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Citations for Thermo Fisher :
7501 - 7550 of 10000+ citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: The nucleotide sequence encoding the human ADORA2A (1-316 aa) (UniProt ID C9JQD8) was codon optimized for Leishmania tarentolae expression by the GeneOptimizer software (Thermo Fisher Scientific, USA) and synthesized de novo (Eurofins ...
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bioRxiv - Physiology 2023Quote: ... HEK293 cells stably expressing the human hERG channel (Bioprojet, France) were cultured in the same medium with 400 µg/mL G418 (Thermo Fisher Scientific, France), at 5% CO2 ...
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bioRxiv - Immunology 2023Quote: ... or VHHASC with a C-terminal HA tag or C-terminal EGFP fusion under the control of the human elongation factor-1 α promoter (pEF1α) were generated using lentiviral vectors constructed by Gateway cloning (Thermo Fisher Scientific) using vectors modified from pRLL (a kind gift of Susan Lindquist ...
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bioRxiv - Bioengineering 2023Quote: Whole blood was obtained from healthy human donors by venipuncture and collected in BD Vacutainer™ plastic blood collection tubes with K2 EDTA: Hemogard™ Closure (Thermo Fisher) to prevent coagulation following an approved protocol from the IRB at the University of Colorado Boulder (22-0175) ...
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bioRxiv - Zoology 2023Quote: ... Supernatants were collected 24 hours post-treatment and then IL-8 secretion was quantified using commercial human IL-8 ELISA kits (Invitrogen, cat. #88-8086) according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2023Quote: Confluent HUVECs were stimulated by 50 ng/ml human VEGF-A for indicated lengths of time and lysed in M-PER buffer (Thermo Fisher Scientific, USA) supplemented with protease and phosphatase inhibitors (Mini Complete/PhosphoStop ...
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bioRxiv - Molecular Biology 2023Quote: ... RT-PCR samples were prepared using Taqman 1-Step qRT-PCR master mix and Taqman gene expression assays for human TBP (Invitrogen, cat no. Hs00427620_m1). Custom primers and probes were ordered from Genscript to quantify the different splice variants (see Table S1 for sequences and Figure 2 for alignment on the minigene sequence) ...
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bioRxiv - Cell Biology 2023Quote: ... Ovine (Fig. 5) and human Pcells (supplement Fig.5S) were treated with monoclonal mouse SERCA2 antibody (clone 2A7-A1; Thermo Fisher; see supplement) and secondary fluorescent antibody Alexa Fluor® 568 Goat Anti-Mouse IgG (H+L ...
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bioRxiv - Neuroscience 2024Quote: ... Peptide arrays were washed 3 times for 5 min at RT with TBST and incubated with the antibody Rabbit anti-Human IgG DyLight™ 800 (Cat# SA5-10116, Thermofisher, 1/1000) diluted in TBST ...
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bioRxiv - Immunology 2024Quote: ... Goat anti-human IgG conjugated with horseradish peroxidase (HRP) was used as the secondary antibody at a dilution of 1:2000 (Life technologies, Ref H10307) in PBS-T-milk for 1 hour at RT ...
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bioRxiv - Microbiology 2024Quote: ... The human mAb 860-55D against intact capsids was purchased from Mikrogen (Neuried, Germany) and detected using the pAb Alexa Fluor® 488 conjugated goat anti-human (A11013, Thermo Fisher Scientific). The mouse mAb 3113-81C against viral capsid proteins was obtained from US Biological (Boston ...
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bioRxiv - Biochemistry 2024Quote: ... cells were incubated for 1 hour sat room temperature with the following secondary antibodies: anti-goat anti-human Alexa Fluor 647 (Invitrogen, Carlsbad, California, USA). All washing steps were performed with PHEM-T buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were then handled by the University of Colorado Anschutz Genomics and Microarray Core for microarray analysis using a Human Clariom D chip (902922; Applied Biosystems via ThermoFisher). This array has over 540,000 transcripts on it including mRNAs ...
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bioRxiv - Developmental Biology 2024Quote: ... 5% CO2, and 95% humidity. GFP labeled Human Brain Vascular Pericytes (HBVP) (ScienceCell™, Cat. #: 1200) were grown in DMEM (Gibco, Cat. # 11995065) supplemented with 10% FBS and 1X Antibiotic-Antimycotic under the cell culture conditions indicated above ...
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bioRxiv - Microbiology 2024Quote: ... The immune complexes were then either incubated with anti-human IgG Alexa Fluor 647 (Invitrogen; 1:400, incubated for 1h at 4°C) to determine the antibody binding levels or guinea pig complement (Sigma ...
