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Citations for Thermo Fisher :
7101 - 7150 of 10000+ citations for 5 Nitrofluorescein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... as well as the eluate from RNA-ChIP were treated by 5 U of TURBO-DNase (Ambion) 1 h a 37°C prior purification by acidic phenol/chloroform method and ethanol-precipitation with 5µg of Glycoblue (Ambion ...
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bioRxiv - Molecular Biology 2022Quote: ... supernatant were purified by acidic phenol/chloroform method and ethanol-precipitation with 5 µg of Glycoblue (Ambion) as carrier ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were cultured at 37°C and 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM) (Thermo Fisher) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2022Quote: ... heated for 5 minutes at 95C and separated on 4-12% NuPAGE® Bis-Tris gel (Invitrogen) by electrophoresis and subsequently transferred onto Immobilon-P™ PVDF membranes of 0.45 μm pore size (Millipore® ...
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bioRxiv - Molecular Biology 2022Quote: ... WT cells were transfected with 250□Jµl Opti-MEM that contained 5□Jµl Lipofectamine 2000 (Thermo Fisher) and□J2.5µg sgRNA-pX330-mCherry plasmid ...
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bioRxiv - Molecular Biology 2022Quote: ... Peptides were separated across a 2.1 mm x 5 cm C18 column (1.9 µl Hypersil Gold, ThermoFisher) with a linear gradient of 4% - 40% CH3CN and 0.3% formic acid ...
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bioRxiv - Molecular Biology 2022Quote: ... then treated samples with RNase A (5 U/ml) and TURBO DNase (1 U/ml, Thermo fisher) for 30 min ...
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bioRxiv - Molecular Biology 2022Quote: ... and 5% CO2 in a humidified cell culture chamber (Heracell™ VIOS 160i CO2; Thermo Scientific™).
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bioRxiv - Bioengineering 2022Quote: ... 5’ppp-RNA was prepared by IVT in the absence of ATP using MEGAshortscript T7 kit (Thermofisher) and DNA template ...
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bioRxiv - Biophysics 2022Quote: ... Both cell lines were cultured at 37 °C and in 5% CO2 atmosphere in DMEM (Life Technologies) medium containing 10% heat-inactivated FBS (Life Technologies ...
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bioRxiv - Physiology 2022Quote: ... and primer pairs were run in triplicates on a QuantStudio 5 Real-time PCR instrument (Applied Biosystems). Primers were designed using the NCBI/Primer Blast tool (Table 1 ...
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bioRxiv - Neuroscience 2022Quote: ... 5 μg RNA were reverse-transcribed in a reaction containing 1 μl dNTP (10 mmol/l; Invitrogen), 0.5 μg oligo(dT ...
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bioRxiv - Physiology 2022Quote: ... Fresh retinas were minced and incubated in 5 ml DMEM containing 200 U/ml collagenase I (Invitrogen) for 45 mins at 37°C with occasional shaking followed by filtering through a 40 μm nylon mesh ...
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bioRxiv - Cancer Biology 2022Quote: ... 5 μm sections were taken from the FFPE tissue using a microtome (Thermo Scientific HM355S; MX35 blades). For the Chromium Fixed RNA Profiling (scFFPE-seq ...
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bioRxiv - Cell Biology 2022Quote: CHO cells were kept at 37°C and 5% CO2 in OptiMEM™ + Glutamax™ (Life Technologies) supplemented with 10% heat-inactivated endotoxin-low FCS ...
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bioRxiv - Pathology 2024Quote: ... The qPCR reactions were performed in triplicates using a QuantStudio 5 Real- time PCR System (Thermo Fisher) using the following thermocycling conditions ...
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bioRxiv - Physiology 2023Quote: ... Fixation was quenched 1 ml of 1.375 M glycine (Cat# BP381-5, Thermo Fisher Scientific, Waltham, MA) with gentle nutation for 5 mins at room temperature ...
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bioRxiv - Microbiology 2024Quote: Reverse transcription quantitative PCR analysis was performed on QuantStudio 5 Real-Time PCR system (Thermo Scientific, USA) using the primer set from E or RdRp Charité (IDT Coralville ...
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bioRxiv - Microbiology 2024Quote: ... CRL-3216) were cultured at 37⁰C in 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM, Gibco) supplemented with 10% foetal calf serum (FCS ...
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bioRxiv - Genetics 2024Quote: ... Slides were covered by mounting medium with nuclear counterstain DAPI (Thermofisher, catalog number D1306, 5 ng/ml). Immunohistochemistry for zebrafish was performed as described20 ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative PCR analyses were performed with 5 µl of cDNA using SYBR© Green fluorophore (Applied Biosystems), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... cells were harvested using pre-mixed Earle’s Balanced Salt Solution (EBSS) with 5 mM EDTA (Life Technologies) and centrifuged at 3000 rpm (10 min ...
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bioRxiv - Cell Biology 2024Quote: ... All cells were cultured in a 95% air/5% CO2 Thermo Forma incubator (Thermo Scientific, Waltham, MA) at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... 5% CO2 and 95% humidity in D10 medium: Dulbecco’s modified Eagle medium (DMEM) with high glucose (Gibco) supplemented with 10% v/v fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2024Quote: ... containing 100 mg/ml of X-gal (5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside) (Thermofisher) to confirm CFU/ml counts.
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bioRxiv - Microbiology 2024Quote: ... Membranes were washed 3 x 5 min with TBST and developed using chemiluminescent substrate (ThermoFisher SuperSignal West) and imaged using a ChemiDoc (Bio-Rad) ...
