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Citations for Thermo Fisher :
7301 - 7350 of 10000+ citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... and treated with Aß-S8C dimer was subjected to hybridization in duplicates on the GeneChip PrimeView Human Gene Expression Array (Affymetrix, Thermo Fisher Scientific) at the BMFZ (Biomedizinisches Forschungszentrum ...
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bioRxiv - Biochemistry 2019Quote: For mammalian expression a DNA polynucleotide encoding the transmembrane domain (residues 1-295) of GtACR1 optimized for human codon usage was cloned into the pcDNA3.1 vector (Life Technologies, Grand Island, NY) in frame with an EYFP tag ...
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bioRxiv - Cancer Biology 2019Quote: ... RWPE1 (human prostate carcinoma) were cultured in serum-free keratinocyte media containing bovine pituitary extract and epidermal growth factor (GIBCO Invitrogen, Carlsband, CA) at 37°C and 5% CO2 ...
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bioRxiv - Cell Biology 2019Quote: Human RBCs (1% in PBS) were double labeled with the lipidic dye R18 and the content marker calcein-AM (Molecular Probes, Life technologies). 20 µl of R18 (1 mg/ml in ethanol ...
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bioRxiv - Cell Biology 2019Quote: ... gondii parasites were maintained by serial passage in confluent human foreskin fibroblast (HFFs, ATCC# SCRC-1041) monolayers in Dulbecco’s Modified Eagle’s Medium (DMEM, Life Technologies-Gibco, Cat# 10569-010), supplemented with 1% (v/v ...
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bioRxiv - Cancer Biology 2019Quote: ... labeled cDNA targets were generated with the Encore® Biotin Module (Nugen) and hybridized to a GeneChip® Human Transcriptome Array 2.0 (Affymetrix, Thermo Fisher) following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: MOLM-13 cell clones cultured on-chip for 72 h were stained for the hematopoietic marker CD45 using a PE-conjugated primary antibody (mouse anti-human CD45-PE, Invitrogen #12-0451-82). Prior to imaging ...
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bioRxiv - Molecular Biology 2020Quote: ... 150 ng of labelled probe was combined with 5 μg of salmon sperm and 10 μg of human Cot1 DNA (Invitrogen, Cat No 15279011). Two volumes of ethanol were added and the probe mix was collected by centrifugation and dried ...
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bioRxiv - Genomics 2021Quote: ... The over-expression construct of human LY6 was created by PCR amplification of cDNA by Phusion® High-Fidelity DNA polymerase (Thermo Fisher Scientific), using the following primers LY-6S-Pac1-S-5’-GGAACGTTAATTAACGCTGAAGTTTGTCTGTGCACTA-3’ ...
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bioRxiv - Cell Biology 2021Quote: ... The iPSCs were cultured on irrMEF with medium for human iPSCs : KnockoutTM-Dulbecco’s modified Eagle’s medium (KO-DMEM: 10829-018, Life Technologies; Thermo Fisher Scientific, MA, USA) supplemented with 20% KnockoutTM-Serum Replacement (KO-SR ...
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bioRxiv - Pathology 2021Quote: ... for 1 week and pepsin (1 mg/ml in 0.1N HCl) was used for antigen retrieval for immunostaining with human COMP (Thermofisher; MA1-20221, 1:100), CHOP (Santa Cruz ...
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bioRxiv - Molecular Biology 2020Quote: ... qRT-PCR was performed using TaqMan gene expression assays (Human-Assay ID Hs00917510_m1; and (Rat-Assay ID Rn01489483_m1; Applied Biosystems, Life Technologies, Carlsbad, CA). Gene expression was quantified by the comparative Ct value ...
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bioRxiv - Microbiology 2020Quote: ... media with 0.05 mg/mL bovine pituitary extract and 5 ng/mL human recombinant epidermal growth factor (Gibco - Thermo Fisher Scientific, MA, USA). All culture conditions contained a 5% antibiotic / antimycotic mixed solution (Nacalai Tesque) ...
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bioRxiv - Neuroscience 2022Quote: ... using a commercial human fetal brain cDNA library containing cDNAs fused to the gal4 activation domain of pEXP-AD502 (ProQuest™, ThermoFisher Scientific, USA), as prey ...
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bioRxiv - Cancer Biology 2022Quote: ... A CRISPR based kinome-wide screening was performed using a lentiviral sgRNA library (LentiArray™ Human Kinase CRISPR Library, Thermo Fisher Scientific, M3775) that knocks out 840 kinase and kinase related genes individually with total number of 3214 sgRNA constructs ...
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bioRxiv - Neuroscience 2021Quote: Lund human mesencephalic (LUHMES) neuronal precursors were grown in T75 flasks in proliferation medium (DMEM/F12 GlutaMAX™ supplement medium (Thermo Fisher Scientific), N2 supplement (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... and the N-terminal peptidase domain of human ACE2 were expressed using the Bac-to-Bac Baculovirus Expression System (Invitrogen, Carlsbad, CA, USA) and purified by Strep-Tactin Sepharose (IBA Lifesciences ...
