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Citations for Thermo Fisher :
651 - 700 of 10000+ citations for GATOR2 complex protein WDR24 WDR24 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: The IN/IBD complex cryo-EM dataset was collected on a Titan Krios transmission electron microscope (Thermo Fisher Scientific) operating at 300 keV ...
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bioRxiv - Biochemistry 2023Quote: ... The S-GSAS/6P:R1-26 complex cryo-grid was imaged in a Titan Krios electron microscope (Thermo Fisher Scientific) operating at 300 kV and equipped with Gatan BioQuantum energy filter (slit width 20 eV ...
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bioRxiv - Biophysics 2023Quote: ... 110µl TRAK1-KIF5B complex eluted from the beads were incubated with 1.2mM bis(sulfosuccinimidyl)suberate (BS3) (Thermo Fisher Scientific) for 30min at 4°C ...
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bioRxiv - Cell Biology 2022Quote: Cryo-EM images of the TFEB-Rag-Ragulator complex were recorded under a Talos Arctica microscope (Thermo Fisher Scientific) operated at 200 kV ...
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bioRxiv - Genomics 2022Quote: ... For each experiment LNCaP-dCas9-KRAB cells were transfected with 30 nM sgRNA complex using Lipofectamine RNAiMAX (Invitrogen, 13778030). 48 hours post-transfection cells were treated with media containing 10 µM enzalutamide or DMSO for a further 24 hours.
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bioRxiv - Biophysics 2022Quote: ... for 24 h and then transiently transfected with 2 μg of control or mutant plasmid expressing the human R-type CaV2.3 calcium channel complex (CaV2.3 α1E, β1, α2δ1) using 1.2 μg of Lipofectamine 2000 Reagent (Thermo Fisher Scientific, USA). Patch-clamp experiments were performed 12 to 24 hours post-transfection at room temperature (21∼25°C ...
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bioRxiv - Molecular Biology 2024Quote: 5 × 105 K562 cells were transfected with the RNP complex using the Neon Transfection System (Thermo Fisher Scientific, #MPK5000). Cells were than cultured for 24 hours in RPMI-1640 supplemented with 10% FBS before fluorescence activated cell sorting ...
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bioRxiv - Genomics 2024Quote: ... The Cas9 RNP complex was generated following IDT’s recommendations and transfected into Jurkats using the Neon Electroporation System (Invitrogen). To isolate clones ...
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bioRxiv - Immunology 2024Quote: ... The crRNA:tracrRNA complex was ensembled following the manufacturer’s protocol and delivered by electroporation using the Neon Transfection System (Thermo Fisher).
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bioRxiv - Genomics 2024Quote: ... The bTMP−DNA complex in TE was immunoprecipitated using avidin conjugated to magnetic beads (Dynabeads MyOne Streptavidin Invitrogen, 65001) overnight at 4 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... The obtained complex and input DNA were decrosslinked in TE buffer with 1% SDS and proteinase K (Invitrogen, 25530049) at 65°C for overnight ...
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bioRxiv - Microbiology 2020Quote: ... followed by incubation with rabbit antibodies against SFTSV-N as primary antibodies and HRP-conjugated recombinant protein A/G (Cat. No. 32490, Thermo Scientific, Rockford, IL, USA) as secondary antibodies ...
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bioRxiv - Biophysics 2023Quote: ... The company provided affinity-purified antibodies that we validated by immunoprecipitating Scm3 from yeast strains with Scm3-V5 and confirming that the antibody recognized a protein of the correct molecular weight that was also recognized by α-V5 antibody (Invitrogen; R96025; 1:5000). We subsequently used the antibody at a dilution of 1:10,000 ...
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bioRxiv - Bioengineering 2024Quote: ... PCs and ACs were stained with neural/glial antigen 2 (NG-2) antibody (eBioscience, 14-6504-80, 1:100) and glial fibrillary acidic protein (GFAP) antibody (Invitrogen, PA1-10019, 1:1000), respectively ...
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bioRxiv - Cell Biology 2024Quote: ... followed by confirmation of antibody recognition a protein of the correct molecular weight that was also recognized by α-V5 antibody (Invitrogen; R96025; 1:5000) or α-myc antibody (Cell Signaling Technology ...
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bioRxiv - Microbiology 2023Quote: ... protein concentrations were determined by Bradford Protein Assay protein using Coomassie protein assay reagent (Thermo Fisher Scientific-Invitrogen ...
