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Citations for Thermo Fisher :
7751 - 7800 of 10000+ citations for rno mir 134 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... cDNA synthesis and RT-qPCR were carried out as described previously using first strand synthesis (Invitrogen) (47) ...
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bioRxiv - Immunology 2019Quote: ... RT-qPCR reaction mix was prepared according to manufactures protocol for TaqMan® (Applied Biosystems™). In 96-well plates ...
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bioRxiv - Molecular Biology 2019Quote: ... RT-qPCR for this assay was performed using PowerUp SYBR Green Master Mix (Thermo Fisher Scientific), specific primers for mature/spliced mRNAs (complementary to an exon-exon junction ...
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bioRxiv - Cell Biology 2020Quote: ... The Superscript II RT mix also contained: 0.5ul 200U/ul Superscript II reverse transcriptase (Life technologies), 2ul 5x Superscript II First-Strand Buffer (Life technologies) ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA was produced using 5 μL of each RNA sample and SuperScript™ IV-RT (Invitrogen) according to manufacturers’ instructions ...
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bioRxiv - Microbiology 2021Quote: ... Samples were thawed at RT and to 0·1 mg/ml RNAse A (EN0531, Thermo Scientific) was added and incubated for 15 min at 37 °C before the clean-up with the gDCC kit ...
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bioRxiv - Developmental Biology 2021Quote: ... then incubated for 8 minutes at RT with a solution of 3µM DHE (Thermo Fisher #D11347). Nuclei are stained with Hoechst ...
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bioRxiv - Cell Biology 2020Quote: ... 5% of the recovered RNA was reverse transcribed using the TaqMan miRNA RT kit (Life Technologies) and miR-145 ...
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bioRxiv - Cancer Biology 2021Quote: ... RT-qPCR was performed with Taqman Fast Advanced Master Mix (Thermo Fisher Scientific, catalog no. 4444557) on StepOnePlus™ Real-Time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... cells were fixed and permeabilized at RT using Fix/Perm kit (eBioscience, Affymetrix, San Diego, CA), and subsequently stained using the primary fluorophore-conjugated antibodies for 1 h on ice ...
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bioRxiv - Immunology 2020Quote: ... 3 μL RT reaction mix consisting of 3 μL of 50 μM random hexamers (Thermo Fisher), 0.8 μL of 25 mM deoxyribonucleotide triphosphates (dNTPs ...
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bioRxiv - Immunology 2021Quote: ... RT-qPCR was performed using Taqman Fast Virus 1-step Master Mix (Thermo Fisher Scientific, 4444434) in StepOne Plus real-time PCR thermocycler (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... RT reactions contained a total of 125μL with 12.5 μL of 10× SuperScript III buffer (Invitrogen), 12.5 μL of 50 mM MgCl2 ...
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bioRxiv - Developmental Biology 2020Quote: ... RT-qPCR was performed in triplicate using Fast SYBR Green Master Mix (ThermoFisher Scientific, Cat. #4385612) and StepOnePlus Real-Time PCR system (Applied Biosystems) ...
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bioRxiv - Cell Biology 2022Quote: ... 3.5 µL of RT master mix (1X first-strand buffer, DTT, RNaseOUT, SSIII [REF 56575, Invitrogen]) was added to each tube ...
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bioRxiv - Developmental Biology 2022Quote: ... RT qPCR was performed using Fast SYBR™ Green Master Mix (Applied Biosystems™, Thermo Fisher) and a CFX Connect™ Real-Time PCR Detection System ...
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bioRxiv - Developmental Biology 2022Quote: ... RT qPCR was performed using Fast SYBR™ Green Master Mix (Applied Biosystems™, Thermo Fisher) and a CFX Connect™ Real-Time PCR Detection System ...
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bioRxiv - Immunology 2022Quote: ... 2 hours at RT incubation with Alexa fluor 568-coupled Donkey anti-Rabbit IgG (ThermoFisher Scientific) was added to reveal anti-Mtb LAM staining ...
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bioRxiv - Molecular Biology 2022Quote: ... RT-qPCR was conducted on an Applied Biosystems QuantStudio 3 system (Thermo Fisher, Waltham, MA, USA) following the conditions ...
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bioRxiv - Bioengineering 2022Quote: ... Samples were processed via RT-qPCR using QSOX1 (Hs00170210_m1) and RPLP0 (Mm00725448) TaqMan probes (Thermo Fisher) and Bioline amplification mix ...
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bioRxiv - Cell Biology 2022Quote: ... SYPRO Orange protein gel staining 5000x and PrimeScriptTM RT reagent Kit were purchased from Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... for 1 h at RT and with 1:10 000 Goat Anti-Rabbit-HRP (#31460, Invitrogen), for 1 h at RT ...
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bioRxiv - Genomics 2022Quote: ... We prepared the Reverse Transcription mix by adding sequentially 50 μL of 5X RT Buffer (ThermoFisher), 7.8 μL of RNase-free water ...
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bioRxiv - Molecular Biology 2022Quote: ... RT-qPCR was conducted on an Applied Biosystems QuantStudio 3 system (Thermo Fisher, Waltham, MA, USA) following the conditions ...
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bioRxiv - Plant Biology 2022Quote: RT-qPCR experiments were performed with three biological replicates using a QuantStudio 7 cycler (Applied Biosystems). The 10 μl reaction mixture contained 5 μl 2X SYBR Green/ROX master mix (Applied Biosystems) ...
