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Citations for Thermo Fisher :
7651 - 7700 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Cellular RNAs captured by bead-bound antibody complexes were digested to <100 nt by incubating for 30 min at 4°C with RNase I (1 U/μl; Thermo Fisher Scientific Ambion; AM2294). After extensive washing ...
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bioRxiv - Neuroscience 2024Quote: ... were transfected using Lipofectamine RNAiMAX (Invitrogen) according to manufacturer guidelines ...
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bioRxiv - Neuroscience 2024Quote: ... pre-designed siRNAs (Ambion Silencer Select or Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: Cells were harversted in 1mL TRIzol Reagent (Thermo Fisher Scientific) per 1-3 million cells ...
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bioRxiv - Neuroscience 2024Quote: ... Reverse transcription of the adapter-ligated RNAs using SuperScript III reverse transcriptase (Thermo Fisher Scientific) was followed by PCR amplification using Phusion high-fidelity DNA polymerase (New England Biolabs ...
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bioRxiv - Neuroscience 2024Quote: ... Microscopic samples were mounted on glass slides using ProLong Antifade reagent (Thermo Fisher Scientific). Images were captured using an Olympus FV10i microscope with a 60x/1.35 oil immersion objective and analyzed using ImageJ 1.52N software (National Institutes of Health) ...
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bioRxiv - Neuroscience 2024Quote: ... Brain sections were then mounted in Fluoromount (ThermoFisher Scientific, Waltham, MA) and scanned using a Hamamatsu NanoZoomer 2.0 digital slide scanner (Hamamatsu Photonics K.K. ...
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bioRxiv - Neuroscience 2024Quote: ... DNA yield was then measured using Qubit 3 (High Sensitivity DNA Assay, ThermoFisher). Libraries were prepared using 2 ng of ChIP DNA and 10 ng of Input DNA (KAPA Hyper Prep Kit ...
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bioRxiv - Neuroscience 2024Quote: Click-iT™ EdU Cell Proliferation Kit (Invitrogen, C10337) was used to measure cell proliferation as descried in the protocol ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were mounted onto slides (Superfrost Plus) and cover–slipped using ProLong Gold Antifade Reagent (Thermo Fisher Scientific, catalog no. P36982). All steps were performed at room temperature.
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bioRxiv - Neuroscience 2024Quote: ... A BCA protein assay kit (ThermoFisher, catalog number: 23225) was used to determine protein concentration ...
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bioRxiv - Neuroscience 2024Quote: HEK 293 cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM; Gibco, USA) supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 10% Fetal Bovine Serum (FBS; Gibco, USA) in a 37 °C ...
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bioRxiv - Neuroscience 2024Quote: ... the Alexa Fluor 488 goat anti-mouse (A-11029, ThermoFisher) and the AF 647 goat anti-rabbit (111-605-003 ...
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bioRxiv - Neuroscience 2024Quote: ... slides were incubated with both secondary antibodies AF 488 goat anti-mouse (A-11029, ThermoFisher) and AF 647 goat anti-rabbit (111-605-003 ...
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bioRxiv - Neuroscience 2024Quote: Brains were sectioned in the coronal plane using a cryostat (Thermo Scientific, HM 525 NX) at either a thickness of 20 μm on three alternate slide series for validation experiments or a thickness of 30 μm on two alternate slide series for experimental individuals ...
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bioRxiv - Neuroscience 2024Quote: ... embedded in Neg-50 (22-046-511, Fisher Scientific) and kept at -80°C until sectioning ...
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bioRxiv - Neuroscience 2024Quote: ... 488-anti-rabbit (Invitrogen, A11034, 1:500), 488-anti-mouse (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... and 555-anti-mouse (Invitrogen, A21422, 1:500).
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bioRxiv - Neuroscience 2024Quote: ... sections were immunostained with Streptavidin-568 (Invitrogen #S11226) and ...
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bioRxiv - Neuroscience 2024Quote: ... OXTR mRNA levels were quantified using Taqman gene expression kits (OXTR: Rn00563503_m1; GapDH: Rn01775763_g1; Life Technologies) and PCR reagents (Applied Biosystems) ...
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bioRxiv - Neuroscience 2024Quote: ... Brain sections were mounted on superfrost slides and coverslipped with ProLong antifade reagent with DAPI (Molecular Probes). RdLight1 fluorescence was not immuno-enhanced ...
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bioRxiv - Neuroscience 2024Quote: LC-MS/MS analysis for DDA Peptides from each pooled fraction were first spiked with iRT standard peptides (Biognosys AG, Switzerland) and separated using reverse-phase high-performance liquid chromatography (RP-HPLC) on EASY-nLC system (Thermo Fisher Scientific, Bremen, Germany) with a column (75 μm × 150 mm ...
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bioRxiv - Neuroscience 2024Quote: ... CS-disaccharides were resolved on porous graphite column (Hypercarb column; 2.1 × 50 mm, 3 μm; Thermo Fisher Scientific, Bothell, WA, USA) and LC-MS/MS + MRM were performed using a Waters Acquity I-class ultra-performance liquid chromatographic system (UPLC ...
