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Citations for Thermo Fisher :
7651 - 7700 of 10000+ citations for 6H 1 3 Dioxolo 4 5 g 1 benzopyran 6 one 7 8 dihydro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Purified PCR products and the vector were mixed at a 1:3 molar ratio and ligated using T4 DNA ligase (Thermo Scientific). For HiFi cloning ...
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bioRxiv - Microbiology 2023Quote: ... Lung organoids differentiation was performed in serum-free differentiation (SFD) medium of DMEM/F12 (3:1) (Thermo Fisher Scientific, Cat# 11320033) supplemented with N2 (FUJIFILM Wako Pure Chemical ...
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bioRxiv - Biochemistry 2022Quote: ... cells were checked for 60-70% confluency and infected with 1:3 dilution of the pseudoviral stock in Opti-MEM (ThermoFisher: 31985062). Cells were incubated in low serum condition for 24 hours at 37°C before both luciferase and flow cytometry assays.
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bioRxiv - Microbiology 2023Quote: ... Samples were washed with 0.1% Tween 20 in PBS 3 times and cover slips were affixed to slides using ProLong Gold Antifade Mountant (Thermo Scientific). Fibers were then imaged with a Leica Stellaris confocal microscope using a 63X oil immersion objective lens ...
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bioRxiv - Microbiology 2023Quote: ... and applied to plasma-cleaned CF-300 2/1 grids before being blotted for 3 seconds in a Vitrobot Mark IV (Thermo Fisher) and plunge frozen into liquid ethane ...
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Mucociliary Clearance Augmenting Drugs Block SARS-Cov-2 Replication in Human Airway Epithelial CellsbioRxiv - Microbiology 2023Quote: ... IDT cat. # 10006776) and Promega® GoTaq® 1 Step RT PCR (Cat. # A6020) in a QuantStudio 3 thermocycler (Applied Biosystems) and following the recommendations of the CDC (https://www.cdc.gov/coronavirus/2019-ncov/lab/virus-requests.html) ...
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bioRxiv - Neuroscience 2023Quote: ... sections were washed in TBST for 10 minutes 3 times and incubated in Alexa Fluor conjugated secondary antibodies diluted 1:200 (Life Technologies) with DAPI (1:50,000 ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated at room temperature for 3 h in TBS containing AlexaFluor-546-conjugated donkey anti-goat IgG secondary antibody (1:400, A-11056, Fisher Scientific), 0.5% Triton X-100 ...
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bioRxiv - Cell Biology 2023Quote: ... Beads were gently washed 3 times with 1 ml of KPBS using a DynaMag-2 magnet (Thermo Fisher Scientific cat# 12321D). Beads were resuspended in 1 ml of KPBS and transferred to a new tube ...
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bioRxiv - Molecular Biology 2023Quote: ... Coverslips were then washed 3 times with 1×PBS and stained with 0.5 µg/ml DAPI (Thermo Fisher Scientific, cat# D1306) in 1×PBS for 5 min ...
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bioRxiv - Molecular Biology 2023Quote: ... First-strand cDNA was synthesized in 20 μl reactions with 1-3 μg of RNA using 200 ng of random hexamer primers and 200 U Superscript III reverse transcriptase (Thermo Scientific), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... On day 5 the media was replaced with PC/SMC2 media for 24 hours and then cells were passaged 1:3 on to fibronectin (FC01010MG, Fisher Scientific) coated tissue culture plates in PC3 media ...
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bioRxiv - Microbiology 2023Quote: ... plates were washed 3 times with PBST and 50 μL of 1-Step Ultra TMB-ELISA was added (ThermoFisher Cat. #34028). Following a 2 to 5-min incubation ...
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bioRxiv - Neuroscience 2023Quote: ... The coverslips were washed 3 x in PBS and labeled with donkey anti-mouse AlexaFluor 647 (1:1000, A31571, Thermo Fisher), for 1 h at room temperature ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were then transfected with the gRNA library and NLS-Bxb1 recombinase plasmids at a ratio of 3:1 (w/w) using a FreeStyle Max transfection reagent (Thermo Fisher), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... Movies were recorded at a magnification yielding 0.84 Å/pixel with a dose rate of 16.3 e-/Å2/s and a defocus range between -1 to -3 μm using the Smart EPU Software (Thermo Fisher Scientific). Movies were built of 50 frames each with a total dose of 50.8 e−/Å2 (∼1 e−/Å2 per frame ...
