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Citations for Thermo Fisher :
7151 - 7200 of 10000+ citations for 5 Nitrofluorescein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: Vacuolar pH alterations were detected using the 5-(and-6)-carboxy-2′,7′-dichlorofluorescein diacetate (CDCFDA, Invitrogen) probe ...
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bioRxiv - Cell Biology 2024Quote: ... plates were washed thrice for 5 minutes with 200 µL 1X PBS-T (ThermoFisher Scientific, Cat# 28352), and then blocked overnight at 4°C with 200 µL of 2% BSA in PBS-T to prevent non-specific binding ...
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bioRxiv - Immunology 2024Quote: ... Treg cells were stained with 5 uM ViaFluor 405 (Biotum) or 1uM CellTrace Far Red (Molecular Probes) dyes according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... rProprotein convertase subtilisin/kexin type 5 (PCSK5) was expressed in the ExpiCHO expression system produced by Gibco™ ...
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bioRxiv - Microbiology 2024Quote: HEK293T cells were grown at 37°C with 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco) supplemented with 10% fetal bovine serum (Atlanta Biologicals ...
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bioRxiv - Molecular Biology 2024Quote: ... paraffin embedded and cut into 5 μm sections using an automatic microtome (Microm HM355, Thermo Fisher Scientific). Prior to staining ...
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bioRxiv - Microbiology 2023Quote: ... Tomographic tilt series between −60° and +60° were collected using TEM Tomography 5 software (Thermo Fisher Scientific) in a dose-symmetric scheme 67 with a 3-degree angular increment starting at 0 degree tilt angle ...
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bioRxiv - Microbiology 2024Quote: ... cultures were treated twice with 10 ml of 5% D-sorbitol (Thermo Fisher Scientific, Ward Hill, MA) 46 h apart ...
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bioRxiv - Microbiology 2024Quote: ... THP-1 cells were cultured at 37°C under a 5% CO2 atmosphere in RPMI1640 + Glutamax (Gibco), 10% Fetal Calf Serum (FCS ...
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bioRxiv - Cell Biology 2024Quote: ... mRNA was transcribed from a PCR template using the 5×m Message mMachine T7 ULTRA kit (Invitrogen) according to the manufacturer’s instructions as previously reported76.
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bioRxiv - Bioengineering 2023Quote: ... The cells were subsequently detached by incubating for 5 min in 0.05% trypsin (Gibco, Cat. No. 25300054) at 37°C ...
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bioRxiv - Bioengineering 2023Quote: ... and the fluorescence signals were traced by the quantitative realtime PCR QuantStudio 5 (Thermo Fisher Scientific, USA) with λex = 495 nm and λem = 520 nm ...
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bioRxiv - Cancer Biology 2023Quote: Pancreas from mice aged 10 weeks was harvested and rinsed twice in 5 mL cold HBSS (Gibco). Tissue was minced with sterile scissors into 1-5 mm sized pieces then centrifuged for 2 minutes at 300 g and 4°C ...
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bioRxiv - Evolutionary Biology 2023Quote: We conducted all experiments using SM/5 media (Loomis and Sussman 1966) (2 g glucose (Fisher Scientific), 2 g BactoPeptone (Oxoid) ...
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bioRxiv - Cell Biology 2023Quote: ... Fixed and permeabilized cells were washed twice in PBS and blocked in 5% normal goal serum (Gibco)/1% BSA for 30 min at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... 5 mM EGTA) then simultaneously fixed and permeabilized in 1.8% formaldehyde (methanol-free, Thermo Fisher Scientific 28906) and 0.5% Triton-X100 in CSK for 10 min ...
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bioRxiv - Immunology 2023Quote: ... containing final percentages/concentrations as follows: 5% heat-inactivated newborn calf serum (NCS; Gibco catalog number 26010074), 2% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2023Quote: Pregnant dams were injected intraperitoneally at e12.5 with a solution containing 5-Ethynyl-2’-deoxyuridine (EdU, ThermoFisher) and embryos were collected 2h after ...
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bioRxiv - Biochemistry 2022Quote: ... pH 7.6) containing 5% milk followed by incubation with mouse Engelbreth-Holm-Swarm (EHS) laminin (ThermoFisher, 23017015) overnight at a concentration of 7.5 nM at 4 °C in LBB containing 3% bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2023Quote: ... temperature-dependent protein unfolding profiles were measured using the QuantStudio™ 5 realtime PCR machine (Thermo Fisher). Excitation and emission filters were set to 465 nm and 590 nm ...
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bioRxiv - Cell Biology 2023Quote: ... The slides were washed once with PBS for 5 minutes before coverslips (12-545-100, Thermo Fisher) were mounted with Gelvatol mounting media (81365 ...
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bioRxiv - Cell Biology 2023Quote: ... 0.1% sodium dodecyl sulfate and 5 mM ethylenediaminetetraacetic acid with protease and phosphatase inhibitors (Thermo Fisher Scientific). Cell lysates were centrifuged at 500 g for 30 minutes at 4°C to remove cellular debris ...
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bioRxiv - Cancer Biology 2022Quote: ... minced with scalpels and enzymatically digested in RPMI supplemented with collagenase I (Life Technologies, 5 mg/ml) and DNase I (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... at 37 °C and 5% CO2 and supplemented with 1% penicillin-streptomycin (Life Technologies Australia Pty. Ltd). MDCK-I cells were grown in Modified Eagle Medium (MEM ...
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bioRxiv - Microbiology 2022Quote: ... 5 μm (bead size) column) coupled to a Thermo Orbitrap Q-Exactive mass spectrometer (Thermo Fisher Scientific). 10 μl of the sample maintained on a 5° C auto-sampler was injected on a column that was maintained at 30 C ...