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bioRxiv - Developmental Biology 2021Quote: ... 25% formamide, 2X SSC, 1 mg/mL E.coli tRNA, 1 mM vanadyl ribonucleoside complex, 0.25 mg/mL BSA; Thermo Fisher Scientific). Samples were washed twice for 30 min at 30 °C with wash buffer (25% formamide ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated with Zenon Alexa Fluor 555 rabbit IgG labeling kit (according to the instructions for Zenon complex formation [Z25305; Invitrogen]) using anti-DsRed (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... sections were washed and incubated with either biotinylated (1:200 followed by avidin-biotin complex (ABC) amplification and 3,3-diaminobenzidine (Thermo scientific) or fluorescent secondary antibody to visualize proteins ...
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bioRxiv - Immunology 2021Quote: ... added to the Cas9/sgRNA complex and electroporated using 20-ms pulse at 1725 V using the Neon Transfection System (Invitrogen). The cleavage efficiency of sgRNA was about 60% as assessed by Synthego ICE CRISPR software after PCR amplification and sequencing of the targeted genomic DNA ...
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bioRxiv - Cancer Biology 2020Quote: The PRMT5:WDR77 complex was co-expressed in Sf9 cells using the dual promoter pFastBac Dual baculovirus system (Thermo Fisher). Protein purity and molecular mass were confirmed by SDS-Page Coomassie staining and LC/MS ...
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bioRxiv - Cell Biology 2022Quote: ... were used at 1:10,000 dilution to detect the bait-prey complex and blot was developed by Super signal west pico chemiluminescent substrate (Thermofisher).
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bioRxiv - Cancer Biology 2022Quote: ... The transfection of the gRNA and Cas9 endonuclease was achieved by assembly and delivery of an RNP complex via electroporation using the Neon Transfection System Kit (Invitrogen). The RNP complex was assembled at 1:2 molar ratio of TrueCut™ Cas9 Protein v2 (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... Myc-Arhgef18 and/or the septin complex (Flag-SEPTIN2, Flag-SEPTIN6, Flag-SEPTIN7, GFP-SEPTIN9) on 6-well tissue-culture plates using Lipofectamine 3000 (Invitrogen) and harvested 18–24 h later ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then transfected with TransIT-LT1 Reagent-plasmid DNA complex containing 250 μl of Opti-MEM Reduced-Serum Medium (Gibco), 2.5 μg plasmid DNA and 7.5 μl TransIT-LT1 Reagent and incubated for 72 h ...
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bioRxiv - Neuroscience 2021Quote: ... sections were washed and incubated with either biotinylated (1:200 followed by avidin-biotin complex (ABC) amplification and 3,3-diaminobenzidine (Thermo Scientific) or fluorescent secondary antibodies with species-specific Alexa Fluor-488 or -568 (Invitrogen A-11039 or A-11011 ...
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bioRxiv - Biochemistry 2021Quote: The KDELR/Legobody complex at 0.8mg/ml was PEGylated by incubation with MS(PEG)12 methyl-PEG-NHS-ester (Thermo Fisher) at a 1:40 molar ratio for 2 hrs on ice to reduce preferred particle orientation on the grids ...
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bioRxiv - Cancer Biology 2023Quote: The 387 complex genetics sarcomas were analyzed on Human Genome U133 Plus 2.0 array (900466, Affymetrix, Santa Clara, CA, USA), according to the manufacturer’s procedures ...
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bioRxiv - Neuroscience 2022Quote: ... The eluted complex was magnetically separated from the beads and transferred in a 384-well plate (Nunc catalog #264573; Sigma) where it was neutralized with 10 μL/well of Erenna buffer D (catalog #02-0368-00 ...
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bioRxiv - Biophysics 2023Quote: Cryo-EM data collection for the hCLDN-4/cCpE/COP-1 complex was performed on a Titan Krios G3i (ThermoFisher) equipped with a Gatan K3 direct electron detector and BioContinuum GIF at PNCC ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were then washed with PBS and immersed in the solution containing the avidin- biotin-peroxidase complex (Ultra-Sensitive ABC Peroxidase Standard Staining Kit, ThermoFisher) for 30 min at RT ...
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bioRxiv - Pathology 2023Quote: ... The Smn and scrambled siRNAs were aliquoted separately into an siRNA-lipofectamine complex containing Lipofectamine® RNAiMAX Reagent (#13778075, Invitrogen) and Opti-MEM (#31985062 ...
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bioRxiv - Biophysics 2023Quote: Cryo-EM data collection for the hCLDN-4/cCpE/COP-1/Nb complex was performed on a Titan Krios G3i (ThermoFisher) equipped with a Gatan K3 direct electron detector and BioQuantum GIF at the University of Chicago Advanced Electron Microscopy Core Facility (RRID:SCR_019198) ...
