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Citations for Thermo Fisher :
6951 - 7000 of 10000+ citations for 5 Nitrofluorescein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... RNA (5 ng/µL) was reverse-transcribed using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems). 1 µL of cDNA was used as template in a 10 µL qRT-PCR reaction performed with Power SYBR reagent (Applied Biosystems) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Embedded samples were sectioned into 5 µm slices using a MICRO HM355 (Thermo Fisher Scientific, Walldorf, Germany) instrument ...
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bioRxiv - Molecular Biology 2020Quote: ... qPCR was performed using 1 μL of 5 μM Powerup SYBR green PCR Master Mix (Applied Biosystems), 2 μL of dH2O and 2 μL of cDNA sample in a 10 μL reaction ...
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bioRxiv - Plant Biology 2019Quote: ... and 5 μL of Power SYBR® green RNA-to-CT™ 1-step kit (Applied Biosystems) that has limits of detection as low as 2 copies of the target gene ...
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bioRxiv - Cancer Biology 2020Quote: ... Tumor cells were stained with CellTracker™ Green CMFDA (5-chloromethylfluorescein diacetate) Dye (1 μM, #C7025, Invitrogen) and seeded (5×104/well ...
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bioRxiv - Physiology 2019Quote: ... RT-qPCR was performed on a QuantStudio 5 Real-Time PCR System (Thermo Fisher Scientific, Carlsbad, California), with Fast SYBR Green master mix (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2019Quote: ... The sample was loaded onto the trapping column (Thermo Scientific, PepMap100, C18, 300 μm X 5 mm), using partial loop injection ...
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bioRxiv - Biochemistry 2019Quote: ... The sample was loaded onto the trapping column (Thermo Scientific, PepMap100, C18, 300 μm x 5 mm), using partial loop injection ...
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bioRxiv - Genomics 2020Quote: ... The resulting hairpin molecules were bound on 5 μg of Dynabeads™ MyOne™ Streptavidin T1 (Invitrogen) in PB for 60 min ...
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bioRxiv - Molecular Biology 2020Quote: ... and heated for 5-10 min at 95°C in LDS-sample loading buffer (Life Technologies, NP0008) containing 50 mM dithiothreitol (Amersham Biosciences ...
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bioRxiv - Systems Biology 2020Quote: ... the peptides were dissolved with 0.1% (v/v) TFA and 5% (v/v) acetonitrile (Thermo Fisher Scientific) and subjected to desalting using StageTips with SDB-XC Empore disk membranes (SDB-XC StageTip ...
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bioRxiv - Neuroscience 2020Quote: ... 5 μg of total RNA was reverse transcribed using oligo dT and SuperScript Reverse Transcriptase III (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... containing a 5′-CACC-3′ sequence required for directional TOPO cloning in pENTR/D-TOPO (Invitrogen, USA), and a reverse primer [“Tgd057 R HA stop” 5’-TTACGCGTAGTCCGGGACGTCGTACGGGTACTCGACCTCAATGTTGTATTC-3’] ...
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bioRxiv - Plant Biology 2019Quote: ... total RNA was extracted from phenotypic leaf tissue collected 5 days after treatment using Trizol reagent (Invitrogen) following manufacturers protocol ...
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bioRxiv - Neuroscience 2019Quote: ... 5 μg of PSD-95 antibody was coupled to 1 mg of Dynabeads (Life Technologies, CA, USA) according to the antibody coupling kit protocol (#14311D) ...
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bioRxiv - Cell Biology 2020Quote: ... Quantitative PCR analyses were performed with 5 µl of cDNA using SYBR© Green fluorophore (Applied Biosystems), following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... and applied to a gravity column with 5 mL of HisPur Ni-NTA resin (Thermo Fisher Scientific) prepared in equilibration buffer (40mM Tris [pH 7.4]) ...