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bioRxiv - Cell Biology 2023Quote: ... Secondary antibody incubation was for 3 h with Alexa Fluor 647 goat anti-mouse IgG (1:1000, Thermo Fisher Scientific, A21236) and Alexa Fluor 488 goat anti-rabbit IgG (1:1000 ...
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bioRxiv - Bioengineering 2023Quote: ... Sections were rinsed 3 x 1hr in 1XPBS and incubated with secondary antibody (AlexaFluor Plus 594 highly cross absorbed, 1:1000 (Molecular Probes)) for 1 hr at room temperature and then moved to 4°C for incubation overnight ...
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bioRxiv - Cell Biology 2023Quote: ... biopsies were co-cultured with 3T3-J2 fibroblasts and Rho-associated protein kinase inhibitor (Y-27632) in epithelial cell expansion medium consisting of a 3:1 ratio DMEM:F12 (Gibco, UK; 21765), 1X penicillin/streptomycin and 5% FBS (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... Slides were again washed 3 times with 50 mL of PBS and incubated with anti-mouse IgG-Alexa 488 secondary antibody (1:1000; ThermoFisher A28175) at 37°C for 1 hour ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... + hydrogen peroxidase 3% (1:100, Alexa Fluor 488 Tyramide SuperBoost Kit, goat anti-rabbit IgG, Invitrogen, Thermo Fisher Scientific, Ghent, Belgium) + Tris Buffer HCl pH 7,4 (1:1 ...
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bioRxiv - Developmental Biology 2023Quote: ... Samples were incubated in a solution of secondary antibodies diluted in blocking buffer (antibodies and dilutions described in Table 3) and DAPI (1 μg/mL; ThermoFisher Scientific) for 30 minutes at RT in the dark ...
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bioRxiv - Cell Biology 2023Quote: ... 24 hours after induction of GFP-KIF18A and endogenous KIF18A knockdown cells were fixed for 3 minutes in - 20oC methanol and subsequently stained for rabbit GFP 1:500 (Invitrogen, A11122) for one hour at room temperature with shaking.
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bioRxiv - Microbiology 2023Quote: ... On day 5 the media was replaced with PC/SMC2 media for 24 hours and then cells were passaged 1:3 on to fibronectin (FC01010MG, Fisher Scientific) coated tissue culture plates in SMC3 media ...
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bioRxiv - Plant Biology 2023Quote: ... Cleared tissue was washed in a PBS solution containing 0.1% SR2200 and then put into a PBS solution containing 0.1% SR2200 and a 1/1000 dilution of the nuclear stain TO-PRO-3 iodide (Thermo Fisher Scientific) for 30 minutes ...
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bioRxiv - Microbiology 2023Quote: ... The products were enzymatically cleaned (3 ul of PCR reaction, 2 ul water, 1 ul of ExoSap-IT (Applied Biosystems 78200)) for 15 minutes at 37°C followed by 15 minutes at 80°C ...
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bioRxiv - Immunology 2024Quote: ... at a 3:1 bead-to-cell ratio in T-cell media consisting of OpTmizer CTS SFM media (Thermo Fisher Scientific) supplemented with 5% human AB serum and 100 Units/mL human IL-2 (PeproTech) ...
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bioRxiv - Plant Biology 2024Quote: Total RNA was extracted from 3-week-old plants treated with 1 mM SA or H2O using Trizol39 (Thermo Fisher Scientific). DNase-treated total RNA was then used for SuperScriptIII Reverse Transcription (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed three times and incubated with Alexa546-anti- rabbit secondary antibody (1:1000 in 3% BSA/PBS, 200 µl/well, Invitrogen, A11035) for another hour at 37 °C ...
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The effect of single mutations in Zika virus envelope on escape from broadly neutralizing antibodiesbioRxiv - Microbiology 2023Quote: ... 1 µg of replicon was added to 3 µg of CprME plasmid and subsequently mixed with Lipofectamine 3000 (Invitrogen, Waltham, MA) and OptiMEM (Gibco BRI ...
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bioRxiv - Bioengineering 2023Quote: ... DNA was mixed with linear polyethyleneimine (PEI) at a ratio of 1 : 3 in Opti-MEM I-medium (Thermo Fisher Scientific). The transfection mixture was incubated for 20 minutes at room temperature and then added to the cell cultures ...
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bioRxiv - Neuroscience 2023Quote: ... Then cells were washed 3x for 5 min with PBS and incubated for 1 hour at RT with secondary antibodies (Supplementary Table 3) and Hoechst 33342 (Invitrogen, 62249). After 3 washes for 5 min with PBS ...
