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Citations for Thermo Fisher :
7701 - 7750 of 10000+ citations for rno mir 134 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2019Quote: ... GEM-RT were performed in a Veriti 96-well thermal cycler (Thermo Fisher Scientific, Waltham, MA), following which ...
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bioRxiv - Bioengineering 2019Quote: ... Cells were fixed for 60 min at RT with the Foxp3 Fixation/Permeabilization Buffer (Invitrogen 00552300), washed ...
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bioRxiv - Developmental Biology 2019Quote: ... cDNA was synthesized with the Maxima First Strand cDNA Synthesis Kit for RT-qPCR (Thermo Scientific). qRT-PCR was performed with a 1:2 dilution of cDNA by using the StepOnePlus Real-Time PCR-System (Thermo Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: RT-QPCR was performed on cDNA using the Taqman custom gene expression assay (Thermo Fisher Scientific) and the ABI Prism 7900HT sequence detection system (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2019Quote: ... 10 μl RNA was mixed with 4 μl 5 x RT buffer (Invitrogen, Breda, The Netherlands), 0.2 μl RNAsin (Promega ...
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bioRxiv - Systems Biology 2019Quote: ... Reverse transcription (RT) was performed using a high capacity RNA-to-cDNA kit (Thermo Fisher, USA). The specificity of PCR was verified by performing reactions without RT and melt-curve analysis ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... DEPC treated water and Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) kit (Applied Biosystems, 850 Lincoln Centre Drive ...
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bioRxiv - Systems Biology 2021Quote: ... followed by fluorescent labeling of incorporated EdU for 1 hour at RT (Thermo Fisher Scientific C10357). Finally ...
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bioRxiv - Biochemistry 2020Quote: ... Transcript levels were determined with RT-qPCR using StepOnePlus™ Real-Time OCR Systems (Applied Biosystems) on the cDNA matrix ...
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bioRxiv - Biochemistry 2021Quote: ... diluted in PBS for 20 min at room temperature (RT) (Thermo Fisher Scientific, Waltham, MA, USA). After fixation ...
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bioRxiv - Cell Biology 2021Quote: ... for 10 min at RT and mounted in ProLong Gold Antifade Reagent (Thermo Fisher Scientific, USA). Antibodies used for immunocytochemistry are listed in supplementary table S1.
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bioRxiv - Biochemistry 2020Quote: ... Reverse transcription of purified RNA was obtained by RevertAid RT Reverse Transcription Kit (Thermo Fisher Scientific). The quantification of gene transcripts was performed by quantitative PCR (Q-PCR ...
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bioRxiv - Plant Biology 2021Quote: ... Equal amounts of RNA (1μg) from different samples were reverse transcribed using SuperScript II RT (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... RT in 1x PBS shacking and DAPI staining (1:5000 in PBS, Hoechst 33342, Life Technologies) was then performed for a maximum of 10min ...
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bioRxiv - Microbiology 2021Quote: ... RT-qPCR was performed using 5 ng of cDNA in SYBR green master mix (Applied Biosystems). RecA was used as a housekeeping gene of reference to calculate the threshold values (ΔΔCT ...
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bioRxiv - Neuroscience 2020Quote: ... and stored overnight at RT in 5x SSC buffer (Thermo Fisher Scientific, Fair Lawn, NJ, USA). The next day ...
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bioRxiv - Plant Biology 2021Quote: ... The cDNA was synthesized with Maxima First Strand cDNA Synthesis Kit for RT-qPCR (Thermo Scientific). The transcript levels were measured by quantitative real-time PCR (qPCR ...
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bioRxiv - Systems Biology 2020Quote: ... 1.65 μL of Nuclease-free water and 1 μL of Maxima H Minus RT (Thermo Scientific). Finally ...
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bioRxiv - Microbiology 2020Quote: ... and RT-QNAT was performed on a Veriti 96 Well Thermal Cycler (Applied Biosystems, Lincoln, USA) using an human interferon b1 assay (4331182 ...
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bioRxiv - Cell Biology 2021Quote: ... Then the primary antibody solution was added and incubated (GFAP, Thermo Fisher, 1:200 4h, RT), (TREM2 ...
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bioRxiv - Cell Biology 2022Quote: ... click reaction was performed during 30 minutes at RT with 1 µM Alexa488-azide (Invitrogen, A10266). Then ...
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bioRxiv - Cancer Biology 2022Quote: ... samples were incubated at RT with 15 μl of Streptavidin beads (Thermo Fisher Scientific, Dy-11205D) for 1 hour ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were then excised and mounted using Prolong Diamond antifade mountant overnight at RT (ThermoFisher Scientific). For co-staining of SARS-CoV-2 S and ciliated cells ...
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bioRxiv - Molecular Biology 2022Quote: ... The RT reaction was carried out with SuperScript II First-Strand Synthesis Kit (Invitrogen, 18080-051). qPCR was performed with gene specific primers that were listed in Supplementary Table 3 ...
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bioRxiv - Cancer Biology 2022Quote: ... and cDNA was synthesized using Superscript Reverse Transcriptase III (RT) according to manufacturer’s instructions (Invitrogen, #18080044). PCR was carried out with Fast SYBR Green master mix (#4385612 ...
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bioRxiv - Microbiology 2022Quote: ... Reverse transcription RT-qPCR data were collected and analyzed using the StepOnePlus (version 2.1; Applied Biosystems) software package ...