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bioRxiv - Plant Biology 2023Quote: ... cDNA was synthesised with the Maxima First Strand cDNA Synthesis Kit for RT-qPCR (ThermoFisher, K1642) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... cDNA (250 ng) was used for RT-qPCR in the Step One Plus system (Applied Biosystems), using miRCURY LNA SYBR Green PCR Master Mix and miRCURY LNA miRNA PCR primers (Qiagen) ...
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bioRxiv - Neuroscience 2022Quote: 500 ng of purified RNA (see above) was reverse transcribed (RevertAid RT Kit; ThermoFisher, Cat# K1691). The resulting cDNA was diluted 1:50 in water and subjected to RT-qPCR using the CFX384 Touch Real-Time PCR Detection System (BIO-RAD ...
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bioRxiv - Molecular Biology 2022Quote: ... Reverse transcription (RT) was performed using SuperScript IV First-Strand Synthesis System (Invitrogen, Grand Island, NY) with 1 μg of RNA in a final reaction volume of 20 μL after DNA was removed ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 500ng of RNA were subjected to reverse transcription using Maxima H Minus RT (Thermo Fisher Scientific) with oligo-dT primers ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... After centrifugation (400rpm, 5min, RT) hepatocytes were suspended in M199 + Glutamax (Invitrogen, Cat. no. 41150-020), 1% Penicillin/Streptomycin (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... One microgram of total RNA was retrotranscribed in cDNA using the RevertAID RT kit (ThermoFisher #K1622). qRT-PCR was performed using the SYBR Select Master Mix for CFX (Applied Biosystems ...
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bioRxiv - Bioengineering 2024Quote: ... Mature transcripts were reverse-transcribed using the SuperScript First- Strand Synthesis System for RT-qPCR (Invitrogen) with oligo (dT ...
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bioRxiv - Neuroscience 2023Quote: ... Cells cultured for RNA extraction were collected at RT in TRI Reagent™ Solution (Invitrogen; AM9738) by splashing the cells with TRI Reagent™ Solution using a p1000 ...
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bioRxiv - Molecular Biology 2024Quote: ... After 4x10min washes in PBS-Tr at RT with 4′,6-diamidino-2-phenylindole (DAPI; Invitrogen) added during the third wash ...
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bioRxiv - Developmental Biology 2024Quote: Samples were equilibrated at RT for 15 minutes and fixed with 4% paraformaldehyde (Thermo Scientific, 043368.9M) for 15 minutes ...
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bioRxiv - Developmental Biology 2024Quote: ... and reverse-transcribed using the Superscript RT II Kit with random hexamers (Invitrogen, ThermoFisher Scientific, France). qPCR was performed using a SYBR Green master mix (EurobioGreen qPCR Mix ...
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bioRxiv - Developmental Biology 2024Quote: ... and reverse-transcribed using the Superscript RT II Kit with random hexamers (Invitrogen, ThermoFisher Scientific, France). qPCR was performed using a SYBR Green master mix (EurobioGreen qPCR Mix ...
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bioRxiv - Molecular Biology 2024Quote: ... 500 ng of total RNA was reverse transcribed using the M-MLV RT kit (28025013; Invitrogen). TaqMan Fast Advanced Master Mix (4444557 ...
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bioRxiv - Molecular Biology 2024Quote: RT-qPCR reactions were prepared with the TaqMan™ Gene Expression Master Mix (Applied Biosystems # 4369016) and run and analysed on the StepOnePlus™ real-time PCR system (Applied Biosystems) ...
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bioRxiv - Bioengineering 2024Quote: ... One µg of total RNA was used to prepare cDNA using Super Script II RT (Invitrogen) and oligo dT primer (1 µL ...
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bioRxiv - Cancer Biology 2024Quote: ... RT-qPCR was performed using PowerUpTM SybrTM Green Master Mix (#A25741, Thermo Fisher Scientific, Waltham, MA) according to manufacturer’s protocol and utilized a BioRadTM CFX384 thermocycler ...
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bioRxiv - Genomics 2023Quote: ... and rt-qPCR (using PowerTrack™ SYBR Green Master Mix by Applied Biosystems Catalog no. A46012) was used to quantify gene expression in each pool ...
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bioRxiv - Immunology 2022Quote: ... RT-qPCR was conducted using LightCycler-480 SYBR Green I Power-Up Master Mix (Applied Biosystems) on a LightCycler-480 instrument (Roche) ...
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bioRxiv - Immunology 2023Quote: ... RT-qPCR was then performed on the cDNA samples using SYBR Select Master Mix (ThermoFisher Scientific) and the LightCycler 480 System (Roche) ...
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bioRxiv - Cell Biology 2023Quote: ... 2ug of total RNA was transcribed into cDNA using Superscript synthesis system for RT-qPCR (Invitrogen) following the manufacturer’s directions.
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bioRxiv - Microbiology 2023Quote: ... RT-qPCR was then performed using the Maxima SYBR Green/ROX qPCR Master Mix (Thermo Scientific) on a Rotor-Gene® Q MDx instrument (Qiagen) ...
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bioRxiv - Microbiology 2022Quote: ... Cycle threshold (Ct) values were determined during RT-qPCR analysis using StepOneTM system (Applied Biosystems, USA).
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bioRxiv - Microbiology 2022Quote: ... RT-qPCR analysis was performed using the Applied Biosystems AB7500 instrument (Applied Biosystems, Foster City, CA) and the RNA Ultrasense one-step RT-qPCR system (Invitrogen) ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 uL RNaseOUT (40 U/uL) and 1 uL SuperScript IV RT (200 U/uL, Invitrogen) were added and the reaction mixture was incubated at 23 °C for 10 min ...