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Citations for Thermo Fisher :
7451 - 7500 of 10000+ citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: Pleural cavity cells were counted using the Countess Cell Counter (Countess Cell Counter, Thermo Fisher, Massachusetts, USA). Cells were then stained for live/dead (Life Technologies ...
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bioRxiv - Immunology 2024Quote: ... Cells were then fixed and permeabilized (FIX & PERM Cell Fixation & Cell Permeabilization Kit, Life Technologies, Oslo, Norway) and stained for CD3 (clone UCHT1 ...
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bioRxiv - Developmental Biology 2023Quote: ... cells were counted using an automatic cell counter (Invitrogen™ Countess™ 3 FL Automated Cell Counter) and diluted in NDiff227 to a final concentration 10 cells/µl ...
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bioRxiv - Developmental Biology 2022Quote: ... and subjected to single-cell sequencing (scRNAseq) following cell counting with countess II automated cell counter (Invitrogen).
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bioRxiv - Cancer Biology 2022Quote: ... Single-cell suspensions were generated by filtering trypsinized cells through a 40 mm cell strainer (Fisher Scientific). 500-1000 cells per well were seeded into white clear-bottom 96-well plates in quadruplicate ...
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bioRxiv - Molecular Biology 2024Quote: ... HEK293T cells (1 × 105 cells/cm2) were seeded into 12-well Cell-Culture Treated Multidishes (Thermo Scientific) and incubated overnight ...
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bioRxiv - Genomics 2020Quote: Optimal establishment of primary human hepatocytes in tissue culture required a substratum of type 1 collagen (24-well plates; ThermoFisher Scientific #A11428-02) and initial plating at 200,000 cells/well in complete Williams’ E medium containing supraphysiological concentrations of insulin ...
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bioRxiv - Genomics 2020Quote: Small-interfering (si)RNA knockdown of LOC157273 was performed on primary human hepatocytes from donor Hu8200 (genotype G/G at rs4841132) plated into collagen-coated 6-well plates (ThermoFisher Scientific #A11428-01). Cells were incubated in complete Williams’ E medium and transfected with siRNA (50 nM ...
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bioRxiv - Bioengineering 2021Quote: MOLM-13 cell clones cultured on-chip for 72 h were stained for the hematopoietic marker CD45 using a PE-conjugated primary antibody (mouse anti-human CD45-PE, Invitrogen #12-0451-82). Prior to imaging ...
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bioRxiv - Molecular Biology 2020Quote: ... 150 ng of labelled probe was combined with 5 μg of salmon sperm and 10 μg of human Cot1 DNA (Invitrogen, Cat No 15279011). Two volumes of ethanol were added and the probe mix was collected by centrifugation and dried ...
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bioRxiv - Genomics 2021Quote: ... The over-expression construct of human LY6 was created by PCR amplification of cDNA by Phusion® High-Fidelity DNA polymerase (Thermo Fisher Scientific), using the following primers LY-6S-Pac1-S-5’-GGAACGTTAATTAACGCTGAAGTTTGTCTGTGCACTA-3’ ...
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bioRxiv - Cell Biology 2021Quote: ... The iPSCs were cultured on irrMEF with medium for human iPSCs : KnockoutTM-Dulbecco’s modified Eagle’s medium (KO-DMEM: 10829-018, Life Technologies; Thermo Fisher Scientific, MA, USA) supplemented with 20% KnockoutTM-Serum Replacement (KO-SR ...
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bioRxiv - Pathology 2021Quote: ... for 1 week and pepsin (1 mg/ml in 0.1N HCl) was used for antigen retrieval for immunostaining with human COMP (Thermofisher; MA1-20221, 1:100), CHOP (Santa Cruz ...
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bioRxiv - Molecular Biology 2020Quote: ... qRT-PCR was performed using TaqMan gene expression assays (Human-Assay ID Hs00917510_m1; and (Rat-Assay ID Rn01489483_m1; Applied Biosystems, Life Technologies, Carlsbad, CA). Gene expression was quantified by the comparative Ct value ...
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bioRxiv - Microbiology 2020Quote: ... media with 0.05 mg/mL bovine pituitary extract and 5 ng/mL human recombinant epidermal growth factor (Gibco - Thermo Fisher Scientific, MA, USA). All culture conditions contained a 5% antibiotic / antimycotic mixed solution (Nacalai Tesque) ...
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bioRxiv - Neuroscience 2022Quote: ... using a commercial human fetal brain cDNA library containing cDNAs fused to the gal4 activation domain of pEXP-AD502 (ProQuest™, ThermoFisher Scientific, USA), as prey ...
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bioRxiv - Cancer Biology 2022Quote: ... A CRISPR based kinome-wide screening was performed using a lentiviral sgRNA library (LentiArray™ Human Kinase CRISPR Library, Thermo Fisher Scientific, M3775) that knocks out 840 kinase and kinase related genes individually with total number of 3214 sgRNA constructs ...
