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Citations for Thermo Fisher :
7551 - 7600 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... using the dsDNA HS assay kit (Thermo Fisher Scientific, USA). Nanopore sequencing library was prepared with the Rapid Barcoding sequencing kit (SQK-RBK004 ...
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bioRxiv - Molecular Biology 2024Quote: ... coli TOP10 (Invitrogen) chemically competent cells ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA concentrations were quantified by Qubit 4 Fluorometer (QubitTM4 Fluorometer, Thermo Fisher Scientific, USA) using the dsDNA HS assay kit (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... The amplified operon was TOPO-ligated to pEXP5-CT vector (Invitrogen), and transformed to E ...
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bioRxiv - Molecular Biology 2024Quote: Microscope samples were prepared by sandwiching agarose gel pads (rich, defined media with 2 % Invitrogen UltraPure agarose) of approximately 100 µL between microscope slides and acid-cleaned #1.5H coverslips (Marienfeld) ...
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bioRxiv - Neuroscience 2024Quote: ... The slice was transferred onto a piece of parafilm and covered with a 30 μl droplet of 100 nM NeutrAvidin Protein (Thermo Fisher, #31000) in 1% BSA biotin free / Tyrode’s or 30 μl of 1% BSA biotin free / Tyrode’s alone (control ...
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bioRxiv - Neuroscience 2024Quote: ... and 15µg/mL blasticidin (ThermoFisher Scientific). To obtain a cell line with stably-integrated PAClight1P78A or PAClight1 expression cassettes ...
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bioRxiv - Neuroscience 2024Quote: ... L.V.s were concentrated by ultracentrifugation and resuspended in phosphate-buffered saline (dPBS, Gibco, Life Technologies, Zug, Switzerland) supplemented with 1% bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2024Quote: ... hSlack-WT cells were cultured in media supplemented with 500 μg/mL G418 (Thermo Scientific). Cells were split every 3-4 days when confluent using TrypLE Express (Gibco) ...
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bioRxiv - Neuroscience 2024Quote: ... in a Sylgard 170-lined dish (Thermo Fisher Scientific). The STNS was then dissected free from muscles and connective tissue and pinned in a Sylgard 184-lined petri dish (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... cells were washed in 1x PBS before detachment with TrypLE Express (ThermoFisher) and subsequent centrifugation at 150xG for 3 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... The STNS was then dissected free from muscles and connective tissue and pinned in a Sylgard 184-lined petri dish (Thermo Fisher Scientific) (Fahoum and Blitz ...
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bioRxiv - Neuroscience 2024Quote: Human Embryonic Kidney 293T (HEK) cells (ATCC) were maintained in high glucose DMEM media (Gibco). Media was supplemented with 10% FBS (Millipore Sigma ...
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bioRxiv - Neuroscience 2024Quote: ... was prepared by dissolving it in optima water (Thermo Fisher Scientific) at 10 mM ...
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bioRxiv - Neuroscience 2024Quote: ... CYFIP1 knockdown was performed using Silencer Select siRNA against the cyfip1 gene (Thermo Scientific: s23242)25 ...
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bioRxiv - Neuroscience 2024Quote: HEK293 cells were seeded in 6-well plates and transfected with 0.25μg LgBiT-miniG (miniGs or miniGsq45) or LgBiT-β-arrestin240 and 0.25μg SmBiT-tagged receptor plasmids using 3µL Lipofectamine 2000 (Thermo Fisher). 24 hours after transfection ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed in PBS and then incubated for 1 h in 1:500 diluted goat anti-chicken IgG (H+L) cross-adsorbed secondary antibody (Alexa fluor 488; Fisher Scientific A11039). The mounted slices were imaged using a Leica SP8 X confocal microscope equipped with a 63x 1.3 NA glycerol immersion objective.
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bioRxiv - Neuroscience 2024Quote: ... neurons were impaled with a microelectrode that was tip-filled with AlexaFluor-568 hydrazide (10 mM in 200 mM KCl; Thermo Fisher Scientific) and backfilled with squid internal solution ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were split every 3-4 days when confluent using TrypLE Express (Gibco). For all immunoblotting and imaging experiments ...
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bioRxiv - Neuroscience 2024Quote: ... in 500 μL optiMEM (Gibco) media with 5 μL lipofectamine.
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bioRxiv - Neuroscience 2024Quote: Chinese Hamster Ovary (CHO) cells were maintained similar to HEK cells with the following differences: Culture media was IMDM (Invitrogen) supplemented with 10% FBS ...
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bioRxiv - Neuroscience 2024Quote: ... Silencer Select Negative Control 1 (Thermo Scientific) was used as a control ...
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bioRxiv - Neuroscience 2024Quote: FMRP knockdown was performed using a silencing RNA (siRNA) cocktail made with equal parts of three Silencer Select siRNA against the fmr1 gene (Thermo Scientific: s5315, s5316, s5317)25 ...
