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Citations for Thermo Fisher :
7801 - 7850 of 10000+ citations for rno mir 134 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... 1 uL RNaseOUT (40 U/uL) and 1 uL SuperScript IV RT (200 U/uL, Invitrogen) were added and the reaction mixture was incubated at 23 °C for 10 min ...
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bioRxiv - Bioengineering 2023Quote: ... 1 μL M-MLV Reverse Transcriptase (RT) (200 U/μL, Invitrogen, 28025-013, Breda, the Netherlands) and supplemented with RNAse-free ultra-pure water (ddH2O) ...
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bioRxiv - Cell Biology 2023Quote: ... for 10 minutes at room temperature (RT) in 96-well black MaxiSorp™ plates (475515, NUNC). Pooled normal human plasma (33% assay volume ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were incubated 30 min at RT with α-BTX-555 (10 μg/mL, Thermo Fisher) in PBT + 0.5% Triton X-100 + 10% NGS + 1% DMSO ...
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bioRxiv - Developmental Biology 2023Quote: ... Samples were assayed in triplicate for RT-qPCR using SYBR Green master mix (A25741, Applied Biosystems). Amplification was performed using the following settings ...
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bioRxiv - Cell Biology 2023Quote: ... organoids were for 30 minutes at RT blocked with 5% Normal Goat Serum (Thermo Fisher Scientific) in DPBS (Dulbecco’s PBS 0,01 M + 0,2% BSA) ...
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bioRxiv - Plant Biology 2023Quote: ... 500 ng of total RNA underwent reverse transcription using the RevertAid RT Reverse Transcription Kit (ThermoFisher) after treatment with DNase (ThermoFisher) ...
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bioRxiv - Plant Biology 2023Quote: ... RT-qPCR was performed with Fast SYBR Green master mix (Applied Biosystems Inc., Foster City, CA) in QuantStudio 3 Real-Time PCR System (ThermoFisher ...
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bioRxiv - Developmental Biology 2024Quote: ... and reverse-transcribed using the Superscript RT II Kit with random hexamers (Invitrogen, ThermoFisher Scientific, France). qPCR was performed using a SYBR Green master mix (EurobioGreen qPCR Mix ...
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bioRxiv - Developmental Biology 2024Quote: ... and reverse-transcribed using the Superscript RT II Kit with random hexamers (Invitrogen, ThermoFisher Scientific, France). qPCR was performed using a SYBR Green master mix (EurobioGreen qPCR Mix ...
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bioRxiv - Developmental Biology 2024Quote: Samples were equilibrated at RT for 15 minutes and fixed with 4% paraformaldehyde (Thermo Scientific, 043368.9M) for 15 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... After 4x10min washes in PBS-Tr at RT with 4′,6-diamidino-2-phenylindole (DAPI; Invitrogen) added during the third wash ...
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bioRxiv - Microbiology 2023Quote: ... Cell nuclei were stained at RT for 10 min with Hoechst DNA dye (1:100,000; Invitrogen). Coverslips were then mounted on glass slides with anti-fade reagent (Vector laboratories) ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was synthesized from isolated viral RNA by RevertAid RT Reverse Transcription Kit (Thermo Fisher Scientific). GCterm-Mpro-LNterm fragment sequence was amplified by PCR with primers (primer_for ...
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bioRxiv - Bioengineering 2023Quote: ... for 1 hour at RT with 1 μL/mL of DAPI (Thermo Fisher Scientific, Cat# D1306).
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bioRxiv - Cell Biology 2023Quote: ... Fixation was performed at RT for 10 min with 4 % formaldehyde (Thermo Fisher Scientific, cat.no. 28906), which was diluted in PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA purification was followed by cDNA synthesis with RevertAid RT Reverse Transcription Kit (Thermo Fisher Scientific). Sequencing was conducted via Nanopore or Sanger (MicroSynth AG ...
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bioRxiv - Systems Biology 2023Quote: ... This reaction involved the following components per sample: 44 μL of 5× RT Buffer (Thermo Scientific), 44 μL of 20% Ficoll PM 400 (Sigma Aldrich) ...
