Labshake search
Citations for Thermo Fisher :
7351 - 7400 of 10000+ citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... medium was changed to differentiation medium containing a 1:1 mixture of IntestiCult™ OGM Human Basal Medium (#100-0190) and Advanced Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F12, ThermoFisher Scientific, 12634-028) supplemented with 1% Pen-Strep ...
-
bioRxiv - Immunology 2023Quote: ... Results of the human samples were developed using a SARS-CoV-2 convalescent plasma as primary and a goat anti-human IgG Alexa Fluor 488 secondary antibody (ThermoFisher Scientific #A48276) (2) ...
-
bioRxiv - Cell Biology 2023Quote: ... and C-X-C motif chemokine 11 (CXCL11) ELISAs were performed using basal supernatants collected 48 hours after cytokine treatments using IL-8 Human ELISA Kit (ThermoFisher, Cat# KHC0081) and Human CXCL11/I-TAC Quantikine ELISA Kit (R&D Systems ...
-
bioRxiv - Neuroscience 2023Quote: ... Human brain tissue was homogenised with a Dounce homogeniser and placed in a low protein binding 15mL tube (Thermo Fisher Scientific, 30122216) containing 10mL 1X artificial CSF (pH 7.4 ...
-
bioRxiv - Cell Biology 2023Quote: ... RT-qPCR was performed with the respective human-specific sense and antisense primers and RT-SYBR™ Green PCR Master Mix (Applied Biosystems). Multiwell plates of 384 wells were run on a Viia 7 PCR System (Applied Biosystems) ...
-
bioRxiv - Cell Biology 2024Quote: ... human MSCs were isolated from the UC (Wharton’s Jelly) and expanded with proliferation medium (Minimum essential Medium-α (MEM-α) (Gibco™) supplemented with 10% (v/v ...
-
bioRxiv - Cancer Biology 2024Quote: ... Sections were transferred to gelatinized slides and immunofluorescence staining was performed using a human CD45 antibody (Rat. YAML501.4; Thermo Fisher Scientific, USA), GFP Polyclonal Antibody (A-11122 ...
-
bioRxiv - Neuroscience 2024Quote: ... The respective mouse and human brain sections were subsequently incubated with Invitrogen donkey anti-rabbit Alexa Fluor 488 (Catalog No. A-21206; Invitrogen, Carlsbad, CA) for VGLUT2 ...
-
bioRxiv - Molecular Biology 2024Quote: ... The constructs were used for in vitro transcription and translation using the 1-Step Human High-Yield Mini IVT Kit (Thermo Fisher; 88891) according to the manufacturer’s instruction ...
-
bioRxiv - Pathology 2024Quote: For routine qPCR analyses 100 mg of grey matter from the STR and the CTX (Brodmann’s area 9/10) of human brains (Table 1) was dissected and extracted in 1.5 ml Trizol reagent (ThermoFisher/Life Technologies, 15596026) using a hand-held homogenizer (6 x15 s bursts)(Pro-Scientific ...
-
bioRxiv - Biochemistry 2024Quote: MS/MS searches were carried out using SEQUEST search algorithms against a Uniprot database for humans (supplemented with exogenous sequence for anticipated protein constructs) using Proteome Discoverer (Version 3.0, Thermofisher Scientific, Bremen, Germany). Oxidation of methionine and N-terminal protein acetylation were included as dynamic modifications and carbamidomethylation of cysteine was set as a static modification ...
-
Cellular sialoglycans are differentially required for endosomal and cell-surface entry of SARS-CoV-2bioRxiv - Microbiology 2024Quote: ... CR3022 antibody (10 μg/mL) and 10 μg/mL of goat anti-human IgG (H+L) secondary antibody Alexa Fluor 647 (Invitrogen #A-21445) was pre-incubated for 30 min at 4 °C ...
-
bioRxiv - Bioengineering 2024Quote: Human monocyte-derived macrophages (hMDM) were cultured in Nunc™ MicroWell™ 96-well optical-bottom plates (Thermo Fisher Scientific, Waltham, MA) and left untreated or treated with vehicle (sterile 1X PBS) ...
-
bioRxiv - Bioengineering 2024Quote: ... flow throughs and washes from StEVAC method were suspended in 20 µl of Exosome-human CD81 flow detection beads (Thermo Scientific, 10622D) and incubated at 4°C overnight while rotating ...
-
bioRxiv - Microbiology 2024Quote: ... Upon stabilisation of baseline readings for human plasma experiments plasma was diluted 1:40 in media (RPMI [Thermo Fisher Scientific, U.S.A.] with B27 Supplement [Thermo Fisher Scientific, U.S.A.]), then transferred to the hiPSC-derived cardiomyocytes for 48 hours of treatment either in the presence or absence of 100ng/mL of dexamethasone (Sigma-Aldrich ...
-
bioRxiv - Immunology 2024Quote: ... Cells were stained with Fixable Viability Dye eFluor 780 and the following monoclonal antibodies: TexasRed-PE conjugated anti-human CD3 (clone 7D6, Invitrogen, cat. MHCD0317); PerCP/Cyanine5.5 conjugated anti-human CD4 (clone OKT4 ...
