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Citations for Thermo Fisher :
7601 - 7650 of 10000+ citations for rno mir 134 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... and 1X PCR buffer (ThermoFisher Scientific). Cycling conditions were as follows ...
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bioRxiv - Microbiology 2019Quote: ... and 1X PCR buffer (ThermoFisher Scientific). Cycling conditions were as follows ...
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bioRxiv - Microbiology 2019Quote: ... and 1X PCR buffer (ThermoFisher Scientific). Cycling conditions were as follows ...
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bioRxiv - Molecular Biology 2020Quote: ... purified by PCR Purification Kit (Invitrogen) and sent to Genewiz for sequencing.
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bioRxiv - Genomics 2019Quote: ... 10x PCR buffer (Invitrogen®, Germany), 200µM dNTPs ...
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bioRxiv - Genomics 2019Quote: ... 10x PCR buffer (Invitrogen®, Germany), 200µM dNTPs ...
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bioRxiv - Genomics 2021Quote: ... 1.0 μl PCR mix (Affymetrix 77330), 2.0 mM dUTP (Affymetrix 77206) ...
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bioRxiv - Bioengineering 2019Quote: ... A PCR kit (ThermoFisher Scientific #F553S) with a 57°C annealing temperature was used for all PCRs following standard protocols.
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bioRxiv - Microbiology 2019Quote: ... PCR purification combo Kit (Invitrogen, USA) and EZ-10 Spin Column PCR purification Kit (BioBasic ...
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bioRxiv - Plant Biology 2021Quote: ... PCR was done using Pfu (Invitrogen) polymerase under the following conditions ...
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bioRxiv - Cell Biology 2020Quote: ... 10X PCR buffer II (Applied Biosystems), and 1.0 mM or 1.5 mM of MgCl2 (Applied Biosystems) ...
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bioRxiv - Immunology 2021Quote: ... Universal PCR Master Mix (Applied Biosystems) and the QuantStudio 5 Real-Time PCR system (Applied Biosystems by Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... PCR machine (Thermo Fisher Scientific, USA), chemidoc (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2020Quote: ... Platinum PCR Supermix (Thermo Fisher Scientific) and/or Q5® High-Fidelity DNA Polymerase (New England BioLabs ...
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bioRxiv - Molecular Biology 2022Quote: ... and Taq PCR reagent kit (Invitrogen) to amplify the ITS1-5.8S-ITS2 segments using the following touch-down protocol ...
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bioRxiv - Systems Biology 2022Quote: ... 50ul Phusion PCR master mix (ThermoFisher), 5ul of 10uM forward and 5ul of 10uM reverse primers with illumina adaptors and indexes were added to a total volume of 100ul ...
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bioRxiv - Neuroscience 2024Quote: Green PCR Master Mix (Thermo Fisher) in 384-well format using a 7900HT Fast Real-Time PCR System (Applied Biosystems) ...
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Chimeric Antigen Cytotoxic Receptors for In-Vivo Engineering of Tumor-targeting Natural Killer CellsbioRxiv - Immunology 2023Quote: ... GeneJet PCR purification kit (ThermoFisher Scientific) was used to linearize and purify the NK-CAR plasmids according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2023Quote: DreamTaq Green PCR Master Mix (ThermoFisher) was used with 10 ng/μl diluted genomic DNA ...
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bioRxiv - Biochemistry 2023Quote: QuantStudio 6 PCR system (Life Technologies). Data were normalized with the maximal fluorescence intensity as 100%.
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bioRxiv - Microbiology 2023Quote: ... Green PCR Master Mix (Applied Biosystems), 250 nM of forward and reverse primers and 1 μL cDNA ...
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bioRxiv - Microbiology 2023Quote: ... Real-time PCR instrument (Applied Biosystems). Relative fold changes of mRNA levels determined by RNA-seq were normalized to the expression of the ribosomal protein gene bcrsm18 ...
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bioRxiv - Cancer Biology 2023Quote: ... universal PCR Master Mix (Applied Biosystems) and the QuantStudio 7 System (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR employed Platinum Taq (Thermo Scientific) together with standard COI barcoding primers (C_LepFolF/C_LepFolR ...
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bioRxiv - Microbiology 2023Quote: ... SYBR green PCR Mastermix (Applied Biosystems) was used in a 20 µl volume ...
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bioRxiv - Cancer Biology 2023Quote: ... The PCR reaction (Thermo Scientific, EP0703) for Trim28 and Tp53 alleles comprised 1 µl diluted biopsy lysate and 19 µl reaction master mix (1X DreamTaq Buffer ...
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bioRxiv - Immunology 2024Quote: ... Phusion PCR (ThermoFisher Scientific, Waltham, Ma) was performed to amplify the 5’ junctions using the following primers 5’f1 ...
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bioRxiv - Microbiology 2023Quote: ... DreamTaq Green PCR Master Mix (ThermoFisher) was added to each tube with 0.2 μM ITS4 and ITS5 primers to amplify the internal transcribed sequences ...
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bioRxiv - Cell Biology 2024Quote: ... by Phusion PCR (Thermo Fisher Scientific) using primers flanked by ClaI and XbaI restriction enzymes (New England Biolabs) ...
