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Citations for Thermo Fisher :
701 - 750 of 10000+ citations for GATOR2 complex protein WDR24 WDR24 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... was covalently coupled to magnetic beads at 12 μg antibody per 100 μL Dynabeads Protein A (ThermoFisher). Around 1500 μg of cell lysate and 10 μg of antibody was used per sample ...
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bioRxiv - Genomics 2022Quote: ... The antibody was added to 5μg of sonicated chromatin along with Dynabeads Protein G magnetic beads (Invitrogen) and incubated at 4°C overnight ...
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bioRxiv - Biochemistry 2022Quote: ... The total protein level was detected by primary anti-His-tag antibody (1:10000, MA1-21315, Invitrogen) and secondary anti-mouse antibodies (1:5000 ...
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bioRxiv - Systems Biology 2022Quote: ... Antibody/chromatin complexes were captured with ChIP-grade protein A/G magnetic beads (Thermo Fisher, cat # 26162) for 2 h at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... and the antibodies were purified using NAb protein A plus spin kit (Thermo Fisher Scientific, Cat# 89948) according to the manufacturer’s protocol.
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bioRxiv - Neuroscience 2021Quote: ... Myelin detection was done with antibodies against myelin basic protein (MBP; rabbit, 1:4000; Invitrogen, RRID: AB_1501419) and proteolipid protein (PLP ...
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bioRxiv - Microbiology 2020Quote: ... IP’s were performed using 15 μg of each antibody with 50 μL Protein G Dynabeads (ThermoFisher Scientific), for 8 hours at 4°C on rotation ...
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bioRxiv - Cancer Biology 2022Quote: ... Chromatin bound to the antibody was then immunoprecipitated using 25ul of protein A Dynabeads (Thermo Scientific; 10002D) for 2h with rotation at 4 °C ...
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bioRxiv - Developmental Biology 2022Quote: ... The protein-antibody-sepharose mixture was then washed 5 times in Pierce IP lysis buffer (ThermoFisher Scientific) and resuspended in 2X Laemmli sample buffer (Sigma) ...
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bioRxiv - Microbiology 2021Quote: ... The His-tagged proteins were blotted using anti-6x His tag HRP-conjugated monoclonal antibody (Invitrogen, USA) and detected using Clarity Western ECL Substrate (Bio-Rad ...
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bioRxiv - Developmental Biology 2020Quote: ... The supernatant was incubated with anti-GFP antibody (5 μg/ml) prebound to Dynabeads Protein A (Invitrogen) for 2h with end-over-end rotation to capture GFP-fused L10a subunit of the 60S ribosome ...
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bioRxiv - Biochemistry 2020Quote: ... The expression of the recombinant protein was measured by ELISA using anti‐His tag monoclonal antibody (Invitrogen) to capture and biotinylated anti‐ANG1 antibody for detection (R&D System) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... followed by binding of antigen antibody complexes to protein A/G magnetic beads (Thermo scientific, Rockford, USA) for 1 h ...
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bioRxiv - Biochemistry 2021Quote: ... and transferrin using the High Select Top 14 Abundant Protein Depletion Camel Antibody Resin (Thermo Fisher Scientific). On the day of depletion ...
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bioRxiv - Molecular Biology 2021Quote: Polyclonal antibodies used in IPs (α-Med17, α-Med3) were conjugated to Protein G Dynabeads (Life Technologies) in batch as follows ...
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bioRxiv - Immunology 2020Quote: ... Recombinant proteins and antibody 47D11 were expressed transiently in FreeStyle™ 293-F Cells (Thermo Fisher Scientific) and affinity purified from the culture supernatant by protein-A sepharose beads (GE Healthcare ...
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bioRxiv - Biophysics 2022Quote: ... The coverslips were stained with the following antibodies: rabbit anti-spike protein (Invitrogen; cat. 703959; 1:500) and mouse anti-dsRNA (J2 ...
