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Citations for Thermo Fisher :
7601 - 7650 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... 1% penicillin/streptomycin (Gibco, ThermoFisher Scientific, Grand Island, NY, USA), and 2mM L-glutamine (Gibco ...
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bioRxiv - Cell Biology 2024Quote: ... supplemented with 10% bovine calf serum (Gibco, ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... CD3 (14-0032-82, Thermo Fisher Scientific, 1:100), Ki67 (Servicebio ...
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bioRxiv - Cell Biology 2024Quote: ... All the cell lines were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Life Technologies) supplemented with 10% FBS 103 (Boehringer Mannheim ...
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bioRxiv - Cell Biology 2024Quote: ... supplemented with 10% bovine calf serum (Gibco, ThermoFisher Scientific, Grand Island, NY, USA), 1% penicillin/streptomycin (Gibco ...
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bioRxiv - Cell Biology 2024Quote: ... and mounded with Prolong gold anti-fade mound media (Invitrogen, #S36936) on glass slides ...
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bioRxiv - Cell Biology 2024Quote: ... Sk-MEL-30 cells were cultured in Dulbecco’s Modified Eagle Medium (Gibco, ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... and 2mM L-glutamine (Gibco, ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... RNA concentration and purity were determined using the NanoDrop2000c (Thermo Fisher Scientific). Reverse transcription of RNA into cDNA was accomplished with the PrimeScript RT reagent Kit with gDNA Eraser (RR047A ...
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bioRxiv - Cell Biology 2024Quote: ... The medium was replaced or cells were transfected with plasmids of GFP-Rab5Q79L 24 h after transfection using Lipofectamine LTX (Thermo Fisher Scientific, MA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... 1x GlutaMax (Gibco, #35050061) and 1x Pyruvate (Gibco ...
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bioRxiv - Cell Biology 2024Quote: ... incubated with 100 ng/ml EGF-Alexa 555 (Thermo Fisher Scientific, MA, USA) for 5 min ...
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bioRxiv - Cell Biology 2024Quote: A total of 5 × 104 MCF7 cells were seeded on coverslips and transfected with siRNAs (10 nM) using Lipofectamine RNAiMAX (Thermo Fisher Scientific, MA, USA) by reverse transfection according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... and 50 cm analytical column (Acclaim PepMap, 500 mm x 75 µm ID; C18; 2 µm; 100 Å, Thermo Fisher Scientific, Waltham, MA), running a 120 min reversed-phase buffer gradient at 225 nl/minute on a Proxeon EASY-nLC 1000 pump in-line with a Thermo Q-Exactive HF quadrupole-Orbitrap mass spectrometer ...
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bioRxiv - Cell Biology 2024Quote: All qPCR experiments were performed in Quant Studio 1 and SYBR Green from Thermo Scientific. Conventional PCR was performed using either cDNA or total DNA ...
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bioRxiv - Cell Biology 2024Quote: ... Secondary antibodies of goat anti-Rabbit IgG conjugated with Alexa Fluor 568 and anti-Mouse Alexa conjugated with Fluor 594 were purchased from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Cell Biology 2024Quote: Affinity-enriched proteomics samples were analyzed in positive mode using data-dependent acquisition (DDA) either by an Easy-nLC 1000 – Q Exactive Plus or an Easy-nLC 1200 – Orbitrap Eclipse tribrid system (Thermo Fisher). On-line chromatography was directly coupled to the mass spectrometric systems using a nanoelectrospray ionization source ...
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bioRxiv - Cell Biology 2024Quote: Cells were maintained in DMEM 4.5 g/L Glucose + GlutaMax (Gibco, #10566016) supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2024Quote: ... and 1x Pyruvate (Gibco, #11360070). SAMM50 Knock-Down (KD ...
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bioRxiv - Cell Biology 2024Quote: ... After sorting cells were resuspended in organoid medium (ENR) (DMEM/F-12, #11320033, Gibco; 1X PenStrep, #15140122, Gibco; 1X B27 supplement, #17504044, Gibco; 1X GlutaMAXTM supplement, #35050061, Gibco; 0,1% of 100 mM N-Acetyl-cysteine ...
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bioRxiv - Cell Biology 2024Quote: ... 10 nM dexamethasone (Invitrogen, USA) and 1% penicillin/streptomycin (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... anti-CD63 (Thermo Fisher Scientific, USA; diluted at 1:1000), anti- CD81 (GeneTex ...
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bioRxiv - Cell Biology 2024Quote: ... After sorting cells were resuspended in organoid medium (ENR) (DMEM/F-12, #11320033, Gibco; 1X PenStrep, #15140122, Gibco; 1X B27 supplement ...
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bioRxiv - Cell Biology 2024Quote: ... The combined cell mixtures were resuspended in minimal organoid medium (DMEM/F-12, Gibco, #11320074; 1X PenStrep, Gibco, #15140122 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were split every 7 days using 5-minute incubation with 0.5 mM EDTA-PBS (Thermo Fisher) at 37 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... Real time PCR was performed in triplicates using 1 µl cDNA and 9 µl master mix consisting of 5 µl SYBR Green PCR Mix (4309155, Applied Biosystems), 3 µl H2O and 1 µl of forward and reverse primers (0.5 µM final concentration) ...
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bioRxiv - Cancer Biology 2024Quote: ... quantified via the Pierce™ Quantitative Colorimetric Peptide Assay (23275, Thermo Scientific), and lyophilized using SpeedVac ...
