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Citations for Thermo Fisher :
7601 - 7650 of 10000+ citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Peptide spectrum matching of MS/MS spectra of each file was searched against the human database (UniProt) using the Sequest algorithm within Proteome Discoverer v 2.3 software (Thermo Fisher Scientific, San Jose, CA).
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bioRxiv - Immunology 2022Quote: Human monocytes (20 x 106) were lysed in 650 µl of IP Lysis/Wash buffer (Pierce Direct IP Kit, Thermo Fisher Scientific, Cat. #26148) and the supernatants of cell lysates after centrifugation were transferred to columns containing the TBK1 antibody linked to AminoLink Plus Coupling Resin to pull down the proteins interacting with TBK1 using the Pierce Direct IP Kit according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... We performed confocal reflectance microscopy on unlabeled fibrin samples (see Fig. 2) and fluorescence imaging (see Fig. 4) on networks polymerized from unlabeled fibrinogen and Alexa488-labeled human fibrinogen (Life Technologies, Bleiswijk, the Netherlands) mixed in a 30:1 molar ratio.
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bioRxiv - Cancer Biology 2020Quote: ... Recombinant human IFNγ (285-IF) was purchased from R & D Systems (Minneapolis, MN) and mouse IFNγ (BMS326) from eBioscience (Thermo Fisher Scientific, Waltham, MA). The following antibodies were used for Western blot ...
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bioRxiv - Immunology 2020Quote: ... Cells were washed 4X in PBST and subsequently incubated with secondary antibodies (goat anti-rabbit IgG 488, Invitrogen, Carlsbad, CA; #A-11034, 1:4000; & goat anti-human 647, Invitrogen #A-21445, 1:4000) for 1 hour at room temperature ...
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bioRxiv - Genetics 2020Quote: ... Human DNA was removed using DNase I and RNA concentration was measured using Qubit RNA HS Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA). After human DNA-depletion ...
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bioRxiv - Developmental Biology 2020Quote: ... the ells were transfected with human EC-SOD (hEC-SOD) cDNA inserted into a vector plasmid pcDNA3 (5446 nucleotides; Invitrogen Life Technologies, Carlsbad, CA, USA) as previously described,14 using the FuGENE kit (Roche Diagnostics ...
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bioRxiv - Biophysics 2020Quote: The plasmid pT7-7 containing human aSyn cDNA was transformed into Escherichia coli One Shot® BL21 (DE3) Star™ (Thermo Fisher Scientific, USA). 0.5 L cultures of E ...
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bioRxiv - Immunology 2021Quote: ... Transduction efficiency was determined between days 8-10 of culture using a polyclonal goat anti-human IgG Fc PE (ThermoFisher Scientific, #12-4998-82) and the synaptic transfer to BSLB addressed immediately.
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bioRxiv - Immunology 2021Quote: ... with an N-terminal IL-2 signal peptide for secretion and a Fc part (hinge-CH2-CH3) of human IgG1 for purification was inserted into pFUSE-mIgG1-Fc2 vector (Invitrogen, Thermo Fisher Scientific, MA, USA). The construct (RBD-huFc ...
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bioRxiv - Immunology 2021Quote: ... and then incubated with a 50-fold dilution of PE-conjugated goat anti-human IgM or IgG (both from Thermo Fisher, Waltham, MA, US) for 30 minutes at room temperature ...
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bioRxiv - Immunology 2022Quote: ... genes encoding single chain (sc) Fab and scFc linked to human apoferritin light chain monomers were synthesized and cloned by Geneart (Thermo Fisher Scientific, Waltham, MA) into the pcDNA3.4 expression vector ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were then washed once with 150 μL 1% BSA/PBS and then incubated with 50 μL Goat anti-Human IgG Fc secondary antibody PE (ThermoFisher, cat #12-4998-82) at a final concentration of 2.5 μg/mL in 1% BSA/PBS ...
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bioRxiv - Cell Biology 2024Quote: ... hCMEC/D3 cells were plated in the plates or culture dishes at 6 × 104 cells/cm2 and transfected with 10 nM of negative control or human FOXO1 and ETS1 small interfering RNA (siRNA) (Tsingke Biotechnology, Beijing, China) for 12 h using Lipofectamine™ 3000 (Invitrogen, Carlsbad, CA, USA) reagent according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... PWR-1E and PIN cells were cultured in Keratinocyte Serum-Free Medium supplemented with bovine pituitary extract and recombinant human epidermal growth factor (Gibco, Life Technologies, Grand Island, NY). Myc-CaP ...