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bioRxiv - Neuroscience 2024Quote: ... RT-PCR samples were prepared using Taqman 1-Step qRT-PCR master mix and Taqman gene expression assays for human TBP (Invitrogen, cat no: Hs00427620_m1) and human PRNP (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... goat anti-mouse Alexa Fluor 488 (1:500, A-11001), and goat anti-human Alexa Fluor 647 (1:500, A-21445) all from Thermo Fisher (Waltham, USA).
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bioRxiv - Microbiology 2024Quote: ... were maintained within human erythrocytes (typically Group O+, 4% haematocrit) in RPMI 1640 medium containing 25 mM HEPES and GlutaMAXTM (ThermoFisher Scientific Cat. # 72400120), and supplemented with 11 mM (additional ...
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bioRxiv - Cell Biology 2024Quote: ... and a combination of 18 µg/mL recombinant human Laminin 521 (Biolamina, Cat. # LN521) and 5 µg/mL Fibronectin (Gibco, Cat. # 33016-015). The Matrigel coating consisted of a 1:52 dilution of growth factor-reduced Matrigel (Corning ...
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bioRxiv - Microbiology 2024Quote: ... cells were washed once with Hanks’ Balanced Salt Solution (HBSS) and incubated with 1 μg/mL of Alexa Fluor 488-conjugated goat anti-human IgG (Thermo Fisher Scientific; A11013) diluted in HBSS/1% BSA for 1 hour at 37℃ ...
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bioRxiv - Pathology 2024Quote: ... Primary antibody used was 1:400 rabbit anti-human IBA1 (Wako 019-19741, Richmond, VA) with secondary antibody 1:450 anti-rabbit Alexa Fluor 488 (Invitrogen A21206, Waltham, MA). Immunofluorescence was analyzed and images acquired using a Zeiss LSM780 laser scanning confocal microscope ...
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bioRxiv - Molecular Biology 2024Quote: Human NRL constructs in pcDNA™4/HisMax vector containing an N-terminal Xpress tag (Cat. No. V86420, ThermoFisher Scientific, Waltham, MA, USA) or in pGEX-4 T2 plasmid containing an N-terminal GST tag (GE Healthcare/Cytiva ...
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bioRxiv - Molecular Biology 2024Quote: ... Raw data files were searched against bovine, mouse or human protein sequence databases (National Center for Biomedical Information, NCBI) using Proteome Discoverer 1.4 software (Thermo Scientific, Waltham, MA, USA) based on SEQUEST algorithm ...
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bioRxiv - Molecular Biology 2024Quote: ... The neuronal media was replaced by a fresh working solution containing 0.25 μM Deep Red Nucleic Acid Stain and 2 μM Calcein Amin in neuronal imaging media (Hibernate A Low Fluorescence, TransetYX, #256) and nucleus of human motor neurons was also stained with Hoechst dye (Thermo Fisher Scientific, #H1399). After 30 min of incubation ...
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bioRxiv - Immunology 2024Quote: ... serially-diluted and co-cultured with allogeneic HLA-A2− PBMCs that were labelled with CPD-eFluor™450 and activated with Dynabeads™ Human T-Expander CD3/CD28 (1:8 bead:PBMC ratio; Thermo Fisher Scientific). Cells were stained and analysed by flow cytometry after 4-days of culture ...
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bioRxiv - Cell Biology 2024Quote: Human WTC11 iPSCs expressing doxycycline-inducible NGN2 expression cassette in AAVS1 locus and pC13N-dCas9-BFP-KRAB targeted to human CLYBL locus were cultured in Essential 8 Medium with E8 supplement (E8 medium) (Thermo Fisher Scientific; A1517001) on Matrigel (Falcon Corning ...
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bioRxiv - Bioengineering 2024Quote: ... 40 ng/µL of plasmid DNA was added to a 1-Step Human Coupled IVT Kit (catalog number: 88881, Thermo Fisher Scientific, USA) according to the kit specifications ...
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bioRxiv - Biochemistry 2024Quote: ... cells were washed once with Hanks’ Balanced Salt Solution (HBSS) and incubated with 1 μg/mL of Alexa Fluor 488-conjugated goat anti-human IgG (Thermo Fisher Scientific; A11013) and Hoechst 33342 (1:10,000 dilution in HBSS ...
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bioRxiv - Cancer Biology 2024Quote: ... The lysate was precleared with IgG for 3-4h at 4 degrees before the overnight incubation with human TROP2 antibody (Invitrogen, 14-6024-82) or mouse TROP2 (R&D ...