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bioRxiv - Neuroscience 2024Quote: ... at 1:250) paired with (2⁰ Streptavidin-633 (Invitrogen #S21375) 1:500).
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bioRxiv - Neuroscience 2024Quote: ... cells were loaded with 0.5uM Calcein-AM (Invitrogen (USA), C1430 ...
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bioRxiv - Neuroscience 2024Quote: ... To visualize Gi-DREADD-mCherry expression in rCA2 of injected mice sections were stained with 1⁰ rat anti-mCherry (Life Technology #M11217) 1:500 + 2⁰ anti-rat 568 (Invitrogen #A11077) at 1:250 ...
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bioRxiv - Neuroscience 2024Quote: ... In order to visualize AAV-retro positive cells sections were stained for mCherry (1⁰ rat anti-mCherry (Life Technology #M11217) 1:500 + 2⁰ anti-rat 568 (Invitrogen #A11077) at 1:250 ...
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bioRxiv - Neuroscience 2024Quote: ... RNA was extracted using TRIzol reagent (Invitrogen) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... samples were washed and incubated with donkey anti-mouse secondary antibody tagged with Alexa Fluor 647 (Thermo Fisher #A32787) diluted in fresh blocking solution ...
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bioRxiv - Neuroscience 2024Quote: ... Stained brains were washed again and mounted in SlowFade Glass antifade mountant (Thermo Fisher #S36917). Young and old ants were dissected and stained in parallel using the same solutions and under identical conditions.
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bioRxiv - Neuroscience 2024Quote: ... 1:1000 + 2⁰ anti-chicken 488 (Invitrogen #A11039) 1:500 ...
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bioRxiv - Neuroscience 2024Quote: ... and pericytes in the OGD/R model using a Trizol reagent (Invitrogen). The mRNAs of genes from the prediction analysis ...
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bioRxiv - Neuroscience 2024Quote: ... Potassium chloride and magnesium chloride were purchased from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Neuroscience 2024Quote: ... the medium was switched to HBSS without Ca2+ and Mg2+ (Gibco, 14175095). Utilizing a Nikon A1R Confocal Microscope system equipped with a 488nm argon laser and a x60 1.4 NA Plan-Apochromat objective (Nikon Instruments ...
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bioRxiv - Neuroscience 2024Quote: ... A subset of sections was stained for calbindin+ cells (1⁰ rabbit anti-calbindin (Swant #CB38) 1:500 + 2⁰ anti-rabbit-633 (Invitrogen #A21071) 1:500) ...
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bioRxiv - Neuroscience 2024Quote: ... Equal protein amounts (25 µg) were resolved by SDS-PAGE on Bolt™ Bis-Tris Plus pre-cast 4-12% gradient gels (Invitrogen) and transferred to 0.45 mM polyvinylidene fluoride (PVDF ...
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bioRxiv - Neuroscience 2024Quote: ... Donkey anti mouse IgG (1:600; ThermoFisher Scientific: A-21202). Secondary antibodies used in this study were all purchased from ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... except B27 supplement with vitamin A (Gibco, USA)] ...
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bioRxiv - Neuroscience 2024Quote: ... B27 supplement (1x) (ThermoFisher), CHIR99021 (3 µM ...
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bioRxiv - Neuroscience 2024Quote: ... and 7 μL SYBR Green qPCR master mix (Thermo Fisher, cat no 4472908). The following primers were used ...
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bioRxiv - Neuroscience 2024Quote: ... in neural induction media (DMEM/F12, 1% N2 supplement [Gibco, USA] ...
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bioRxiv - Neuroscience 2024Quote: ... composed of a 1:1 ratio of Advanced DMEM/F12 and Neurobasal medium supplemented with neural induction supplement (Thermo Fisher Scientific, USA). To form neurospheres (83) ...
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bioRxiv - Neuroscience 2024Quote: ... specifically goat anti-mouse Alexa Fluor 594 (Thermo Fisher Scientific, USA) at a dilution of 1:400 ...
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bioRxiv - Neuroscience 2024Quote: ... and immunoblotted with the indicated antibodies: mouse anti-p16Ink4a (Invitrogen) at 1:500 ...
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bioRxiv - Neuroscience 2024Quote: ... RNA concentrations were measured using a Nanodrop 2000 (Thermo Fisher). One μg RNA was used for cDNA preparation using High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher ...
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bioRxiv - Neuroscience 2024Quote: ... the sections were incubated with 300 nM DAPI (Invitrogen, USA) for 5 min ...
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bioRxiv - Neuroscience 2024Quote: hiPSCs were differentiated into NSCs using PSC Neural Induction Medium (Thermo Fisher Scientific, USA), following the manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2024Quote: ... coated organoids were returned to the 24-well ultra-low-attachment plates with differentiation media without vitamin A [1:1 mixture of DMEM/F12 and Neurobasal (Gibco, USA), 0.5% N2 supplement ...
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bioRxiv - Neuroscience 2024Quote: ... Secondary antibodies used in this study were all purchased from ThermoFisher Scientific ...