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Citations for Thermo Fisher :
7651 - 7700 of 10000+ citations for rno mir 134 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... permeabilized and stained for intranuclear transcription factors using the eBioscience™ Foxp3/Transcription Factor Staining Buffer Set acording to manufacturer indications (ThermoFisher Scientific). The following antibodies were used for surface and intranuclear staining ...
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bioRxiv - Immunology 2022Quote: ... For staining of intracellular human Foxp3 and mouse T-bet cells were fixed and permeabilized by using an eBioscience Foxp3/Transcription Factor Staining Buffer Set (Thermo Fisher Scientific) and stained with Alexa Fluor 647-conjugated anti-human Foxp3 mAb (clone 259D ...
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bioRxiv - Immunology 2019Quote: ... SHIV RNA was visualized with the 1-Plex ViewRNATM ISH Tissue Assay Kit using SIVmac239 or Beta actin (positive control) probe sets and ViewRNA Chromogenic Signal Amplification Kit (ThermoFisher, Waltham, MA). These two sequential slides were indivudually imaged with a Zeiss AxioObserver microscope and AxioCam MRm camera ...
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bioRxiv - Molecular Biology 2019Quote: The Ankyrin and SET domains of EHMT1 were amplified from cDNA prepared using the Superscript III cDNA synthesis kit (11752-050, Thermo Fisher Scientific) from HDFs with the help of Ankyrin (737-1004 AA ...
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bioRxiv - Molecular Biology 2019Quote: ... The probe set was eluted in 25 µL UltraPure Water and the concentration was analyzed by NanoDrop on the ssDNA setting (Thermo Fisher Scientific). Probe sets with ssDNA concentration ranging from 200-500 ng/µL ...
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bioRxiv - Systems Biology 2020Quote: ... Cells were hybridized for 12 hours overnight with the following probe set specific conditions: 250 nM probe for Tnf/Il6/Csf2 in 2X SSC (Thermo Fisher Scientific) with 10% formamide (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... All reactions were set up in a 96-well plate at final volumes of 20 μl and SYPRO Orange (Thermo Fisher Scientific) was added as a probe at a dilution of 1:1000 ...
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bioRxiv - Systems Biology 2020Quote: ... cell density was set to 106 cell/ml in PBS and the resulting cell suspension was transferred onto adhesion slides (Thermo Fisher Scientific) at a density of 106 cells/side (0,33 x 106cells/spot) ...
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bioRxiv - Molecular Biology 2021Quote: ... labelled for an hour at room temperature using a TMT10plex Isobaric Label Reagent Set (0.8 mg per tag, 90110, ThermoFisher Scientific; LOT UK282322) and following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... The following media was supplied by a peristaltic pump set to deliver 8 ml/min volume: 150 μl wheat germ agglutinin conjugated to Alexa 594 dye (ThermoFisher Scientific, W11262) and 15 ml PBS 1x and 15 ml 4% PFA.
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bioRxiv - Systems Biology 2020Quote: ... The samples corresponding to either one TMT11plex or one TMTPro set were then mixed in a single glass HPLC insert (ThermoFisher C4010-630) and dried down to dryness in a speed-vacuum (Eppendorf ...
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bioRxiv - Bioengineering 2021Quote: ... The samples were allowed to culture for 12h at 37°C and cytokine concentration was measured in the media by Ready-Set-Go™ ELISA kits (Thermo Fisher) as detailed in the manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... according to the protocols set by the manufacturer before loading samples to the Applied Biosystems® 3500xL Genetic Analyzer (Applied Biosystems, USA) for sequencing using a fluorescence-based capillary electrophoresis analytical technique.
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bioRxiv - Neuroscience 2022Quote: ... Reverse transcription reactions were diluted 1:5 in water and qPCR reactions were set up with Luminaris HiGreen qPCR Master Mix (Thermo Fisher Scientific) on a LightCycler® 96 (Roche) ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA was extracted by TRIzol purification and DNase digestion (ThermoFisher TRIzol, 5PRIME PhaseLock Heavy, Invitrogen Purelink RNA columns, ThermoFisher Purelink DNase Set) and quantified with NanoDrop™ spectrophotometer.
