Labshake search
Citations for Thermo Fisher :
7351 - 7400 of 10000+ citations for 5 Nitrofluorescein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2020Quote: ... WM278 and SKMEL19 cells were maintained at 37 °C with 5% CO2 in RPMI1640 (Thermo Fisher, #22400-089) supplemented with 10% FBS ...
-
bioRxiv - Cell Biology 2020Quote: ... were cultured as above with the addition of 50 μg/mL zeocin and 5 μg/mL blasticidin (Invitrogen) to the culture medium weekly to ensure maintenance of the tet-repressor ...
-
bioRxiv - Cell Biology 2020Quote: Raji cells (ATCC) were cultivated at 37°C and 5% CO2 in RPMI 1640 (Gibco, Thermo Fisher, USA) supplemented with 8% FCS (Lot BS225160.5 ...
-
bioRxiv - Cell Biology 2020Quote: ... were blocked (5% nonfat milk) prior to incubation with primary antibodies diluted in Superblock blocking reagent (Thermo Scientific). Binding was detected by incubation with HRP coupled secondary antibodies (1:10000 ...
-
bioRxiv - Immunology 2021Quote: 5 × 104 isolated PMNs were seeded and incubated for 10min with 5µM Sytox Green dye (Invitrogen Life Technologies) for assessment of NETs with an Axiovert fluorescence microscope coupled to a Zeiss AxioCam colour CCD camera (Carl Zeiss ...
-
bioRxiv - Immunology 2021Quote: 5 × 104 isolated PMNs were seeded and incubated for 10min with 5µM Sytox Green dye (Invitrogen Life Technologies) for assessment of NETs with an Axiovert fluorescence microscope coupled to a Zeiss AxioCam colour CCD camera (Carl Zeiss ...
-
bioRxiv - Immunology 2021Quote: ... RT-PCR was performed on QuantStudio™ 3 and 5 Real-Time PCR System (Thermo Fisher Scientific, USA) using KAPA SYBR®Fast qPCR Master Mix (Kapa Biosystems).
-
bioRxiv - Immunology 2021Quote: ... with 0.8 μM of forward and reverse primers on a QuantStudio 5 Real-Time PCR System (Applied Biosystems) with the following cycling conditions ...
-
bioRxiv - Genetics 2021Quote: ... and Tvrm323 mice (n = 5) at one month of age by homogenizing posterior eyecups in TRIzol (Thermo Fisher) using a gentleMACS dissociator (Milteny Biotec) ...
-
bioRxiv - Microbiology 2021Quote: ... which was boiled in the SDS loading buffer with 5% β-mercaptoethanol (Cat. #60-24-2, Acros Organics). The denatured protein samples were separated by Novex™ WedgeWell™ 4-20% SDS-PAGE Tris-Glycine gel and transferred to PVDF membrane (iBlot™ 2 Transfer Stacks ...
-
bioRxiv - Genomics 2020Quote: ... Here, 40 μL pre-mix (5 μL 10x NEBuffer 2.1, 2 μL 100 mM ATP (Thermo Fisher, R0441), 3 μL 100 mM DTT ...
-
bioRxiv - Molecular Biology 2020Quote: ... BV2 were cultured at 37°C/5% CO2 in growth medium (Dulbecco’s modified Eagle’s medium (D-MEM, Gibco), supplemented with 10% FBS and 1% P/S ...
-
bioRxiv - Neuroscience 2021Quote: ... 5% CO2 and treated with 25 μg/ml transferrin coupled to Alexa Fluor 647 (Tf-647, Life technologies) in NBA medium for 20 min at 37°C ...
-
bioRxiv - Molecular Biology 2021Quote: ... for 30 min in dark at ambient temperature and digested by 5 μg/μL trypsin (Thermo Fisher Scientific) overnight at 37 °C with 600-rpm agitation ...
-
bioRxiv - Molecular Biology 2021Quote: ... 5pmol of primer (5µl of 5 µM stocks) were end-labeled with 10 units of USB Optikinase (Affymetrix), and 7 µl of 6,000Cu/mmol ATP-ɣ32P (Perkin-Elmer ...