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bioRxiv - Neuroscience 2021Quote: Lund human mesencephalic (LUHMES) neuronal precursors were grown in T75 flasks in proliferation medium (DMEM/F12 GlutaMAX™ supplement medium (Thermo Fisher Scientific), N2 supplement (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... and the N-terminal peptidase domain of human ACE2 were expressed using the Bac-to-Bac Baculovirus Expression System (Invitrogen, Carlsbad, CA, USA) and purified by Strep-Tactin Sepharose (IBA Lifesciences ...
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bioRxiv - Cancer Biology 2022Quote: ... Panc1 and MiaPaCa2 with RBFOX2 knockdown and non-targeting control were generated using human Affymetrix Clariom D Arrays (Life Technologies, Cat. no. 902922) with the standard input kit run on the Applied Biosystems GeneChip 3000 instrument ...
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bioRxiv - Systems Biology 2020Quote: ... The amount of VEGF contained in the thawed medium was measured using the protocols and reagents from the VEGF Human ELISA Kit (BMS277-2, Thermo Fisher Scientific, USA), and the amounts of PEDF were measured using a Human ELISA Kit (RD191114200R ...
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bioRxiv - Immunology 2021Quote: ... 32,33 were maintained by serial 2-day passaging in human foreskin fibroblast (HFF-1, ATCC® SCRC-1041™, American Tissue Culture Collection) monolayers cultured in DMEM (Thermofisher scientific) with 10% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2021Quote: ... HeLa cells in 35-mm dishes were transfected with 100 pmol of each siRNA in the Stealth siRNA library targeting 154 human nuclear proteins (Invitrogen–Thermo Fisher Scientific) using Lipofectamine RNAiMax (Invitrogen–Thermo Fisher Scientific ...
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bioRxiv - Genetics 2020Quote: ... T cells were activated through the T cell receptor subunit CD3 and the co-stimulator CD28 by the addition of Human T-Activator CD3/CD28 Dynabeads at a 1:2 bead:cell ratio (Life Technologies, cat. no. 111.31D) for 72 hours.
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bioRxiv - Immunology 2020Quote: ... Cells were cultured for 1 week at a density of 106/well in 6 well plates with 50 ng/ml recombinant human macrophage colony stimulating factor (M-CSF) (Life Technologies, Carlsbad, CA), 1x GlutaMAX-I (Life Technologies ...
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bioRxiv - Biochemistry 2020Quote: The cDNA encoding a fusion protein consisting of GST followed by a 3C cleavage site and the human SMAD2 MH2 domain (residues 241–465) was inserted into the MCSI of the pFastBac™ Dual vector (Thermo Fisher Scientific) in the Sal1 and Spe1 restriction sites ...
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bioRxiv - Cancer Biology 2021Quote: Full-length human SETD7 cDNA sequences were amplified from a human mRNA pool generated by RT-PCR using SuperScript II RNase H Reverse Transcriptase (Life Technologies,CA, USA). The SETD7 cDNA was cloned into the EcoRI/BamHI site of a pCDH-CMV-MCS-EF1α -Puro vector (System Biosciences ...
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bioRxiv - Genetics 2021Quote: ... 250 000 cells in 2 ml of medium in a six-well plate were transfected using 12.5 pmol of Silencer Select siRNA against human ADCY9 (Ambion cat # 4390826 ID 1039), Silencer Select siRNA against CETP (Ambion cat 4392420 ID 2933 ...
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bioRxiv - Microbiology 2020Quote: ... Briefly, parasites were maintained in 2% human O+ erythrocytes (Interstate Blood Bank, Memphis, TN) in RPMI-1640 medium (Life Technologies, Grand Island, NY) supplemented with 0.5% Albumax II (Life Technologies) ...
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bioRxiv - Microbiology 2021Quote: ... Cell suspensions prepared by enzyme-free CDS were kept in PBS-5% FCS and incubated on ice for 30 min with rabbit polyclonal anti-human HVEM (R140; 1:500) (Terry-Allison et al., 1998) followed by visualizing HVEM with anti-rabbit AF488 (Life Technologies; 1:200). The size of the epidermal samples mostly allowed only the analysis of nectin-1 expression ...
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bioRxiv - Biochemistry 2022Quote: Reactivity of recombinant S proteins from Wuhan and VOC Omicron as well as of recombinant RBD (Wuhan, Delta and Omicron) to large set (>9000) of human proteins was assessed by using protein microarrays (ProtoArray®, Invitrogen, Thermo Fischer). First microarray chips were blocked by incubation for 1 hour at 4 °C with buffer containing ...
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bioRxiv - Cell Biology 2023Quote: ... Internalization of Alexa Fluor 488-conjugated human α-syn PFFs (1 μM) and epidermal growth factor (EGF; 4 μg/mL; Thermo Fisher Scientific) were assessed similarly ...