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bioRxiv - Neuroscience 2024Quote: Protein lysates from HEK cells or two-month-old mice frontal cortex were prepared using Pierce IP Lysis Buffer (Thermo Scientific) supplemented with cOmplete EDTA-free Protease Inhibitor Cocktail (Millipore Sigma ...
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bioRxiv - Neuroscience 2024Quote: ... Transient transfection was performed 24 hours after plating using Lipofectamine 2000 (Thermo Scientific) according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... ∼1 million cells were dissociated with addition of TrypLE Express (ThermoFisher) and thoroughly washed with PBS ...
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bioRxiv - Neuroscience 2024Quote: ... and 1% HT supplement (Invitrogen). Cells were split approximately every 2 days ...
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bioRxiv - Neuroscience 2024Quote: ... anti-mouse-HRP 1:1000 (Thermo Scientific: 32430), anti-goat 1:10,000 (Thermo Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... EdU detection was performed by using the Click-iT® reaction cocktail (Click-iT® EdU, Invitrogen). Afterwards ...
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bioRxiv - Neuroscience 2024Quote: ... antibiotic-antimycotic (1X; Gibco), BIT 9500 serum substitute (10% ...
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bioRxiv - Neuroscience 2024Quote: ... Quality and quantity were assessed using an Agilent DNA1000 series chip assay and Invitrogen Qubit HS Kit (Invitrogen), respectively ...
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bioRxiv - Neuroscience 2024Quote: ... anti-p-FMRP 1:1000 (Thermo Scientific: PA5-35389), anti-CYFIP1 1:500 (Santa Cruz ...
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bioRxiv - Molecular Biology 2024Quote: Proteins were extracted from in ice-cold RIPA lysis buffer (ThermoFisher Scientific) or nuclear and cytoplasmic extraction reagents (for protein isolation from nuclear and cytosolic fractions ...
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bioRxiv - Molecular Biology 2024Quote: Cells were grown to confluence in growth medium and treated with CellROX® Green reagent at a final concentration of 5μM (Invitrogen, C10444) for 30Lmin at 37L°C to detect intracellular ROS by a Leica TCS-SP8 fluorescence microscope ...
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bioRxiv - Molecular Biology 2024Quote: ... and VE Cadherin (1:100; PA5-19612) were obtained from Invitrogen; antibody against AQP1 (1:750 and 1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... anti-goat 1:10,000 (Thermo Scientific: 31402), anti-chicken-HRP 1:20,000 (Jackson Laboratories ...
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bioRxiv - Neuroscience 2024Quote: ... 20U/mL SUPERase-In RNase Inhibitor (Thermo Scientific). 2mL of sucrose cushion was added to 13x51mm Ultra Clear tubes (Beckman) ...
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bioRxiv - Neuroscience 2024Quote: ... they were passaged using 0.25% trypsin-EDTA (Gibco) to a concentration of approximately 1-1.5 million cells per 10cm dish ...
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bioRxiv - Neuroscience 2024Quote: Mitochondrial membrane potential was quantified with JC-1 dye (Invitrogen, Waltham, MA). Neurons were treated for 2 hours and then incubated with 1ug/mL JC-1 dye for 30 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... using PowerUp SYBR Green Master Mix (Thermo Scientific) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Protein quantification was performed using Pierce BCA Protein Assay Kit (Thermo Scientific). For standard immunoblotting experiments or coimmunoprecipitation input controls ...
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bioRxiv - Neuroscience 2024Quote: ... Lipid droplets were detected using HCS LipidTox Red following manufacturer’s protocol (H34476; Thermo Scientific).
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bioRxiv - Neuroscience 2024Quote: ... Media was changed to 2 mL phenol red-free DMEM media (Gibco) supplemented with 25 mM HEPES and 4 mM L-glutamine with any denoted drugs 30 minutes (except where noted ...
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bioRxiv - Neuroscience 2024Quote: ... blots were stripped as necessary with 5mL Restore Plus Western Blot Stripping Buffer (Thermo Scientific) followed by three 5-minute washes ...
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bioRxiv - Neuroscience 2024Quote: ... GlutaMAX (1X; Invitrogen), and heparin (1µg/mL) ...
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bioRxiv - Neuroscience 2024Quote: ... and secondary antibody Alexa-Fluor 488 anti-chicken (1:500, Thermo Scientific: A-11039) as described previously70 ...
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bioRxiv - Neuroscience 2024Quote: ... The base media for culturing proliferating phNPCs included the following components: neurobasal A medium (Invitrogen), antibiotic-antimycotic (1X ...
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bioRxiv - Neuroscience 2024Quote: ... anti-rabbit-HRP 1:1000 (Thermo Scientific: 32460), anti-mouse-HRP 1:1000 (Thermo Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... The purified library was transformed into MegaX D10 electrocompetent cells (Invitrogen; Waltham, MA) which were cultured in 4L of LB broth with 1X ampicillin until the culture reached an optical density of about 1.0 ...
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bioRxiv - Neuroscience 2024Quote: ... AT-8 (MN1020; Thermo Scientific), MC-1 (Peter Davies lab) ...