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bioRxiv - Cancer Biology 2022Quote: ... Panc1 and MiaPaCa2 with RBFOX2 knockdown and non-targeting control were generated using human Affymetrix Clariom D Arrays (Life Technologies, Cat. no. 902922) with the standard input kit run on the Applied Biosystems GeneChip 3000 instrument ...
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bioRxiv - Pathology 2019Quote: Mouse podocytes generated from wild-type and INF2 KO C57BL/6 mice (Supplementary Figure 3) and human podocytes (43) were maintained in RPMI 1640 (ThermoFisher Scientific, Waltham MA) supplemented with 10% fetal bovine serum (ThermoFisher) ...
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bioRxiv - Genomics 2019Quote: ... All primer pairs were tested alone and in multiplexed PCR reactions using 10 ng of TaqMan® Control Human Genomic DNA (Thermo Fisher Scientific) in 10 μl reaction volumes ...
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bioRxiv - Systems Biology 2020Quote: ... The amount of VEGF contained in the thawed medium was measured using the protocols and reagents from the VEGF Human ELISA Kit (BMS277-2, Thermo Fisher Scientific, USA), and the amounts of PEDF were measured using a Human ELISA Kit (RD191114200R ...
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bioRxiv - Immunology 2021Quote: ... 32,33 were maintained by serial 2-day passaging in human foreskin fibroblast (HFF-1, ATCC® SCRC-1041™, American Tissue Culture Collection) monolayers cultured in DMEM (Thermofisher scientific) with 10% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2021Quote: ... HeLa cells in 35-mm dishes were transfected with 100 pmol of each siRNA in the Stealth siRNA library targeting 154 human nuclear proteins (Invitrogen–Thermo Fisher Scientific) using Lipofectamine RNAiMax (Invitrogen–Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2019Quote: ... the VEGF-A amount in the conditioned medium of day 14 was measured using a VEGF-A Human ELISA Kit (Thermo Fisher Scientific, USA). The assay was performed according to the manufacturer’s protocol and absorbance was read at 450nm ...
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bioRxiv - Genetics 2020Quote: ... T cells were activated through the T cell receptor subunit CD3 and the co-stimulator CD28 by the addition of Human T-Activator CD3/CD28 Dynabeads at a 1:2 bead:cell ratio (Life Technologies, cat. no. 111.31D) for 72 hours.
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bioRxiv - Immunology 2020Quote: ... Cells were cultured for 1 week at a density of 106/well in 6 well plates with 50 ng/ml recombinant human macrophage colony stimulating factor (M-CSF) (Life Technologies, Carlsbad, CA), 1x GlutaMAX-I (Life Technologies ...
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bioRxiv - Biochemistry 2020Quote: The cDNA encoding a fusion protein consisting of GST followed by a 3C cleavage site and the human SMAD2 MH2 domain (residues 241–465) was inserted into the MCSI of the pFastBac™ Dual vector (Thermo Fisher Scientific) in the Sal1 and Spe1 restriction sites ...
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bioRxiv - Cancer Biology 2021Quote: Full-length human SETD7 cDNA sequences were amplified from a human mRNA pool generated by RT-PCR using SuperScript II RNase H Reverse Transcriptase (Life Technologies,CA, USA). The SETD7 cDNA was cloned into the EcoRI/BamHI site of a pCDH-CMV-MCS-EF1α -Puro vector (System Biosciences ...
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bioRxiv - Genetics 2021Quote: ... 250 000 cells in 2 ml of medium in a six-well plate were transfected using 12.5 pmol of Silencer Select siRNA against human ADCY9 (Ambion cat # 4390826 ID 1039), Silencer Select siRNA against CETP (Ambion cat 4392420 ID 2933 ...
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bioRxiv - Microbiology 2020Quote: ... Briefly, parasites were maintained in 2% human O+ erythrocytes (Interstate Blood Bank, Memphis, TN) in RPMI-1640 medium (Life Technologies, Grand Island, NY) supplemented with 0.5% Albumax II (Life Technologies) ...
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bioRxiv - Microbiology 2021Quote: ... Cell suspensions prepared by enzyme-free CDS were kept in PBS-5% FCS and incubated on ice for 30 min with rabbit polyclonal anti-human HVEM (R140; 1:500) (Terry-Allison et al., 1998) followed by visualizing HVEM with anti-rabbit AF488 (Life Technologies; 1:200). The size of the epidermal samples mostly allowed only the analysis of nectin-1 expression ...
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bioRxiv - Bioengineering 2022Quote: ... Primary human MSCs (Cyagen Biosciences, Suzhou, China) from bone marrow were cultured in a minimum essential medium alpha (MEM-α, Gibco, Carlsbad, CA) medium with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2022Quote: Paraffin-embedded human lung tissue of control and IPF donors was cut with a Microm HM355S microtome (Thermo Fisher Scientific, Waltham, MA, USA) into serial sections of 3 μm ...