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bioRxiv - Neuroscience 2023Quote: Nuclear and cytoplasmic sub-fractionations of human fibroblasts were carried out by using NE-PER™ Nuclear and Cytoplasmic Extraction Reagents (Thermo Fisher Scientific) as per the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ESCs were maintained on Recombinant Human Protein Vitronectin (Thermo Fischer # A14700) coated plates using mESC maintenance media containing Glasgow Minimum Essential Medium (Thermo Fisher Scientific # 11710035), Embryonic Stem Cell-Qualified Fetal Bovine Serum (Thermo Fisher Scientific # 10439001) ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells stably expressing human recombinant LOXL1 were maintained in culture (37°C, 5% CO2) at log-phase growth in advanced DMEM (Thermo Fisher Scientific, 12491015) with 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Immunology 2023Quote: ... Quantitative gene expression for mouse and human genes was performed using TaqMan probes and TaqMan Fast Advanced Master Mix (Applied Biosystems/Thermo Fisher, 4444554) with a QuantStudio 6 Pro (Applied Biosystems) ...
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bioRxiv - Immunology 2023Quote: ... Freshly isolated CD8+ T cells were differentiated in vitro to CTLs by incubation for 48 h with anti-CD3/CD28 coated magnetic beads (Dynabeads Human T-activator CD3/CD28, Gibco, Waltham, Massachusetts, USA) for cell expansion and activation and 50 U/mL of IL-2 (human Interleukin-2 ...
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bioRxiv - Immunology 2023Quote: The IgG antibodies were extracted from a pool of 100 healthy human serum samples using the Melon™ Gel IgG Spin Purification Kit (Thermo Fisher Scientific), with a tandem purification (one after another ...
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bioRxiv - Genomics 2023Quote: ... Cells were rinsed once in staining buffer and incubated with 2 µg/ml anti-human CD326 conjugated with AlexaFluor 700 (ThermoFisher 56-9326-42) at 4C in the dark for 1h ...
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bioRxiv - Microbiology 2023Quote: Isolates from wastewater and isolates provided from collaborators (8 animal/food, 6 environment, 1 human skin, 137 UTI) were grown in tryptic soy broth (TSB; Thermo Scientific; cat. CM0129B) for 24 – 48 hours at 37°C to an OD600 of ∼1 ...
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bioRxiv - Neuroscience 2022Quote: ... HLA-DR Antibody (Monoclonal Rabbit Anti-Human, Citrate Buffer HIER, dilution 1:50 Clone: LN-3, Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA), TMEM119 (Rabbit Anti-Human ...
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bioRxiv - Physiology 2022Quote: Human TRPM6 was either transfected in a bicistronic vector pCINeo/IRES-GFP plasmid or in a pcDNA pDEST53 plasmid (ThermoFisher Scientific, Waltham, MA) (26 ...
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bioRxiv - Molecular Biology 2022Quote: Human adult epidermal keratinocytes were cultured in EpiLife medium supplemented with 10% Human Keratinocyte Growth Supplement (HKGS) and 1% Penicillin and Streptomycin at 37℃ in 5% CO2 (all from ThermoFisher Scientific, Waltham, MA). Calcium concentration was increased to 1.5 mM in the medium to induce cell differentiation ...
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bioRxiv - Bioengineering 2022Quote: Epidermal growth factor receptor (EGFR) protein expression levels on the surface of Gli36-derived EVs were quantified using an EGFR Human ELISA kit (ThermoFisher Scientific, Waltham, MA). EVs were spiked in healthy donor serum at different concentrations ranging from 0 to 1.0E11 particles/mL while maintaining the serum-derived EV concentration at 1.0E9 particles/mL ...
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bioRxiv - Cancer Biology 2022Quote: Expression levels of 754 miRNAs were quantified using the TaqMan Human MicroRNA Array A+B Card Set v3.0 (Applied Biosystems, Foster City, CA).
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bioRxiv - Molecular Biology 2024Quote: ... crude and enriched synaptosomal preparations and surgically resected human brain samples was extracted using TRIzol Plus RNA Purification Kit and Phasemaker Tubes Complete System (Invitrogen, cat. no. A33254). The remaining gDNA was removed using TURBO DNA-free Kit (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... The following siRNAs were used: control siRNA that have no homology to any sequence in the human genome were used as the controls (Thermo Fisher Scientific, #4390846); PFKFB3 siRNAs (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA of human TAF2 was amplified by PCR with gene-specific primers fused to attB recombination sequences for Gateway® cloning (Thermo Fisher Scientific). The amplified sequence was recombined via BP reaction into the pDONR201 donor resulting in pENTR-TAF2 for N-terminal tagging ...
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bioRxiv - Cell Biology 2024Quote: ... 70% confluent EnSC monolayers in 6-well plates were downregulated overnight in 2% DCC-FBS/DMEM/F12 media and transfected with 50nM Stealth siRNA against human GIPC1 or APPL1 (Fisher Scientific, Loughborough, U.K.). For depletion of receptor expression ...
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bioRxiv - Cell Biology 2024Quote: Plasma TPO levels were estimated in plasma samples using Human PracartaPlex Mix&Match 4-Plex Luminex kit (ThermoFisher Scientific, Cat # PPX-04-MX323UK). Assays were performed as per manufacturer’s instructions ...