-
bioRxiv - Microbiology 2024Quote: Supernatant collected from human tissues at 48 or 72 hpi after infection with RVFV ZH501 or LAVs (ΔNSs, ΔNSm, MP-12, ΔNSs/ΔNSm) was used to detect total human interferon alpha (IFNα; Invitrogen catalog #BMS216) and human interleukin 29 (IL-29/IFN-λ1 ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... cells stably expressing the human D1R (D1-HEK) (up to pass 35) were maintained in Dulbecco’s modified Eagle’s medium (DMEM, Gibco/Fisher Scientific, Hampton, NH) supplemented with 10% fetal bovine serum (Omega Scientific ...
-
bioRxiv - Immunology 2024Quote: ... The concentration of hIL-1β and hIL-8 were measured using a human Invitrogen ELISA Kit (Thermo Fisher Scientific, Waltham, MA, USA). All the protocols were used according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2024Quote: Cryopreserved primary human hepatocytes were genotyped for PNPLA3 rs738409(G) using TaqMan® SNP Genotyping Assays (Life Technologies, Assay ID C_7241_10, 4351379) according to the manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2024Quote: ... Human slices were routinely stained with anti-NeuN (diluted 1:300 from stock; Rabbit anti-NeuN, Thermo Fisher Scientific PA5-37407, RRID:AB_2554049), visualized with AF488-conjugated anti-rabbit secondary antibody (1:300 from stock ...
-
bioRxiv - Synthetic Biology 2024Quote: Total secreted HSA concentrations of 72 hr yeast HSA expression cultures were quantified via commercially available Albumin Human ELISA Kit (ThermoFisher Scientific #EHALB), following the included protocol ...
-
bioRxiv - Cell Biology 2024Quote: Plasmids encoding human ZFP36 proteins TTP and Brf1: human ZFP36 and ZFP36L1 open reading frames were amplified from the ORFeome cDNA clone library (Invitrogen, Life Technologies) by Phusion PCR (Thermo Fisher Scientific ...
-
bioRxiv - Synthetic Biology 2024Quote: ... F(ab’)2-goat anti-human IgG (H+L) secondary antibody conjugated to horseradish peroxidase (HRP) (1:5000 dilution; ThermoFisher, Cat # A24464), C ...
-
bioRxiv - Bioengineering 2024Quote: ... One μg of plasmid was electroporated into 200,000 human primary myoblasts carrying the Δ52 DMD mutation using the Neon® transfection system (Life Technologies, Invitrogen), following the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2024Quote: ... DD5A)29 and three controls (Ctl1B, Ctl7C, Ctl8F)47 were cultured on human recombinant laminin LN521 (Biolamina) in Essential-8TM medium (Thermo Fisher Scientific) and passaged using Trypl Express (Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2024Quote: ... Cells were counted and plated at a density of 5×104 cells per well in a flat bottom 96-well plate with anti-CD3 anti-CD28 Dynabeads (Dynabeads™ Human T-Expander CD3/CD28 Cat. 11141D, Gibco,FR) and IL-2 (Proleukin ...
-
bioRxiv - Molecular Biology 2024Quote: A549 wildtype or knockout cells were transfected with various human Mtln expressing plasmids (wildtype, N-terminal or internal V5 epitope-tagged) using Lipofectamine LTX (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
-
bioRxiv - Genomics 2024Quote: ... The concentration of endogenous DNA was measured via quantitative real-time PCR using the Quantifiler™ Human DNA Quantification Kit (Applied Biosystems) and methods from prior studies [24].
-
bioRxiv - Immunology 2024Quote: ... Slides were then washed for 5 minutes 7x with PBS 1.0% BSA at RT followed by detection with a goat anti-human IgG (H+L) AF488 secondary antibody (Thermo Fisher A-11013) diluted in PBS + 1.0% BSA for 1 hour at RT ...
-
bioRxiv - Cell Biology 2024Quote: ... one million human iPSCs were thawed and resuspended with 1 mL of Dulbecco’s Modified Eagle Medium F12 (DMEM F12) media (Thermofisher, Catalog No. 12634010) then diluted in 2 mL of warm DMEM F12 in a 15 mL Eppendorf conical tube (Eppendorf ...
-
bioRxiv - Cell Biology 2024Quote: The pTP6-3xFlag-KDM6A was obtained through EcoRI digestion and subcloning into the pTP6 vector59 of the 3xFlag-KDM6A sequence optimized for human expression (purchased from Thermo Fisher Scientific). The cDNA encoding for human ZFX was purchased from Genescript ...
-
bioRxiv - Biophysics 2024Quote: ... and further subcloned into pAAV2 under the control of the human synapsin promoter and flanked by lox71 and lox66 sites using EcoRI and BamHI restriction sites (FastDigest, Thermo Fisher Scientific). The constructs were soma-targeted using the Kv2.1 targeting domain.