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bioRxiv - Microbiology 2023Quote: ... [72] at 4 °C overnight followed by staining with 2 µg/ml of secondary AlexaFluor647-conjugated Donkey anti-human IgG antibody (ThermoFisher Scientific, OR, USA) at room temperature for 45 min ...
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bioRxiv - Neuroscience 2022Quote: ... HLA-DR Antibody (Monoclonal Rabbit Anti-Human, Citrate Buffer HIER, dilution 1:50 Clone: LN-3, Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA), TMEM119 (Rabbit Anti-Human ...
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bioRxiv - Physiology 2022Quote: Human TRPM6 was either transfected in a bicistronic vector pCINeo/IRES-GFP plasmid or in a pcDNA pDEST53 plasmid (ThermoFisher Scientific, Waltham, MA) (26 ...
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bioRxiv - Molecular Biology 2022Quote: Human adult epidermal keratinocytes were cultured in EpiLife medium supplemented with 10% Human Keratinocyte Growth Supplement (HKGS) and 1% Penicillin and Streptomycin at 37℃ in 5% CO2 (all from ThermoFisher Scientific, Waltham, MA). Calcium concentration was increased to 1.5 mM in the medium to induce cell differentiation ...
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bioRxiv - Bioengineering 2022Quote: Epidermal growth factor receptor (EGFR) protein expression levels on the surface of Gli36-derived EVs were quantified using an EGFR Human ELISA kit (ThermoFisher Scientific, Waltham, MA). EVs were spiked in healthy donor serum at different concentrations ranging from 0 to 1.0E11 particles/mL while maintaining the serum-derived EV concentration at 1.0E9 particles/mL ...
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bioRxiv - Cancer Biology 2022Quote: Expression levels of 754 miRNAs were quantified using the TaqMan Human MicroRNA Array A+B Card Set v3.0 (Applied Biosystems, Foster City, CA).
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bioRxiv - Neuroscience 2024Quote: ... The GFP plasmid and GPRC6A-Myc plasmid were transfected into the HEK293T that stably expresses a tetracycline-inducible wild-type human tau (4R0N) (iHEK-Tau) using Lipofectamine™ 2000 Transfection Reagent (Invitrogen™, #11668019). Doxycycline was added to the cells to induce tau production on the first day of transfection ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA of human TAF2 was amplified by PCR with gene-specific primers fused to attB recombination sequences for Gateway® cloning (Thermo Fisher Scientific). The amplified sequence was recombined via BP reaction into the pDONR201 donor resulting in pENTR-TAF2 for N-terminal tagging ...
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bioRxiv - Molecular Biology 2024Quote: ... crude and enriched synaptosomal preparations and surgically resected human brain samples was extracted using TRIzol Plus RNA Purification Kit and Phasemaker Tubes Complete System (Invitrogen, cat. no. A33254). The remaining gDNA was removed using TURBO DNA-free Kit (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... were determined by matching the UniProt human protein database (release 2023_01) with the acquired fragmentation pattern using Sequest (Thermo Fisher Scientific, Waltham, MA) (Eng et al. ...
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bioRxiv - Cell Biology 2024Quote: Plasma TPO levels were estimated in plasma samples using Human PracartaPlex Mix&Match 4-Plex Luminex kit (ThermoFisher Scientific, Cat # PPX-04-MX323UK). Assays were performed as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... 70% confluent EnSC monolayers in 6-well plates were downregulated overnight in 2% DCC-FBS/DMEM/F12 media and transfected with 50nM Stealth siRNA against human GIPC1 or APPL1 (Fisher Scientific, Loughborough, U.K.). For depletion of receptor expression ...
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bioRxiv - Cancer Biology 2024Quote: Genomic DNAs were isolated from human lung tumors and their matched normal tissues using the PureLink Genomic DNA Purification Kit (Invitrogen, Carlsbad, CA, USA), and subjected to semi-quantitative PCR using the primers specific for the microsatellite markers D8S1786 and D8S1752 that straddle the PDLIM2 genetic locus or the PDLIM2 genetic locus itself ...
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bioRxiv - Immunology 2024Quote: ... The resulting human chimeric heavy and light chain genes were separately cloned in pJET 1.2/blunt vector ( Thermo Fisher Scientific, Waltham, MA, USA). The insertion of DNA encoding His-tag was performed using appropriate primers (Table 1 ...
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bioRxiv - Cancer Biology 2024Quote: ... The following siRNAs were used: control siRNA that have no homology to any sequence in the human genome were used as the controls (Thermo Fisher Scientific, #4390846); PFKFB3 siRNAs (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: Reactivity of recombinant S proteins from Wuhan and VOC Omicron as well as of recombinant RBD (Wuhan, Delta and Omicron) to large set (>9000) of human proteins was assessed by using protein microarrays (ProtoArray®, Invitrogen, Thermo Fischer). First microarray chips were blocked by incubation for 1 hour at 4 °C with buffer containing ...
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bioRxiv - Cell Biology 2023Quote: ... Internalization of Alexa Fluor 488-conjugated human α-syn PFFs (1 μM) and epidermal growth factor (EGF; 4 μg/mL; Thermo Fisher Scientific) were assessed similarly ...