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Citations for Thermo Fisher :
7351 - 7400 of 10000+ citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ESCs were maintained on Recombinant Human Protein Vitronectin (Thermo Fischer # A14700) coated plates using mESC maintenance media containing Glasgow Minimum Essential Medium (Thermo Fisher Scientific # 11710035), Embryonic Stem Cell-Qualified Fetal Bovine Serum (Thermo Fisher Scientific # 10439001) ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells stably expressing human recombinant LOXL1 were maintained in culture (37°C, 5% CO2) at log-phase growth in advanced DMEM (Thermo Fisher Scientific, 12491015) with 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Immunology 2023Quote: ... Quantitative gene expression for mouse and human genes was performed using TaqMan probes and TaqMan Fast Advanced Master Mix (Applied Biosystems/Thermo Fisher, 4444554) with a QuantStudio 6 Pro (Applied Biosystems) ...
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bioRxiv - Immunology 2023Quote: ... Freshly isolated CD8+ T cells were differentiated in vitro to CTLs by incubation for 48 h with anti-CD3/CD28 coated magnetic beads (Dynabeads Human T-activator CD3/CD28, Gibco, Waltham, Massachusetts, USA) for cell expansion and activation and 50 U/mL of IL-2 (human Interleukin-2 ...
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bioRxiv - Immunology 2023Quote: The IgG antibodies were extracted from a pool of 100 healthy human serum samples using the Melon™ Gel IgG Spin Purification Kit (Thermo Fisher Scientific), with a tandem purification (one after another ...
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bioRxiv - Immunology 2023Quote: ... cells at the end of 1-day culture were pulsed for 20 minutes with 40 ng/ml of recombinant human IFN-γ (Gibco/Thermo Fisher), 100 IU/ml of recombinant human IL-2 (Roche Diagnostics ...
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bioRxiv - Genomics 2023Quote: ... Cells were rinsed once in staining buffer and incubated with 2 µg/ml anti-human CD326 conjugated with AlexaFluor 700 (ThermoFisher 56-9326-42) at 4C in the dark for 1h ...
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bioRxiv - Microbiology 2023Quote: Isolates from wastewater and isolates provided from collaborators (8 animal/food, 6 environment, 1 human skin, 137 UTI) were grown in tryptic soy broth (TSB; Thermo Scientific; cat. CM0129B) for 24 – 48 hours at 37°C to an OD600 of ∼1 ...
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bioRxiv - Biochemistry 2023Quote: ApoE was immunoprecipitated from matched participant samples of 20 μL CSF and 2 μL plasma via a monoclonal antibody to human apoE (HJ15.3) (provided by the Holtzman Lab) conjugated to M-270 Epoxy Dynabeads (Life Technologies/Invitrogen, Carlsbad, CA). The CSF samples were prepared by combining 20 μL of neat CSF with 100 μL of the apoE3*/apoE4* astrocyte media internal standard ...
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bioRxiv - Immunology 2023Quote: ... the mice received an intravitreal injection of either human monocytes or hMdɸs that were labeled with the Vybrant DiO tracer (Invitrogen-Molecular Probes, Carlsbad, CA), and then returned to the standard light/dark cycle ...
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bioRxiv - Cell Biology 2023Quote: SEES2 ESCs24 were cultured on irradiated MEFs (irrMEFs) with medium for human ESCs: Knockout-Dulbecco’s modified Eagle’s medium (KO-DMEM: 10829-018, Life Technologies; Thermo Fisher Scientific, MA, USA) supplemented with 20% Knockout-Serum Replacement (KO-SR ...
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bioRxiv - Microbiology 2022Quote: ... cells were washed once by Hanks’ Balanced Salt Solution (HBSS) and then incubated with 2 μg/mL of Alexa Fluor 488-conjugated goat anti-human IgG (Thermo Fisher Scientific; A11013) or DyLight 594-conjugated goat anti-human IgG (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2023Quote: Primary human CD4+ T cells expressing CBh-ZIM3-dCas9-Bsd (transduced and selected as described above) were activated with Dynabeads Human T-Activator CD3/CD28 (Thermo Fisher Scientific, 11131D) at 1:1 cell:bead ratio and ...