-
bioRxiv - Microbiology 2024Quote: ... blocked in 1% milk in 1x TBS and incubated with Human (HIV-IG) (NIH-ARP, 3957) and anti-β-actin mouse (Invitrogen, AM4302) in 1X TBST ...
-
bioRxiv - Immunology 2024Quote: ... 1×105 PBMCs were cultured with serially diluted numbers of Tregs in the presence of anti-CD3/CD28 Human T-Activator Dynabeads™ (1:8 bead:PBMC; Thermo Fisher Scientific) for 3 days ...
-
bioRxiv - Immunology 2024Quote: ... Whole-cell suspensions were centrifuged and resuspended in a complete medium with anti-CD3/CD28 activation beads (DynabeadsTM Human T-Activator CD3/CD28, Thermo Fisher Scientific) at a concentration of 1 µL per 80,000 cells ...
-
bioRxiv - Immunology 2024Quote: ... mouse and human monoclonal antibodies were partially reduced using 5 molar excess Tris(2-carboxyethyl) phosphine hydrochloride (TCEP, Thermo Fisher Scientific, #77720) for 2 hours at 37°C with rotation ...
-
bioRxiv - Bioengineering 2024Quote: ... FISH analyses were carried out by using digoxigenin-labelled DNA [human COT-1 DNA/mouse COT-1 DNA (Invitrogen, Carlsbad, CA, USA) and IGK-BAC (CH17-216K2)] and biotin-labelled DNA [human COT-1 DNA/mouse COT-1 DNA and IGH-BAC (CH17-212P11)] as described previously29 ...
-
bioRxiv - Bioengineering 2024Quote: ... and APC anti-human CD151 antibody (Cat# 350405, Biolegends)) and expression level was measured by Attune NxT Flow Cytometer (Thermo Fisher Scientific). For the bidirectional perturbations using the MS2/PP7 system ...
-
bioRxiv - Cell Biology 2024Quote: ... K562 clones used for the CRISPRi screen was derived from dCas9-KRAS stable cells (parental)21 and the expression of dCas9-KRAS in TRPV1 expressing clones was also confirmed by transducing with sgRNAs targeting CD81 and confirmed with surface staining of APC anti-human CD81 antibody (bioLegend) followed by flow cytometry on an Attune NxT flow cytometer (Thermo Fisher Scientific) at UCSF LCA ...
-
bioRxiv - Bioengineering 2024Quote: TNF-α enzyme linked immunoabsorbent assays (ELISA) were performed on M1 conditioned media and human serum (Supplementary Table 1) following the manufacturer’s protocol (Invitrogen, Cat. No. KHC3011). Serum samples were diluted ten-fold for the assay ...
-
bioRxiv - Cell Biology 2024Quote: Luminex assays were performed using the following kits according to the manufacturer’s instructions: hCG Human ProcartaPlex Simplex Kit (Invitrogen, EPX010-12388-901) and Bio-Plex Pro Human MMP-2 Set (Bio-rad ...
-
bioRxiv - Cancer Biology 2024Quote: ... 1% [v/v] Anti-Anti [Gibco Cat#15240062]) with 10 µM Y-27632 and 4ng/mL thermostable human basic fibroblast growth factor (bFGF, Gibco Cat#PHG0360). The fifth day ...
-
bioRxiv - Cancer Biology 2024Quote: Freshly isolated fibroblasts from KPTI lungs (GFP- or GFP+) or human lung fibroblasts were cultured in DMEM/F-12 (Thermo Fisher, 11330032) with 10% FBS and 1% Pen/Strep ...
-
bioRxiv - Cancer Biology 2024Quote: ... Two SilencerTM Validated siRNAs along with an ON-TARGET Plus Human siRNA smartpool (Horizon Discovery) for each gene of interest were purchased (Invitrogen, Thermo Fisher).
-
bioRxiv - Cell Biology 2024Quote: ... adenovirus-infected human myotubes were washed once with PBS and treated for 40 min with methionine-free growth medium (Thermo Fisher Scientific) supplemented with 2% FBS and 0.8 mM l-leucine to deplete methionine reserves ...
-
bioRxiv - Cancer Biology 2024Quote: Primary human neonatal keratinocytes were isolated as previously described5 and cultured in Medium 154 with Human Keratinocyte Growth Supplement and Penicillin-Streptomycin (ThermoFisher Scientific, USA). CAL27 cells were cultured in DMEM medium (Gibco ...
-
bioRxiv - Cell Biology 2020Quote: ... cells were incubated in Live Cell Imaging Solution (Invitrogen) for 2 hours prior to incubation in experimental nutrient combinations ...
-
bioRxiv - Cell Biology 2020Quote: ... Cells were imaged in Live Cell Imaging buffer (Invitrogen) supplemented with 20⍰mM glucose ...
-
bioRxiv - Neuroscience 2020Quote: ... Cells were maintained in Live Cell Imaging Solution (Invitrogen) at 37°C in 5% CO2 during image acquisition and microscope acquisition parameters for all images were identical ...