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bioRxiv - Immunology 2024Quote: Human monocytes (20 x 106) were lysed in 650 µl of IP Lysis/Wash buffer (Pierce Direct IP Kit, Thermo Fisher Scientific, Cat. #26148) and the supernatants of cell lysates after centrifugation were transferred to columns containing the TBK1 antibody linked to AminoLink Plus Coupling Resin to pull down the proteins interacting with TBK1 using the Pierce Direct IP Kit according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... then 50 μl of sample or standard was added per well (in duplicate) with premixed beads in a 96-well plate according to the manufacturer instructions (ThermoFisher Procartaplex Human Simplex kit). The plate was incubated overnight on a shaker at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... 25 µL of supernatant was analyzed using Cytokine 35-Plex Human Panel for the Luminex™ platform (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA) on the Luminex MAGPIX ...
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bioRxiv - Pathology 2023Quote: ... Residual human IgG in the DDS and in the ocular tissues was determined using goat anti-human IgG antibody (A-21089, Thermo Scientific, Waltham, MA, USA). The total number and area of cells expressing CD45 in corneal sections were determined using the “Analyze Particles” tool in ImageJ software ...
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bioRxiv - Immunology 2023Quote: ... Quantitative gene expression for mouse and human genes was performed using TaqMan probes and TaqMan Fast Advanced Master Mix (Applied Biosystems/Thermo Fisher, 4444554) with a QuantStudio 6 Pro (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5% fetal bovine serum except for RWPE-1 and RWPE-2 cell lines which were grown in a keratinocyte serum-free medium supplemented with 5 ng/mL recombinant human epidermal growth factor (EGF) and 50 μg/mL bovine pituitary extract (Gibco™, Invitrogen, Carlsbad, CA, USA). The cell lines were tested for short tandem repeat (STR ...
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bioRxiv - Immunology 2023Quote: ... The concentrations of plasma cytokines and chemokines were measured via Luminex xMAP technology utilizing the Procartaplex Human Cytokine/Chemokine Convenience Panel 1A 34-plex (Thermo Fisher EPXR340-12167-901) in accordance with the manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: SEES2 ESCs24 were cultured on irradiated MEFs (irrMEFs) with medium for human ESCs: Knockout-Dulbecco’s modified Eagle’s medium (KO-DMEM: 10829-018, Life Technologies; Thermo Fisher Scientific, MA, USA) supplemented with 20% Knockout-Serum Replacement (KO-SR ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were washed with TBS-BSA and then incubated with TBS-BSA and 20 μg/mL Alexa 594-conjugated human transferrin (Thermo Fisher Scientific; cat# T13343) for 1h at RT ...
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bioRxiv - Genomics 2023Quote: ... 5 U/mL, human Chorionic Gonadotropin-hCG (Chorulon®, Intervet International B. V., Boxmeer, Netherland) and 50 μg/mL gentamicin (Gibco Thermo Fisher, Massachusets - USA). Culture droplets were covered with mineral oil and placed in an incubator at 38.5°C and a humid atmosphere of 5% CO2 in atmospheric air ...
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bioRxiv - Pathology 2023Quote: Human VICs (P10462, Innoprot, Bizkaia, Spain) were maintained in Fibroblast Medium (P60108, Innoprot) supplemented with 10% FBS (10270-106, Gibco, Grand Island, NY, USA), sodium pyruvate ...
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bioRxiv - Cancer Biology 2023Quote: ... and incubated at 37°C in Human Complete Feeding Medium (hCPLT) consisting of the following: Advanced DMEM/F12 (Thermo Fisher Scientific, Waltham, MA, USA), HEPES (final concentration ...
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bioRxiv - Microbiology 2023Quote: ... A portion of the Fc blocked-cells were separated to accommodate the unlabeled control while remaining samples were incubated with mouse anti-human HLA-ABC W6/32 (Invitrogen [MA5-11723], 1:40) on ice for 30 min ...
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bioRxiv - Neuroscience 2023Quote: ... in PBS at room temperature in the dark and incubated in the appropriate secondary antibody (Anti-human 488 and Anti-rabbit 647, Invitrogen, both at 1:500) at room temperature for 1 hour while on a rotary shaker (65 rpm) ...
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bioRxiv - Bioengineering 2024Quote: ... HUVECs were washed with media twice and labeled using a mouse primary antibody targeting human ICAM-1 (Thermo Fisher Scientific, Cat. # 14-0549-82) at an appropriate working concentration (1:100 dilution in media ...