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bioRxiv - Molecular Biology 2020Quote: The newly synthesized RNA in OSC cells was labeled with 5-ethynyl-uridine (EU) (Life Technologies, #E10345) as described (61) ...
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bioRxiv - Physiology 2021Quote: ... HRP-linked anti-rabbit IgG (1:10000 in 5% BSA for WB. NP40 (Thermal Fisher Scientific, 28324), Triton™ X-100 (T-9284) ...
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bioRxiv - Neuroscience 2020Quote: ... and kept on ice until sorting and incubated with 5 μg/ml propidium iodide (PI; Life Technologies).
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bioRxiv - Developmental Biology 2020Quote: ... The supernatant was incubated with anti-GFP antibody (5 μg/ml) prebound to Dynabeads Protein A (Invitrogen) for 2h with end-over-end rotation to capture GFP-fused L10a subunit of the 60S ribosome ...
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bioRxiv - Molecular Biology 2021Quote: ... The fragmented chromatin was then pre-cleared with 5 μl of Dynabeads Protein A/Protein G (ThermoFisher) and immunoprecipitated overnight at 4 °C with the pre-prepared antibody-beads complex ...
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bioRxiv - Plant Biology 2021Quote: ... a standard PCR (RT-PCR) was then performed using DreamTaq DNA polymerase (5 U/μL) (Thermo Scientific). VIGS BAK1 silencing was confirmed as previously described (Chaparro-Garcia et al. ...
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bioRxiv - Physiology 2021Quote: ... Membranes were blocked with 5% skim milk and incubated with antibodies against NRTN (Thermo Scientific PA5-18769), SV2a (Abcam ab32942) ...
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bioRxiv - Neuroscience 2019Quote: ... the fibroblasts were plated at 5×104 – 1×105 onto irradiated mouse embryonic fibroblasts (Gibco, #S1520‒100). On day 8 ...
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bioRxiv - Biophysics 2019Quote: Cells plated on dishes were labelled with 1:200 dilution of 5 mM Laurdan (D250, Molecular Probes), incubated at 37 °C for 30 min ...
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bioRxiv - Molecular Biology 2021Quote: ... HeLa cells were transfected using 100 pmol of siRNA with 5 μL of Lipofectamine 2000 (Invitrogen, 11668019) for 72 h 76 ...
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bioRxiv - Microbiology 2020Quote: ... Each reaction used 5 µl of 2X SYBR Green PCR Master Mix (Applied Biosystems, Carlsbad, CA, USA), 4 µl of template DNA ...
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bioRxiv - Microbiology 2021Quote: Human 293F cells were maintained at 37° Celsius with 5% CO2 in FreeStyle 293 Expression Medium (ThermoFisher) supplemented with penicillin and streptomycin ...
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bioRxiv - Microbiology 2020Quote: ... 5 x 106 S2 cells were seeded in 2 mL Schneider’s Drosophila media (Thermo Fisher, Waltham, Massachusetts) supplemented with 10% heat-inactivated fetal bovine serum in 9.6 cm2 wells of a tissue culture plate and incubated at 26 °C ...
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bioRxiv - Microbiology 2021Quote: ... The RNA was labelled at its 5′ end with [32P]-γ-ATP and T4 Polynucleotide Kinase (Ambion) in a standard reaction ...
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bioRxiv - Microbiology 2021Quote: Human 293F cells were maintained at 37° Celsius with 5% CO2 in FreeStyle 293 Expression Medium (ThermoFisher) supplemented with penicillin and streptomycin ...
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bioRxiv - Microbiology 2021Quote: ... for 3 hours at 37 °C before transforming 5 μL into TOP 10 chemically-competent bacteria (Invitrogen). Mutations were confirmed in selected clones by DNA sequencing ...
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bioRxiv - Developmental Biology 2020Quote: ... and 5 µL of 2X Luminaris Color HiGreen qPCR Master Mix Low Rox (K0973, Thermo Fisher Scientific). Thermocycling conditions were 50 °C during 2 min ...