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bioRxiv - Immunology 2022Quote: ... or corresponding isotype control antibodies diluted in FACS buffer for 30 min at 4 ° After fixation and permeabilization for 30 min at 4 °C using the Foxp3/Transcription Factor Staining Buffer Set (Thermo Fisher Scientific), resuspended cells were kept overnight at 4 °C ...
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bioRxiv - Pathology 2024Quote: APOE alleles were determined by genotyping of rs429358 and rs7412 as part of a larger sample set using either the TaqMan® SNP Genotyping Assay (ThermoFisher Scientific) for 13 of the AD cases ...
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bioRxiv - Synthetic Biology 2024Quote: ... All antibodies were diluted in blocking buffer as described in the Bioscience Foxp3/Transcription Factor Staining Buffer Set (ThermoFisher #00-5523-00).
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bioRxiv - Zoology 2024Quote: We obtained photographs of the coronal cross-sections set at the M1/M2 junction using Avizo v.7.0 (Thermo Fisher Scientific, Waltham). First ...
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bioRxiv - Microbiology 2024Quote: ... A mixed asexual culture of 1% was set up and cultured in medium supplemented with 2.5% Albumax II (Thermo Fisher Scientific™) without human serum for four days ...
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bioRxiv - Molecular Biology 2024Quote: 1.5 µg trypsinized peptides were dissolved in 50 mM TEAB pH 8.5 and labelled using the TMT10plex Isobaric Labeling Reagent Set (Thermo Fisher Scientific, 90111). Each sample was labeled with TMT10plex reagents at a 20:1 label:peptide w/w ratio ...
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bioRxiv - Cancer Biology 2024Quote: ... for the discovery set and the Recover AllTM Total Nucleic Acid Isolation Kit for the French external validation cohort (Invitrogen, Thermo Fisher Scientific).
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bioRxiv - Microbiology 2024Quote: ... Pellets were set to dry on the bench before being resuspended in 20 μl of The RNA Storage Solution (Ambion, Thermo Fisher Scientific) and stored at -80 °C.
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bioRxiv - Microbiology 2024Quote: ... using 3 to 5 day-old Anopheles coluzzii mosquitoes per glass mini feeder (Coelen Glastechniek, The Netherlands) that was attached to a circulating water bath set up at 38°C (Isotemp®, Fisher Scientific) 52,53 ...
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bioRxiv - Immunology 2023Quote: ... tumor necrosis factor alpha (TNFα) and C-C motif chemokine ligand 2 (CCL2) concentrations were evaluated using the Ready-SET-Go!™ ELISA kits (ThermoFisher Scientific). CCL24 concentrations were evaluated using the Mouse CCL24/Eotaxin-2/MPIF-2 DuoSet ELISA kit (R&D Systems) ...
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bioRxiv - Immunology 2022Quote: ... Stained cells were washed twice with PBS/BSA before preparation for intracellular staining using the Intracellular Fixation & Permeabilization Buffer Set (Thermo Fisher Scientific) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... cells were instead fixed and permeabilized with a 1X FoxP3 Fixation/Perm buffer (eBioscience FoxP3/Transcription Factor Staining Buffer Set, Thermo Fisher Scientific) for 30 min at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... cells were washed with FACS buffer and fixed Fix-Perm buffer of the eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set (Thermo Fisher) overnight ...
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bioRxiv - Molecular Biology 2023Quote: Singe particle and tomography data sets were acquired under cryogenic conditions on an FEI Titan Krios G3i TEM microscope (Thermo Fisher Scientific) operating at 300kV and equipped with a Bioquantum post-column energy filter and a Gatan K3 direct detector.
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bioRxiv - Cancer Biology 2023Quote: RNA dPCR reactions were set up using the following volumes: 2.4 µL 5X dPCR QuantStudio Absolute Q Master Mix (PN A52490, ThermoFisher Scientific, Waltham, MA), 2.4 µL 5X primer-probe mix (Table S3) ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: pCAF sorting based on α-SMA protein level: pCAFs were fixed and permeabilized by using the eBioscienceTM Intracellular Fixation & Permeabilization Buffer Set (Life Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... diluted in PBST for 5 min at room temperature and were then mounted using SlowFade Glass Soft-set antifade mountant (ThermoFisher Scientific, S36917) and 1.5H coverslips (VWR ...
