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Citations for Thermo Fisher :
7301 - 7350 of 10000+ citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Human iPSCs were plated onto 6-well dishes coated with Matrigel (Thermo-Fisher Scientific; Waltham, MA, USA; Cat. No. 08-774-552) in mTeSR1 medium (STEMCELL Technologies ...
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bioRxiv - Molecular Biology 2022Quote: Keratinocytes were transfected with either HOXC13-AS siRNA or control siRNA for 24 hours (n=3 per group) and transcriptomic profiling was performed using human Clariom™ S assays (ThermoFisher Scientific) at the core facility for Bioinformatics and Expression Analysis (BEA ...
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bioRxiv - Developmental Biology 2022Quote: ... or miR-483-3p 2⍰-O-Methyl antagonist (anti-human; 0, 50 and 100⍰nM in HepG2) using the Lipofectamine RNAiMAX Transfection Reagent (ThermoFisher Scientific – 13778100). Cells were harvested and the luciferase assay was performed as previously described21 using the Dual-Luciferase Reporter Assay System (Promega – E1910) ...
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bioRxiv - Bioengineering 2022Quote: ... MDTs were exposed to a neutral culture medium or to a 20 ng/ml of TNF solution (Recombinant TNF alpha human; 300-01A, PeproTech, Thermo Fisher Scientific) in culture medium for either 30 minutes or 240 minutes ...
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bioRxiv - Physiology 2024Quote: ... at an effector/target ratio of 2:1 in the presence of mouse anti-human CD107a-FITC (11-1079-42; Thermo Fisher Scientific). Cocultures between CTLs and EndoC-βH1 cells expressing HLA-A2 were performed at 37 °C for 1.5 h in Iscove’s Modified Dlbecco’s Medium (IMDM ...
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bioRxiv - Cell Biology 2024Quote: ... The following primary antibodies were used (all at 1:100 dilution in immunofluorescence, 1:1000 in immunoblots): CX36 mouse anti-human (Invitrogen, clone 1E5H5), rabbit recombinant ANTI-FLAG M2 antibody (Invitrogen 710662) ...
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bioRxiv - Developmental Biology 2024Quote: The targeted transcriptome sequencing was performed on the Ion S5XL Next Generation Sequencing system with the Ion AmpliSeq Transcriptome Human Gene Expression Assay (ThermoFisher Scientifics Inc). This assay covers 20,802 human RefSeq genes (>95% of UCSC refGene ...
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bioRxiv - Microbiology 2024Quote: ... falciparum B11 line (Perrin et al., 2018) was maintained at 37°C in human RBCs in RPMI 1640 containing Albumax II (Thermo Fisher Scientific) supplemented with 2mM L-glutamine ...
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bioRxiv - Molecular Biology 2024Quote: ... The diluted pre-amplified products were then utilized directly for real-time PCR reactions according to the manufacturer’s protocol (Applied Biosystems™ TaqMan™ Array Human MicroRNA A+B Cards Set v3.0, ThermoFisher Scientific). For the data analysis ...
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bioRxiv - Synthetic Biology 2024Quote: ... were washed three times in cold flow buffer and then the secondary antibody (Goat anti-Human IgG H+L Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488, ThermoFisher Scientific) at a dilution of 1:500 was added ...
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bioRxiv - Molecular Biology 2024Quote: ... siRNA-resistant human FOXK1 and FOXK2 cDNAs were synthesized into a pBluescript plasmid (Biobasic®) and subcloned into pENTR (Life technologies®). GST- ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA was extracted from these transfected cells and sent to the Bioinformatics and Expression Analysis (BEA) core facility at Karolinska Institutet for Human Clariom™ S assay (ThermoFisher Scientific). Briefly ...
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bioRxiv - Immunology 2023Quote: The coding sequence of wild-type human CXCR4 was synthesized by General Biol (Chuzhou, China) and was cloned into the pFastBac transfer plasmid (Invitrogen, Carlsbad, CA). Heat-stabilized BRIL ...
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bioRxiv - Cell Biology 2024Quote: Primary or secondary antibodies were purchased from the following vendors: rabbit anti-human ROBO1 (PA5-29917, Thermo Fisher Scientific, Rockford, IL, USA); rabbit anti-human ROBO1 (HPA052968 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... from Illumina and the Axiom™ Genome-Wide Human Origins (HO) and the Axiom™ Precision Medicine Diversity Array Plus Kit (PMDA) from ThermoFisher Scientific ...
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bioRxiv - Genomics 2024Quote: ... of the human BPTF was amplified by PCR and subsequently cloned into the pDEST-566 vector using Gateway technology (Thermo Fisher Scientific). The resulting 6x-MBP-hBPTF (701-1073 ...
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bioRxiv - Immunology 2024Quote: ... washed in PBS/T and then incubated with 50 µL γ-chain specific goat anti-human IgG-alkaline phosphatase (AP) (Thermo Fisher) at a 1/2000 dilution for 1 h at RT ...
