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Citations for Thermo Fisher :
7651 - 7700 of 9391 citations for Mumps Virus Nucleoprotein Strain L Zagreb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... 2.5 µL of a ladder reverse transcription mixture were added consisting of 1.0 µL of 5x First strand Buffer (Invitrogen), 0.25 µL of 0.1M DTT ...
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bioRxiv - Molecular Biology 2024Quote: Global translation rates were measured using Click-iT AHA (L-Azidohomoalanine) Alexa Fluor 488 Protein Synthesis HCS assay kit as per manufacturer’s instructions (Invitrogen). Briefly ...
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bioRxiv - Immunology 2024Quote: ... were cultured in chocolate agar plates at 37 °C in AnaeroJar 2.5 L Jar System (OXOID) and using AnaeroGen 2.5L (Thermo Scientific) to generate an anaerobic atmosphere.
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bioRxiv - Molecular Biology 2024Quote: ... The GD25 cell line (Wennerberg et al., 1996) was cultured at 370C in DMEM supplemented with L-glutamine (Gibco), 10% FBS (Gibco) ...
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bioRxiv - Molecular Biology 2024Quote: ... The 293T cell line (DuBridge et al., 1987) was cultured at 370C in DMEM supplemented with L-glutamine (Gibco), 10% FBS (Gibco) ...
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Cellular sialoglycans are differentially required for endosomal and cell-surface entry of SARS-CoV-2bioRxiv - Microbiology 2024Quote: ... a secondary Alexa Fluor 647 conjugated goat anti-human IgG (H+L) antibody (Invitrogen #A-21445; 10 μg/mL) was used.
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bioRxiv - Biochemistry 2024Quote: ... Dissociated cells were grown in neurobasal medium supplemented with L-glutamine and B27 supplement (A3582801, Life Technologies, Dreieich, Germany) with an exchange of 50% of the medium after 6 days in culture ...
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bioRxiv - Neuroscience 2024Quote: ... The following secondary antibodies were used: goat anti-mouse IgG (H+L) cross-adsorbed Alexa Fluor 546 (RRID: AB_2534071, A-11003, ThermoFisher), goat anti-mouse IgG (H+L ...
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bioRxiv - Microbiology 2024Quote: ... between 5 and 50 μL of cell sample was dropped onto either poly-l-lysine coated chambered slides (Nunc Lab-Tek 8-Well chambered slides ...
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bioRxiv - Immunology 2024Quote: ... in presence of 0.1 μM [4Y]-MBP peptide in a final volume of 200 μl RPMI1640 + L-glutamine (GIBCO) containing 10% FCS and 1% penicillin/streptomycin (Life Technologies) ...
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bioRxiv - Microbiology 2024Quote: ... followed by cross-absorbed goat anti-rabbit IgG (H\&\L) conjugated to either AlexaFluor 488 or 594 fluorophores (Invitrogen).
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bioRxiv - Microbiology 2020Quote: ... 1 μg of pTM1-L and 2 μg of pCI-T7opt were re-suspended in 250 μl of Opti-MEM (Gibco). The plasmid cocktail was then gently mixed with 30 μL of TransIT LT1 transfection reagent (Mirus) ...
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bioRxiv - Biophysics 2021Quote: ... cells were incubated for 2 hours with secondary antibodies: 488/568/633 IgG (H+L) and/or Alexa Flour 568/647 phalloidin (Invitrogen) for 1 hour ...
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bioRxiv - Cancer Biology 2021Quote: ... The peptides were first trapped for 1 minute at 30 µL/min with 100% buffer A on a trap (0.3 x 5mm with PepMap C18, 5μm - 100Å Thermo Fisher Scientific); after the trapping peptides were separated by a 50-cm analytical column packed with C18 beads (Poroshell 120 EC-C18 ...
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bioRxiv - Cancer Biology 2021Quote: ... Phenol red free DMEM with high glucose and L-glutamine (21063-045) for fluorescence imaging was purchased from Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... and HeLa cell lines were cultured at 37°C and 5% CO2 in DMEM (Dulbecco’s Modified Eagle Medium) containing high glucose (4.5 g/L; Thermo Fisher Scientific) with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2020Quote: ... Rabbit anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody with Alexa Fluor 594 (A27016) 1:500 and DAPI (Invitrogen).
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bioRxiv - Cell Biology 2020Quote: ... and adapted to 2i condition by growing them in gelatin-coated flasks in N2B27 medium (50% neurobasal medium [Gibco], 50% DMEM/F12 [Gibco], 2 mM L-glutamine [Gibco] ...