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bioRxiv - Immunology 2024Quote: ... One-step qRT PCR (Applied Biosystems) was carried out according to the manufacturer’s recommendations ...
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bioRxiv - Genetics 2024Quote: ... and a PCR cycler (Applied Biosystems). The zebrafish gene beta actin ...
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bioRxiv - Cancer Biology 2024Quote: ... and StepOnePlus PCR system (Applied Biosystems). All the qRT-PCR probes (mouse IL-6 ...
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bioRxiv - Microbiology 2024Quote: TaqMan™ Universal PCR Mix (ThermoFisher) was used for Quantitative PCR (qPCR) ...
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bioRxiv - Cell Biology 2020Quote: ... Overview tile sets for the grids were acquired at 256 x magnification with the scanning electron beam using MAPS software (Thermo Fisher Scientific). The grids were then sputter coated with platinum for 15 seconds to minimise charging effect with electron beam ...
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bioRxiv - Immunology 2022Quote: ... and then stained with anti–Foxp3 at 23°C for 30 min using a Foxp3/Transcription transcription factor buffer set (Invitrogen, ThermoFisher Scientific). Samples were acquired with BD FACSCelesta (BD Biosciences) ...
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bioRxiv - Developmental Biology 2019Quote: Samples were divided into two groups— peripheral blood- and cord blood-derived cells— such that each set could be separately labeled with TMT 6-plex isobaric mass tagging reagents (Thermo Fisher Scientific) as previously described (41) ...
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bioRxiv - Plant Biology 2019Quote: ... Data normalization applied to each experiment set of microarrays was dependent on platform technology (RMA for Affymetrix and Quantile Normalization for Agilent).
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bioRxiv - Molecular Biology 2019Quote: ... The flow rate was set to 300 nL/min for separation on the analytical column (Acclaim pepmap RSLC 75 μM × 15 cm nanoviper; Thermo Fisher Scientific). Mobile phase A was composed of 99.9% H2O (EMD Omni Solvent ...
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bioRxiv - Immunology 2021Quote: ... fixed and permeabilized cells following manufacturer’s instructions for the eBioscience Foxp3/Transcription Factor Staining Buffer Set (Thermo Fisher Scientific, Waltham, MA, USA), barcoded samples using mass-tag cellular barcoding reagents diluted in Maxpar Barcode Perm Buffer (Fluidigm ...
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bioRxiv - Molecular Biology 2021Quote: ... HIV unspliced mRNA was thenlabelled with a set of 40 probe pairs against the GagPol region of the vRNA (catalogue number GagPol HIV-1 VF10-10884, Thermo Fisher Scientific) diluted 1:5 in diluent provided in the kit and hybridized to the target mRNA for 2 hours at 40°C ...
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bioRxiv - Physiology 2020Quote: ... A transient knockdown experiment was performed by transfecting siRNAs against Angptl4 and Ampk (sets of 4 SMART POOL siRNAs, Dharmacon, USA) in HepG2 cells using RNAiMAX (Life Technologies, USA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: One set of sections spaced 0.1 mm apart and spanning the entire block was stained with FluoroMyelin™ Green (ThermoFisher Scientific, F34651) for 1 hour ...
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bioRxiv - Cell Biology 2021Quote: A549 cells were seeded in 6-well plates at 2 × 105 cells/well and transfected with a set of plasmids targeting a gene (Table S2) using Lipofectamine LTX (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... we integrated all the individual CEL files from selected data sets profiled on HG-U133 plus 2.0 (Affymetrix, Santa Clara, CA, USA), retrieved from GEO (GSE14333 ...
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bioRxiv - Bioengineering 2022Quote: ... CCL5 or IL-2 was performed according to the protocol of eBioscience™ Intracellular Fixation & Permeabilization Buffer Set (Cat.88-8824-00, Thermo Fisher). Briefly ...
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bioRxiv - Bioengineering 2022Quote: ... The analytical column temperature was set at 40°C and was directly connected to a nanospray electron ionization source (Thermo Fisher Scientific), which generated a stable spray at a voltage of 1.8 kV ...
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bioRxiv - Neuroscience 2022Quote: ... 50-75 μg of soluble protein per sample (same for all samples in a set) was run on 10% Bis-Tris Bolt PAGE gels (Fisher Scientific, #NW00100BOX), and wet-transferred to nitrocellulose membrane (Protran 0.2 μm NC ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were washed with FACS buffer once and stained with the second set of following antibodies: Pacific blue-conjugated streptavidin (1:100, Thermo Fisher Scientific), AF488-conjugated anti-CD150 (TC15-12F12.2 ...
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bioRxiv - Cell Biology 2021Quote: Downregulation of the 40 candidate proteins (Figure 1B and Figure 1 – figure supplement 1A) was conducted by using two different sets of pre-designed ON-TARGETplus SMARTpool custom siRNA libraries (Thermo Fisher Scientific). p97 was used as both positive and loading control ...
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bioRxiv - Microbiology 2021Quote: ... mRNA was labelled with a set of 40 probe pairs against the GagPol region of the vRNA (catalogue number GagPol HIV-1 VF10-10884, Thermo Fisher Scientific) diluted 1:5 in diluent provided in the kit and hybridized to the target mRNA for 2 hours at 40°C ...