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bioRxiv - Bioengineering 2024Quote: ... the media was replaced with 100 µL of fresh 2:1 mixed media supplemented with 100 ng mL−1 recombinant human vascular endothelial growth factor (Thermo Fisher Scientific PHC9394). The samples were cultured for another 2 days before fixation with 40 mg mL−1 formaldehyde in PBS for 40 min and then washed 3 times with PBS ...
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bioRxiv - Cell Biology 2024Quote: IMR-90 primary human lung fibroblasts (ATCC, Manassas, VA; #CCL186) were cultured in Dulbecco’s Modified Eagle Medium (Thermo Fisher Scientific, Waltham, MA; #12430054) supplemented with penicillin-streptomycin (5,000 U/mL ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were then resuspended in a 100 μL solution of PBS containing an antibody targeting human CD45 conjugated to PE-Cy7 (Invitrogen, 25-9459-42) and incubated for 30 minutes in the dark at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... ¼ of cell volume was added to 20 µl dilution buffer with 0.5 µl DNARelease additive from Phusion Human Specimen Direct PCR Kit (Thermo Scientific, Cat #F-150BID) before incubation at 98°C for 2min ...
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bioRxiv - Genetics 2021Quote: ... 500 µl of cell suspensions (5×105 cells/ml) were transferred to cell chambers (Invitrogen A-7816) containing coverslips treated with 0.25mg/ml concanavalin A (Sigma-Aldrich C0412 ...
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bioRxiv - Cancer Biology 2021Quote: ... vital and dead cells were counted with a cell counter (Countess™ II Automated Cell Counter, Invitrogen) using Trypan-Blue exclusion method (Sigma-Aldrich).
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bioRxiv - Cancer Biology 2021Quote: ... the the Cell Trace Far Red Proliferation Kit and the Cell Trace CFSE Cell Proliferation Kit (Invitrogen) was used ...
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bioRxiv - Immunology 2021Quote: Cell cultures RK13 cells were grown in RK13 growth media in adherent cell culture flasks (Nunc EasYFlask) to 80-90% confluency ...
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bioRxiv - Developmental Biology 2022Quote: ... and subjected to single-cell sequencing (scRNAseq) following cell counting with countess II automated cell counter (Invitrogen).
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bioRxiv - Cancer Biology 2022Quote: ... Single-cell suspensions were generated by filtering trypsinized cells through a 40 mm cell strainer (Fisher Scientific). 500-1000 cells per well were seeded into white clear-bottom 96-well plates in quadruplicate ...
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bioRxiv - Immunology 2024Quote: ... Cells were then fixed and permeabilized (FIX & PERM Cell Fixation & Cell Permeabilization Kit, Life Technologies, Oslo, Norway) and stained for CD3 (clone UCHT1 ...
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bioRxiv - Developmental Biology 2023Quote: ... cells were counted using an automatic cell counter (Invitrogen™ Countess™ 3 FL Automated Cell Counter) and diluted in NDiff227 to a final concentration 10 cells/µl ...
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bioRxiv - Microbiology 2023Quote: ... The viability and cell count of the resuspended cells were measured using Countess Automated Cell Counter (Invitrogen). The buffer used for antibody staining and washing is 1X PBS+ 1% FBS ...
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bioRxiv - Neuroscience 2023Quote: ... Whole cell protein extraction was performed by lysing the cell pellet with Cell Lysis buffer (ThermoFisher, FNN0011) supplemented with protease inhibitor cocktail (ThermoFisher ...
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bioRxiv - Immunology 2023Quote: Pleural cavity cells were counted using the Countess Cell Counter (Countess Cell Counter, Thermo Fisher, Massachusetts, USA). Cells were then stained for live/dead (Life Technologies ...
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bioRxiv - Molecular Biology 2024Quote: ... HEK293T cells (1 × 105 cells/cm2) were seeded into 12-well Cell-Culture Treated Multidishes (Thermo Scientific) and incubated overnight ...
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bioRxiv - Biochemistry 2020Quote: ... Lymphocytes cells were grown in cell culture medium (RPMI) (Invitrogen). All cultured media were supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Cancer Biology 2021Quote: Cells were dissociated into single cells with StemPro Accutase (Gibco). Apoptosis assay was conducted using FITC-Annexin V Apoptosis Detection kit (BD Biosciences ...
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bioRxiv - Cell Biology 2020Quote: ... We measured cell size using Countess automated cell counter (Invitrogen). We measured each cell line at least four times and take average of measured cell sizes ...
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bioRxiv - Neuroscience 2022Quote: ... Cell counts were determined using a cell counter (Invitrogen Countess) and Fc block (BD Biosciences 553142 ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell numbers were counted with Countess cell counting chambers (Invitrogen). Statistical analyses were determined by a Mann-Whitney test (ns p>0.05 ...