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bioRxiv - Microbiology 2023Quote: DB1 antibody was purified by binding clarified cell supernatant to Protein A Agarose beads (Thermo Fisher Scientific), with elution into 100 mM glycine pH 3.0 and was quickly neutralised with 1 M Tris-HCl pH 8.0 ...
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bioRxiv - Biophysics 2022Quote: ... Protein bands were visualized through horseradish peroxidase (HRP) conjugated secondary antibody by ECL reagent (34577, ThermoFisher Scientific).
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bioRxiv - Microbiology 2024Quote: ... Tight junction and control proteins were detected using antibodies for ZO-1 (catalog number 339100, ThermoFisher Scientific), GAPDH (catalog number 2118 ...
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bioRxiv - Microbiology 2023Quote: ... before being incubated overnight at 4 degrees C with the antibodies bound to Protein A Dynabeads (Invitrogen). Samples were washed for 3 minutes each at 4 degrees C with low salt wash (0.01% SDS ...
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bioRxiv - Immunology 2023Quote: ... Hexahistidine-tagged proteins were then detected using mouse anti-6xHis tag antibody (Ma1-135, Invitrogen, Karlsruhe, Germany) as primary antibody and signals revealed by horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (ab6789 ...
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bioRxiv - Biochemistry 2023Quote: ... and rabbit anti-SARS-CoV-2 spike protein (RBD) polyclonal antibody (Thermo Fisher Scientific; catalog # PA5-114451).
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bioRxiv - Cancer Biology 2023Quote: ... 10 μg of the respective antibodies were bound to 100 μL of Protein A magnetic beads (ThermoFisher). Beads were blocked with three 1 mL washes of 5 mg/mL BSA in PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... Cleared lysates were incubated with anti-IGF2BP1 or anti-AGO2 antibody and Protein G Dynabeads (Life Technologies) for 30 min at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... primary antibodies for mouse anti-MAP2 (microtubule-associated protein 2; Invitrogen, Catalog No. 13-1500, 1:1000) and guinea pig anti-synapsin1/2 (Synaptic Systems ...
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bioRxiv - Molecular Biology 2023Quote: ... Antibody detection of the proteins was performed using the iBind Automated Western System (Thermo Fisher Scientific Inc.) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... and the antibodies were purified using NAb protein A plus spin kit (Thermo Fisher Scientific, Cat# 89948) according to the manufacturer’s protocol.
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bioRxiv - Neuroscience 2024Quote: Immunoprecipitation experiments were performed using various antibodies coupled with the Dynabeads Protein G system (Thermo Fisher 10004D), according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... Detection of protein-antibody complexes was performed using an enhanced chemiluminescence substrate (Thermo Fisher Scientific, USA, #32209) and digitally imaged with Azure 280 chemiluminescence detection system (Azure Biosystems ...
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bioRxiv - Immunology 2024Quote: ... and anti-glial fibrillary acidic protein (GFAP) antibody (rat IgG2a, 1:250, Invitrogen, #13-0300, Carlsbad, CA). The next day ...
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bioRxiv - Cancer Biology 2024Quote: ... The antibodies were labeled using an Alexa Fluor 488 (Alexa 488) Protein Labeling Kit from Thermo Fisher Scientific (A10235 ...
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bioRxiv - Cell Biology 2024Quote: ... Immunocomplexes in the antibody-lysate solutions were concentrated onto Pierce Protein A/G magnetic beads (Thermo Fisher) by incubating for 30 minutes at 4°C ...
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bioRxiv - Immunology 2024Quote: ... Cell proteins and EVs proteins were quantified by Qubit Protein Assay (Invitrogen) using a Qubit 3.0 Fluorometer (Thermo Fisher ...
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bioRxiv - Biophysics 2022Quote: ... A 50 μL fraction containing the largest molecular weight complex was assessed by SDS-PAGE (stained with SYPRO Red [Thermo Fisher]) and pooled for cryo-EM ...
