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Citations for Thermo Fisher :
7001 - 7050 of 10000+ citations for 5 Nitrofluorescein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... Samples were heated for 5 min at 90°C in 1X Novex TBE-Urea Sample Buffer (Invitrogen), subjected to 12% 7.5 M Urea PAGE ...
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bioRxiv - Biochemistry 2019Quote: All cells were grown at 37°C and 5% CO2 and cultured in DMEM (Thermo Fisher Scientific) containing 1X GlutaMAX (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2019Quote: ... were maintained at 37 °C within a (5% CO2) Dulbecco’s Modified Eagle Medium (Life Technologies, Zug, Switzerland) containing 4500 g/mL glucose ...
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bioRxiv - Molecular Biology 2020Quote: ... samples were desalted using HyperSep Filter Plates with a 5-7 μL bed volume (Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... GST or GST-EB1 (5 μg) were incubated with 20 μl glutathione agarose beads (16100; Thermo Scientific) for 2 h at 4°C ...
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bioRxiv - Biochemistry 2019Quote: ... To crosslink the antibody to the beads 0.5 µL of 250 mM BS(PEG)5 (Thermo scientific) (dissolved in dry DMSO ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by gel size selection of 5′ adaptor ligated RNAs and reverse transcription (SuperScript III RT, Invitrogen) with 3′ random priming ...
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bioRxiv - Cell Biology 2020Quote: For Immunofluorescence staining 12 µm frozen sections were rehydrated for 5 min with PBS (Thermo Fisher, 10010056) followed by an ethanol gradient each 30 sec (70 % ...
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bioRxiv - Genomics 2021Quote: ... RNA was chemically fragmented for 5 min at 70°C with RNA Fragmentation Reagents (Thermo Fisher Scientific), followed by DNase I treatment (Roche) ...
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bioRxiv - Genetics 2021Quote: ... colonies were washed with PBS and dissociated to single cells with a 5 min trypsin (Life technologies) incubation ...
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bioRxiv - Genetics 2020Quote: ... cells were passaged into differentiation media supplemented with 5-Ethynyl-2’-deoxyuridine (Life Technologies, A10044, 10 µM). On day 6 of differentiation ...
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bioRxiv - Cell Biology 2021Quote: ... and then cut at a 5-μm thickness using a cryostat microtome (Cryotome FSE, Thermo Fisher Scientific). We executed immunostaining on testicular cryosections with primary antibody (anti-TCFL5) ...
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bioRxiv - Cell Biology 2019Quote: ... actin was incubated with 3-5 molar excess amount of Alexa Fluor 488 C5 maleimide (Invitrogen, #A10254) or EZ-Link Maleimide-PEG2-Biotin (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: hTERT-RPE1 cells (ATCC) were maintained at 37°C with 5% CO2 in MEM-α (Life Technologies) containing 10% FBS (Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were left to invade for 5 days before staining with 4μM Calcein AM (Invitrogen, #C1430). Z-stack images were captured every 10μm using the Olympus FV1000 confocal microscope with the UplanSApo 20x/0.74 objective ...
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Adaptive translational pausing is a hallmark of the cellular response to severe environmental stressbioRxiv - Molecular Biology 2020Quote: ... 300 μL of the combined fractions was digested with 5 μL RNase I (Thermo Fisher Scientific, AM2295) for 1 h at 4°C (slow rotation on the nutator) ...
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bioRxiv - Neuroscience 2021Quote: Rats were given a .5 μl volume infusion of fluorophore-conjugated muscimol (BODIPY TMR-X; Life Technologies) 30 min before perfusion (Allen et al. ...
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bioRxiv - Immunology 2021Quote: ... Intracellular cytokine staining was performed after incubation for 5 hours with Remel™ PHA Purified (ThermoFisher Scientific) in the presence of 2 µg/ml Brefeldin ...
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bioRxiv - Biochemistry 2020Quote: HEK 293 cells were cultured in 95% air and 5% CO2 in Dulbecco’s modified Eagle’s medium (Gibco) supplemented with 10% fetal bovine serum (Gibco ...