-
bioRxiv - Molecular Biology 2021Quote: ... V5-APEX2 alone or IPMK-V5-APEX2 were cloned into the pcDNA™5/FRT/TO plasmid (Invitrogen). Flp-In™ T-REx™-293 (Invitrogen ...
-
bioRxiv - Developmental Biology 2021Quote: ... These vectors were co-transfected into GATA3 reporter cells (G3KI#5-2) using the Lipofectamine Stem reagent (Invitrogen). Two days after transfection ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... rosetta culture (strain SrEpac, ∼106 cells/mL) was filtered through a 5 µm syringe-top filter (Fisher Scientific SLSV025LS ...
-
bioRxiv - Developmental Biology 2021Quote: ... except that animals were permeabilized with 5 µg/ml Proteinase-K for 90 seconds (ThermoFisher Scientific, cat# AM2548), and hybridized with 1ng/µl of RNA probe for a minimum of 48 hours at 62°C.
-
bioRxiv - Cancer Biology 2022Quote: ... Cells were washed 3 x 5 min and incubated for 10 min with DAPI (1/2000, Invitrogen #D1306) diluted in PBT + 0.5% BSA ...
-
bioRxiv - Molecular Biology 2022Quote: ... according to the manufacturer’s instructions in optical plates on a QuantStudio 5 Real-Time PCR system (ThermoFisher Scientific) equipped with 384-well block ...
-
bioRxiv - Synthetic Biology 2022Quote: ... a reaction was prepared by mixing 5 μL of Maxima SYBR green qPCR master mix (Thermo Fisher Scientific), 2 μL of cDNA and 0.5 μL of 2 μM forward and reverse sfGFP qPCR primers ...
-
bioRxiv - Molecular Biology 2022Quote: ... 5’ RACE was carried out as described by the manufacturer’s instructions using purified dsRNAs (Thermo Fisher catalogue #18374058). Amplification of the 5’ ends of the dsRNA using RACE used the specific primers GSP1 ...
-
bioRxiv - Microbiology 2022Quote: ... 293T/ACE2 cells were cultured at 37 °C with 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM, WAKO) containing 10% fetal bovine serum (Gibco) and penicillin/streptomycin (100 U/ml ...
-
bioRxiv - Cell Biology 2022Quote: ... 750,000 cells per well in a 6-well plate were transfected using Lipofectamine 2000 (5 μL; Thermo Fisher) as needed ...
-
bioRxiv - Cancer Biology 2022Quote: ... for 5 minutes followed by a wash with chilled PBS and blocked with 3% goat serum (Thermo Scientific) for 1h at room temperature ...
-
bioRxiv - Cancer Biology 2022Quote: Transformed MEF (5. 105 cells) were resuspended in 100 μl of RPMI medium (Thermo Fisher Scientific; Waltham, MA) and injected subcutaneously into the dorsal area of 4-6 week-old immuno-deficient athymic female mice (Charles River ...
-
bioRxiv - Microbiology 2022Quote: ... the media was replaced with selection media containing 1 µg/mL puromycin or 5 µg/mL blasticidin (ThermoFisher) and cells were selected for 48 h before proceeding with experiments.
-
bioRxiv - Biochemistry 2022Quote: Cells were cultured at 37°C in 5% CO2 atmosphere in Dulbecco’s modified Eagle medium (DMEM, Life Technologies) supplemented with 10% heat-inactivated fetal bovine serum (FBS ...
-
bioRxiv - Biochemistry 2022Quote: ... Streptavidin solution (5 µg/mL) was plated in 50µL in each well on a MaxiSorp (Thermo Fisher Scientific) microtiter plate in 50 mM sodium bicarbonate pH 8.75 ...
-
bioRxiv - Neuroscience 2021Quote: ... washed once in TN buffer for 5 min and mounted with Fluoromount-GTM mounting medium with DAPI (Invitrogen).
-
bioRxiv - Neuroscience 2021Quote: ... and plasmidic DNA (0.9µg/µL) in a 1:5 ratio were separately pre-incubated with Opti-MEM (Gibco). Lipofectamine 2000 and plasmidic DNA were left to complex for 20 minutes at room temperature and added to the plated BV2 cells in low glucose DMEM supplemented with 5% fetal bovine serum (FBS ...