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Metabolic function in aging retina and retinal pigment epithelium remains robust despite vision lossbioRxiv - Biochemistry 2021Quote: ... and the retina separated from the RPE-choroid-sclera complex (eyecup) in Hanks’ Balanced Salt Solution (Gibco; Grand Island, New York). Tissues for ATP determination were snap frozen and stored in liquid nitrogen ...
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bioRxiv - Microbiology 2021Quote: ... The gRNA:Cas9 ribonucleoprotein complex was assembled according to the manufacturer’s protocol (Integrated DNA Technologies) and reverse transfected using Lipofectamine RNAiMAX (Thermo Fisher Scientific) into ARN8 cells (3×105 cells in a 6-well plate) ...
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bioRxiv - Biochemistry 2022Quote: Purified homogenous Mad1:C-Mad2:O-Mad2 complex in the unphosphorylated or phosphorylated state were crosslinked with BS3 (bis[sulfosuccinimidyl] suberate) (ThermoFisher Scientific) before analysis by cryo-electron microscopy ...
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bioRxiv - Immunology 2022Quote: Pre-formed IL-27:IL-27Rα:GP130 complex sample was diluted to 0.1 mg/ml and vitrified in liquid ethane using Vitrobot Mark IV (Thermo Fisher Scientific). Lacey Carbon Au 300 grids (EMS ...
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bioRxiv - Biophysics 2021Quote: The ice-embedded LlCsm complex samples were collected on FEI Titan Krios electron microscope equipped with Gatan Bioquantum K3 direct electron detector (ThermoFisher Scientific) with the Leginon software for automatic data acquisition (47 ...
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bioRxiv - Immunology 2022Quote: ... Plat-E cells were cultured in a 96-well plate to 60-80% confluence and transfected with pMSCV-mCD4-PIG TCR vectors as a DNA-lipid complex using Lipofectamine 3000 (L3000001, ThermoFisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Lymph node cells from FNA were incubated with the RBD-fluorochrome complex and subsequently stained with aqua live/dead dye (Invitrogen, L34957), anti-human IgM (FITC ...
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bioRxiv - Immunology 2020Quote: ... CD8α+IEL were expanded in complete medium containing 50 ng/ml recombinant mouse IL-15/IL-15R complex (Thermo Fisher Scientific) on a tissue culture plate at the concentration of 5×105 cells/ml for 3 days ...
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bioRxiv - Immunology 2020Quote: ... THP-1 cells were electroporated with the sgRNA/Cas9 complex using the Neon transfection system (Thermo Fisher Scientific, Waltham, MA, USA) at 1600V ...
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bioRxiv - Microbiology 2021Quote: ... and the mRNA-linker-VHH complex was immobilized in 60 μL of Dynabeads MyOne streptavidin C1 magnetic beads (ThermoFisher Scientific, USA) at 25°C for 30 min ...
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bioRxiv - Neuroscience 2022Quote: ... The RNP complex and donor DNA was delivered to the cells by electroporation using the Neon Transfection System 100 μL kit (ThermoFisher, MPK10025) with Electrolytic-Buffer-E2 ...
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bioRxiv - Cell Biology 2022Quote: ... The complex was diluted in 263 μl prewarmed imaging media (low glucose (1g/L) DMEM without phenol red (Thermo Fisher, #11054020) supplemented with 10% FBS ...
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bioRxiv - Molecular Biology 2024Quote: ... The complex was incubated for 30 minutes at 4 ℃ before adding 10X CMC CHAPS (VitroEase™ Buffer Screening Kit, ThermoFisher Scientific) to a final concentration of 0.25X CMC ...
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bioRxiv - Molecular Biology 2024Quote: ... The complex was incubated for 30 minutes at 4 ℃ before adding 10X CMC CHAPS (VitroEase™ Buffer Screening Kit, ThermoFisher Scientific) to a final concentration of 0.5X CMC ...
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bioRxiv - Biochemistry 2023Quote: ... The S-GSAS/6P:H18:R1-32 complex cryo-grid was imaged in a 300 kV Titan Krios electron microscope (Thermo Fisher Scientific) equipped with a SelectrisX energy filter (slit width 10 eV ...