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bioRxiv - Biochemistry 2021Quote: ... and the supernatant was applied to a 5 mL HisTrap Ni-NTA Crude column (Thermo Fisher Scientific). The column was washed with His binding buffer ...
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bioRxiv - Immunology 2021Quote: ... the purified cells were cultured in RPMI 1640 supplemented with 5 % Foetal Bovine Serum (both Thermofisher, UK).
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bioRxiv - Cancer Biology 2021Quote: ... Cells were cultured at 37°C in 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM, Life Technologies) supplemented with 10% FBS (Life Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... Real-time qPCR was performed using Applied biosystem Quant studio 5 real time PCR System (Thermo Scientific). Expression data of the studied genes was normalized to RPLP1 to control the variability in expression levels and were analyzed using the 2-ΔΔCT method described by Livak and Schmittgen (78) ...
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bioRxiv - Immunology 2021Quote: ... Reactions were conducted with 5 μL RNA and TaqMan Fast Virus 1-Step Master Mix (Applied Biosystems) with 500 nM primers and 200 nM probes ...
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bioRxiv - Cell Biology 2021Quote: ... Permeabilized cells were washed with PBS (2x) and blocked with 5% goat serum (Invitrogen, Grand Island, NY) for 45 minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... 5 SCID tumor samples and the mT3-2D cell line using the PureLink RNA Mini Kit (Ambion). RNA concentration and quality were assessed using the Agilent BioAnalyzer ...
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bioRxiv - Developmental Biology 2021Quote: ... incubated with DAPI for 5 min in PBS and washed twice before mounting with Prolong Gold (Invitrogen). Cells were imaged on a Zeiss Imager.Z2 microscope using the ApoTome.2 structured illumination platform ...
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bioRxiv - Developmental Biology 2021Quote: ... Japanese quail skins were incubated at 37°C with a 5% CO2 atmosphere (Thermo Scientific Midi 40). Explant were cultured at 37°C in 5% CO2 atmosphere (Thermo Scientific Midi 40) ...
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bioRxiv - Cell Biology 2019Quote: MCF10A human mammary epithelial cells (ATCC) were grown at 37° and 5% CO2 in DMEM/F12 (Gibco) with 5% horse serum (HS ...
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bioRxiv - Genetics 2021Quote: ... 5 U of the required restriction enzyme (BsaI/Eco31l, #ER0292, Thermo Fisher, or BpiI, ER1011, Thermo Fisher), and 400 U T4 DNA ligase (M0202 ...
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bioRxiv - Genetics 2021Quote: ... 5 U of the required restriction enzyme (BsaI/Eco31l, #ER0292, Thermo Fisher, or BpiI, ER1011, Thermo Fisher), and 400 U T4 DNA ligase (M0202 ...
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bioRxiv - Genetics 2021Quote: ... The reaction was performed using SYgreen Blue Mix (PCRbio # PB20.15-20) and a QuantStudio 5 (Applied Biosystems) thermal cycler.
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bioRxiv - Genetics 2019Quote: 2.106 CEM cells were centrifuged for 5 minutes at 1200 rpm and washed in PBS 1X (Gibco). They were then transfected by nucleofection with 5 μg DNA in 100 μl of Cell Line Nucleofector Solution V (Lonza ...
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bioRxiv - Cancer Biology 2021Quote: ... depending on the sorting round) and 5 µg/mL mouse anti-c-myc (clone 9E10, Thermo Fisher), followed by a secondary incubation with 20 µg/mL goat anti-mouse IgG-Alexa Fluor 488 and 10 µg/mL streptavidin-Alexa Fluor 647 (both Thermo Fisher) ...
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bioRxiv - Microbiology 2021Quote: ... peptides were sampled on a 300 μm × 5 mm PepMap C18 precolumn (Thermo Scientific, Illkirch-Grafenstaden/France) and separated on a 75 μm × 250 mm C18 column (Reprosil-Pur 120 C18-AQ ...