-
bioRxiv - Cell Biology 2020Quote: ... 3-5 μl of purified γ-TuRC was visualized on an SDS-PAGE with SYPRO Ruby stain (ThermoFisher) following the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2020Quote: ... low ROX (KAPA) was used for qPCR and data were acquired on a QuantStudio 5 (Thermo Fisher Scientific) and analyzed in Prism v8 (GraphPad).
-
bioRxiv - Molecular Biology 2020Quote: ... 5% CO2 for 24 h before transient transfection with plasmids encoding Affimer-His constructs using Lipofectamine 2000 (ThermoFisher), as per the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2021Quote: ... or empty vector (pcDNA 5) together with scrambled or Rbfox2 specific siRNAs using Neon Nucleofection System (Thermofisher Scientific) as described previously (Verma et al ...
-
bioRxiv - Cell Biology 2022Quote: ... 3T3L1 cells and Lenti-X 293T cells were cultured at 37 °C with 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM, Invitrogen) containing 10% fetal bovine serum (HyClone ...
-
bioRxiv - Cell Biology 2022Quote: ... INS-1 cells were transfected in 6-well plates with 5 nM siRNA using Lipofectamine RNAiMAX (Invitrogen, 13778030) and typically analyzed after 96 hours ...
-
bioRxiv - Cell Biology 2022Quote: ... seeded on t25 flasks at 37°C 5% CO2 in DMEM/F12 (1:1) (ThermoFisher Scientific, Cramlington, UK) with 10%FBS ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells were maintained at 37°C under 5% CO2 in the following culture medium: DMEM (Dulbecco’s modified Eagle’s medium) Glutamax (InVitroGen) containing 10% of heat inactivated fetal bovine serum ...
-
bioRxiv - Immunology 2022Quote: ... supplemented with 5% heat-inactivated fetal bovine serum (FBS) and 1X Penicillin-Streptomycin solution (Thermo Fisher Scientific, USA). SARS-CoV-2 clinical isolates (D614G variant ...
-
ArchR: An integrative and scalable software package for single-cell chromatin accessibility analysisbioRxiv - Genomics 2020Quote: ... MCF10A cells were ordered from ATCC and cultured in DMEM/F12 containing 5% horse serum (Thermo Fisher 16050130), 0.02 ug/ml human EGF (PeproTech AF-100-15) ...
-
bioRxiv - Genomics 2019Quote: ... and supplemented with 5% fetal bovine serum (FBS) and 250 μg/ml Geneticin G418 (ThermoFisher Scientific, MA, USA). For differentiation ...
-
bioRxiv - Genomics 2020Quote: ... Real-time quantitative polymerase chain reaction (PCR) was performed on QuantStudio 5 Real-Time PCR Instrument (Applied Biosystems). All experiments were done in triplicate with SYBR-GreenER pPCR SuperMix (Life Technologies) ...
-
bioRxiv - Immunology 2021Quote: ... we measured the proliferation capacity of NK92 cells by an EdU (5- ethynyl-2’-deoxyuridine; Invitrogen, California, USA) proliferation assay ...
-
bioRxiv - Microbiology 2020Quote: ... Cells were stained with DAPI at 5 μg/ml and mounted with Slow-Fade mounting medium (Molecular Probes) or Prolong Diamond (Thermo Fisher Scientific).
-
bioRxiv - Neuroscience 2019Quote: Individual 5 dpf larvae were placed into each well of a 24-well tissue culture plate (Fisher Scientific) and transferred into a Zebrabox imaging chamber (Viewpoint) ...
-
bioRxiv - Immunology 2019Quote: ... A maximum of 5×103 live CD3+CD4+ T cells was sorted into 100 μL of RNAlater (Ambion) using a FACSAria III (BD Biosciences) ...
-
bioRxiv - Biochemistry 2019Quote: ... Reduction of disulfide bonds was performed with 5 mM Tris(2-carboxyethyl)phosphine (TCEP) Bond-breaker (Thermo Scientific) at RT for 1 h ...
-
bioRxiv - Immunology 2019Quote: ... counted and plated at a density of 5×105 cells/well (24 well-plates) in RPMI 1640 (Gibco) 10% FBS (Dutcher) ...