bioRxiv - Microbiology 2024Quote: ... and transferred to a nitrocellulose membrane (Thermo Fisher Scientific). Membranes were blocked in 1X TBST containing 5% milk and probed with a 1:2,000 dilution of monoclonal α-FLAG-HRP (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2024Quote: ... Membranes were stripped for 15 min with Restore Immunoblot Blot Stripping Buffer (Thermo Fisher Scientific).