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bioRxiv - Cell Biology 2019Quote: ... and alexa 568-conjugated goat anti-rabbit IgG (H+L) antibody (no. A-11036, Thermo Fisher Scientific, 1:1,000 dilution).
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bioRxiv - Cell Biology 2019Quote: ... Alexa Fluor 594 labeled goat anti-mouse IgG IgG(H+L), F(ab’)2 Fragment (A-11020, (Molecular Probes, Inc.), Alexa Fluor 647 conjugate-Anti-Rabbit IgG(H+L) ...
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bioRxiv - Cell Biology 2020Quote: ... Libraries were then PCR amplified and indexed using Phusion Hot Start II High Fidelity Polymerase (Thermo Scientific, F 549-L). An equal molar pool of each library was sequenced on HiSeq2000 (Illumina ...
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bioRxiv - Cell Biology 2020Quote: ... Purified islets were then cultured for 24 hours in RPMI1640 (HEPES 25mM) + final 2 mM L-glutamine (Gibco 11875-093) + 10% FCS 95 + 1% Penicillin/Streptomycin ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were washed X3 with PBS and incubated in the dark for 60 minutes at RT with Alexa Fluor 594 goat anti-rabbit IgG (H+L) (1:500 dilution in blocking buffer) (Invitrogen). Coverslips were washed X3 with PBS ...
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bioRxiv - Developmental Biology 2021Quote: ... and 4.5 µl of RfxCas13d protein (3 mg/ml) were mixed in 1 µl of nuclease-free water with phenol red (Invitrogen). One to 5 nL of RNPs were injected into one- to two-cell stage Ancistrus sp ...
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bioRxiv - Developmental Biology 2021Quote: Mixed cerebellar cultures or glial fractions were prepared from P0-1 pups and cultured in serum-free “cerebellum” media (BME, Gibco; 2mM L-Glutamate, Gibco; 1% v/v BSA ...
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bioRxiv - Developmental Biology 2020Quote: ... cells were cultured in Neural Expansion Media (1.5 mg/mL Glucose, 73 μg/mL L-glutamine, 1x N2 media supplement [R & D systems] in Knockout DMEM/F12 [Invitrogen] ...
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bioRxiv - Developmental Biology 2020Quote: ... embryos were reverse crosslinked in 250 μL RIPA buffer with RNase inhibitors for 30 minutes at 70°C and RNA was extracted with TRIzol LS (Ambion) following manufacturer instructions.
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bioRxiv - Developmental Biology 2021Quote: ... 2 x 105 – 4 x 105 mESCs were cultured in 60mm Petri dishes in DFNB medium (Neurobasal medium/Gibco, DMEM F12 1:1/Gibco, 1.5 mM L-glutamine/Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... Stained platelets were then washed in blocking buffer and mounted on poly-L-ornithine-coated glass slides using ProLongTM Glass Antifade mountant (Invitrogen), covered with coverslips and stored at 4 °C until imaging by multiphoton microscopy.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... HL-60 cells were obtained from ATCC and maintained in RPMI 1640 medium containing L-Glutamine and 25 mM HEPES (Gibco) supplemented with 20% FBS (Wisent ...
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bioRxiv - Biophysics 2022Quote: ... was dialyzed overnight in 1 L of 1 × TB at 4 °C in a Slida-A-lyzer mini dialysis unit with 3,000 MWCO (Thermo Fisher) to reduce the amount of glycerol in the sample ...
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bioRxiv - Neuroscience 2021Quote: ... sections were then rinsed with PBS (3L L min) and incubated in secondary antibodies (1:500, Alexa 488 donkey anti-rabbit, Invitrogen, A21206 ...
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bioRxiv - Molecular Biology 2020Quote: ... Wild type and catalytic mutant H672N RNase L plasmids cDNAs (the backbone is pcDNA5-FRT-TO with CMV promoter, Invitrogen) used in this study were a generous gift from Dr ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 μl of the cDNA preparation was amplified in a 50 μl PCR reaction using 1.25 U Dream Taq (ThermoFisher Scientific) DNA polymerase in its supplied buffer with 0.2 mM dNTPs and 0.2 μM P1’ and gene-specific primers (MS2 ...
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bioRxiv - Molecular Biology 2021Quote: ... Flp-In T-REx 293 cells were co-transfected with a hNAA40 L(dTIS>CTC) or hNAA40S expression construct and the Flp recombinase encoding pOG44 plasmid (Invitrogen) in a 1:9 ratio (in 6-well plates at 60-70% confluency ...