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bioRxiv - Genomics 2023Quote: ... and rt-qPCR (using PowerTrack™ SYBR Green Master Mix by Applied Biosystems Catalog no. A46012) was used to quantify gene expression in each pool ...
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bioRxiv - Bioengineering 2024Quote: ... Mature transcripts were reverse-transcribed using the SuperScript First- Strand Synthesis System for RT-qPCR (Invitrogen) with oligo (dT ...
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bioRxiv - Plant Biology 2024Quote: ... RT-qPCR analysis was performed using diluted cDNA as template for PowerUp SYBR Green (Applied biosystems) with the primers provided in SUPPLEMENTAL TABLE 1.
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bioRxiv - Developmental Biology 2024Quote: ... RT-qPCR was carried out using the TaqMan Fast Advance master Mix (ThermoFisher Scientific, Cat.#: 4444557) in a QuantStudio3 qPCR machine (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... before quantification of viral genome number via RT-qPCR (TaqPath COVID-19 CE-IVD Kit, ThermoFisher). Final supernatant was harvested and clarified by centrifugation at 4000 rpm for 10 minutes in a benchtop centrifuge then aliquoted and frozen at −80°C.
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bioRxiv - Genomics 2024Quote: ... which was then reverse-transcribed (RT) to create cDNA using Maxima reverse transcriptase (Thermo Fisher Scientific) and random hexamers ...
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bioRxiv - Neuroscience 2024Quote: ... RT-qPCR for oligodendrocyte lineage markers was performed using Platinum SYBR Green qPCR Supermix-UDG (Thermofisher). The sequence of the primers is listed in supplementary table S1.
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bioRxiv - Neuroscience 2024Quote: RT for miRNA analysis was performed using the TaqMan Advanced miRNA cDNA Synthesis Kit (Applied Biosystems). RT‒qPCR was performed using TaqMan Advanced miRNA Assays (Applied Biosystems ...
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bioRxiv - Molecular Biology 2024Quote: ... RT-qPCR was performed using the TaqMan™ Fast Advanced Master Mix (Applied Biosystems™ 4444556) and run on the Applied Biosystems™ QuantStudio™ 5 ...
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bioRxiv - Plant Biology 2020Quote: ... T-DNA left border (LB) end primers (Supplemental Table 5) and DreamTaq DNA polymerase (Thermo Fisher Scientific). The cycle settings used for the TAIL-PCR reactions were adjusted based on the characteristics of the polymerase and primers used and listed in Supplemental Table 6 ...
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bioRxiv - Cancer Biology 2021Quote: ... 20 pmol of each primer pair and nuclease-free water on a StepOne Plus System (ThermoFisher scientific). The thermal profile consisted of 1 cycle at 95 °C for 10 min followed by 40 cycles at 95°C for 15 sec ...
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bioRxiv - Microbiology 2020Quote: ... pfpdi8 cDNA was prepared using TRIzol-extracted mRNA and reverse transcription with primer P20 (SuperScript III, Invitrogen). All restriction enzymes used in plasmid construction were purchased from New England Biolabs.
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bioRxiv - Developmental Biology 2021Quote: ... 15 µl of 100 µM forward primer and 24 µl of 10mM each dNTPs (Thermo Fisher Scientific), 30 µl of 5X buffer and 57.5 µl of water were added to 20 µl of RNA product then incubated in 65 °C for 5min for denaturing ...
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bioRxiv - Cell Biology 2020Quote: ... SeV specific primers were used described in CytoTune®-iPS 2.0 Sendai Reprogramming Kit protocol (Thermo Fisher).
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bioRxiv - Developmental Biology 2020Quote: ... The reverse transcription reaction was performed with random hexamer primers and RevertAid Reverse Transcritase (Thermo Fisher Scientific) immediately after inactivation of DNase I with 5 mM EDTA at 65 °C ...
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bioRxiv - Pathology 2022Quote: ... as reported previously.8 Total RNA was reverse transcribed into cDNA using random primers (Thermo Fisher Scientific). Real-time PCR reactions were performed using a QuantStudioTM 12K Flex Real-Time PCR System with TaqMan Universal PCR Master Mix and TaqMan Gene Expression Assays (probes ...