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bioRxiv - Bioengineering 2024Quote: ... Briefly hiPSCs were plated on low-attachment U-bottom 96 well plate and cultured in ESCM supplemented with ROCKi (50µM) and 4ng/ml Human FGF-basic Recombinant Protein (Gibco, Thermo Fisher Scientific, Cat no. PHG0024). At day 6 ...
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bioRxiv - Molecular Biology 2024Quote: ... Exons and flanking intronic regions used for pSpliceExpress minigenes were PCR amplified from human male genomic DNA using primers with attB overhangs and subsequently recombined into pSpliceExpress using BP Clonase II (Thermo Fisher, cat no. 11789020)
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bioRxiv - Microbiology 2024Quote: ... and cultured in Gibco® keratinocyte serum-free medium (K-SFM) supplemented with recombinant human EGF and bovine pituitary extract (Thermo Fisher Scientific, #17005075). The cells were propagated in 75cm2 flasks (Falcon ...
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β-1,6-glucan plays a central role in the structure and remodeling of the bilaminate fungal cell wallbioRxiv - Microbiology 2024Quote: ... Complement activation was measured by quantifying deposited C3b upon adding the wells with mouse monoclonal anti-human C3b antibody (MA1-82814, ThermoFisher Scientific, diluted 1:1,000) diluted in PBS 1X/BSA 1% and incubating 1 h at ambient temperature ...
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bioRxiv - Bioengineering 2024Quote: ... Bound ch5D3 was detected by goat anti-human secondary antibody conjugated to Alexa Fluor 647 (1 µg/mL; Thermo Fisher Scientific; cat.no. A-21445). All incubations were carried out 15 minutes at 4°C followed by stringent washes ...
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bioRxiv - Biophysics 2021Quote: Coverslips were coated Cell-Tak (Corning Cell-Tak; Fisher Scientific, Germany) in 0.1 M sodium bicarbonate (Sigma Aldrich ...
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bioRxiv - Immunology 2021Quote: ... unstained cells suspended at 4×106 cells/mL in DMEM (Gibco) with FBS (5% vol/vol ...
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bioRxiv - Immunology 2020Quote: ... cells were resuspended in red blood cells (RBC) lysis buffer (ThermoFisher), mixed gently and incubated for 10min at 37°C and 5% CO2 ...
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bioRxiv - Molecular Biology 2021Quote: ... whole cell lysates were prepared in Cell Extraction Buffer (ThermoFisher Scientific) supplemented with protease inhibitor cocktail P8340 ...
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bioRxiv - Microbiology 2020Quote: ... cells were stained with NucBlue live cell nuclear stain (Life technologies) for 20 minutes at 37ºC and subsequently imaged ...
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bioRxiv - Immunology 2019Quote: ... chicken macrophage cells (HD11 cells) were cultured in RPMI1640 medium (Gibco) complemented with 10% FBS ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were treated with ACK red blood cell lysis buffer (Gibco) and tumor single cell suspensions were prepared for staining and analysis by flow cytometry ...
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bioRxiv - Molecular Biology 2019Quote: ... U87MG cells were detached using Enzyme-Free Cell Dissociation Buffer (Gibco); K562 cells were grown in suspension ...
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bioRxiv - Immunology 2019Quote: ... These cells were then imaged on the Cell Insight NXT (ThermoFisher) at 20X ...
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bioRxiv - Immunology 2020Quote: ... cells were detached with non-enzymatic cell dissociation buffer (13151014, GIBCO). Cells were either incubated directly with primary antibody or fixed and permeabilized prior to antibody addition ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were counted on Countess II automated cell counter (Thermo Fisher) 12,000 cells were loaded per lane onto a 10X Chromium microfluidic chip ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were imaged regularly on the EVOS Cell Imaging System (ThermoFisher) until they reached approximately 300μm in diameter ...
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bioRxiv - Cancer Biology 2019Quote: Cells were labeled with 1 µM Cell-Tracker Green (Invitrogen; C7025) or Cell-Tracker Red (Invitrogen ...
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bioRxiv - Immunology 2019Quote: Cell proliferation was assessed with Cell proliferation Dye eFluor450 (Thermo Fisher). Briefly ...
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bioRxiv - Genomics 2021Quote: ... and cell viability measured from the cells using PrestoBlue reagent (ThermoFisher) by reading fluorescence on a BioTek Synergy HT plate reader ...