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bioRxiv - Biophysics 2023Quote: The nucleotide sequence encoding the human ADORA2A (1-316 aa) (UniProt ID C9JQD8) was codon optimized for Leishmania tarentolae expression by the GeneOptimizer software (Thermo Fisher Scientific, USA) and synthesized de novo (Eurofins ...
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bioRxiv - Physiology 2023Quote: ... HEK293 cells stably expressing the human hERG channel (Bioprojet, France) were cultured in the same medium with 400 µg/mL G418 (Thermo Fisher Scientific, France), at 5% CO2 ...
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bioRxiv - Immunology 2023Quote: ... or VHHASC with a C-terminal HA tag or C-terminal EGFP fusion under the control of the human elongation factor-1 α promoter (pEF1α) were generated using lentiviral vectors constructed by Gateway cloning (Thermo Fisher Scientific) using vectors modified from pRLL (a kind gift of Susan Lindquist ...
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bioRxiv - Physiology 2023Quote: Confluent HUVECs were stimulated by 50 ng/ml human VEGF-A for indicated lengths of time and lysed in M-PER buffer (Thermo Fisher Scientific, USA) supplemented with protease and phosphatase inhibitors (Mini Complete/PhosphoStop ...
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bioRxiv - Zoology 2023Quote: ... Supernatants were collected 24 hours post-treatment and then IL-8 secretion was quantified using commercial human IL-8 ELISA kits (Invitrogen, cat. #88-8086) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... were determined by matching the UniProt human protein database (release 2023_01) with the acquired fragmentation pattern using Sequest (Thermo Fisher Scientific, Waltham, MA) (Eng et al. ...
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bioRxiv - Neuroscience 2024Quote: ... The GFP plasmid and GPRC6A-Myc plasmid were transfected into the HEK293T that stably expresses a tetracycline-inducible wild-type human tau (4R0N) (iHEK-Tau) using Lipofectamine™ 2000 Transfection Reagent (Invitrogen™, #11668019). Doxycycline was added to the cells to induce tau production on the first day of transfection ...
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bioRxiv - Molecular Biology 2023Quote: ... RT-PCR samples were prepared using Taqman 1-Step qRT-PCR master mix and Taqman gene expression assays for human TBP (Invitrogen, cat no. Hs00427620_m1). Custom primers and probes were ordered from Genscript to quantify the different splice variants (see Table S1 for sequences and Figure 2 for alignment on the minigene sequence) ...
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bioRxiv - Cell Biology 2023Quote: ... Ovine (Fig. 5) and human Pcells (supplement Fig.5S) were treated with monoclonal mouse SERCA2 antibody (clone 2A7-A1; Thermo Fisher; see supplement) and secondary fluorescent antibody Alexa Fluor® 568 Goat Anti-Mouse IgG (H+L ...
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bioRxiv - Microbiology 2024Quote: ... The human mAb 860-55D against intact capsids was purchased from Mikrogen (Neuried, Germany) and detected using the pAb Alexa Fluor® 488 conjugated goat anti-human (A11013, Thermo Fisher Scientific). The mouse mAb 3113-81C against viral capsid proteins was obtained from US Biological (Boston ...
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bioRxiv - Developmental Biology 2024Quote: ... 5% CO2, and 95% humidity. GFP labeled Human Brain Vascular Pericytes (HBVP) (ScienceCell™, Cat. #: 1200) were grown in DMEM (Gibco, Cat. # 11995065) supplemented with 10% FBS and 1X Antibiotic-Antimycotic under the cell culture conditions indicated above ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were then handled by the University of Colorado Anschutz Genomics and Microarray Core for microarray analysis using a Human Clariom D chip (902922; Applied Biosystems via ThermoFisher). This array has over 540,000 transcripts on it including mRNAs ...
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bioRxiv - Microbiology 2024Quote: ... cells were washed once with Hanks’ Balanced Salt Solution (HBSS) and incubated with 1 μg/mL of Alexa Fluor 488-conjugated goat anti-human IgG (Thermo Fisher Scientific; A11013) diluted in HBSS/1% BSA for 1 hour at 37℃ ...
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bioRxiv - Neuroscience 2024Quote: ... Peptide arrays were washed 3 times for 5 min at RT with TBST and incubated with the antibody Rabbit anti-Human IgG DyLight™ 800 (Cat# SA5-10116, Thermofisher, 1/1000) diluted in TBST ...