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bioRxiv - Molecular Biology 2021Quote: ... TK6 and MCL-5 cells (Crespi et al. 1991) were cultured in suspension in 1x RPMI 1640 medium with L-glutamine (Invitrogen) supplemented with 10% horse serum (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... membranes were incubated in Odyssey Blocking Buffer containing HRP-conjugated anti-rabbit IgG (H+L) (1:2000, Life Technologies 31462) or anti-mouse IgG (H+L ...
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bioRxiv - Cell Biology 2022Quote: ... 3x105 cells were spun down at 200 g for 5 min and resuspended in 1 mL L-15 media (Gibco) containing 1μL SiR-tubulin (Cytoskeleton Inc. ...
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bioRxiv - Cell Biology 2022Quote: ... spontaneous Ca2+ sparks were recorded from quiescent cardiomyocytes loaded with fluo-4 AM (20 μmol/L, Molecular Probes, Oregon, USA) and superfused with Hepes-Tyrode solution containing (in mmol/L) ...
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bioRxiv - Cell Biology 2022Quote: ... the ON-TARGETplus human Myo10 siRNA-SMARTpool from Dharmacon RNA Technologies (L-007217-00) was transfected into WT Hela cells using Lipofectamine RNAiMAX reagent (Invitrogen). To increase knockdown efficiency ...
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bioRxiv - Cell Biology 2022Quote: U2OS cells at 60% confluency were transfected with CHC siRNA retrieved from Dharmacon (SMARTpool: ONTARGETplus; L-004001-01-0010) or control siRNA (Dharmacon; ON-TARGETplus CONTROL) using Lipofectamine 3000 (Invitrogen). At 24 h following transfection ...
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bioRxiv - Immunology 2022Quote: ... TRIM5 (L-007100-00-0005) or non-targeting control (D-001810-10-05) (Dharmacon) using Lipofectamine RNAiMAX Transfection Reagent (Invitrogen). Transfection medium was replaced after 18h with RPMI 1640 medium supplemented with 10% FCS and cells cultured for additional 3 days before infection.
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bioRxiv - Immunology 2022Quote: ... washed with complete DMEM (DMEM + 2 mM L-glutamine, 1 mM sodium pyruvate, 10 mM HEPES, 200 U/mL penicillin/streptomycin, 10% FBS; GIBCO) and resuspended in complete DMEM in 15 cm tissue culture plates overnight ...
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bioRxiv - Immunology 2022Quote: ... ileal tissue was collected and stored in stabilization buffer (2 mM ethylenediaminetetraacetic acid [EDTA; Invitrogen AM9260G], 2mM L-glutamine [Gibco 25-030] ...
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bioRxiv - Immunology 2021Quote: ... IRDye 800LT goat anti-rabbit (Li-Cor, 925-32210) or Alexa Fluor 680 donkey anti-sheep IgG (H+L; Invitrogen), and visualized using the Li-Cor Odyssey Imaging System ...
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bioRxiv - Immunology 2021Quote: ... mice were woken up to allow any fecal matter to evacuate and then anesthetized again to introduce 100μL of Cy-5-labelled glucose or Cy-5 secondary goat anti-rat antibody (0.1mM diluted in PBS, ThermoFisher A10525) into the colon via a gavage needle enema ...
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bioRxiv - Molecular Biology 2021Quote: ... falciparum Dd2 was cultured at 2-5% hematocrit in O+ erythrocytes in Malaria Culture Medium (MCM): RPMI 1640 supplemented with 5 g/L Albumax II (Gibco), 0.12 mM hypoxanthine (1.2 mL 0.1M hypoxanthine in 1 M NaOH) ...
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bioRxiv - Biochemistry 2019Quote: ... were grown to 80% confluence at 37°C with 5% CO2 on 78 cm2 dishes using DMEM high glucose (Dulbecco’s Modified Eagle, glucose 4.5 g/L, Gibco, 41966–029), 10% bovine serum (Gibco ...
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bioRxiv - Immunology 2019Quote: ... cells were centrifuged and re-suspended in DMEM supplemented with 2.5% (v/v) FBS plus 0.3 mg/ml L-glu or Gibco Hybridoma-SFM (12045076, Life Technologies, UK). 20 ml of 2×105 cells/ml were transferred to a 75 ml flask ...
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bioRxiv - Cell Biology 2019Quote: ... were cultured in DMEM media supplemented with 10% FBS (Gemini, prod. number 100–106) and 1 mM L-glutamine (ThermoFisher/Life Technologies ...