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bioRxiv - Cell Biology 2022Quote: ... random primers and following the protocol of the high-capacity cDNA reverse-transcription kit (Thermo Fisher Scientific). mRNA expression levels of MIP1 ...
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bioRxiv - Molecular Biology 2020Quote: ... First strand cDNA was synthesized with a commercial kit using both oligo dT and random primers (Invitrogen). Second strand cDNA was synthesized with second strand synthesis buffer (Invitrogen) ...
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bioRxiv - Molecular Biology 2021Quote: ... An amount of 1 μg was reverse-transcribed using Superscript II with random hexamer primers (Thermo Fisher). Real-time PCR was performed using the KAPA probe fast universal master mix and the indicated primers on a Rotorgene 6000 machine ...
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bioRxiv - Genetics 2019Quote: ... RNA was transcribed to first-strand cDNA using oligo(dT)12-18 primer and Superscript II (Invitrogen). The resulting cDNA was used as template for qPCR (Bio-Rad ...
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bioRxiv - Biochemistry 2020Quote: ... and reverse transcribed using Oligo(dT)20 as a primer and Superscript III Reverse Transcriptase (Life Technologies) following the manufacturer’s protocol ...
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bioRxiv - Genetics 2020Quote: ... reference primers/probe (VIC, TaqMan™ Copy Number Reference Assay, human, RNase P, 4403326; ThermoFisher, Waltham, MA)] and applied to a X200™ Droplet Generator (1864002 ...
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bioRxiv - Molecular Biology 2019Quote: ... Reverse primer; CCCATGGTGACAGTCTTGAAGA. Promoter S. Region (−189 to −45. Probe; ACCTGAAGGACTCTTG with an MGB quencher (Taqman, Invitrogen), Forward primer ...
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bioRxiv - Developmental Biology 2019Quote: ... total RNA was reverse transcribed with random primers using the High Capacity cDNA Synthesis Kit (Applied biosystems). Gene expression was monitored by qPCR using genomic DNA to generate standard curves ...
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bioRxiv - Plant Biology 2019Quote: ... cDNA was synthesized from the isolated RNA using oligo dT primers and Superscript II reverse transcriptase (Invitrogen). qPCR was performed using the Bio-Rad CFX Connect Real-Time System (Bio-Rad) ...
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bioRxiv - Molecular Biology 2019Quote: ... Commercially available primer-probe mixes (Single Tube TaqMan Gene Expression Assays; Thermo Fisher Scientific, Waltham, MA, USA) were used for thioredoxin (Trx ...
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bioRxiv - Genetics 2021Quote: 3µg of RNA was reverse transcribed using SuperScript III kit and oligo(dT) primers (ThermoFisher Cat # 18080051) according the manufacturer’s protocol 100ng cDNA was amplified with AmpliTaq Gold™ 360 (Cat # 4398876) ...
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bioRxiv - Microbiology 2020Quote: ... using sequence specific primers (Extended Data Table 1) and Fast SYBR Green Master Mix (Applied Biosystems, 4385612). Flow cytometry was performed on BD FACS-Calibur (BD ...
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bioRxiv - Neuroscience 2021Quote: ... Retro-transcription was performed using oligo-dT primers and SuperScript® III First-Strand Synthesis (#18080093 Invitrogen). Gene specific primers were designed for amplification:
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bioRxiv - Molecular Biology 2021Quote: ... RNA was quantified and reverse transcribed using oligo(dT)20 primers and the SuperScript IV kit (ThermoFisher). RT-qPCR was performed on the LightCycler 480 II instrument (Roche) ...
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bioRxiv - Cancer Biology 2021Quote: ... along with target-specific primers in optical 96-well plates on ABI StepOne Plus Thermocycler (Applied Biosystems) with the following settings ...
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bioRxiv - Plant Biology 2020Quote: ... First strand cDNA was synthesised using polyA primers and RevertAid First Strand cDNA synthesis kit (ThermoFisher Scientific). For each RT-PCR reaction ...