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bioRxiv - Molecular Biology 2024Quote: ... The digestion was quenched with 5% formic acid and desalted either using C18 tips (Thermo Fisher 87782) or equal mix of Sera-Mag Carboxylate SpeedBeads (Cytiva 65152105050250 & 45152105050250)100 ...
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bioRxiv - Immunology 2024Quote: ... we lysed 5 × 106 pelleted neutrophils with 300 µl of TRIzol® Reagent (Life Technologies, Carlsbad, CA) and stored at -80 °C ...
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bioRxiv - Neuroscience 2024Quote: HEK293T cells (ECACC, #12022001) were cultured at 37°C under 5% CO2 in DMEM (Gibco, #11995-065) supplemented with 10% FBS (fetal bovine serum) ...
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bioRxiv - Neuroscience 2024Quote: The tissue sample was milled and imaged using a Helios 5 CX FIB-SEM (Thermo Fisher Scientific). After mounting onto the SEM stub using silver paste ...
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bioRxiv - Molecular Biology 2024Quote: ... paraffin embedded and cut into 5 μm sections using an automatic microtome (Microm HM355, Thermo Fisher Scientific). Prior to staining ...
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bioRxiv - Microbiology 2024Quote: ... at a flow rate of 5 µl/min and then passed through the analytical column (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: Dissociated neurons were spun down at 1000 rpm for 5 mins and resuspended in Neurobasal medium (Gibco) supplemented with 2% B-27 (Gibco) ...
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bioRxiv - Microbiology 2023Quote: ... Tomographic tilt series between −60° and +60° were collected using TEM Tomography 5 software (Thermo Fisher Scientific) in a dose-symmetric scheme 67 with a 3-degree angular increment starting at 0 degree tilt angle ...
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bioRxiv - Microbiology 2024Quote: ... cultures were treated twice with 10 ml of 5% D-sorbitol (Thermo Fisher Scientific, Ward Hill, MA) 46 h apart ...
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bioRxiv - Cell Biology 2024Quote: ... Neurons were maintained at 37°C in a 5% CO2 atmosphere in Neurobasal A medium (Gibco #10888022) supplemented with B27 (Gibco #11530536 ...
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bioRxiv - Cell Biology 2024Quote: ... plates were washed thrice for 5 minutes with 200 µL 1X PBS-T (ThermoFisher Scientific, Cat# 28352), and then blocked overnight at 4°C with 200 µL of 2% BSA in PBS-T to prevent non-specific binding ...
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bioRxiv - Cell Biology 2024Quote: Vacuolar pH alterations were detected using the 5-(and-6)-carboxy-2′,7′-dichlorofluorescein diacetate (CDCFDA, Invitrogen) probe ...
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bioRxiv - Immunology 2023Quote: ... washed 5 times with PBS-T and incubated with 1x TMB ELISA substrate solution (Thermo Fisher Scientific). The reaction was stopped with 2 N H2SO4 ...
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bioRxiv - Neuroscience 2023Quote: ... hiPS cells were cultured on plates coated with vitronectin (5 μg ml−1; Thermo Fisher Scientific, A14700) in Essential 8 medium ...
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bioRxiv - Microbiology 2024Quote: ... THP-1 cells were cultured at 37°C under a 5% CO2 atmosphere in RPMI1640 + Glutamax (Gibco), 10% Fetal Calf Serum (FCS ...
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bioRxiv - Cell Biology 2024Quote: ... mRNA was transcribed from a PCR template using the 5×m Message mMachine T7 ULTRA kit (Invitrogen) according to the manufacturer’s instructions as previously reported76.
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bioRxiv - Cell Biology 2024Quote: ... TwinStrep-tagged proteins were concentrated using 30K MWCO concentrators to ∼ 5 mg/ml (Thermo Scientific™, USA). Protein was stored at −80°C.
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bioRxiv - Cell Biology 2024Quote: ... Human embryonic lung fibroblasts (MRC-5, European Collection of Cell Cultures) were maintained in DMEM (Gibco/BRL) supplemented with 10% (v/v ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then incubated with 5 μM of the fluorescent lipid probe VybrantTM DiD (Thermo Fisher Scientific) or 10 μM of the intracellular content probe Green CMFDA cell tracker or Deep Red cell tracker (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... 30 minutes prior to fixing cells were incubated with 1 µM 5-Ethynyl Uridine (EU) (Invitrogen #E10345), except for the unlabeled control ...
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bioRxiv - Developmental Biology 2024Quote: ... was prepared and added to 426.5 μL 2:1 (vol:vol) 0.1x Tris (Fisher Scientific, cat.# BP152-5)-ethylenediaminetetraacetic acid (EDTA ...
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bioRxiv - Evolutionary Biology 2024Quote: HEK293T cells were grown at 37°C with 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM, Gibco) supplemented with 10% (v/v ...
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bioRxiv - Genomics 2024Quote: ... Cells were cultured at 37 °C with 5% CO2 and passaged using Versene solution (#15040066, Thermo Fisher). HAP1 cells were cultured in Iscove’s Modified Dulbecco’s Medium (IMDM ...
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bioRxiv - Immunology 2024Quote: ... Treg cells were stained with 5 uM ViaFluor 405 (Biotum) or 1uM CellTrace Far Red (Molecular Probes) dyes according to the manufacturer’s protocol ...