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bioRxiv - Biochemistry 2023Quote: Plasmids encoding each subunit of the BAM complex and OmpC genes (including a His6-tag) were transformed into BL21(DE3)* cells (Invitrogen). BamD and OmpC variant transformants were grown in LB (+ amp ...
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bioRxiv - Cell Biology 2023Quote: ... 15,000 cells were reverse-transfected with 0.3μl of Lipofectamine RNAiMax complex containing either 1 pico mol Silencer siRNA targeting or control non-targeting siRNA (Invitrogen 4390843). Media was exchanged 24 hours later and gene expression was assessed beginning 48 hours later in flow cytometry time courses or used as an endpoint for western blots ...
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bioRxiv - Cell Biology 2023Quote: The baculovirus preparation for recombinant human HAUS complex expression was carried out according to the manufacturer’s protocol (Bac-to-Bac system, Life Technologies) using E ...
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bioRxiv - Cell Biology 2024Quote: ... plasmid (EV or HLA-A2/Kb; 1 µg/µL) complex at 2:1 ratio prepared in Opti-MEM (Gibco, UK) for 18 hours in the presence or absence of 100 ng/mL type I IFNα (PBL Assay Science ...
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bioRxiv - Genomics 2024Quote: ... The mRNA-cDNA complex was collected from the beads and further purified using Dynabeads MyOne Streptavidin C1 beads (Thermo Fisher). The streptavidin beads were first washed three times with binding buffer containing 10 mM Tris-HCl pH 7.5 ...
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bioRxiv - Microbiology 2024Quote: Purified PmSLP-1:FI complex prepared in PBS pH 7.4 was cross-linked with disuccinimidyl suberate (DSS, Thermo Fisher Scientific). Briefly ...
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bioRxiv - Microbiology 2024Quote: ... 5 µl of amplification reaction was mixed with 10 µl of the crRNA/Cas12a complex in CRB2 buffer (Table S1) in a black 96-well plate (Cat #23710, ThermoFisher), in a volume of 100 µl ...
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bioRxiv - Molecular Biology 2024Quote: ... The elution of the complex was done via cleavage of the TAP-tag by incubation with the AcTEV Protease (Invitrogen) for 3 h at 17 °C in Buffer 30.
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bioRxiv - Plant Biology 2020Quote: ... To detect V5-tagged proteins a 1: 5,000 dilution of the primary anti-V5 antibody (Invitrogen, #R96025) and an anti-mouse horseradish peroxidase-conjugated secondary antibody (1:10,000 dilution ...
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bioRxiv - Cell Biology 2020Quote: ... or anti-goat RFC1 antibody (2 μg/sample) coupled to Protein G agarose beads (Thermo Fisher Scientific) for 2 h on an end-over-end rotator at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: 1μl Mouse anti-V5 antibody was added to every 10μl Dynabeads™ protein G (Thermo Fisher Scientific) (2.5μl per sample ...
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bioRxiv - Genetics 2021Quote: ... Equal amounts of cell lysates were incubated with the indicated antibodies conjugated to protein G beads (Invitrogen) respectively from 4h to overnight at 4° C ...
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Molecular coevolution of nuclear and nucleolar localization signals inside basic domain of HIV-1 TatbioRxiv - Evolutionary Biology 2021Quote: ... The antibody-bound proteins were detected using Pierce ECL western blotting substrate (Thermo Scientific, Waltham, MA, USA), and images were acquired using a Gel DocXR system (Bio-Rad ...
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bioRxiv - Bioengineering 2021Quote: ... and probed with SARS-CoV/SARS-CoV-2 Spike Protein S2 Monoclonal Antibody (1A9) (ThermoFisher # MA5-35946) and donkey anti-mouse secondary antibodies tagged with an infrared fluorophore (Rockland Immunochemicals) ...
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bioRxiv - Biochemistry 2021Quote: ... Antibody-bound complexes were immunoprecipitated with Novex DYNAL Dynabeads Protein G (Invitrogen, cat. no. 10-003-D) or protein A/G agarose beads (EMD Millipore ...
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bioRxiv - Neuroscience 2021Quote: ... The antibody-HTT complexes were precipitated with protein G covalently conjugated to magnetic beads (Thermo Scientific, P188803). The HTT-depleted lysates were seeded with 10 ng of sonicated HTTex1 fibrils for 4 hr at 25°C with rotation ...
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bioRxiv - Microbiology 2020Quote: ... and 40 µg of antibody was bound to 3 mg of protein G Dynabeads (Thermo Fisher Scientific) in 400 µL of PBS buffer containing 0.2% (v/v ...