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bioRxiv - Microbiology 2023Quote: ... Hybridization was performed using 5’-labeled oligonucleotides or riboprobe labelled with P32-UTP using Ultra-Hyb (Ambion) hybridization buffer at 42°C for a minimum of 4 hours ...
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bioRxiv - Immunology 2022Quote: ... 1 μl cDNA was added to 5 μl of Power SYBR Green Master Mix (Life Technologies Ltd), 0.25 - 2 μl of 10 mM forward and reverse oligonucleotide primer mix concentration optimized previously (Table 1) ...
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bioRxiv - Molecular Biology 2023Quote: 5 μg of total RNA (quantified by Nanodrop) was digested with TURBO DNase (Thermo Fisher Scientific AM2238) in a 20 μL reaction following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... Cultures were maintained at 37 °C in 5% CO2 in growth media (Neurobasal-A (Life technologies 1088802) supplemented with B27 (Life technologies 17504044) ...
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bioRxiv - Molecular Biology 2023Quote: Cells were cultured at 37 °C with 5% CO2 in high-glucose DMEM medium (Life Technologies, 31966047) supplemented with 10% (vol/vol ...
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bioRxiv - Neuroscience 2023Quote: ... The BV-2 cells were cultured in DMEM supplemented with 5% heat-inactivated fetal bovine serum (Gibco) and 1% penicillin-streptomycin ...
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bioRxiv - Biochemistry 2023Quote: HeLa cells were cultured at 37 °C under 5 % CO2 in Dulbecco’s modified Eagle’s medium (DMEM, Invitrogen), supplemented with 10 % fetal bovine serum ...
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bioRxiv - Systems Biology 2022Quote: ... and 5 % DMSO was added to the optimized PCR master mix containing DreamTaq DNA polymerase (Thermo scientific), reaction buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... the diluted cDNA was mixed with 5 µL PowerUp SYBR Green master mix (Applied Biosystems, cat#A25743), and the mix of primers (forward+reverse ...
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bioRxiv - Cell Biology 2023Quote: ... At 3 hours post infection (hpi) cells were seeded on 5 mm round glass coverslips (Thermo Scientific) and mounted on a sample holder specially designed for LLSM ...
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bioRxiv - Microbiology 2023Quote: Human 293F cells were maintained at 37°C with 5% CO2 in FreeStyle 293 Expression Medium (ThermoFisher) supplemented with penicillin and streptomycin ...
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bioRxiv - Biophysics 2023Quote: ... Membranes were blocked with 5% (w/v) bovine serum albumin in phosphate-buffered saline (Thermo Fisher, Oxoid) with 0.1% (v/v ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We incubated slides for 5 min in DAPI (1mg/ml) before mounting in SlowFadeTM Gold (Invitrogen S36936).
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5% fetal bovine serum (FBS) and 1% antibiotics (penicillin G/streptomycin; Gibco, Waltham, MA, USA) in a humidified atmosphere containing 5% CO 2 at 37 °C.
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bioRxiv - Neuroscience 2023Quote: ... Primary mouse astrocytes (passage 0 - 5) were maintained in GeltrexTM-coated T75 culture flask (Thermo Fisher Scientific) and re-plated at 5.7 x 104 cells/cm2 to 1 x 105 cells/cm2 3-5 days prior to seeding hypothalamic progenitors for the co-culture experiments including immunocytochemistry ...
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bioRxiv - Microbiology 2023Quote: ... Samples were boiled for 5 minutes then run on 12% Bolt SDS-PAGE Bis/Tris gels (Invitrogen). Contents of SDS-PAGE gels were transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Immunology 2023Quote: ... at 95°C for 5 min and separated on a 12% Bis-Tris gel (NuPAGE, Thermo Scientific) for 30 min at 170 V ...
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bioRxiv - Cell Biology 2023Quote: ... HoxB8-macrophages CTL and TKO-ERM were differentially labeled with 5 µM Green CellTracker CMFDA (Invitrogen #C7025), or Red CellTracker CMPTX (Invitrogen #C34552 ...
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bioRxiv - Cell Biology 2023Quote: ... and the sample was washed with 1X PBS for 2 x 5 min using a Pipetman (Gibco), then blocked using the blocking solution for 1 h ...
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bioRxiv - Biochemistry 2023Quote: ... 5 mg of microsome fraction was mixed with loading dye (NuPAGE™, Thermo Fisher Scientific, MA, USA) and a reducing agent (NuPAGE™ Sample Reducing Agent ...
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bioRxiv - Cancer Biology 2023Quote: ... p16 and p21 mRNA was determined by quantitative reverse-transcription PCR (QuantStudioTM 5 Real-Time, Applied Biosystems) normalized to β-actin with 100ng/ml doxorubicin-treated cells as positive controls ...
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bioRxiv - Cancer Biology 2023Quote: ... Pancreatic tissues were incubated for 24 hours at 4°C in ≥5 volumes of RNAlater solution (ThermoFisher) to preserve RNA integrity ...
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bioRxiv - Cell Biology 2023Quote: ... at 150 × 106 cells/dish for 2 h at 37°C/5% CO2 in RPMI-1640 (Gibco) to separate adherent cells ...
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bioRxiv - Immunology 2023Quote: ... aspiration of supernatant and resuspension of the pellet in 5× Lane Marker Reducing Sample Buffer (Thermo Fisher). Samples were then subjected to SDS-PAGE followed by immunoblot analysis with the indicated antibodies.
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bioRxiv - Neuroscience 2023Quote: ... 5’-CAGAGTCGATCAGTCTGCATATCTCCA-3’) with the dilution protocol of the Phusion Human Specimen Direct PCR Kit (Thermo Scientific). The PCR product was sequenced directly with a nested sequencing primer (5’-GGTGCTCTCCCGGGTACACAA-3’) ...