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bioRxiv - Cancer Biology 2023Quote: ... A set volume of each (270 µL) was transferred to a 1.5 mL tube and dried down by speedvac (Thermo Fisher, Waltham, MA). Samples were reconstituted in 30 µL of Optima LC/MS grade water (Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... to ensure equivalent concentrations across each set of samples before being covalently labeled with TMTpro™ Isobaric Label Reagent 16-plex (Thermo Fisher Scientific Cat No ...
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bioRxiv - Microbiology 2023Quote: ... The final set of wells were gently washed with PBS with 100 μg/mL of gentamicin (Life Technologies Co., Grand Island, NY) for 2h ...
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bioRxiv - Immunology 2023Quote: ... Transcription factors intranuclear staining was performed using Foxp3/Transcription Factor Staining Buffer Set (Invitrogen by Thermo Fisher Scientific; ref # 00-5523-00) according to manufacturer instructions ...
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bioRxiv - Immunology 2023Quote: ... samples were fixed and permeabilized with a 1X FoxP3 Fixation/Permeabilization Buffer (eBioscience FoxP3/Transcription Factor Staining Buffer Set, Thermo Fisher Scientific) for 30min at 4°C.
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bioRxiv - Microbiology 2023Quote: ... 100 μg of protein from each condition was subjected to in-solution digestion before labelling the resultant peptides using the TMT-10plex Isobaric Label Reagent Set (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... labelled for an hour at room temperature using a TMTpro 16plex Isobaric Label Reagent Set (0.8 mg per tag, A44520, ThermoFisher Scientific; LOT VE299607) and following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... A separated set of LC sections were stained using the same protocols for TH and rabbit anti-DsRed (1:1000; Fisher Scientific NC9580775), along with the appropriate secondary goat anti-chicken 488 (1:500 ...
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bioRxiv - Microbiology 2024Quote: ... The digested samples were concentrated to near-dryness (1-5 μl) using a vacuum centrifuge then were labeled with TMT 10plex Isobaric Label Reagent Set (Thermo Fisher Scientific) as per the manufacturer’s protocol ...
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A conserved germline-specific Dsn1 alternative splice isoform supports oocyte and embryo developmentbioRxiv - Cell Biology 2024Quote: 1 µg trypsinized peptides were dissolved in 50 mM TEAB pH 8.5 and labelled using the TMT10plex Isobaric Labeling Reagent Set (Thermo Fisher Scientific, 90111). Each sample was labeled with TMT10plex reagents at a 20:1 label:peptide w/w ratio ...
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bioRxiv - Molecular Biology 2021Quote: ... was used or reverse transcription for mRNAs was performed using Superscript IV RT (Thermo Fisher Scientific) and oligo(dT ...
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bioRxiv - Developmental Biology 2021Quote: RNA was transcribed into cDNA according to manufacturer’s instructions (SuperScript III RT, Thermo Fisher Scientific, USA). RT-qPCR was performed in technical replicates using 1:1 cDNA in each reaction and a primer concentration of 0.5uM ...
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bioRxiv - Neuroscience 2021Quote: ... cDNA was generated from 1μg of total RNA using the RevertAid RT Reverse Transcription Kit (ThermoFisher) with a mixture of random hexamer and oligo dT primers ...
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bioRxiv - Molecular Biology 2020Quote: ... was used for one-step RT-qPCR and TaqMan Fast Advanced Master Mix (4444556, Life Technologies) was used for non-reverse transcriptase reactions ...
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bioRxiv - Neuroscience 2021Quote: ... Post-GEM-RT and post-cDNA amplification cleanup were performed using Dynabeads MyOne silane beads (Thermofisher) and SPRIselect (Beckman Coulter ...
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bioRxiv - Molecular Biology 2020Quote: ... along with 10% input RNA were then reverse transcribed by Superscript IV RT system (ThermoFisher, 18091050) using random hexamers ...
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bioRxiv - Immunology 2021Quote: ... RT-qPCR reactions consisted of 1 × PowerUP SYBR green mix (Thermo Fisher Scientific, Waltham, United States), 2.5 μL of diluted cDNA ...
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bioRxiv - Immunology 2021Quote: ... cDNA was synthesized from sorted cells using random hexamers and Superscript III RT (Thermo Fisher Scientific). Ig heavy chain genes were then PCR amplified separately with two rounds of nested PCR using DreamTaq Mastermix (Thermo Fisher Scientific) ...