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bioRxiv - Microbiology 2020Quote: ... Glycosylated RboP hexamers were coupled to beads by adding 5×107 Dynabeads M280 Streptavidin (Thermo Fisher Scientific) to the individual glycosylation reaction mixtures ...
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bioRxiv - Microbiology 2020Quote: ... MEFs were maintained at 5% CO2 and 37°C in high-glucose Dulbecco’smodified Eagle’s medium (DMEM, Gibco) supplemented with 10% (v/v ...
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bioRxiv - Microbiology 2020Quote: ... 5 µl of sample was loaded onto the trap using an Easy nLC-1000 (Thermo Fisher Scientific). Each sample was separated using the Easy nLC-1000 with a binary mobile phase gradient to elute the peptides ...
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bioRxiv - Molecular Biology 2021Quote: 5-ethynyl-2’-deoxyuridine (EdU)-incorporation assay has been performed using a commercially available kit (Invitrogen, C10340). Animals were injected intraperitoneally with 60 µl of EdU (0.5 mg/ml in 50% DMSO and 50% PBS solution ...
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bioRxiv - Immunology 2020Quote: ... T cells were resuspended in 1X PBS and stained with CFSE (Invitrogen, #C34554, working concentration: 5 μM). T cells were incubated at 37°C for 20 mins and then washed twice with an excess of media ...
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bioRxiv - Microbiology 2021Quote: ... The 10-μL PCR mixture comprised 5 μL of TaqMan® Universal Master Mix II (Applied Biosystems) with uracil-N-glycosylase (UNG) ...
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bioRxiv - Microbiology 2021Quote: ... U937 monocyte cells were cultivated at 37°C in 5 % CO2 in RPMI 1640 medium (ThermoFisher Scientific) supplemented with 10 % heat-inactivated foetal calf serum (HyCloneTM) ...
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bioRxiv - Immunology 2020Quote: For cell proliferation assays splenocytes freshly isolated from P14 mice were labelled with 5 μM CFSE (Invitrogen) at 37 °C for 20 min in RPMI complete media (described above) ...
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bioRxiv - Immunology 2020Quote: Human 293F cells were maintained at 37°C with 5% CO2 in FreeStyle 293 Expression Medium (ThermoFisher) supplemented with penicillin and streptomycin ...
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bioRxiv - Immunology 2020Quote: ... Lipid droplets and nuclei were stained with 0.2% Triton-X100/PBS containing 4,4-difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene (BODIPY 493/503, 5 μg/ml; Thermo Fisher)88 ...
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bioRxiv - Immunology 2020Quote: ... Samples were acquired on an LSRFortessa 5 with stained UltraComp eBeads Compensation Beads (Invitrogen #01-2222-41) as compensation controls.
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bioRxiv - Developmental Biology 2021Quote: ... received two intraperitoneal (i.p.) injections of 5-ethynyl-2’-deoxyuridine (EdU) (Thermo Fisher Scientific, Cat. No.: A10044) 1mg each and 4 hours apart ...
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bioRxiv - Genomics 2020Quote: ... (RV-5′-GCCGTGGGTACTGAGAAAG-3′) and the fragments were bi-directional sequenced using (3130 Genetic Analyser; Applied Biosystems). Further the results were compared with the reference sequence of ARHGEF40 gene using NCBI-BLAST program and the chromatogram was analysed using Chromas lite (2.1 ...
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bioRxiv - Cell Biology 2021Quote: ... (5 μg with Takara Clontech Xfect in NRVMs, 2.5 ug and 7.5ul Lipofectamine LTX reagent, Thermo Fisher’s Lipofectamine LTX & PLUS Reagent ...
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bioRxiv - Genetics 2020Quote: ... 5% CO2 in Dulbecco modified Eagle medium (DMEM; GE) supplemented with 10% fetal bovine serum (FBS; GIBCO) and 1% antibiotics (penicillin 100 U/ml ...