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bioRxiv - Biochemistry 2024Quote: ... cells were incubated for 1 hour sat room temperature with the following secondary antibodies: anti-goat anti-human Alexa Fluor 647 (Invitrogen, Carlsbad, California, USA). All washing steps were performed with PHEM-T buffer ...
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bioRxiv - Immunology 2024Quote: ... Goat anti-human IgG conjugated with horseradish peroxidase (HRP) was used as the secondary antibody at a dilution of 1:2000 (Life technologies, Ref H10307) in PBS-T-milk for 1 hour at RT ...
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bioRxiv - Biochemistry 2020Quote: ... Lymphocytes cells were grown in cell culture medium (RPMI) (Invitrogen). All cultured media were supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Cancer Biology 2021Quote: Cells were dissociated into single cells with StemPro Accutase (Gibco). Apoptosis assay was conducted using FITC-Annexin V Apoptosis Detection kit (BD Biosciences ...
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bioRxiv - Cell Biology 2020Quote: ... We measured cell size using Countess automated cell counter (Invitrogen). We measured each cell line at least four times and take average of measured cell sizes ...
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bioRxiv - Neuroscience 2022Quote: ... Cell counts were determined using a cell counter (Invitrogen Countess) and Fc block (BD Biosciences 553142 ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell numbers were counted with Countess cell counting chambers (Invitrogen). Statistical analyses were determined by a Mann-Whitney test (ns p>0.05 ...
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bioRxiv - Molecular Biology 2019Quote: Cells were counted using a Countess automated cell counter (Invitrogen). To determine doubling times for the different cell lines ...
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bioRxiv - Cancer Biology 2020Quote: ... Target cells were typically labeled with Cell Trace Violet (Invitrogen) for 15 minutes in DPBS at 37°C and washed prior to co-culture ...
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bioRxiv - Cell Biology 2021Quote: ... cells were maintained in Live Cell Imaging buffer (Life Technologies) in a cage incubator (Okolab ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were lysed in Cell Extraction Buffer (Thermo Fisher Scientific) supplemented with protease inhibitor cocktail ...
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bioRxiv - Microbiology 2021Quote: ... donor cells were pre-labeled with cell dye eFluor450 (ThermoFisher). For transwell experiments ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were counted using Countess™ Automated Cell Counter (Invitrogen) and approximately 3 × 106 cells per sample were used for barcoding ...
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bioRxiv - Cell Biology 2021Quote: ... cells were plated in satellite cell media [DMEM/F12 (Gibco), 20% FBS (Hyclone) ...
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bioRxiv - Cell Biology 2021Quote: ... cells were counted using Countess Automated Cell Counter (Life Technologies) and 500 viable cells per condition were plated in 100 mm dishes in growth medium ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were dissociated in FACSmax cell dissociation solution (Ambion, T200100) containing papain (30U/mg ...
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bioRxiv - Immunology 2022Quote: ... T cells were first labeled with cell tracker violet (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... High Five insect cells (B85502, ThermoFisher; 1.2–2.4 ×108 cells) were co-transfected with the recombinant viruses of appropriate combinations and were grown at 28°C for 60-72 hr.
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bioRxiv - Developmental Biology 2022Quote: ... Cells were dissociated with Hank’s based cell dissociation buffer (Gibco) for 20 minutes at 37°C ...
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bioRxiv - Bioengineering 2021Quote: ... the cells were dislodged with a cell scraper (ThermoFisher 179693) and pelleted by centrifugation (500x g for 3 minutes) ...
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bioRxiv - Immunology 2021Quote: ... Cell lysates were prepared using Denaturing Cell Extraction Buffer (Invitrogen) containing protease & phosphatase inhibitor cocktail (ThermoScientific) ...
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bioRxiv - Cancer Biology 2020Quote: ... Live-cell imaging of cells in CO2-independent media (Gibco) utilized Nikon Plan Apo 20X or 40X DIC N2 0.75 NA objectives and an environmental chamber at 37°C.
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bioRxiv - Immunology 2022Quote: ... sorted cells were seeded onto Nunc Cell-Culture plates (ThermoFisher) at a density of 1 million cells/ml in Decidua culture medium ...