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bioRxiv - Immunology 2023Quote: ... at the end of cycle 1 (AC1) and at the end of cycle 3 (AC3) were thawed in AIM V medium (Thermo Fisher) with benzonase (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR for the 5’ and 3’ fragments was carried out using 0.2 µl (1 U) Phusion polymerase according to the manufacturer’s instructions (ThermoFisher Scientific, Vilnius, Lithuania). PCR products were purified with E.Z.N.A ...
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bioRxiv - Neuroscience 2023Quote: ... and then incubated with secondary antibody (AlexaFluor 546 donkey anti-mouse for PV, goat anti-rabbit for GluR2/3 or NPY, both 1:400, Thermo Fisher). Secondary antibody was dissolved in 1% normal donkey serum for PV or 1% normal goat serum for GluR2/3 or NPY ...
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Development of a genetically encoded sensor for probing endogenous nociceptin opioid peptide releasebioRxiv - Neuroscience 2024Quote: ... were transfected with wild-type human NOPR or NOPLight (0.8 μg DNA) and β-arrestin-2-mCherry (1 μg DNA) using 3 μL Lipofectamine 2000 (Thermo Fisher). After 24 h ...
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bioRxiv - Bioengineering 2024Quote: ... sections were similarly processed and incubated with mouse anti-Aggrecan (BC-3) primary antibody (1:50 in 2.5% NGS; Cat. No. MA3-16888; Thermo Fisher Scientific) and rabbit anti-Col-II primary antibody (1:100 in 2.5% NGS ...
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bioRxiv - Bioengineering 2024Quote: ... the slices were rinsed 3 times in PBS and incubated in the corresponding secondary antibody (1:500, Invitrogen, thermo Fisher Scientific) at 4°C overnight on a rotator ...
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bioRxiv - Bioengineering 2024Quote: ... the slices were rinsed 3 times in PBS and incubated in the corresponding secondary antibody (1:500, Invitrogen, thermo Fisher Scientific) at 4°C overnight on a rotator ...
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bioRxiv - Neuroscience 2024Quote: ... cochleae were plated in six-well plates (2-3 explants/well) containing 1 ml media (growth medium DMEM (12430-054,Gibco Life Technologies) combined with 1% FBS (16000-044 ...
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bioRxiv - Neuroscience 2024Quote: ... Retinas were washed in 1x PBS for 3×30 minutes at RT and transferred to secondary antibody solution containing 1:1000 goat anti-mouse Alexa 488 (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... 0.5 mM ATP and 1% fraction V BSA) and loaded with 3 μM Mag-Fura-2 AM (Thermo Fisher Scientific, #M1292) at room temperature for 30 min ...
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bioRxiv - Cell Biology 2022Quote: ... 1 μg of plasmid was used to transfect 750,000 cells in each well of a 6-well plate using Lipofectamine 2000 (5 μL; Thermo Fisher Scientific) in OptiMEM (400 μL ...
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bioRxiv - Molecular Biology 2020Quote: DR-U2OS and -HCT116 cells and EJ-U2OS and -HCT116 reporter cells were plated in 6 well plates and transfected with 5 μL Lipofectamine RNAiMAX transfection reagent (Thermo Fisher) and siRNAs (Invitrogen ...
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bioRxiv - Neuroscience 2019Quote: ... The medium was changed every day and the cells were passaged every 5-6 days using PBS without Calcium and Magnesium (Life Technologies).
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bioRxiv - Neuroscience 2020Quote: Spinal cord tissue from stage HH 25-26 (Day 5-6) chick embryos (Venkateshwara hatcheries Ltd.) were dissected out in L-15 medium (Gibco, HiMedia) with added 1x penstrep (Gibco) ...
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bioRxiv - Microbiology 2020Quote: ... HAP1 cells with a low passage number were seeded into 6 well microtiter plates at a density of 5 × 105 cells/well in IMDM medium (#21980032, ThermoFisher Scientific) containing 10 % FBS (Sigma Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... The medium was changed every day and cells were passaged every 5-6 days using PBS without Calcium and Magnesium (Life Technologies).
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bioRxiv - Biochemistry 2022Quote: ... 5 μl of each binding reaction was loaded directly onto a pre-run 6% acrylamide DNA retardation gel (Thermo Fisher Scientific) and electrophoresed at ≤20 V cm−1 at 4 °C ...
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bioRxiv - Genomics 2022Quote: ... 2.6 μL nuclease-free water and 5 μL cDNA template on the ABI Prism 7500 Fast Sequence Detection System (Life Technologies). Thermo-cycling conditions were as follows ...