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bioRxiv - Cancer Biology 2024Quote: ... These peptides were subsequently resuspended in 10 µl of solvent A (solvent A: 0.1 % formic acid in H2O) for analysis on an Orbitrap Fusion Lumos Tribrid mass spectrometer (Thermo Scientific, MA, USA) coupled to an EASY-nano-LC 1200 system (Thermo Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... N-glycosylation modification of proteins was assessed using the PNGase F Glycan Cleavage Kit (A39245, Gibco, USA). In brief ...
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bioRxiv - Cancer Biology 2024Quote: ... Resulting protein concentrations were determined with a Bicinchonic acid assay (BCA - Pierce BCA Protein Assay Kit, ThermoFisher, Germany) and 20ug of protein were filled up to 140ul with 100mM HEPES pH 8 and transferred to a 96 deep-well plate ...
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bioRxiv - Cancer Biology 2024Quote: Chromatin immunoprecipitation (ChIP) assay was conducted using the Pierce™ Magna ChIP assay kit (26157, ThermoFisher) as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... 1mM sodium pyruvate (Gibco) and 1000U/mL leukemia inhibitory factor (Millipore ...
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Macropinocytosis mediates resistance to loss of glutamine transport in triple-negative breast cancerbioRxiv - Cancer Biology 2024Quote: ... 0.2 × 106/well for other cells) were seeded on glass coverslips (Menzel Gläser, Thermo Scientific) in a 24 well plate and cultured for 72 h (dox-treated cells and corresponding controls ...
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bioRxiv - Cancer Biology 2024Quote: ... IFN-α/β Reporter HEK 293 Cells were obtained from InvivoGen and cultured in DMEM medium (Gibco-41965-039) complemented with 10% FBS ...
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bioRxiv - Cancer Biology 2024Quote: ... 2 mM L-glutamine (Gibco), 0.1mM β-mercaptoethanol (Sigma) ...
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bioRxiv - Cancer Biology 2024Quote: ... 1% penicillin-streptomycin (Gibco), 2 mM L-glutamine (Gibco) ...
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bioRxiv - Cancer Biology 2024Quote: ... washed again 3x with TBST buffer and then imaged using the SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific; cat. 34578) on a ChemiDoc imaging system from BioRad ...
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bioRxiv - Cancer Biology 2024Quote: ... protein was transferred to a nitro-cellulose membrane using the iBlot semi-dry system from Invitrogen, blocked in 5% milk in TBST ...
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Macropinocytosis mediates resistance to loss of glutamine transport in triple-negative breast cancerbioRxiv - Cancer Biology 2024Quote: ... Tandem mass spectrometry analysis was carried out on a Q Exactive-HFX mass spectrometer (ThermoFisher) using HCD fragmentation in positive mode ...
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bioRxiv - Cancer Biology 2024Quote: Transfection of mouse ES cells was performed with Lipofectamine 2000 (Invitrogen) in a 24-well plate as previously described (Xie et al ...
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bioRxiv - Cancer Biology 2024Quote: ... to ligate the fragments with a 1:3 insertion to vector ratio at 16C overnight and transformed all reactions into Stbl3 chemically competent cells (Thermo Fisher Scientific; cat. C737303). We performed bacterial clone screening and Sanger sequencing to verify that they were in frame and unmutated.
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Macropinocytosis mediates resistance to loss of glutamine transport in triple-negative breast cancerbioRxiv - Cancer Biology 2024Quote: ... Cells were trypsinised and resuspended in FACS buffer (1ξ Ca2+ and Mg2+ free PBS, 2 mM ethylenediaminetetraacetic acid (Life Technologies), 2% FBS Bovogen) ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was purified using TRIzol reagent (Invitrogen - 15596026), after DNase I treatment with RQ1 RNase-Free DNase (Promega - M6101) ...
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Macropinocytosis mediates resistance to loss of glutamine transport in triple-negative breast cancerbioRxiv - Cancer Biology 2024Quote: ... colonies were washed with PBS (Thermo Fisher, Cat. 14190144) and fixed with 100% methanol (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... Red blood cells were lysed using the ACK lysis buffer (A1049201, Gibco) and mononuclear cells were labeled with CD11b ...
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bioRxiv - Cancer Biology 2024Quote: ... qPCR was then assayed on 0.2 µg of cDNA using Platinum Taq DNA polymerase (Invitrogen-3644073) and specific DNA primers (IDT sequences in table 2) ...
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Macropinocytosis mediates resistance to loss of glutamine transport in triple-negative breast cancerbioRxiv - Cancer Biology 2024Quote: ... incubated with primary antibody (anti-human ASCT2 from MedImmune diluted 1:3000 in FACS buffer) for 30 min and secondary antibody for 20 min (Goat anti-human IgG Fc PE secondary antibody (Invitrogen) diluted 1:250 in FACS buffer) ...
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bioRxiv - Cancer Biology 2024Quote: ... Transfection was performed using Lipofectamine 3000 Transfection Reagent (Thermo Fisher Scientific; cat. L3000015) with pMD2.G (lentiviral helper plasmid ...
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Macropinocytosis mediates resistance to loss of glutamine transport in triple-negative breast cancerbioRxiv - Cancer Biology 2024Quote: ... washed twice with MilliQ water and imaged using the iBright FL1500 Imaging System (Thermo Fisher). Colony number and area were quantified using Fiji (v.1.53q ...
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bioRxiv - Cancer Biology 2024Quote: Mouse ES cells were grown in DMEM medium (Biological Industries) supplied with 20% fetal bovine serum (Gibco), 1% penicillin-streptomycin (Gibco) ...