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bioRxiv - Cell Biology 2024Quote: Knockdown of RORα in human cells was performed using the siGenome SMARTpool Human RORA (#6095) siRNA from the Horizon Discovery “Dharmacon” portfolio (#M-003440-01-005) and Lipofectamine 2000 (Invitrogen, #11668-030). The siRNA stock was diluted to 20 µM in RNase-free water following the product protocols ...
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bioRxiv - Microbiology 2023Quote: ... the first-strand human cDNA was obtained using oligo(dT) primers and Moloney murine leukemia virus-reverse transcriptase (MMLV-RT kit; Life Technologies, USA), and The SuperScript™ VILO™ cDNA Synthesis Kit ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were then measured by using human Aβ1-42 enzyme-linked immunoassay (ELISA) kit (Catalog No. KHB3441, Invitrogen by Thermo Fisher Scientific).
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bioRxiv - Physiology 2023Quote: ... at the final concentration of 5 µM and one of the following anti-human mouse monoclonal antibodies (5 µl per sample) conjugated with allophycocyanin (APC): anti-CD9 (clone MEM-61, Thermo Fisher Scientific), anti-CD63 (clone MEM-259 ...
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bioRxiv - Neuroscience 2022Quote: ... Human LRRK2 G2019S and LRRK2 Knock-out (KO) iPSC lines were generated in human iPSCs obtained from a female clone from Thermo Fisher (#A18945). Sequence information for generating targeting gRNA ...
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bioRxiv - Microbiology 2023Quote: ... falciparum B11 line (Perrin et al., 2018) was maintained at 37◦ C in human RBCs in RPMI 1640 containing Albumax II (Thermo Fisher Scientific) supplemented with 2 mM L-glutamine ...
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bioRxiv - Microbiology 2022Quote: ... the cells were washed two times with FACS buffer to remove any nonbounded Fc-hDectin1 and resuspended in FACS buffer containing a 1:200 dilution of the secondary antibody F(ab’)2-Goat anti-Human IgG conjugated to AlexaFluor™ 488 (ThermoFisher Scientific). This solution was incubated for 1 hour on ice before finally washing twice with FACS buffer and measuring the fluorescence intensity of 5 000 cells using a Guava® flow cytometer with 488nm excitation and fluorescence emission selected using a 522/30 bandpass filter.
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bioRxiv - Microbiology 2023Quote: Levels of IFN-λ in the culture supernatants were determined with ELISA using the human IL-29 ELISA kit (Invitrogen, cat. BMS2049) following the instructions of the manufacturers ...
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bioRxiv - Cell Biology 2023Quote: Luminex assays were performed using the following kits according to the manufacturer’s instructions: hCG Human ProcartaPlex Simplex Kit (Invitrogen, EPX010-12388-901), Bio-Plex Pro Human Inflammation Panel 1 IL-28A / IFN-λ2 (Bio-rad ...
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bioRxiv - Microbiology 2023Quote: ... The pooled libraries were mixed with virus-specific 161 biotinylated probes in the presence of human Cot-1 DNA (Invitrogen, Cat#15279011) and xGen Universal Blocking Oligos (IDT ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Stable cell lines were transfected with an expression vector containing the full-length cDNA coding for specific human sodium channel α-subunit using Lipofectamine (Thermo Fisher). The NaV1.x stable cell lines and accessory constructs used correspond to the following GenBank accession numbers ...
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bioRxiv - Biochemistry 2023Quote: ... Detection of bound Af2-IL was performed using a rat anti-human IL-2 mAb Alexa Fluor488 conjugate (Invitrogen, Carlsbad CA, USA). Cell nuclei were counterstained with DAPI (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... These tissues were stained with Abs against human CD4 pre-labeled with Alexa Fluor 488 using an Ab labeling kit (Molecular Probes, A20187), human/mouse Foxp3 (clone 1054c ...
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bioRxiv - Cancer Biology 2023Quote: Knockdown of PR130 in murine and human PDAC cells was performed by transfecting 30 pmol of validated siRNA molecules targeting the PPP2R3A/Ppp2r3a mRNAs (murine: Santa Cruz, sc-108917, 10 µM stock solution; human: Thermo Fisher Scientific ...
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Cryo-electron tomography of eel sperm flagella reveals a molecular “minimum system” for motile ciliabioRxiv - Cell Biology 2023Quote: ... Danio rerio (zebrafish), Mus musculus (mouse), and human proteomes using the CPFC (Trudgian et al., 2010) or Proteomics Discoverer (Thermo Fisher Scientific) programs ...
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bioRxiv - Bioengineering 2023Quote: ... To create SiRPα-Fc fusion soluble proteins the cDNA sequence of the full extracellular domain (aa 27-371) of human wild-type allele 1 SiRPα (wtSiRPα) cDNA (Uniprot: P78324) was codon-optimized and synthesized by GeneArt (Thermo Fisher Scientific), fused with the cDNA sequence of the human hinge and IgG1 heavy chain Fc region (pFUSE-hIgG1-Fc1 ...
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bioRxiv - Evolutionary Biology 2023Quote: The region of the elephant shark genome homologous to human CNE1426 was amplified by PCR and subcloned pCR™8/GW/TOPO® (Invitrogen) (the primer sequences are in Extended Data Table 1) ...