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bioRxiv - Plant Biology 2022Quote: ... The purified RNA thus obtained was reverse to cDNA using the RevertAid RT Kit (Thermo Scientific), according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Cycle threshold (Ct) values were determined during RT-qPCR analysis using StepOneTM system (Applied Biosystems, USA).
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bioRxiv - Microbiology 2020Quote: ... RT-qPCR was performed using Power Up™ SYBR™ Green Master mix kit (Applied Biosystems) and the previously described MLV and GAPDH primers (69) ...
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bioRxiv - Microbiology 2021Quote: ... Samples were amplified using a SuperScript III Platinum One-Step RT-qPCR kit (Invitrogen 11732-020) with a final reaction volume of 25 µl ...
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In vivo imaging of the kinetics of microglial self-renewal and maturation in the adult visual cortexbioRxiv - Neuroscience 2020Quote: ... Wako 019-19741) followed by secondary antibody solution (4 h, RT, AlexaFluor 488, 1:500, Invitrogen), mounted and coverslipped ...
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A rapid, highly sensitive and open-access SARS-CoV-2 detection assay for laboratory and home testingbioRxiv - Molecular Biology 2021Quote: ... 2 μl of the RT-LAMP product and 125 nM ssDNA sensor (Invitrogen, DNaseAlert HEX fluorophor) were added to 20 μl of the Cas12-RNP complex on ice ...
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bioRxiv - Cancer Biology 2019Quote: ... The RNA samples were subjected to RT using SuperScript IV VILO following the manufacturer’s instructions (Invitrogen). Real-time quantitative RT-PCR (qRT-PCR ...
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bioRxiv - Physiology 2020Quote: ... cDNA was synthesized from 500 ng RNA using SuperScript II RT (Thermo Fisher Scientific, Renfrew, UK), following manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... RT-qPCR detection of SARS-CoV-2 was performed on a QuantStudio 5 instrument (Applied Biosystems) using a TaqPath 1-step RT-qPCR Master Mix (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... 0.5 μl Revert Aid Premium RT (Maxima) and 8 μl ddH2O) according to the manufacturer (Thermofisher). Next ...
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bioRxiv - Bioengineering 2021Quote: ... for 10 min at RT and blocked with 10% (v/v) fetal bovine serum (FBS, Gibco) in TBST (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Bioengineering 2020Quote: ... for 30 minutes at RT followed by 30-minute blocking with 1% BSA/PBS (Thermofisher, 37525) at RT ...
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bioRxiv - Immunology 2022Quote: ... and reverse transcribed (RT) with a high-capacity cDNA Reverse Transcription kit (Applied Biosystems, Thermo Fisher). Commercially purchased human primary microglia cDNA (ScienCell ...
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bioRxiv - Immunology 2022Quote: ... and reverse transcribed (RT) with a high-capacity cDNA Reverse Transcription kit (Applied Biosystems, Thermo Fisher). Commercially purchased human primary microglia cDNA (ScienCell ...
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bioRxiv - Microbiology 2022Quote: ... reverse transcription (RT) was directly performed after thawing plates using SuperScript IV (Thermo Fisher, cat#18090200) as previously described60 ...
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bioRxiv - Developmental Biology 2022Quote: RT-qPCR was performed with PowerUp™ SYBR™ Green Master Mix (Applied Biosystems™ A25778) using Applied Biosystems QuantStudio 3 ...
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bioRxiv - Cell Biology 2019Quote: ... The surface was coated for 10 minutes at RT with carboxylated red fluorescent beads (100nm, Invitrogen) diluted at 2-3/1000 in deionized water ...
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bioRxiv - Plant Biology 2020Quote: ... Gene expression was scrutinized in reverse transcription-quantitative polymerase chain reaction (RT-qPCR) (QuantStudio3-Applied Biosystems). Twelve μl reaction mixture (Sybr Green—6 μl ...
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bioRxiv - Developmental Biology 2020Quote: ... RT-qPCR was performed in triplicate with an ABI PRISM 7500 sequence detection system (Applied Biosystems) to quantify the relative mRNA levels for various genes ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were incubated overnight at RT in a solution containing rabbit anti-GFP (1:2000, Invitrogen), guinea pig anti-VGLUT2 (1:5000 ...
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bioRxiv - Systems Biology 2020Quote: ... and 1ug of RNA was used to make cDNA using SuperScript IV RT Kit (Invitrogen 18090010). Quantitative PCR was done using KAPA SYBR® FAST Universal 2X qPCR Master Mix (KAPA Biosystems KK4601 ...
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Increased ACTL6A Occupancy Within mSWI/SNF Chromatin Remodelers Drives Human Squamous Cell CarcinomabioRxiv - Molecular Biology 2021Quote: ... cells were incubated for 1 hour at RT with secondary antibodies conjugated to Alexa-488 (Invitrogen). The slides were mounted with ProLong Diamond Antifade Mountant with DAPI (Invitrogen) ...
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bioRxiv - Immunology 2020Quote: ... cDNA was prepared with 1-2 ug of RNA using random hexamers and Revertaid RT (ThermoFisher). LT mRNA levels were quantified by Taqman qPCR with normalization to TATA-Box Binding Protein and compared to a standard curve (Maru et al. ...
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bioRxiv - Immunology 2020Quote: ... Suspensions were fixed for 10 min at RT using 1.6% PFA (Fisher Scientific, Hampton, New Hampshire) and frozen at −80°C.