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bioRxiv - Cell Biology 2020Quote: ... cells were washed twice with Phosphate-Buffed Saline (PBS) after which 5 μM calcein-AM (Thermofisher, C1430) in PBS was added ...
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bioRxiv - Neuroscience 2019Quote: ... followed by overnight 4°C incubation with rabbit polyclonal antibodies against claudin-5 (1:100; Life Technologies) and ZO-1 (1:200 ...
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bioRxiv - Pathology 2021Quote: ... These cells were grown in DMEM/F12 (D6421) supplemented with 5% FBS (GIBCO/ThermoFisher, Grand Island, NY) and maintained at 37°C in 5% CO2 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Allele sizes for the genotyping and assays were scored with GeneMapper 5 (Thermo Fisher Scientific, Waltham (MA), USA).
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bioRxiv - Systems Biology 2020Quote: ... RNA was radioactively 5' end-labeled using 1 U μl−1 T4 polynucleotide kinase (Thermo Fisher Scientific) and 0.5 μCi μl−1 32P-γ-ATP (PerkinElmer ...
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bioRxiv - Synthetic Biology 2020Quote: 0.63 mmole (1 equivalent) of the nucleoside-5’-monophosphate free acid (Santa Cruz Biotechnology or ACROS Organics) and 5 equivalents of 2-aminoimidazole hydrochloride (Combi-Blocks ...
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bioRxiv - Molecular Biology 2021Quote: ... Pellets were suspended in 500 μL washing solution containing 8mL dimethyl sulfoxide (Fisher Scientific, 67-68-5), 0.2 g magnesium chloride hexahydrate (MP Biomedicals ...
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bioRxiv - Genomics 2021Quote: ... One-step reverse transcription and real-time PCR was performed with a Quantstudio 5 (Thermo Fisher Scientific) using Power SYBR Green RNA-to-CT 1-Step Kit (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... Sorted memory CD4+ T cells were labelled with 5-(and 6)-carboxyfluorescein diacetate succinimidyl ester (CFSE, ThermoFisher) and cultured at a ratio of 2:1 with irradiated autologous monocytes untreated or pulsed for 3 h with recombinant SARS-CoV-2 Spike protein (2.5 μg/ml) ...
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bioRxiv - Physiology 2020Quote: ... The membrane was probed with primary antibodies for α-Tubulin (ThermoFisher 322588, clone B-5-1-2), acetylated (Sigma T7451 ...
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bioRxiv - Immunology 2020Quote: Cells were harvested from the peritoneum by lavage with 5 ml cold PBS + EDTA (2 mM, Gibco). After washing ...
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bioRxiv - Immunology 2021Quote: ... qPCR was performed using 5 μL Powerup SYBR green PCR Master Mix (Applied Biosystems, Foster City, CA), 2 μL of dH2O and 2 μL of cDNA sample in a 10 μL reaction ...
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bioRxiv - Evolutionary Biology 2019Quote: ... we first extracted RNAs from 30 whole 5-day-old flies using the Trizol method (GIBCO BRL) and RNase-free DNase treatment to avoid contamination by genomic DNA (BOGART AND ANDREWS 2006) ...
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bioRxiv - Microbiology 2019Quote: Primary human foreskin fibroblasts were grown for 5 passages in DMEM lacking lysine and arginine (Thermo Scientific) supplemented with 10 % dialysed FCS (Gibco) ...
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bioRxiv - Microbiology 2019Quote: ... 5 µL was injected in a nanoRSLC-Q Exactive PLUS (RSLC Ultimate 3000, Thermo Scientific, MA, USA). Phosphopeptides were loaded onto a µ-precolumn (Acclaim PepMap 100 C18 ...
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bioRxiv - Cell Biology 2019Quote: ... Both cell lines were maintained at 37°C and 5% CO2 in DMEM medium (Gibco #31885-023) containing 10% fetal calf serum (Life Technologies #10270) ...