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bioRxiv - Immunology 2023Quote: ... the cells were washed twice with PBS + 1% BSA and stained with 10 μg/mL Alexa Fluor™ 647 Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody (ThermoFisher Scientific) diluted in PBS with 1% BSA ...
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bioRxiv - Biochemistry 2023Quote: ApoE was immunoprecipitated from matched participant samples of 20 μL CSF and 2 μL plasma via a monoclonal antibody to human apoE (HJ15.3) (provided by the Holtzman Lab) conjugated to M-270 Epoxy Dynabeads (Life Technologies/Invitrogen, Carlsbad, CA). The CSF samples were prepared by combining 20 μL of neat CSF with 100 μL of the apoE3*/apoE4* astrocyte media internal standard ...
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bioRxiv - Bioengineering 2023Quote: ... isolated naïve CD8+ T cells were plated at 2.5 × 105 cells/well in a 24-well plate in 2 ml cRPMI on day 0 and activated with DynaBeads Human T-Activator anti-CD3/CD28 coated microbeads (Thermo Fisher Scientific) and recombinant human IL-2 (rIL-2 ...
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bioRxiv - Neuroscience 2022Quote: ... RNA quality and quantity were assessed and samples with RINS of 7 or greater were deemed suitable for further analyses with the Affymetrix Human Exon 1.0 ST Array according to the manufacturer’s instructions (Affymetrix, Santa Clara, CA, USA). After hybridisation ...
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bioRxiv - Cell Biology 2023Quote: ... E6 medium was changed daily up to 4 days before switching to human endothelial serum free medium (hESFM) (Thermo Fisher Scientific, 11111) supplemented with 10nM bFGF (Fibroblast growth factor ...
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bioRxiv - Cell Biology 2022Quote: ... human FLOT2 (ON-TARGETplus Human FLOT2 siRNA SMART POOL, L-003666-01-0010) (Horizon Discovery Ltd.), non-targeting control siRNA (#4390843, 40 nM) (Thermo Fisher Scientific). In brief ...
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bioRxiv - Immunology 2023Quote: ... the mice received an intravitreal injection of either human monocytes or hMdɸs that were labeled with the Vybrant DiO tracer (Invitrogen-Molecular Probes, Carlsbad, CA), and then returned to the standard light/dark cycle ...
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bioRxiv - Cell Biology 2023Quote: ... cells were washed five times with PBS and labeled with secondary antibodies (goat anti-rabbit/human conjugated to Alexa Fluor 647; 1:1000; Invitrogen, #A21246/A21445) and FluoTag-X4 anti-GFP conjugated to Atto488 (1:500 ...
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bioRxiv - Cancer Biology 2023Quote: ... were cultured in in K-SFM + Human recombinant Epidermal Growth Factor (rEGF) and Bovine Pituitary Extract (BPE) supplemented with 1% penicillin and streptomycin (all from Thermo Fisher Scientific). PCa cell line PC-3 (CRL-1435) ...
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bioRxiv - Cell Biology 2023Quote: ... seeding media was exchanged for fresh media containing 10% human serum from pregnant or non-pregnant donors together with EdU (Click-iT™ EdU Proliferation Assay for Microplates, ThermoFisher Scientific) and cultured for 1 week ...
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bioRxiv - Cancer Biology 2023Quote: ... the sections were stained with an anti-human CD4 Ab that was pre-labeled with Alexa Fluor 488 using an Ab labeling kit (Molecular Probes, A20187), anti-human/mouse Foxp3 Ab pre-labeled with Alexa Fluor 555 (Molecular Probes ...
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bioRxiv - Microbiology 2023Quote: ... ATCC TIB-202) or primary human monocytes (PHMs) were propagated in RPMI 1640 with L-glutamine and 25 mM HEPES buffer (Invitrogen, Carlsbad, CA), supplemented with 10% fetal bovine serum ...
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bioRxiv - Bioengineering 2023Quote: ... Cryopreserved hiPSC-derived endothelial cells were thawed in a water bath at 37 °C and transferred into a 15 mL tube containing 7 mL of Human Endothelial SFM medium (11111044; Thermo Fisher Scientific). The cells were centrifuged at 300 x g for 3 min and the pellet was resuspended in Human Endothelial SFM complete medium ...
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bioRxiv - Physiology 2023Quote: ... 0.5-1 µg of total RNA was isolated from human islets and reverse transcribed using an M-MLV RT kit (Invitrogen, MA, USA). TaqMan primers (Applied Biosystems ...
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bioRxiv - Immunology 2023Quote: ... plates were washed and a 1:1000 dilution of goat anti-human IgG (γ-chain specific) alkaline phosphate conjugate antibody (A3187, Thermo Fisher) was added and incubated for 1 h ...
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bioRxiv - Immunology 2023Quote: ... were resuspended in 1 ml medium per well of a 24-well plate and stimulated with Dynabeads Human T-Activator CD3/CD28 (Thermo Fisher Scientific), IL-7 (Peprotech ...